Possible involvement of protein kinases and Smad2 signaling pathways on osteoclast differentiation enhanced by activin A.


:Bone tissues reportedly contain considerable amounts of activin A and follistatin, an activin A-binding protein. In the present study, we found that follistatin strongly inhibited osteoclast formation in cocultures of mouse bone marrow cells and primary osteoblasts induced by 1alpha,25 dihydroxyvitamin D(3), prostaglandin E(2), and interleukin-1alpha. Antibody aganist activin A also inhibited the osteoclast formation. Furthermore, activin A synergistically stimulated osteoclast differentiation mediated by receptor activator NF-kappaB ligand (RANKL). RT-PCR analysis revealed that osteoblasts produced not only activin A but also follistatin. Western blot analysis of a panel of phosphorylated proteins revealed that activin A stimulated the phosphorylation of p44/42 mitogen activated protein (MAP) kinase (ERK1/2) and p38 MAP kinase in macrophage colony-stimulating factor-dependent bone marrow macrophages (M-BMMPhis). In addition, phosphorylation of Smad2 was observed in M-BMMPhis stimulated with activin A. These findings indicate that the phosphorylation of p44/42 MAP kinase, p38 MAP kinase, and Smad2 is involved in activin A-enhanced osteoclast differentiation induced by RANKL. Taken together, these results suggest that both activin A and follistatin produced by osteoblasts may play an important role in osteoclast differentiation through MAP kinases and Smad2 signaling pathways.


J Cell Physiol


Murase Y,Okahashi N,Koseki T,Itoh K,Udagawa N,Hashimoto O,Sugino H,Noguchi T,Nishihara T





Has Abstract


2001-08-01 00:00:00














  • Histomorphology and innate immunity during the progression of osteoarthritis: Does synovitis affect cartilage degradation?

    abstract::Osteoarthritis (OA) is a common chronic degenerative disease that affects all joints. At present, the pathological processes and mechanisms of OA are still unclear. Innate immunity, a key player in damage to the structure of the joint and the mechanism by which the host attempts to repair OA, affects all pathological ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wang H,Wang Q,Yang M,Yang L,Wang W,Ding H,Zhang D,Xu J,Tang X,Ding H,Wang Q

    更新日期:2018-02-01 00:00:00

  • Quercetin protects against radiocontrast medium toxicity in human renal proximal tubular cells.

    abstract::Radiocontrast media (RCM)-induced acute kidney injury (CI-AKI) is a major clinical problem whose pathophysiology is not well understood. Direct toxic effects on renal cells, possibly mediated by reactive oxygen species, have been postulated as contributing to CI-AKI. We investigated the effect of quercetin on human re...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Andreucci M,Faga T,Pisani A,Serra R,Russo D,De Sarro G,Michael A

    更新日期:2018-05-01 00:00:00

  • Regulated localization confers multiple functions on the protease urokinase plasminogen activator.

    abstract::We have investigated the role of the plasminogen activation cascade in skeletal muscle differentiation. Migrating, undifferentiated myoblasts express urokinase plasminogen activator (uPA) and its cell surface receptor (uPAR). Consequently, uPA is localized predominantly to the cell surface. Preventing uPA from associa...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wells JM,Strickland S

    更新日期:1997-05-01 00:00:00

  • The cancer-related transcription factor RUNX2 modulates expression and secretion of the matricellular protein osteopontin in osteosarcoma cells to promote adhesion to endothelial pulmonary cells and lung metastasis.

    abstract::Osteosarcomas are bone tumors that frequently metastasize to the lung. Aberrant expression of the transcription factor, runt-related transcription factor 2 (RUNX2), is a key pathological feature in osteosarcoma and associated with loss of p53 and miR-34 expression. Elevated RUNX2 may transcriptionally activate genes m...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Villanueva F,Araya H,Briceño P,Varela N,Stevenson A,Jerez S,Tempio F,Chnaiderman J,Perez C,Villarroel M,Concha E,Khani F,Thaler R,Salazar-Onfray F,Stein GS,van Wijnen AJ,Galindo M

    更新日期:2019-08-01 00:00:00

  • Characterization of a simian virus 40-transformed human podocyte cell line producing type IV collagen and exhibiting polarized response to atrial natriuretic peptide.

    abstract::Biology of glomerular visceral epithelial cells ("podocytes") and their role in inflammatory process remain obscure, partly because of the lack of well-differentiated podocyte cultures. We have established a human cell line by transfecting with a replication-defective SV40 plasmid (pSVHB1), a primary culture of podocy...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ardaillou N,Lelongt B,Turner N,Piedagnel R,Baudouin B,Estrade S,Cassingena R,Ronco PM

    更新日期:1992-09-01 00:00:00

  • Transmembrane potential responses during HL-60 promyelocyte differentiation.

    abstract::Myeloid cells, including granulocytes and monocyte/macrophages, are important in disease-associated inflammatory reactions. These cells come from a common progenitor, the promyelocyte. The human promyelocytic cell line, HL-60, can be induced to terminally differentiate into granulocytes or monocyte/ macrophages in a c...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Brent LH,Rubenstein B,Gong QH,Wieland SJ

    更新日期:1996-07-01 00:00:00

  • Adipose-derived stem cells and cancer cells fuse to generate cancer stem cell-like cells with increased tumorigenicity.

    abstract::Adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cells isolated from adipose tissue and have the ability to differentiate into adipogenic, osteogenic, and chondrogenic lineages. Despite their great therapeutic potentials, previous studies showed that ADSCs could enhance the proliferation and metastati...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chan YW,So C,Yau KL,Chiu KC,Wang X,Chan FL,Tsang SY

    更新日期:2020-10-01 00:00:00

  • miR-335-5p induces insulin resistance and pancreatic islet β-cell secretion in gestational diabetes mellitus mice through VASH1-mediated TGF-β signaling pathway.

    abstract::Multiple studies have reported different methods in treating gestational diabetes mellitus (GDM); however, the relationship between miR-335-5p and GDM still remains unclear. Here, this study explores the effect of miR-335-5p on insulin resistance and pancreatic islet β-cell secretion via activation of the TGFβ signali...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tang XW,Qin QX

    更新日期:2019-05-01 00:00:00

  • Upregulation of microRNA-27a inhibits synovial angiogenesis and chondrocyte apoptosis in knee osteoarthritis rats through the inhibition of PLK2.

    abstract::The function of microRNA-27a (miR-27a) on synovial angiogenesis and chondrocyte apoptosis in rats with knee osteoarthritis (KOA) by targeting PLK2 is explored in this present study. The rat model of KOA was conducted by anterior cruciate ligament transection. Rats were injected with miR-27a mimics, mimics NC, pcDNA3.1...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Liu W,Zha Z,Wang H

    更新日期:2019-12-01 00:00:00

  • Mechanical signals and IGF-I gene splicing in vitro in relation to development of skeletal muscle.

    abstract::It has been shown that the insulin-like growth factor (IGF-I) gene is spliced in response to mechanical signals producing forms of IGF-I which have different actions. In order to study how mechanical signals influence this gene splicing in developing muscle, C2C12 cells were grown in three-dimensional (3D) culture and...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cheema U,Brown R,Mudera V,Yang SY,McGrouther G,Goldspink G

    更新日期:2005-01-01 00:00:00

  • Is the secret for a successful aging to keep track of cancer pathways?

    abstract::A successful aging could be gained by life satisfaction, social functioning, or psychological resources and, definitely, by increasing resistance to diverse age-related pathologies. Nowadays, cancer can be considered an age-related disease since the incidence of most cancers increases with age, rising more rapidly beg...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Tramontano D,De Amicis F

    更新日期:2018-11-01 00:00:00

  • Expression, location, and interactions of ErbB2 and its intramembrane ligand Muc4 (sialomucin complex) in rat mammary gland during pregnancy.

    abstract::Muc4 (also called Sialomucin complex) is a heterodimeric glycoprotein complex consisting of a peripheral O-glycosylated subunit ASGP-1 (ascites sialoglycoprotein-1) tightly but non-covalently bound to an N-glycosylated transmembrane subunit ASGP-2. Muc4/SMC can act as an intramembrane ligand for ErbB2 via an EGF-like ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Price-Schiavi SA,Andrechek E,Idris N,Li P,Rong M,Zhang J,Carothers Carraway CA,Muller WJ,Carraway KL

    更新日期:2005-04-01 00:00:00

  • Interplay between the retinoblastoma related pRb2/p130 and E2F-4 and -5 in relation to JCV-TAg.

    abstract::Human polyomaviruses, which include JC virus (JCV) and BK virus (BKV), as well as the simian virus 40 (SV40), have been associated with human tumors and have been shown to be highly tumorigenic in experimental animal models. Although the mechanism by which JCV induces tumorigenesis is not entirely clear, earlier studi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Caracciolo V,Reiss K,Crozier-Fitzgerald C,De Pascali F,Macaluso M,Khalili K,Giordano A,Claudio PP

    更新日期:2007-07-01 00:00:00

  • Pathway of glutamate oxidation and its regulation in the HuH13 line of human hepatoma cells.

    abstract::Well-coupled mitochondria were isolated from a HuH13 line of human hepatoma cells and human liver tissue. The liver mitochondria showed a feeble glutamine oxidation activity in contrast to the hepatoma mitochondria, whereas they utilized glutamate well for the oxidative phosphorylation. In the liver mitochondria, glut...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Matsuno T

    更新日期:1991-08-01 00:00:00

  • Breast cancer-derived exosomes: Tumor progression and therapeutic agents.

    abstract::Tumor cells secrete extracellular vesicles (EVs) for intercellular communication. EVs by transporting different proteins, nucleic acids, and lipids contribute to affect target cell function and fate. ‎EVs which originate directly from multivesicular bodies so-called exosomes have dramatically fascinated the attention ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Jabbari N,Akbariazar E,Feqhhi M,Rahbarghazi R,Rezaie J

    更新日期:2020-10-01 00:00:00

  • Mechanism of action of amylin in bone.

    abstract::Amylin is a 37 amino acid peptide produced mainly by beta-cells of the endocrine pancreas. Human amylin has 43% homology with human calcitonin gene-related peptide (CGRP) and 13% homology with human calcitonin (CT). Amylin and CGRP have been reported to have CT-like hypocalcemic activity in vivo. To investigate the ro...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tamura T,Miyaura C,Owan I,Suda T

    更新日期:1992-10-01 00:00:00

  • The potential role of senescence in limiting fibrosis caused by aging.

    abstract::Fibrosis-related diseases carry with them a high mortality rate and their morbidity increases with age. Recent findings indicate that induced senescence in myofibroblasts can limit or reduce myocardial fibrosis, cirrhosis, and idiopathic pulmonary fibrosis, while also accelerating wound healing. However, more senescen...

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    pub_type: 杂志文章,评审


    authors: Meng X,Wang H,Song X,Clifton AC,Xiao J

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  • Differences of SiHa (human cancer of cervix) and BMG-1 (brain glioma) cell lines as 2D and 3D cultures.

    abstract::Cell cultures have seen much progress in the numbers available cell lines, their applications and culture techniques. Three dimensional cultures and co-cultures are examples of strategies that bring in vitro conditions closer to natural in vivo systems. We describe here, the formation of cell aggregates in three-dimen...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ravi M,Sah S,Bhammar R

    更新日期:2014-02-01 00:00:00

  • Synthesis and distribution of cytoskeletal elements in endothelial cells as a function of cell growth and organization.

    abstract::Confluent cultures of adult bovine aortic endothelial (ABAE), corneal endothelial (BCE), and fetal bovine heart endothelial (FBHE) cells form a monolayer of highly flattened, closely apposed, and nonoverlapping cells. In ABAE and BCE cultures, this is associated with a 50-fold decrease in the rate of DNA sythesis and ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Savion N,Vlodavsky I,Greenburg G,Gospodarowicz D

    更新日期:1982-02-01 00:00:00

  • Extra domain A-containing fibronectin expression in Spin90-deficient fibroblasts mediates cancer-stroma interaction and promotes breast cancer progression.

    abstract::Cancer-associated fibroblasts (CAFs) in the tumor microenvironment play major roles in supporting cancer progression. A previous report showed that SPIN90 downregulation is correlated with CAF activation and that SPIN90-deficient CAFs promote breast cancer progression. However, the mechanisms that mediate cancer-strom...

    journal_title:Journal of cellular physiology

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    authors: Kwon A,Chae IH,You E,Kim SH,Ahn SY,Lee OJ,Park ZY,Rhee S,Huh YH,Song WK

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    abstract::The effects of exposure of cultured P388D1 cells to H2O2 on intracellular free calcium ([Ca++]i) was investigated utilizing the intracellular fluorescent calcium chelator "Quin 2." [Ca++]i rose from approximately 150 nM to greater than 2 microM over a time course that was strongly dependent on the concentration of H2O...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hyslop PA,Hinshaw DB,Schraufstätter IU,Sklar LA,Spragg RG,Cochrane CG

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  • Lactate and TGF-β antagonistically regulate inflammasome activation in the tumor microenvironment.

    abstract::The tumor microenvironment significantly affects tumor progression, and tumor cells can also remodel the tumor microenvironment through complex interaction. Inflammasomes are innate immune system receptors/sensors that regulate an inflammatory response mainly mediated by the nucleotide-binding oligomerization domain-l...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tu CE,Hu Y,Zhou P,Guo X,Gu C,Zhang Y,Li A,Liu S

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  • Modulation of the in vitro angiogenic potential of human mesenchymal stromal cells from different tissue sources.

    abstract::Mesenchymal stromal cells (MSCs) have been widely exploited for the treatment of several conditions due to their intrinsic regenerative and immunomodulatory properties. MSC have demonstrated to be particularly relevant for the treatment of ischemic diseases, where MSC-based therapies can stimulate angiogenesis and ind...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Pinto DS,Ahsan T,Serra J,Fernandes-Platzgummer A,Cabral JMS,da Silva CL

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  • The protective role of peroxisome proliferator-activated receptor γ coactivator-1α in hyperthyroid cardiac hypertrophy.

    abstract::Heart failure is a major cause of death throughout the world. Hyperthyroidism has been shown to induce cardiac hypertrophy, which is a contributing factor to heart failure. However, the mechanism underling effect of thyroid hormone is not completely clear. The present study investigates the role of peroxisome prolifer...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xu W,Hou D,Jiang X,Lu Z,Guo T,Liu Y,Wang D,Zen K,Yu B,Zhang CY

    更新日期:2012-09-01 00:00:00

  • Glucose intolerance in monosodium glutamate obesity is linked to hyperglucagonemia and insulin resistance in α cells.

    abstract::Obesity predisposes to glucose intolerance and type 2 diabetes (T2D). This disease is often characterized by insulin resistance, changes in insulin clearance, and β-cell dysfunction. However, studies indicate that, for T2D development, disruptions in glucagon physiology also occur. Herein, we investigated the involvem...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Araujo TR,da Silva JA,Vettorazzi JF,Freitas IN,Lubaczeuski C,Magalhães EA,Silva JN,Ribeiro ES,Boschero AC,Carneiro EM,Bonfleur ML,Ribeiro RA

    更新日期:2019-05-01 00:00:00

  • LncRNA NEAT1 promotes extracellular matrix accumulation and epithelial-to-mesenchymal transition by targeting miR-27b-3p and ZEB1 in diabetic nephropathy.

    abstract::Diabetic nephropathy (DN) is a kind of microvascular complications of diabetes. Long noncoding RNAs (lnRNAs) can participate in the development of various diseases, including DN. However, the function of lncRNA NEAT1 is unclear. In our present study, we reported that NEAT1 was significantly increased in streptozotocin...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wang X,Xu Y,Zhu YC,Wang YK,Li J,Li XY,Ji T,Bai SJ

    更新日期:2019-08-01 00:00:00

  • Retinoic acid induces adult muscle cell differentiation mediated by the retinoic acid receptor-alpha.

    abstract::Retinoic acid (RA) is known to induce differentiation in many cell systems. This induction is mediated by nuclear RA receptors (RARs), which act as transactivating factors belonging to the superfamily of steroid/thyroid nuclear receptors. In this study, effects of RA were studied in a mouse myogenic C2 cell line and i...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Halevy O,Lerman O

    更新日期:1993-03-01 00:00:00

  • Pertussis toxin-sensitive G-proteins inhibit fibroblast growth factor-induced signaling in pancreatic acini.

    abstract::Signal transduction of fibroblast growth factor (FGF) receptors is known to involve tyrosine phosphorylation of several substrates, including Grb2, phospholipase C-gamma, and phosphatidylinositol 3-kinase, whereas the role of G-proteins in FGF receptor signaling is controversial. In the present study we investigated t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Piiper A,Stryjek-Kaminska D,Gebhardt R,Zeuzem S

    更新日期:1996-04-01 00:00:00

  • Myocilin is associated with mitochondria in human trabecular meshwork cells.

    abstract::The trabecular meshwork (TM) is a specialized tissue located at the chamber angle of the eye next to the cornea. This tissue is believed to be responsible for regulation of the aqueous humor outflow and control of the intraocular pressure (IOP). Alterations in functions of the TM may lead to IOP elevation and developm...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wentz-Hunter K,Ueda J,Shimizu N,Yue BY

    更新日期:2002-01-01 00:00:00

  • Over-expression of DEC1 inhibits myogenic differentiation by modulating MyoG activity in bovine satellite cell.

    abstract::Differentiated embryo chondrocyte 1 (DEC1), a member of basic-helix-loop-helix transcription factor Bhlhe40, also called stimulated by retinoic acid 13, STRA13, plays an important role in the regulation of adipogenesis, tumorigenesis, peripheral circadian output, response to hypoxia, and development of metabolic syndr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Huang Y,Lai X,Hu L,Lei C,Lan X,Zhang C,Ma Y,Zheng L,Bai YY,Lin F,Chen H

    更新日期:2018-12-01 00:00:00