Abstract:
:We have previously shown that knockout of fibroblast growth factor-2 (FGF-2) and potential compensatory effects of other growth factors result in amelioration of disease symptoms in a transgenic mouse model of amyotrophic lateral sclerosis (ALS). ALS is a rapidly progressive neurological disorder leading to degeneration of cortical, brain stem, and spinal motor neurons followed by subsequent denervation and muscle wasting. Mutations in the superoxide dismutase 1 (SOD1) gene are responsible for approximately 20% of familial ALS cases and SOD1 mutant mice still are among the models best mimicking clinical and neuropathological characteristics of ALS. The aim of the present study was a thorough characterization of FGF-2 and other growth factors and signaling effectors in vivo in the SOD1G93A mouse model. We observed tissue-specific opposing gene regulation of FGF-2 and overall dysregulation of other growth factors, which in the gastrocnemius muscle was associated with reduced downstream extracellular-signal-regulated kinases (ERK) and protein kinase B (AKT) activation. To further investigate whether the effects of FGF-2 on motor neuron death are mediated by glial cells, astrocytes lacking FGF-2 were cocultured together with mutant SOD1 G93A motor neurons. FGF-2 had an impact on motor neuron maturation indicating that astrocytic FGF-2 affects motor neurons at a developmental stage. Moreover, neuronal gene expression patterns showed FGF-2- and SOD1 G93A -dependent changes in ciliary neurotrophic factor, glial-cell-line-derived neurotrophic factor, and ERK2, implying a potential involvement in ALS pathogenesis before the onset of clinical symptoms.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Kefalakes E,Böselt S,Sarikidi A,Ettcheto M,Bursch F,Naujock M,Stanslowsky N,Schmuck M,Barenys M,Wegner F,Grothe C,Petri Sdoi
10.1002/jcp.27498subject
Has Abstractpub_date
2019-05-01 00:00:00pages
7395-7410issue
5eissn
0021-9541issn
1097-4652journal_volume
234pub_type
杂志文章abstract::Increased vascular resistance in the fetoplacental circulation is a characteristic of preeclampsia. However, the potential molecular mechanisms of this condition remain obscure. The current study aimed to determine the direct effect of the peptide antigen corresponding to the second extracellular loop of the angiotens...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24113
更新日期:2013-01-01 00:00:00
abstract::Previous studies have indicated that intracellular Ca2+ is involved in fetal bovine serum (FBS)- or growth factor (GF)-stimulated Na+ influx in human foreskin fibroblasts (HSWP). In the present study, 45Ca2+ efflux from serum-deprived HSWP cells was measured in response to 10% FBS or GF [lys-bradykinin, vasopressin, e...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041170105
更新日期:1983-10-01 00:00:00
abstract::Recent studies have indicated that endoplasmic reticulum stress, the unfolded protein response activation and altered GRP78 expression can play an important role in a variety of tumors development and progression. Very recently we reported for the first time that GRP78 is increased in endometrial tumors. However, whet...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24578
更新日期:2014-10-01 00:00:00
abstract::Respiratory tract epithelia are one type of tissue targeted by vitamin A. In this study the effects of vitamin A and its analogs (retinoids) on human bronchial epithelial (HBE) cells have been investigated in a serum-free hormone-supplemented medium. This serum-free medium, which was developed for the long-term cultiv...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041270110
更新日期:1986-04-01 00:00:00
abstract::Extracellular ATP and other nucleotides induce autocrine and/or paracrine purinergic signalling via activation of the P2 receptors on the cell surface, which represents one of the most common signalling mechanisms. Mesenchymal stem cells (MSC) are a type of multipotent adult stem cells that have many promising applica...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.25484
更新日期:2017-02-01 00:00:00
abstract::Established RAW264.7 cell lines for osteoclastic differentiation has been widely engaged in bone homeostasis research, however, the efficacy of RANKL independently stimulating has rarely been defined, because protocols were usually developed and modified by various laboratories. Otherwise, problematic issues are also ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27852
更新日期:2019-07-01 00:00:00
abstract::The trabecular meshwork (TM) is a specialized tissue located at the chamber angle of the eye next to the cornea. This tissue is believed to be responsible for regulation of the aqueous humor outflow and control of the intraocular pressure (IOP). Alterations in functions of the TM may lead to IOP elevation and developm...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10032
更新日期:2002-01-01 00:00:00
abstract::Identification of cellular proteins, in addition to already known transcription factors such as NF-κB, Sp1, C-EBPβ, NFAT, ATF/CREB, and LEF-1, which interact with the HIV-1 LTR, is critical in understanding the mechanism of HIV-1 replication in monocytes/macrophages. Our studies demonstrate upregulation of pyruvate ki...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25445
更新日期:2017-03-01 00:00:00
abstract::Mouse fetuses at 15th and 18th day of gestation, and newborns aging 0, 5, 10, and 15 days were injected i.p. with 131 I-albumin (RISA). The degree of incorporation by liver and the intraparticulate hydrolysis of RISA in 27,000g x 10 min. particles increased after birth. By this time, changes in subcellular distributio...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041090211
更新日期:1981-11-01 00:00:00
abstract::Integrin α2β1 plays an important role in cellular migration and metastasis processes associated with prostate cancer. The aim of this study was to assess whether selective inhibition of integrin α2β1 is an effective strategy to target metastatic prostate cancer cells. In this regard, we examined the effects of the inh...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30202
更新日期:2020-12-10 00:00:00
abstract::Male breast cancer (MBC) is an uncommon malignancy. We have previously reported that the expression of the Hippo transducers TAZ/YAP and their target CTGF was associated with inferior survival in MBC patients. Preclinical evidence demonstrated that Axl is a transcriptional target of TAZ/YAP. Thus, we herein assessed A...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25745
更新日期:2017-08-01 00:00:00
abstract::We have examined the ability of epidermal growth factor (EGF) to regulate prostacyclin production by cultured A10 smooth muscle cells. EGF by itself had no effect on prostacyclin production, but it augmented the response to arg8-vasopressin. An AGF stimulation of prostacyclin production was also observed in the presen...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390311
更新日期:1989-06-01 00:00:00
abstract::Construction of artificial organs and tissues by tissue engineering is strongly dependent on the availability of viable cells. For that reason, the viability and the physiological status of cells kept in culture must be evaluated before the cells can be used for clinical purposes. In this work, we determined the viabi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20976
更新日期:2007-06-01 00:00:00
abstract::Glycochenodeoxycholate (GCDA) is closely associated with carcinogenesis and chemoresistance of hepatocellular carcinoma (HCC). Signal transducer and activator of transcription 3 (STAT3), a transcription factor, is involved in various human tumors. Whether GCDA induces chemoresistance through STAT3 and the mechanism of...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29159
更新日期:2020-03-01 00:00:00
abstract::We have previously demonstrated that the rate of fluid shear stress (ΔSS) can manipulate the fate of mesenchymal stem cells (MSCs) to osteogenic or chondrogenic cells. However, whether ΔSS is comparable to other two means of induction medium and substrate stiffness that have been proven to be potent in differentiation...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28296
更新日期:2019-02-19 00:00:00
abstract::Effects of interstitial fluid flow on osteoblasts were investigated. Intracellular cyclic adenosine monophosphate (cAMP) levels were monitored in cultured osteoblasts subjected to shear rates ranging from 10 to 3,500 sec-1. Cyclic AMP levels were significantly increased at all shear rates from 1 pmole/mg protein to 10...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041430113
更新日期:1990-04-01 00:00:00
abstract::Balb/C3T3 cells entered the quiescent Go state following serum deprivation. On addition of fresh serum, more than 95% of the culture resumed growth, but with asynchronous kinetics. If hydroxyurea were added just before the first cells reached S phase, at at least 90% of the cells accumulated at the Gl/S border over th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040900219
更新日期:1977-02-01 00:00:00
abstract::Transformation of Sprague-Dawley rat embryo (RE) cells and a cloned Fischer rat embryo cell line (CREF) with wild-type (Ad5) or a temperature-sensitive DNA-minus mutant (H5ts125) of type 5 adenovirus results in a reduction in binding of epidermal growth factor (EGF) to cell surface receptors. A reduction in EGF bindin...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041140315
更新日期:1983-03-01 00:00:00
abstract::NGF appears to be involved in spermatogenesis. However, mice lacking NGF or TrkA genes do not survive more than a few days whereas p75(NTR) knockout mice are viable and fertile. Therefore, we addressed the effect of betaNGF on spermatogenesis by using the systems of rat germ cell culture we established previously. bet...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20805
更新日期:2007-01-01 00:00:00
abstract::Myocardium infarction is one of the most deathly cardiovascular diseases. It is characterized by myocardium ischemia as a result of nutrients depletion and hypoxia. The cell can respond to this injury by autophagy or apoptosis, which determines the evolution and possible recovery of the myocardium infarction. Lipids p...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25344
更新日期:2016-10-01 00:00:00
abstract::We have used HeLa cells without mitochondrial DNA (rho0-cells) and transient rho0-phenocopies, obtained from wild-type cells by short-term treatment with ethidium bromide, to analyze how the absence of a functional mitochondrial respiratory chain slows down proliferation. We ruled out an energetic problem (ATP/ADP con...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20711
更新日期:2006-10-01 00:00:00
abstract::The process of induction of human malignant T-lymphoblastic cell line MOLT-3 by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) was examined. It was found that the induction process by TPA, which included increase in cells with receptors to sheep red blood cells (E--rosette positive--E+) and decrease in t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041090120
更新日期:1981-10-01 00:00:00
abstract::Pancreatic cancer is a common malignant digestive disease. Epidemiological and clinical studies have demonstrated that pancreatic cancer is closely related to diabetes mellitus. Diabetic patients are more likely to develop pancreatic cancer, which is linked with poor outcomes. Pancreatic cancer is complicated with abn...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.29605
更新日期:2020-09-01 00:00:00
abstract::The smooth muscle cell population in major arteries of humans and experimental animals is heterogeneous with regard to cellular DNA content. A proportion of cells has polyploid DNA content and this proportion increases with normal aging and with hypertension. We have isolated pure populations of rat aortic smooth musc...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041250322
更新日期:1985-12-01 00:00:00
abstract::Helicobacter pylori lives in the stomach lumen adhering and specifically interacting with gastric epithelial cells. H. pylori infection can cause a broad range of diseases. Although most infected individuals only develop a chronic inflammation of the stomach, some patients progress to chronic gastritis, duodenal ulcer...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.20022
更新日期:2004-09-01 00:00:00
abstract::The physiological approach suggests that an environment associating the mesenchymal stromal cells (MSC) and low O(2) concentration would be most favorable for the maintenance of hematopoietic stem cells (HSCs) in course of ex vivo expansion of hematopoietic grafts. To test this hypothesis, we performed a co-culture of...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.23019
更新日期:2012-06-01 00:00:00
abstract::A serum factor which enhances the growth/viability of SV40 transformed 3T3 mouse fibroblasts, but not untransformed 3T3 cells in tissue culture has been partially purified from calf serum. The purification, which involves acidification to pH 2, chromatography on Sephadex G-100 at pH 2 followed by either Sephadex G-25 ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040960203
更新日期:1978-08-01 00:00:00
abstract::Thioredoxin reductase (TrxR) is a flavoprotein that contains a C-terminal penultimate selenocysteine (Sec) and has an ability to reduce thioredoxin (Trx), which regulates the activity of NF-kappa B. To date, three TrxR isozymes, TrxR1, TrxR2, and TrxR3, have been identified. In the present study, we found that among t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10377
更新日期:2004-01-01 00:00:00
abstract::Interest in the role of the insulin-like growth factor (IGF) axis in growth control and carcinogenesis has recently been increased by the finding of elevated serum insulin-like growth factor I (IGF-I) levels in association with three of the most prevalent cancers in the United States: prostate cancer, colorectal cance...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-4652(200004)183:1<1::AID-JCP1>3
更新日期:2000-04-01 00:00:00
abstract::Indian hedgehog (Ihh) is produced by growth plate pre-hypertrophic chondrocytes, and is an important regulator of endochondral ossification. However, little is known about the regulation of Ihh in chondrocytes. We have examined the role of integrins and mitogen-activated protein (MAP) kinases in Ihh mRNA regulation in...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20208
更新日期:2005-04-01 00:00:00