Continuous maintenance of transformed fibroblasts under reduced serum conditions: utility as a model system for investigating growth factor-specific effects in nonquiescent cells.

abstract：:The binding of [3H]tuftsin to normal and in vivo stimulated mouse peritoneal macrophage populations was studied at 22 degrees C. The [3H]tuftsin binding to thioglycollate-stimulated macrophages was shown to be rapid and saturable, with an equilibrium dissociation constant (K(D)) (calculated from a Scatchard plot) of 5...

journal_title：Journal of cellular physiology

authors： Bar-Shavit Z,Stabinsky Y,Fridkin M,Goldman R

更新日期：1979-07-01 00:00:00

abstract：:Using S1 nuclease protection assays, we have examined the representation of cell cycle-dependent H4 histone RNAs in the nuclear matrix and nonmatrix nuclear fractions of human cells. Cytoplasmic and nuclear fractions were prepared from exponentially growing HeLa S3 cells by double detergent (sodium deoxycholate and NP...

journal_title：Journal of cellular physiology

authors： Bandyopadhyay R,Stein G,Stein J

更新日期：1986-08-01 00:00:00

abstract：:The purpose of this study was to evaluate the anti-head and neck squamous cell carcinoma (anti-HNSCC) cell activity by C6 ceramide and multikinase inhibitor PKC412. Experiments were performed on HNSCC cell lines (SQ20B and SCC-9) and primary human oral carcinoma cells. Results showed that PKC412 inhibited HNSCC cell p...

journal_title：Journal of cellular physiology

authors： Zhu Y,Wang C,Zhou Y,Ma N,Zhou J

更新日期：2018-12-01 00:00:00

abstract：:Pain can vary over the estrous cycle as a result of changes in estradiol concentration but the mechanism causing this variation is unclear. Because the thalamus is important in pain control, gene expression in the lateral thalamus (ventral posteromedial, ventral posterolateral, reticular thalamic nuclei) was screened ...

journal_title：Journal of cellular physiology

authors： Umorin M,Stinson C,Bellinger LL,Kramer PR

更新日期：2016-05-01 00:00:00

abstract：:Activation of T-lymphocytes by antigen, mitogenic lectins, or antibodies against the T-cell receptor complex, particularly in the presence of IL1, induces the secretion of the T-cell growth factor IL2. IL2 then has a major role in regulating the duration and magnitude of the immune response. Interaction of antigen, an...

journal_title：Journal of cellular physiology

authors： Mills GB,May C,Hill M,Gelfand EW

更新日期：1989-11-01 00:00:00

abstract：:Ornithine decarboxylase (ODC) activity usually rises to a peak a few hours after a trophic stimulus. The stimulation of ODC has been shown to depend on extracellular calcium in several in vitro eukaryotic systems. We have investigated the effect of calcium concentration on ODC activity and have found that ODC is stimu...

journal_title：Journal of cellular physiology

authors： Langdon RC,Fleckman P,McGuire J

更新日期：1984-01-01 00:00:00

abstract：:Signal transduction of fibroblast growth factor (FGF) receptors is known to involve tyrosine phosphorylation of several substrates, including Grb2, phospholipase C-gamma, and phosphatidylinositol 3-kinase, whereas the role of G-proteins in FGF receptor signaling is controversial. In the present study we investigated t...

journal_title：Journal of cellular physiology

authors： Piiper A,Stryjek-Kaminska D,Gebhardt R,Zeuzem S

更新日期：1996-04-01 00:00:00

abstract：:Many mammalian SWI/SNF complexes use Brahma-related gene 1 (Brg1) as a catalytic subunit to remodel nucleosomes for transcription regulation. In several mesenchymal cells and tissues, expression of a defective Brg1 protein negates the normal activity of the SWI/SNF complex and delays or blocks differentiation. To inve...

journal_title：Journal of cellular physiology

authors： Vradii D,Wagner S,Doan DN,Nickerson JA,Montecino M,Lian JB,Stein JL,van Wijnen AJ,Imbalzano AN,Stein GS

更新日期：2006-01-01 00:00:00

abstract：:Phospholipase A2 (PLA2) is pivotal in the rapid membrane-mediated actions of 1,25-dihydroxyvitamin D3 [1alpha,25(OH)2D3]. Microarray analysis indicated that PLA2 activating protein (PLAA) mRNA is upregulated 6-fold before rat growth plate cells exhibit 1alpha,25(OH)2D3-dependent protein kinase C (PKC) increases, sugge...

journal_title：Journal of cellular physiology

authors： Schwartz Z,Graham EJ,Wang L,Lossdörfer S,Gay I,Johnson-Pais TL,Carnes DL,Sylvia VL,Boyan BD

更新日期：2005-04-01 00:00:00

abstract：:Cell division, neurite formation and acetylcholinesterase activity were examined in a clone (NBA2) of mouse neuroblastoma cells maintained for up to 120 hours in medium with pH values between 6.6 and 8.0. Growth rate decreased as pH was reduced from 7.8 to 6.6. Generation time at pH 7.4 was 25 hours, while the rate of...

journal_title：Journal of cellular physiology

authors： Bear MP,Schneider FH

更新日期：1977-04-01 00:00:00

abstract：:Human fibroblasts derived from newborn foreskin and designated CF-3 were assayed for heat labile glucose-6-phosphate dehydrogenase (G6PD) when they had grown to confluency, as well as when they were arrested in an essentially nonmitotic state. Under both culture conditions there was an increase in heat labile G6PD (up...

journal_title：Journal of cellular physiology

authors： Duncan MR,Dell'orco RT,Guthrie PL

更新日期：1977-10-01 00:00:00

abstract：:The protein tyrosine kinase pp125FAK (focal adhesion kinase, or FAK) is expressed by a variety of cell types and has been implicated in integrin-mediated signaling events. We explored the potential functions of FAK by expressing it de novo in a cell type lacking FAK. We showed previously that cultured human macrophage...

journal_title：Journal of cellular physiology

authors： De Nichilo MO,Katz BZ,O'Connell B,Yamada KM

更新日期：1999-02-01 00:00:00

abstract：:Anoctamin1 (ANO1) encodes a Ca(2+)-activated chloride (Cl(-)) channel (CaCC) in variety tissues of many species. Whether ANO1 expresses and functions as a CaCC in cardiomyocytes remain unknown. The objective of this study is to characterize the molecular and functional expression of ANO1 in cardiac myocytes and the ro...

journal_title：Journal of cellular physiology

authors： Ye Z,Wu MM,Wang CY,Li YC,Yu CJ,Gong YF,Zhang J,Wang QS,Song BL,Yu K,Hartzell HC,Duan DD,Zhao D,Zhang ZR

更新日期：2015-02-01 00:00:00

abstract：:The components of unidirectional K influx and efflux have been investigated in the 3T3 cell and the SV40 transformed 3T3 cell in expontntial and stationary growth phase. Over the cell densities used for transport experiments the 3T3 cell goes from exponential growth to density dependent inhibition of growth (4 X 10(4)...

journal_title：Journal of cellular physiology

authors： Spaggiare S,Wallach MJ,Tupper JT

更新日期：1976-11-01 00:00:00

abstract：:Gene therapy using biocompatible cationic liposomes is amongst promising approaches that decreases death from cancers. Here an invasive multidrug resistant cell model has been developed by lentiviral transfection. In parallel phospholipids have been covalently conjugated to TAT, MMP2, and Herceptin. The functional lip...

journal_title：Journal of cellular physiology

authors： Gholamian Dehkordi N,Elahian F,Khosravian P,Mirzaei SA

更新日期：2019-11-01 00:00:00

abstract：:Peripheral nerve physiology and regeneration has been observed and investigated in literature but surgical applications to reconstruct and restore motor or sensory functions are still in a developmental phase. The peripheral nerve progresses slowly and incompletely compared with other tissues, it may provoke separatio...

journal_title：Journal of cellular physiology

authors： Riccio M,Marchesini A,Pugliese P,De Francesco F

更新日期：2019-04-01 00:00:00

abstract：:Maintenance of beta1 integrin-mediated cell adhesion in quiescent human mammary epithelial (HME) cells requires protein phosphatase (PP) 2A for not only dephosphorylation of beta1 integrin but also recruitment of IQGAP1 to Rac-bound beta1 integrin. However, how PP2A-dependent regulatory machinery of cell adhesion resp...

journal_title：Journal of cellular physiology

authors： Takahashi K,Suzuki K

更新日期：2006-07-01 00:00:00

abstract：:Ankylosing spondylitis (AS) is a chronic immune-mediated inflammatory disease that affects both axial and peripheral skeletons as well as soft tissues. Recent investigations offer that disease pathogenesis is ascribed to a complex interplay of genetic, environmental, and immunological factors. Until now, there is no a...

journal_title：Journal of cellular physiology

authors： Mohammadi H,Hemmatzadeh M,Babaie F,Gowhari Shabgah A,Azizi G,Hosseini F,Majidi J,Baradaran B

更新日期：2018-08-01 00:00:00

abstract：:Lesional psoriatic epidermis displays a number of phenotypic changes that are distinct from the differentiation program found in normal interfollicular epidermis. In psoriatic epidermis, keratinocytes are hyperproliferative and several differentiation-associated molecules are expressed that are absent in normal skin (...

journal_title：Journal of cellular physiology

authors： Van Ruissen F,de Jongh GJ,Zeeuwen PL,Van Erp PE,Madsen P,Schalkwijk J

更新日期：1996-08-01 00:00:00

abstract：:Nicotinamide adenine dinucleotide phosphate [NAD(P)H] oxidase complex has been shown to be involved in the process of glucose-stimulated insulin secretion (GSIS). In this study, we examined the effect of palmitic acid on superoxide production and insulin secretion by rat pancreatic islets and the mechanism involved. R...

journal_title：Journal of cellular physiology

authors： Graciano MF,Santos LR,Curi R,Carpinelli AR

更新日期：2011-04-01 00:00:00

abstract：:Multicolor immunostaining analysis is often a desirable tool in cell biology for most researchers. Nonetheless, this is not an easy task and often not affordable by many laboratories as it might require expensive instrumentation and sophisticated analysis software. Here, we describe a simple protocol for performing se...

journal_title：Journal of cellular physiology

authors： Cipressa F,Di Giorgio ML,Cenci G

更新日期：2014-06-01 00:00:00

abstract：:Growing evidence from recent studies has shown that the X-inactive specific transcript (XIST), a well-known long noncoding RNA involved in early embryonic development, is aberrantly regulated in various human cancers. However, the prognostic value of XIST in cancers remains uncharacterized. In this study, we searched ...

journal_title：Journal of cellular physiology

authors： Liu JL,Zhang WQ,Zhao M,Huang MY

更新日期：2019-05-01 00:00:00

abstract：:We have examined the transport of amino acids by the sodium-dependent "A" and "ASC" system in thymic- and splenic-derived lymphocytes from the Long-Evans rat. Lymphocytes derived from the thymus transport amino acids by both the "A" and "ASC" systems, whereas lymphocytes from the spleen transport amino acids by the "A...

journal_title：Journal of cellular physiology

authors： Wise WC

更新日期：1978-11-01 00:00:00

abstract：:Normal rat kidney [NRK] cells grown in the presence of epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) have a normal phenotype and undergo density-dependent growth inhibition, whereas in the presence of multiple growth factors, density arrest is lost and the cells become phenotypically transform...

journal_title：Journal of cellular physiology

authors： Rijksen G,Völler MC,Van Zoelen EJ

更新日期：1993-02-01 00:00:00

abstract：:Kaposi's sarcoma (KS) is the most frequent malignant lesion in patients with AIDS and is characterized by spindle cell proliferation, inflammatory cell infiltration, angiogenesis, edema, and invasiveness. KS origin is still debated. The complex aspect of this disease is probably supported by multiple concomitant patho...

journal_title：Journal of cellular physiology

authors： Montaldo F,Maffé A,Morini M,Noonan D,Giordano S,Albini A,Prat M

更新日期：2000-08-01 00:00:00

abstract：:Melanoma as the most major skin malignancy has attracted much attention, so far. Although a successful therapeutic strategy requires an accurate understanding of the precise mechanisms for the initiation and progression of the melanoma. Several types of cell death mechanisms have recently been identified along with co...

journal_title：Journal of cellular physiology

authors： Ashrafizadeh M,Mohammadinejad R,Tavakol S,Ahmadi Z,Roomiani S,Katebi M

更新日期：2019-11-01 00:00:00

abstract：:Core binding factor β (Cbfβ) is a partner protein of Runx family transcription factors with minimally characterized function in cartilage. Here we address the role of Cbfβ in cartilage by generating chondrocyte-specific Cbfβ-deficient mice (Cbfb(Δch/Δch) ) from Cbfb-floxed mice crossed with mice expressing Cre from th...

journal_title：Journal of cellular physiology

authors： Park NR,Lim KE,Han MS,Che X,Park CY,Kim JE,Taniuchi I,Bae SC,Choi JY

更新日期：2016-01-01 00:00:00

abstract：:Transforming growth factor beta type 1 (TGF-beta 1) was reacted with NHS-biotin to yield a derivative of TGF-beta 1 which was biotinylated on lysine residues. The biotinylated form of TGF-beta 1 was separated from the unreacted material by reverse phase chromatography. In three separate bioassays, the derivatized pept...

journal_title：Journal of cellular physiology

authors： Newman W,Beall LD,Bertolini DR,Cone JL

更新日期：1989-10-01 00:00:00

abstract：:Increasing evidence supports the idea that the finite proliferative life span of normal fibroblasts is a differentiation-like phenomenon. If this were correct, an ordered sequence of differential gene expression should be associated with the in vitro progression of cells from low passage to high passage (senescence). ...

journal_title：Journal of cellular physiology

authors： Choi AM,Olsen DR,Cook KG,Deamond SF,Uitto J,Bruce SA

更新日期：1992-04-01 00:00:00

abstract：:The possibility that one or both of the synthetic triamines, 1,3,6-triaminohexane and 1,4,7-triaminoheptane, could substitute for the naturally occurring polyamines in the growth of SV-3T3 cells was investigated. It was found that these triamines did lead to a restoration of growth in cells in which spermidine content...

journal_title：Journal of cellular physiology

authors： McGovern KA,Clark RS,Pegg AE

更新日期：1986-05-01 00:00:00