Abstract：
:Activation of T-lymphocytes by antigen, mitogenic lectins, or antibodies against the T-cell receptor complex, particularly in the presence of IL1, induces the secretion of the T-cell growth factor IL2. IL2 then has a major role in regulating the duration and magnitude of the immune response. Interaction of antigen, antibodies against the T-cell receptor complex, or mitogenic lectins with T-lymphocytes also induces hydrolysis of membrane phospholipids, leading to the production of diacylglycerol, an activator of the Ca2+- and phospholipid-dependent phosphotransferase, protein kinase C (PKC). Phorbol esters, potent activators of PKC, augment secretion of the T-cell growth factor, interleukin 2 (IL2). Activation of PKC may therefore serve as an important early event in the production and secretion of IL2. We have determined whether IL2 secretion can be induced in the murine cell T-lymphocyte line LBRM 331A5, where PKC is inhibited by staurosporine or sphingosine or in cells where PKC is depleted by prolonged incubation with high concentrations of phorbol esters. In cells in which PKC was either inhibited or depleted, antibodies against the T3 portion of the T-cell receptor complex and the mitogenic lectin phytohemagglutinin (PHA) still triggered IL2 secretion. In addition, the monokine IL1 augmented this IL2 secretion irrespective of whether PKC was inhibited or depleted. These data indicate that activation of PKC is not an obligatory step for IL2 secretion in LBRM 331A5 murine T-lymphocytes.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Mills GB,May C,Hill M,Gelfand EWdoi
10.1002/jcp.1041410212subject
Has Abstractpub_date
1989-11-01 00:00:00pages
310-7issue
2eissn
0021-9541issn
1097-4652journal_volume
141pub_type
杂志文章abstract：:Stromal cell-derived factor-1 (SDF-1) and CXC chemokine receptor 4 (CXCR4) have been found to be tightly correlated with the progression of prostate cancer (PC). In this study, we investigated the effects of an SDF-1α/CXCR4 inhibitor, AMD3100, on cell progression and metastasis potential of human PC cells. Human PC ce...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27831
更新日期：2019-07-01 00:00:00
abstract：:The effect of the glucocorticoids, insulin, and glucose concentration on glycogen deposition in adult rat liver parenchymal cells maintained in a chemically defined, serum-free medium has been studied. Increasing the medium concentration of glucose from 5.6 mM to 30.6 mM in the absence of hormones increased cellular g...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041100313
更新日期：1982-03-01 00:00:00
abstract：:The amino acid pools in Chinese hamster lung V79 cells were measured as a function of time during hyperthermic exposure at 40.5 degrees and 45.0 degrees C. Sixteen of the 20 protein amino acids were present in sufficient quantity to measure accurately. The total amino acid pool and all individual amino acids, except g...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041350120
更新日期：1988-04-01 00:00:00
abstract：:Using a serum-free system, we have investigated the influence of human fibronectin (HFN) and selected growth factors (GF) on the attachment and growth of normal human keratinocytes in vitro. Single-cell suspensions of keratinocytes from near-confluent primary plates, plated on 5-10 microgram/cm2 HFN, showed approximat...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041120207
更新日期：1982-08-01 00:00:00
abstract：:The present study was designed to investigate the mechanism of myeloid differentiation protein 2 (MD2) on intestinal mucosa destruction in mice with chronic colitis. Briefly, a chronic colitis mouse model was established by the administration of dextran sulfate sodium (DSS) in transgenic mice of MD2 overexpression (Tr...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28711
更新日期：2019-11-01 00:00:00
abstract：:The molecular and cellular mechanisms underlying vascular remodeling are currently investigated by experimental strategies which aim to mimic the complex environmental conditions found in vivo. Some of them focus on the tubulogenic activity of dispersed endothelial cell populations, while others evaluate vascular spro...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25468
更新日期：2017-02-01 00:00:00
abstract：:In quiescent human fibroblasts stimulated to proliferate by fresh medium plus 15% serum, no changes were seen in the incorporation of 3H tryptophan into the protein of nuclear ribonucleoprotein during the first three hours following re-feeding. This was in contrast to non-histone chromosomal proteins where the incorpo...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040920115
更新日期：1977-07-01 00:00:00
abstract：:Breast cancer stem cells (BCSCs) are competent to initiate tumor formation and growth and refractory to conventional therapies. Consequently BCSCs are implicated in tumor recurrence. Many signaling cascades associated with BCSCs are critical for epithelial-to-mesenchymal transition (EMT). We developed a model system t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29625
更新日期：2020-10-01 00:00:00
abstract：:Intervertebral disc (IVD) degeneration is the major cause of back pain. Notch signaling is activated in annulus fibrosus (AF) and nucleus pulposus (NP) tissues of degenerated IVDs, and induced by IL1-β and TNF-α in NP cells. However, the role of Notch activatin in the pathogenesis of IVD degeneration is largely unknow...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26385
更新日期：2018-07-01 00:00:00
abstract：:Laminin is a basement membrane-specific glycoprotein that promotes cell adhesion, proliferation, differentiation, and tumor cell migration. Synthetic peptides from the amino acid sequence deduced from a cDNA clone of the B1 chain of laminin were tested for their ability to promote the migration of B16F10 melanoma cell...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041340216
更新日期：1988-02-01 00:00:00
abstract：:Human dental pulp stem cells (hDPSCs) show significant potential for exploitation in novel regeneration strategies, although lack of understanding of their responses to bacterial challenge constrains their application. The present study aimed to investigate whether lipopolysaccharide (LPS), the major pathogenic factor...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24732
更新日期：2015-03-01 00:00:00
abstract：:Diabetic retinopathy (DR) is the most common ocular complication caused by diabetes mellitus and is the main cause of visual impairment in working-age people. Reactive gliosis and pro-inflammatory cytokine production by Müller cells contribute to the progression of DR. Melatonin is a strong anti-inflammatory hormone, ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29716
更新日期：2020-11-01 00:00:00
abstract：:Cyclosporine A (CyA) is a powerful nonsteroidal immunosuppressive agent used to prevent graft rejection of organ and bone marrow transplants. A major side effect observed can be attributed to the fibroblast and its functions: proliferation of fibroblasts and formation of fibrotic tissue in the gingiva (fibrous hyperpl...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041520222
更新日期：1992-08-01 00:00:00
abstract：:This review addresses peptide regions of human tumor liberated protein (TLP) complexes with antigenic activity. The review also deals with antibodies reacting with such proteins, to be used for diagnostic and clinical purposes. We identified peptide sequences of the TLP 100 kDa protein. Lung cancer cells were found po...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21851
更新日期：2009-10-01 00:00:00
abstract：:Although prostate carcinoma is an aggressive cancer preferentially metastasizing to the bones, many prostate tumors remain localized and confined to the prostate indefinitely. Prediction of the behavior of anatomically localized and moderately differentiated prostate tumors remains difficult because of lack of prognos...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20709
更新日期：2006-10-01 00:00:00
abstract：:Bone morphogenetic proteins (BMPs) play pivotal roles in bone and cartilage growth and repair. Through phenotypes of short-ear (se) mice, which have BMP-5 mutations, a role for BMP-5 in some specific aspects of skeletogenesis and cartilage growth is known. This report examines BMP-5 expression in the growth plate and ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21164
更新日期：2008-01-01 00:00:00
abstract：:To construct a long noncoding RNA (lncRNA)-microRNA (miRNA)-messenger RNA (mRNA) regulatory network related to epithelial ovarian cancer (EOC) cisplatin-resistant, differentially expressed genes (DEGs), differentially expressed lncRNAs (DELs), and differentially expressed miRNAs (DEMs) between MDAH and TOV-112D cells ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28587
更新日期：2019-11-01 00:00:00
abstract：:Abnormal glucose handling in the proximal tubule may play an important role in the development of diabetic nephropathy. Thus, the present study was designed to examine the effect of high glucose on alpha-methyl-D-glucopyranoside (alpha-MG) uptake and its signaling pathways in the primary cultured rabbit renal proximal...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(200006)183:3<355::AID-JCP8
更新日期：2000-06-01 00:00:00
abstract：:We have previously shown that retinoic acid (RA) has protective effects on high glucose (HG)-induced cardiomyocyte apoptosis. To further elucidate the molecular mechanisms of RA effects, we determined the interaction between nuclear factor (NF)-κB and RA signaling. HG induced a sustained phosphorylation of IKK/IκBα an...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24142
更新日期：2013-02-01 00:00:00
abstract：:Cdk9, a member of the cyclin-dependent kinase family, is the catalytic subunit of P-TEFb, a protein kinase complex that stimulates transcriptional elongation. Cdk9, complexed with its regulatory partner cyclin T1, serves as the cellular mediator of the transactivation function of the HIV Tat protein. There are two kno...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20224
更新日期：2005-04-01 00:00:00
abstract：:Liver fibrosis is a reversible wound-healing response that occurs after liver injury. NADPH oxidases (NOXs) and reactive oxygen species (ROS) which are expressed in hepatocytes (HCs), hepatic stellate cells (HSCs), and Kupffer cells (KCs) play an important role in the development of hepatic fibrosis. In in vitro studi...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26541
更新日期：2018-10-01 00:00:00
abstract：:Epimorphin/syntaxin 2 is a high conserved and very abundant protein involved in epithelial morphogenesis in various organs. We have shown recently that epimorphin (EPM), a protein exclusively expressed on the surface of hepatic stellate cells and myofibroblasts of the liver, induces bile duct formation of hepatic stem...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22625
更新日期：2011-11-01 00:00:00
abstract：:Resistance to conventional chemotherapy remains a major cause of cancer relapse and cancer-related deaths. Therefore, there is an urgent need to overcome resistance barriers. To improve cancer treatment approaches, it is critical to elucidate the basic mechanisms underlying drug resistance. Increasingly, the mechanism...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.29640
更新日期：2020-10-01 00:00:00
abstract：:Hyperinsulinemia is a likely cause of the increased cancer incidence and mortality in diabetic patients, but its role is difficult to define in vivo. Previous in vitro studies testing the mitogenic potential of insulin and its analogs provided incomplete and sometimes contradictory results. To better evaluate cancer c...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24635
更新日期：2014-11-01 00:00:00
abstract：:Chemotherapy resistance is the major issue of choriocarcinoma. Apoptosis always is the ultimate outcome of chemotherapeutic drugs, which considered one of the reasons of resistance. We investigated the role of STAT3/NFIL3 signaling-inhibited apoptosis in chemotherapy resistance and whether Raddeanin A (RA) could be a ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26362
更新日期：2018-07-01 00:00:00
abstract：:Several hydrolase activities characteristic of the apical brush border membrane of renal proximal tubule, leucine aminopeptidase, gamma-glutamyl transpeptidase, alkaline phosphatase, maltase, and trehalase, were identified in cultures of the LLC-PK1 kidney epithelial cell line. A coordinate increase in activities of t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041210109
更新日期：1984-10-01 00:00:00
abstract：:Fibroblastic and epithelioid clones have been isolated from the normal rat kidney line, NRK. These clones were studied for their ability to bind epidermal growth factor (EGF), susceptibility to transformation by mouse sarcoma virus (MSV), and alteration in EGF binding upon sarcoma virus transformation. The epithelioid...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040940311
更新日期：1978-03-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is one of the most common malignancies and is a serious threat to people's health worldwide. The prognosis of advanced HCC is dim if left untreated. In the clinic, the treatment options for advanced HCC include surgery, radiotherapy, transcatheter arterial chemoembolization, and so forth...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.28048
更新日期：2019-08-01 00:00:00
abstract：:A method, based on the differing capacities of cells to adhere to a column of polyester fibres, has been described for separating human bone marrow cells into a nonadherent and an adherent fraction. The effect of this cell separation procedure on colony formation by erythroid progenitor cells was investigated. In cont...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040910108
更新日期：1977-04-01 00:00:00
abstract：:The relationship of cell surface changes to proliferative decline of human diploid fibroblasts was investigated using the concanavalin A-mediated red blood cell adsorption assay. The amount of the red blood cells adsorbed to human diploid fibroblasts via concanavalin A increased continuously from the early phases of c...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041000219
更新日期：1979-08-01 00:00:00
