CRM197 in Combination With shRNA Interference of VCAM-1 Displays Enhanced Inhibitory Effects on Human Glioblastoma Cells.

Abstract:

:CRM197 is a naturally nontoxic diphtheria toxin mutant that binds and inhibits heparin-binding epidermal growth factor-like growth factor. CRM197 serves as carrier protein for vaccine and other therapeutic agents. CRM197 also inhibits the growth, migration, invasion, and induces apoptosis in various tumors. Vascular cell adhesion molecule-1 (VCAM-1) is an important cell surface adhesion molecule associated with malignancy of gliomas. In this work, we aimed to investigate the role and mechanism of CRM197 combined with shRNA interference of VCAM-1 (shRNA-VCAM-1) on the migration, invasion, and apoptosis of glioblastoma cells. U87 and U251 human glioblastoma cells were treated with CRM197 (10 µg/ml) and shRNA interfering technology was employed to silence VCAM-1 expression. Cell viability, migration, invasiveness, and apoptosis were assessed with CCK8, Transwell and Annexin V-PE/7-AAD staining. Activation of cleaved caspase-3, 8, and 9, activity of matrix metalloproteinase-2/9 (MMP-2/9), and expression of phosphorylated Akt (p-Akt) were also checked. Results showed that CRM197 and shRNA-VCAM-1 not only significantly inhibited the cell proliferation, migration, invasion, but also promoted the apoptosis of U87 and U251 cells. Combined treatment of both displayed enhanced inhibitory effects on the malignant biological behavior of glioma cells. The activation of cleaved caspase-3, 8, 9 was promoted, activity of MMP-2 and MMP-9 and expression of p-Akt were inhibited significantly by the treatment of CRM197 and shRNA-VCAM-1 alone or in combination, indicating that the combination of CRM197 with shRNA-VCAM-1 additively inhibited the malignant behavior of human glioblastoma cells via activating caspase-3, 8, 9 as well as inhibiting MMP-2, MMP-9, and Akt pathway.

journal_name

J Cell Physiol

authors

Hu Y,Lin X,Wang P,Xue YX,Li Z,Liu LB,Yu B,Feng TD,Liu YH

doi

10.1002/jcp.24798

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

1713-28

issue

8

eissn

0021-9541

issn

1097-4652

journal_volume

230

pub_type

杂志文章
  • Changes in the levels of viral and cellular gene-transcripts in the cell cycle of SV40 transformed mouse cells.

    abstract::We have analyzed the regulation of transcription of integrated SV40 DNA and of five cellular genes during the cell cycle of two lines of SV40 transformed mouse 3T3 cells. These cells (ts SV3T3) are temperature sensitive for the expression of the transformed phenotype and at the nonpermissive temperature (39 degrees C)...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041170110

    authors: La Bella F,Brown EH,Basilico C

    更新日期:1983-10-01 00:00:00

  • In vitro evaluation of different methods of handling human liposuction aspirate and their effect on adipocytes and adipose derived stem cells.

    abstract::Nowadays, fat tissue transplantation is widely used in regenerative and reconstructive surgery. However, a shared method of lipoaspirate handling for ensuring a good quality fat transplant has not yet been established. The study was to identify a method to recover from the lipoaspirate samples the highest number of hu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24965

    authors: Palumbo P,Miconi G,Cinque B,La Torre C,Lombardi F,Zoccali G,Orsini G,Leocata P,Giuliani M,Cifone MG

    更新日期:2015-08-01 00:00:00

  • Histomorphology and innate immunity during the progression of osteoarthritis: Does synovitis affect cartilage degradation?

    abstract::Osteoarthritis (OA) is a common chronic degenerative disease that affects all joints. At present, the pathological processes and mechanisms of OA are still unclear. Innate immunity, a key player in damage to the structure of the joint and the mechanism by which the host attempts to repair OA, affects all pathological ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26011

    authors: Wang H,Wang Q,Yang M,Yang L,Wang W,Ding H,Zhang D,Xu J,Tang X,Ding H,Wang Q

    更新日期:2018-02-01 00:00:00

  • Pulsed electromagnetic fields regulate osteocyte apoptosis, RANKL/OPG expression, and its control of osteoclastogenesis depending on the presence of primary cilia.

    abstract::Growing evidence has shown that pulsed electromagnetic fields (PEMF) can modulate bone metabolism in vivo and regulate the activities of osteoblasts and osteoclasts in vitro. Osteocytes, accounting for 95% of bone cells, act as the major mechanosensors in bone for transducing external mechanical signals and producing ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27734

    authors: Wang P,Tang C,Wu J,Yang Y,Yan Z,Liu X,Shao X,Zhai M,Gao J,Liang S,Luo E,Jing D

    更新日期:2019-07-01 00:00:00

  • miR-497a-5p attenuates lipopolysaccharide-induced inflammatory injury by targeting IRAK2.

    abstract::Acute lung injury (ALI) is a severe acute inflammatory reaction of the lungs caused by a variety of factors, which can lead to a high mortality rate. MicroRNAs are a novel therapeutic molecule that play a vital role in many diseases. However, its mechanism of action in lipopolysaccharide (LPS)-induced mouse ALI is not...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28850

    authors: Guo S,Chen Y,Liu J,Yang J,Yang C,Zhang T,Jiang K,Wu Z,Shaukat A,Deng G

    更新日期:2019-12-01 00:00:00

  • Functional characterization of PKDREJ, a male germ cell-restricted polycystin.

    abstract::Polycystin-1 regulates a number of cellular processes through the formation of complexes with the polycystin-2 ion channel or with other signal transduction proteins. Polycystin-1 is expressed in many tissues but other members of this gene family are distributed in a more restricted fashion. PKDREJ expression has been...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20755

    authors: Sutton KA,Jungnickel MK,Ward CJ,Harris PC,Florman HM

    更新日期:2006-11-01 00:00:00

  • Evaluation of efficacy on RANKL induced osteoclast from RAW264.7 cells.

    abstract::Established RAW264.7 cell lines for osteoclastic differentiation has been widely engaged in bone homeostasis research, however, the efficacy of RANKL independently stimulating has rarely been defined, because protocols were usually developed and modified by various laboratories. Otherwise, problematic issues are also ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27852

    authors: Song C,Yang X,Lei Y,Zhang Z,Smith W,Yan J,Kong L

    更新日期:2019-07-01 00:00:00

  • Heparin potentiates the action of acidic fibroblast growth factor by prolonging its biological half-life.

    abstract::The mechanism(s) by which heparin influences the biological activities of acidic and basic fibroblast growth factors (aFGF and bFGF) is not completely understood. One mechanism by which heparin could alter the biological activities of aFGF and bFGF is by altering their biological half-lives. We investigated the possib...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041380202

    authors: Damon DH,Lobb RR,D'Amore PA,Wagner JA

    更新日期:1989-02-01 00:00:00

  • Fibronectin promotes the elongation of microvessels during angiogenesis in vitro.

    abstract::Fibronectin is a component of the extracellular matrix of developing microvessels whose role in angiogenesis is poorly understood. This study evaluated the effect of plasma fibronectin on angiogenesis in serum-free collagen gel culture of rat aorta. Aortic explants embedded in collagen gels generated microvascular out...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041540325

    authors: Nicosia RF,Bonanno E,Smith M

    更新日期:1993-03-01 00:00:00

  • Serum stimulation of potassium fluxes, ouabain binding, and sodium fluxes in quiescent chicken embryo fibroblasts.

    abstract::Growth-contingent alterations in potassium and sodium fluxes, ouabain binding, and potassium ion content were examined following serum stimulation of quiescent, density-inhibited chicken embryo fibroblasts. Serum stimulation resulted in very rapid 1.5- to 1.8-fold increases in ouabain-sensitive potassium influx and le...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041030222

    authors: Johnson MA,Weber MJ

    更新日期:1980-05-01 00:00:00

  • Growth-stimulating effect of catecholamines on rat aortic smooth muscle cells in culture.

    abstract::The effect of epinephrine was tested on the proliferation of rat arterial smooth muscle cells (SMC) in secondary cultures. Epinephrine added daily to the culture medium caused a striking stimulation of growth. The effect increased with time and was dose-dependent. Maximal stimulation was observed at a concentration of...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041160207

    authors: Blaes N,Boissel JP

    更新日期:1983-08-01 00:00:00

  • Calcium stimulates ornithine decarboxylase activity in cultured mammalian epithelial cells.

    abstract::Ornithine decarboxylase (ODC) activity usually rises to a peak a few hours after a trophic stimulus. The stimulation of ODC has been shown to depend on extracellular calcium in several in vitro eukaryotic systems. We have investigated the effect of calcium concentration on ODC activity and have found that ODC is stimu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041180109

    authors: Langdon RC,Fleckman P,McGuire J

    更新日期:1984-01-01 00:00:00

  • Evidences of a role for eukaryotic translation initiation factor 5A (eIF5A) in mouse embryogenesis and cell differentiation.

    abstract::Eukaryotic translation initiation factor 5A (eIF5A) has a unique character: the presence of an unusual amino acid, hypusine, which is formed by post-translational modifications. Even before the identification of hypusination in eIF5A, the correlation between hypusine formation and protein synthesis, shifting cell prol...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22229

    authors: Parreiras-e-Silva LT,Luchessi AD,Reis RI,Oliver C,Jamur MC,Ramos RG,Oliveira EB,Curi R,Costa-Neto CM

    更新日期:2010-11-01 00:00:00

  • Comparative genomics of NAD(P) biosynthesis and novel antibiotic drug targets.

    abstract::NAD(P) is an indispensable cofactor for all organisms and its biosynthetic pathways are proposed as promising novel antibiotics targets against pathogens such as Mycobacterium tuberculosis. Six NAD(P) biosynthetic pathways were reconstructed by comparative genomics: de novo pathway (Asp), de novo pathway (Try), NmR pa...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.22419

    authors: Bi J,Wang H,Xie J

    更新日期:2011-02-01 00:00:00

  • β2-Adrenoreceptor-Mediated Proliferation Inhibition of Embryonic Pluripotent Stem Cells.

    abstract::Adrenoreceptors (ARs) are widely expressed and play essential roles throughout the body. Different subtype adrenoceptors elicit distinct effects on cell proliferation, but knowledge remains scarce about the subtype-specific effects of β2-ARs on the proliferation of embryonic pluripotent stem (PS) cells that represent ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24937

    authors: Sun F,Yang XJ,Lv HY,Tang YB,An SM,Ding XP,Li WB,Teng L,Shen Y,Chen HZ,Zhu L

    更新日期:2015-11-01 00:00:00

  • New Roles of Lkb1 in Regulating Adipose Tissue Development and Thermogenesis.

    abstract::Adipose tissues regulate energy metabolism and reproduction. There are three types of adipocytes (brown, white, and beige adipocytes) in mammals. White adipocytes store energy and are closely associated with obesity and other metabolic diseases. The beige and brown adipocytes have numerous mitochondria and high levels...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.25643

    authors: Xu Z,Liu J,Shan T

    更新日期:2017-09-01 00:00:00

  • Epigallocatechin-3-gallate activates the AMP-activated protein kinase signaling pathway to reduce lipid accumulation in canine hepatocytes.

    abstract::Epigallocatechin-3-gallate (EGCG) plays a crucial role in hepatic lipid metabolism. However, the underlying regulatory mechanism of hepatic lipid metabolism by EGCG in canine is unclear. Primary canine hepatocytes were treated with EGCG (0.01, 0.1, or 1 μM) and BML-275 (an AMP-activated protein kinase [AMPK] inhibitor...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29869

    authors: Ding H,Li Y,Li W,Tao H,Liu L,Zhang C,Kong T,Feng S,Li J,Wang X,Wu J

    更新日期:2021-01-01 00:00:00

  • Contrasting effects of the protein kinase C inhibitor staurosporine on the interleukin-1 and phorbol ester activation pathways in the EL4-6.1 thymoma cell line.

    abstract::EL 4-6.1 cells, variants of the murine EL4 thymoma cell line, can be activated by interleukin 1 (IL-1) or phorbol 12-myristate-13-acetate (PMA), or PMA+IL-1 to secrete interleukin 2 (IL-2) and interleukin 4 (IL-4) and to express the IL-2 receptor (IL-2R). To compare the different activation pathways, we examined the e...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041510112

    authors: Dornand J,Bouaboula M,d'Angeac AD,Favero J,Shire D,Casellas P

    更新日期:1992-04-01 00:00:00

  • Toll-like receptors in the functional orientation of cardiac progenitor cells.

    abstract::Cardiac progenitor cells (CPCs) have the potential to differentiate into several cell lineages with the ability to restore in cardiac tissue. Multipotency and self-renewal activity are the crucial characteristics of CPCs. Also, CPCs have promising therapeutic roles in cardiac diseases such as valvular disease, thrombo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.28738

    authors: Yousefi M,Mamipour M,Sokullu SE,Ghaderi S,Amini H,Rahbarghazi R

    更新日期:2019-11-01 00:00:00

  • Synthesis of glycolytic and peroxisomal enzymes in Tetrahymena following a change in culture conditions.

    abstract::The specific activity of a peroxisomal enzyme, lactate oxidase, and of pyruvate kinase and lactate dehydrogenase, which are not peroxisomal, increased rapidly when shaken cultures of Tetrahymena were transferred to conditions of oxygen restriction and supplemented with glucose. Two other peroxisomal enzymes, catalase ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040850106

    authors: Levy MR

    更新日期:1975-02-01 00:00:00

  • Melatonin attenuates TNF-α-mediated hepatocytes damage via inhibiting mitochondrial stress and activating the Akt-Sirt3 signaling pathway.

    abstract::The role of mitochondrial dysfunction and its molecular mechanism in inflammation-induced acute liver failure (ALF) remain unknown. Despite the numerous studies performed to date, very few therapies are available for inflammation-induced ALF. Therefore, our study is aimed to explore the regulatory effects of mitochond...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28701

    authors: Song J,Lu C,Zhao W,Shao X

    更新日期:2019-11-01 00:00:00

  • Ephrin A1 promotes proliferation of bovine endometrial cells with abundant expression of proliferating cell nuclear antigen and cyclin D1 changing the cell population at each stage of the cell cycle.

    abstract::Ephrin A1 has a role in a variety of biological events, including cell proliferation, differentiation, migration, and angiogenesis. Ephrin A1 expression is abundant in trophoblasts and endometrial cells during the implantation period; however, its intracellular activities have not yet been reported in bovine endometri...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27275

    authors: Lim W,Bae H,Bazer FW,Song G

    更新日期:2019-04-01 00:00:00

  • The adhesive glycoprotein laminin is an agglutinin.

    abstract::The glycoprotein laminin appears to function in the attachment of various epithelial cells to basement membranes. We examined whether its putative cell-adhesive activity could be analyzed in a simple, one-component model system--the agglutination of erythrocytes. Laminin is a potent agglutinin of aldehyde-fixed sheep ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041140302

    authors: Kennedy DW,Rohrbach DH,Martin GR,Momoi T,Yamada KM

    更新日期:1983-03-01 00:00:00

  • Hypoxia impairs cell fusion and differentiation process in human cytotrophoblast, in vitro.

    abstract::During human pregnancy, the trophoblast develops from differentiation of cytotrophoblast cells into an endocrine active syncytiotrophoblast. In culture, isolated mononuclear cytotrophoblasts aggregate and then fuse to form a syncytium, reproducing the in vivo process. In this study, we examined the effect of low oxyge...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199608)168:2<346::AID-JCP1

    authors: Alsat E,Wyplosz P,Malassiné A,Guibourdenche J,Porquet D,Nessmann C,Evain-Brion D

    更新日期:1996-08-01 00:00:00

  • Polyamine transport regulation by calcium and calmodulin: role of Ca(2+)-ATPase.

    abstract::The study was conducted on human leukemia (K 562) cells to characterize the mechanisms implicated in the regulation of the polyamine spermidine (Spd) transport process. The antagonists of calmodulin, trifluoperazine (TFP), W-7 (N-[6-aminohexyl]-5-chloro-1-naphthelenesulfonamide), or mellitin inhibited significantly po...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041570308

    authors: Khan NA,Sezan A,Quemener V,Moulinoux JP

    更新日期:1993-12-01 00:00:00

  • AKT and MAPK signaling in KGF-treated and UVB-exposed human epidermal cells.

    abstract::Regulation of proliferation and differentiation in keratinocyte is a complex and dynamic process that involves activation of multiple signaling pathways triggered by different growth factors. Keratinocyte growth factor (KGF) is not only a potent mitogen, but differently from other growth factors, is a potent inducer o...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21056

    authors: Lotti LV,Rotolo S,Francescangeli F,Frati L,Torrisi MR,Marchese C

    更新日期:2007-09-01 00:00:00

  • Fibronectin synthesis by high glucose level mediated proliferation of mouse embryonic stem cells: Involvement of ANG II and TGF-beta1.

    abstract::The role of individual supplements necessary for the long-term self-renewal of embryonic stem (ES) cells is poorly characterized in feeder/serum-free culture systems. This study sought to characterize the relationship between the effects of glucose on ES cell proliferation and fibronectin (FN) synthesis, and to assess...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22048

    authors: Kim YH,Ryu JM,Lee YJ,Han HJ

    更新日期:2010-05-01 00:00:00

  • BRG1 mutations found in human cancer cell lines inactivate Rb-mediated cell-cycle arrest.

    abstract::Eukaryotic organisms package DNA into chromatin for compact storage in the cell nucleus. However, this process promotes transcriptional repression of genes. To overcome the transcriptional repression, chromatin remodeling complexes have evolved that alter the configuration of chromatin packaging of DNA into nucleosome...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22533

    authors: Bartlett C,Orvis TJ,Rosson GS,Weissman BE

    更新日期:2011-08-01 00:00:00

  • Susceptibility to verotoxin as a function of the cell cycle.

    abstract::Infection with Verotoxin producing Escherichia coli (VTEC) has been implicated in hemolytic uremic syndrome, the leading cause of pediatric renal failure. Verotoxin (VT) binds to globotriaosylceramide (Gal alpha 1-4Gal beta 1-4GlcCer Gb3) in susceptible cells. Gb3 is required for cytotoxicity and toxin-resistant cells...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041500324

    authors: Pudymaitis A,Lingwood CA

    更新日期:1992-03-01 00:00:00

  • Dysregulated calcium homeostasis and oxidative stress in chronic myeloid leukemia (CML) cells.

    abstract::Chronic myeloid leukemia (CML) is a hematopoietic stem cell disorder caused by the oncogenic activity of the Bcr-Abl protein, a deregulated tyrosine kinase. Calcium may act directly on cellular enzymes and in conjunction with other cellular metabolites, such as cyclic nucleotides, to regulate cell functions. Alteratio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22140

    authors: Ciarcia R,d'Angelo D,Pacilio C,Pagnini D,Galdiero M,Fiorito F,Damiano S,Mattioli E,Lucchetti C,Florio S,Giordano A

    更新日期:2010-08-01 00:00:00