Abstract:
:Increasing evidence supports the idea that the finite proliferative life span of normal fibroblasts is a differentiation-like phenomenon. If this were correct, an ordered sequence of differential gene expression should be associated with the in vitro progression of cells from low passage to high passage (senescence). To define the pattern of expression of fibroblast differentiation-associated genes during this in vitro progression, we have determined the temporal pattern of expression of extracellular matrix (ECM) genes in Syrian hamster dermal fibroblasts as a function of passage level and percentage of proliferative life span in vitro. Steady-state mRNA levels were determined by Northern and dot blot analyses of total cellular RNA hybridized with cDNA probes specific for fibronectin, procollagen alpha 1III, and procollagen alpha 1I. Cells were analyzed at 24 hr postconfluence to minimize the presence of actively proliferating cells, and because maximal levels of fibronectin, alpha 1III, and alpha 1I mRNAs were observed 24 hr postconfluence. Unique, multiphasic patterns of expression of each of these ECM components were observed as the cells progressed from low passage to high passage. As the cells reached midhigh passage, fibronectin mRNA levels increased. This midpassage increase in fibronectin was followed by an increase in the level of alpha 1III mRNA as the cells reached the end of their in vitro proliferative life span, and then alpha 1I when the cells entered the postmitotic senescent phase, at which time the level of fibronectin mRNA also declined. A similar overlapping cascade pattern of up-regulation of these genes is seen during development and wound repair. This suggests that as cultured fibroblasts reach the end of their proliferative life span, they reinitiate a gene expression program used in tissue development and repair.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Choi AM,Olsen DR,Cook KG,Deamond SF,Uitto J,Bruce SAdoi
10.1002/jcp.1041510119keywords:
subject
Has Abstractpub_date
1992-04-01 00:00:00pages
147-55issue
1eissn
0021-9541issn
1097-4652journal_volume
151pub_type
杂志文章abstract::Respiratory tract epithelia are one type of tissue targeted by vitamin A. In this study the effects of vitamin A and its analogs (retinoids) on human bronchial epithelial (HBE) cells have been investigated in a serum-free hormone-supplemented medium. This serum-free medium, which was developed for the long-term cultiv...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041270110
更新日期:1986-04-01 00:00:00
abstract::NIH-3T3 cells transformed by the EJ-ras oncogene display reduced platelet-derived growth factor (PDGF)-stimulated phospholipase C activity as measured by inositol 1,4,5-triphosphate (IP3) synthesis and Ca2+ mobilization. The lack of PDGF-stimulated Ca2+ mobilization in EJ-ras transformed cells is not due to a loss of ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390216
更新日期:1989-05-01 00:00:00
abstract::Adsorption of Sendai virus at high multiplicity (500-1,000 HAU/10(6) cells) to HeLa cells grown in monolayers causes immediate changes in the ion barrier of the cell membrane, as well as changes in the morphology of the virus-treated cells. Within minutes of adsorption the cells begin to lose potassium and an extensiv...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040950212
更新日期:1978-05-01 00:00:00
abstract::Colon cancer remains one of the lethal malignancies in the world. Aberrant activation of canonical Wnt/β-catenin signaling pathway has been observed in colon cancer. In contrast, the non-canonical Wnt signaling functions remain obscure. Wnt5a is a representative non-canonical Wnt ligand which has gained extensive atte...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24566
更新日期:2014-12-01 00:00:00
abstract::Vitamin C is an essential micronutrient for the development of male germ cells. In the gonad, the germ cells are isolated from the systemic circulation by the blood-testis barrier, which consists of a basal layer of Sertoli cells that communicate through an extensive array of tight junction complexes. To study the beh...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21545
更新日期:2008-12-01 00:00:00
abstract::Reactive oxygen species (ROS) function as signaling molecules mainly by reversible oxidation of redox-sensitive target proteins. ROS can be produced in response to integrin ligation and growth factor stimulation through Rac1 and its effector protein NADPH oxidase. One of the central roles of Rac1-NADPH oxidase is acti...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22563
更新日期:2011-09-01 00:00:00
abstract::Cancer incidences are growing and cause millions of deaths worldwide. Cancer therapy is one of the most important challenges in medicine. Improving therapeutic outcomes from cancer therapy is necessary for increasing patients' survival and quality of life. Adjuvant therapy using various types of antibodies or immunomo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28122
更新日期:2019-08-01 00:00:00
abstract::C1q/tumor necrosis factor-related protein-6 (CTRP6) is a newly identified adipokine involved in diverse biological processes. However, its role in salivary glands remains unknown. Here, we demonstrated that CTRP6 was mainly distributed in the nuclei, apicolateral membranes, and cytoplasm of human submandibular glands ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30262
更新日期:2021-01-05 00:00:00
abstract::Neuropathological and clinical evidence indicates that the clinical expression of Alzheimer's disease (AD) occurs as neuropathology exceeds the brain reserve capacity. The brain or cognitive reserve (BCR) hypothesis states that high premorbid intelligence, education, and an active and stimulating lifestyle provide res...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28308
更新日期:2019-02-19 00:00:00
abstract::Identification of cellular proteins, in addition to already known transcription factors such as NF-κB, Sp1, C-EBPβ, NFAT, ATF/CREB, and LEF-1, which interact with the HIV-1 LTR, is critical in understanding the mechanism of HIV-1 replication in monocytes/macrophages. Our studies demonstrate upregulation of pyruvate ki...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25445
更新日期:2017-03-01 00:00:00
abstract::[3H]Ouabain specifically bound at sublethal concentrations to Na,K-ATPase on the surface of HeLa cells is taken up (internalized) by the cells at a rate of three membrane equivalents of labeled sites per generation. Immediately following a pulse label with the glycoside, codistribution of radioactivity with the surfac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041100114
更新日期:1982-01-01 00:00:00
abstract::Polyploidy has been linked to tumorigenicity mainly due to the chromosomal aberrations. Elevated reactive oxygen species (ROS) generation, on the other hand, has also been associated with oncogenic transformation in most cancer cells. However, a possible link between ploidy and ROS is largely unexplored. Here we have ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22793
更新日期:2012-02-01 00:00:00
abstract::Suppression of PKC activity can selectively induce apoptosis in cells expressing a constitutively activated p21Ras protein. We demonstrate that continued expression of p21Ras activity is required in PKC-mediated apoptosis because farnesyltransferase inhibitors abrogated the loss of viability in p21Ras-transformed cell...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10409
更新日期:2004-02-01 00:00:00
abstract::Several proteases and their specific inhibitors modulate the interdependent processes of cell migration and matrix proteolysis as part of the global program of trauma repair. Expression of plasminogen activator inhibitor type-1 (PAI-1), a serine protease inhibitor (SERPIN) important in the control of barrier proteolys...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20016
更新日期:2004-08-01 00:00:00
abstract::Resistin, secreted by macrophages in tumor microenvironment, has never been investigated in pancreatic cancer models, despite a vibrant tumor microenvironment around pancreatic tumors. We evaluated serum resistin levels in healthy individuals versus pancreatic cancer patients representing different tumor grades. In vi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27631
更新日期:2019-06-01 00:00:00
abstract::Cancer cells acquire dysregulated gene expression to establish specific transcriptional dependencies and their underlying mechanisms that are ultimately responsible for this addictions have not been fully elucidated. Glucose-regulated protein 78 (GRP78) is a stress-inducible, multifunctional, prosurvival, endoplasmic ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30030
更新日期:2020-08-31 00:00:00
abstract::Restitution is a crucial event during the healing of superficial injury of the gastric mucosa involving epithelial cell sheet movement into the damaged area. We demonstrated that growth factors promote the restitution of human gastric epithelial cells. However, the intracellular signaling pathways that transmit extrac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21239
更新日期:2008-02-01 00:00:00
abstract::Ebp1, an ErbB-3 binding protein, inhibits the proliferation and induces the differentiation of human breast cancer cells. The mechanisms of these effects are unknown. Rb, the product of the retinoblastoma gene, is an important modulator of cell cycle progression and cellular differentiation. We report that Rb is a bin...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1075
更新日期:2001-05-01 00:00:00
abstract::Cadmium (Cd) is a toxic metal with multiple effects on cell signaling and cell death. We studied the effects of Cd(2+) on quiescent mouse mesangial cells in serum-free conditions. Cadmium induces cell death over 6 h through annexin V+ states without or with causing uptake of propidium iodide, termed apoptotic and apop...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21499
更新日期:2008-11-01 00:00:00
abstract::The bone marrow landscape consists of specialized and stem/progenitor cells, which coordinate important tissue-related and systemic physiological features. Within the marrow cavity, stem/progenitor and differentiated hematopoietic and skeletal cells congregate into dynamic functional assemblies throughout specific ana...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30296
更新日期:2021-01-25 00:00:00
abstract::C6 glioma cells possess beta adrenergic receptors coupled with adenylate cyclase which can be irreversibly blocked by bromoacetylaminomethylpindolol (Br-AAM-pindolol), a beta adrenergic antagonist. With 1 microM Br-AAM-pindolol, more than 80% of beta adrenergic receptors, labeled by (3H)-dihydroalprenolol [3H)-DHA), w...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041210318
更新日期:1984-12-01 00:00:00
abstract::The 94-kDa glucose-regulated protein (GRP94) is a glycoprotein in the endoplasmic reticulum (ER). It has been characterized as a Ca2+-binding protein and a molecular chaperone. In this report we show that highly purified GRP94 exhibits an active Mg2+-dependent serine kinase activity (termed 94-kinase). The 94-kinase c...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199702)170:2<115::AID-JCP3
更新日期:1997-02-01 00:00:00
abstract::The expression of laminin, a major constituent of endometrial cell basement membranes, is increased during differentiation of human endometrial stromal cells (decidualization). To determine whether laminin plays a role in decidualization, we studied the effects of laminin substrate on the synthesis and release of prol...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041630105
更新日期:1995-04-01 00:00:00
abstract::The actions of prostaglandins (PG) on cAMP in dispersed chief cells from guinea pig stomach were examined and compared to the actions of these agents on pepsinogen secretion. Maximal concentrations of A, B, or E prostaglandins caused a 2-5-fold increase in pepsinogen secretion and cellular cAMP. The relative order of ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041320119
更新日期:1987-07-01 00:00:00
abstract::Human fibroblasts derived from newborn foreskin and designated CF-3 were assayed for heat labile glucose-6-phosphate dehydrogenase (G6PD) when they had grown to confluency, as well as when they were arrested in an essentially nonmitotic state. Under both culture conditions there was an increase in heat labile G6PD (up...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040930108
更新日期:1977-10-01 00:00:00
abstract::Toll-like receptors (TLRs) are usually expressed on immune cells such as macrophages, dendritic cells, mast cells, as well as on eosinophils and some epithelial cells. They play a central role in the recognition of harmful molecules that belong to invading microorganisms or internal damaged tissues, which lead to infl...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28879
更新日期:2019-12-01 00:00:00
abstract::The polyamine analogue N1,N12bis(ethyl)spermine (BESpm) is a potent inhibitor of cell proliferation and is representative of a class of agents currently in clinical trials. Previous studies have demonstrated that BESpm treatment can produce a decrease in the mRNA levels of the protooncogene c-myc resulting from decrea...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199803)174:3<380::AID-JCP1
更新日期:1998-03-01 00:00:00
abstract::New compounds with promising antidiabetic activity were synthesized. For the first time, a portion of the glibenclamide molecule was bound to a part of the core structure of thiazolidinedione to evaluate insulin secretagogue activity. Following studies in our laboratory, 4-{2-[2-(3,4-dichlorophenyl)-4-oxo-1,3-thiazoli...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26746
更新日期:2018-01-01 00:00:00
abstract::Differentiation commitment events are essential for the initiation of hemopoiesis and, in one form or another, occur continuously during adult hemopoiesis. The most studied type of differentiation commitment decision a hemopoietic cell can make involves the alternative choice of self-renewal versus the formation of pr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-4652(199711)173:2<131::AID-JCP9
更新日期:1997-11-01 00:00:00
abstract::Integrin α2β1 plays an important role in cellular migration and metastasis processes associated with prostate cancer. The aim of this study was to assess whether selective inhibition of integrin α2β1 is an effective strategy to target metastatic prostate cancer cells. In this regard, we examined the effects of the inh...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30202
更新日期:2020-12-10 00:00:00