Abstract:
:The expression of laminin, a major constituent of endometrial cell basement membranes, is increased during differentiation of human endometrial stromal cells (decidualization). To determine whether laminin plays a role in decidualization, we studied the effects of laminin substrate on the synthesis and release of prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1), two major secretory proteins of decidualized stromal cells. Endometrial stromal cells were plated on laminin as well as several other extracellular matrix (ECM) proteins (types 1 and IV collagen or fibronectin) and on plastic, and cultured in media containing medroxyprogesterone acetate (MPA) and estradiol. Cells cultured on plastic or ECM proteins displayed similar morphological changes indicative of decidualization. However, the release of PRL and IGFBP-1 from cells cultured on plastic and ECM proteins (types 1 and IV collagen and fibronection) was approximately 2.1-fold and 2.8-fold greater respectively, than from cells cultured on laminin. The decrease in PRL and IGFBP-1 expression in cells cultured on laminin was not due to differences in initial cell attachment efficiency or final DNA content. In addition, laminin had no effect on the content of laminin protein or fibronectin mRNA levels, indicating that the effects of laminin on PRL and IGFBP-1 were specific. PGE2 stimulated the release of PRL and IGFBP-1 from cells cultured on laminin to levels comparable to those from cells cultured on plastic or other ECM proteins. This indicates that the decrease in PRL and IGFBP-1 release by laminin was not due to a generalized unresponsiveness. In contrast to the effects of laminin during decidualization, PRL expression was not altered by laminin in terminally differentiated decidual cells isolated at term. Our results support a role for laminin in selectively regulating PRL and IGFBP-1 gene expression during in vitro decidualization of human endometrial stromal cells.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Brar AK,Frank GR,Richards RG,Meyer AJ,Kessler CA,Cedars MI,Klein DJ,Handwerger Sdoi
10.1002/jcp.1041630105subject
Has Abstractpub_date
1995-04-01 00:00:00pages
30-7issue
1eissn
0021-9541issn
1097-4652journal_volume
163pub_type
杂志文章abstract::Preincubation of human neutrophils with phorbol esters or soluble diglycerides enhances subsequent f-Met-Leu-Phe (fMLP)-stimulated arachidonate mobilization and leukotriene B4 (LTB4) synthesis. We have recently reported that 1,3-dioctanoylglycerol (1,3-diC8) is equipotent with 1,2-sn-dioctanoylglycerol (1,2-diC8) as p...
journal_title:Journal of cellular physiology
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abstract::Schizophrenia (SZ) is characterized by a high morbidity and disability rate and has gradually increased in rate and caused much burden. However, the pathogenesis of SZ is elusive and may include changes in the biological molecules in exosomes. In this study, we first compared the alterations of plasma exosomal circula...
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journal_title:Journal of cellular physiology
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更新日期:1986-06-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:1983-10-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:1994-07-01 00:00:00
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390106
更新日期:1989-04-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2006-02-01 00:00:00
abstract::The effect of changes in muscle length on post-tetanic isometric twitch tension potentiation and myosin P-light chain phosphorylation was studied at 23 degrees C in the mouse extensor digitorum longus muscle. The length-tension relationship was determined for the same muscles after a 30 min period of quiescence and be...
journal_title:Journal of cellular physiology
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doi:10.1002/jcp.1041430209
更新日期:1990-05-01 00:00:00
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doi:10.1002/jcp.1041650309
更新日期:1995-12-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2010-03-01 00:00:00
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doi:10.1002/jcp.24798
更新日期:2015-08-01 00:00:00
abstract::When 3T6 cells undergo a serum-induced transition from resting to growing state, the number of ribosomes and the amounts of mRNA increase as the cells prepare for DNA synthesis. We have examined the effect of preventing ribosome synthesis during this transition. When resting cells are stimulated to grow in the presenc...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040870202
更新日期:1975-12-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:1983-09-01 00:00:00
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journal_title:Journal of cellular physiology
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doi:10.1002/jcp.1041040311
更新日期:1980-09-01 00:00:00
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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更新日期:2009-09-01 00:00:00
abstract::Laminin is a basement membrane-specific glycoprotein that promotes cell adhesion, proliferation, differentiation, and tumor cell migration. Synthetic peptides from the amino acid sequence deduced from a cDNA clone of the B1 chain of laminin were tested for their ability to promote the migration of B16F10 melanoma cell...
journal_title:Journal of cellular physiology
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更新日期:1988-02-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:1993-03-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2006-11-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:1999-11-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2008-11-01 00:00:00