A toxin-resistant mouse L-cell mutant defective in protein transport along the secretory pathway.


:Using methods designed for isolation of mutants defective in receptor-mediated endocytosis, a novel L-cell mutant was obtained that exhibits resistance to three different protein toxins as well as alterations in secretion. This mutant, LEFIC, is resistant to modeccin, Pseudomonas exotoxin, and ricin. These toxins, which enter the cytoplasm via receptor-mediated endocytosis, are thought to penetrate into cells at the level of late endosomes or the trans Golgi network. Early endosomal acidification appears to be normal in the mutant based on its accumulation of iron from transferrin and its sensitivity to diphtheria toxin A chain-transferrin conjugate. Within the secretory pathway two delays in transport of vesicular stomatitis virus (VSV) G protein were observed in LEFIC: a 20-30 min delay in acquisition of Endo H resistance and a 1-2 hr delay in appearance of newly synthesized G protein on the cell surface. Movement of endogenous proteins along the secretory pathway was also affected in LEFIC. Fibronectin secretion was delayed by 15 min, and membrane proteins were delayed in arrival at the cell surface. The phenotype of LEFIC is consistent with a defect in a component or compartment shared by both the late endocytic and constitutive secretory pathways.


J Cell Physiol


Laurie SM,Robbins AR





Has Abstract


1991-05-01 00:00:00












  • In Vitro and In Vivo Differentiation of Progenitor Stem Cells Obtained After Mechanical Digestion of Human Dental Pulp.

    abstract::Human population is facing a revolutionary change in the demographic structure with an increasing number of elderly people requiring an unmet need to ensure a smooth aging process and dental care is certainly an important aspect that has to be considered. To date, dentistry has been conservative and the need of transf...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Monti M,Graziano A,Rizzo S,Perotti C,Del Fante C,d'Aquino R,Redi CA,Rodriguez Y Baena R

    更新日期:2017-03-01 00:00:00

  • Cadmium induces cell centrosome amplification via reactive oxygen species as well as endoplasmic reticulum stress pathway.

    abstract::There is evidence that cadmium can initiate carcinogenesis. However, the underlying mechanisms remain unknown. There is also evidence that moderate centrosome amplification can initiate tumorigenesis. The present study investigated whether cadmium could trigger cell centrosome amplification, and examined the underlyin...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang RK,Wang P,Lu YC,Lang L,Wang L,Lee SC

    更新日期:2019-08-01 00:00:00

  • miR-335-5p induces insulin resistance and pancreatic islet β-cell secretion in gestational diabetes mellitus mice through VASH1-mediated TGF-β signaling pathway.

    abstract::Multiple studies have reported different methods in treating gestational diabetes mellitus (GDM); however, the relationship between miR-335-5p and GDM still remains unclear. Here, this study explores the effect of miR-335-5p on insulin resistance and pancreatic islet β-cell secretion via activation of the TGFβ signali...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tang XW,Qin QX

    更新日期:2019-05-01 00:00:00

  • Heat shock protein (Hsp) regulation by muscarinic acetylcholine receptor (mAChR) activation in the rat hippocampus.

    abstract::The cholinergic system plays a crucial role in modulating in the central nervous system physiological responses such as neurogenesis, neuronal differentiation, synaptic plasticity, and neuroprotection. In a recent study, we showed that Oxotremorine-M, a non-selective muscarinic acetylcholine receptor agonist, is able ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Frinchi M,Scaduto P,Cappello F,Belluardo N,Mudò G

    更新日期:2018-08-01 00:00:00

  • A simple approach for multicolor immunofluorescence staining in different Drosophila cell types.

    abstract::Multicolor immunostaining analysis is often a desirable tool in cell biology for most researchers. Nonetheless, this is not an easy task and often not affordable by many laboratories as it might require expensive instrumentation and sophisticated analysis software. Here, we describe a simple protocol for performing se...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cipressa F,Di Giorgio ML,Cenci G

    更新日期:2014-06-01 00:00:00

  • The STAT3/NFIL3 signaling axis-mediated chemotherapy resistance is reversed by Raddeanin A via inducing apoptosis in choriocarcinoma cells.

    abstract::Chemotherapy resistance is the major issue of choriocarcinoma. Apoptosis always is the ultimate outcome of chemotherapeutic drugs, which considered one of the reasons of resistance. We investigated the role of STAT3/NFIL3 signaling-inhibited apoptosis in chemotherapy resistance and whether Raddeanin A (RA) could be a ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Peng Z,Zhang C,Zhou W,Wu C,Zhang Y

    更新日期:2018-07-01 00:00:00

  • Effects of pH-variations on the kinetics of growth and energy metabolism in cultured T-lymphoma cells: a microcalorimetric study.

    abstract::The progression of T-lymphoma cells (CCRF-CEM) growing in suspension has been monitored during long term (12-28 h) batch experiments using microcalorimetry. In parallel with the calorimetric measurements, changes in cell concentration, pH, p(O2) and concentrations of the main energy sources (glucose and glutamine) wer...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bäckman P,Kimura T,Schön A,Wadsö I

    更新日期:1992-01-01 00:00:00

  • AKT and MAPK signaling in KGF-treated and UVB-exposed human epidermal cells.

    abstract::Regulation of proliferation and differentiation in keratinocyte is a complex and dynamic process that involves activation of multiple signaling pathways triggered by different growth factors. Keratinocyte growth factor (KGF) is not only a potent mitogen, but differently from other growth factors, is a potent inducer o...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lotti LV,Rotolo S,Francescangeli F,Frati L,Torrisi MR,Marchese C

    更新日期:2007-09-01 00:00:00

  • Adenosine receptor-mediated changes in cyclic AMP production and DNA synthesis in cultured arterial smooth muscle cells.

    abstract::The effects of adenosine and two analogs, L-phenylisopropyladenosine (L-PIA) and 5'-N-ethylcarboxamidoadenosine (NECA), on cAMP production and on platelet-derived growth factor (PDGF)-stimulated initiation of DNA synthesis in growth-arrested cultures of rat arterial smooth muscle cells (SMC) were studied. The intracel...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Jonzon B,Nilsson J,Fredholm BB

    更新日期:1985-09-01 00:00:00

  • Cytomatrix synthesis in MDCK epithelial cells.

    abstract::Detailed information regarding the synthesis rates of individual protein components is important in understanding the assembly and dynamics of the cytoskeletal matrix of eukaryotic cells. As an approach to this topic, the dual isotope technique of Clark and Zak (J. Biol. Chem., 256:4863-4870, 1981), was employed to me...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mitchell JJ,Low RB,Woodcock-Mitchell JL

    更新日期:1990-06-01 00:00:00

  • C-C motif chemokine ligand 2 regulates lps-induced inflammation and ER stress to enhance proliferation of bovine endometrial epithelial cells.

    abstract::Chemokines play an important role in regulating the complex immune system at the maternal-fetal interface during pregnancy. Among various chemokines, CC motif chemokine ligand 2 (CCL2) plays a role in the recruitment of immune regulatory cells to implantation sites within the endometrium. In cattle, CCL2 is abundantl...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lim W,Bae H,Bazer FW,Kim SM,Song G

    更新日期:2018-04-01 00:00:00

  • Biological roles of glial fibrillary acidic protein as a biomarker in cartilage regenerative medicine.

    abstract::Glial fibrillary acidic protein (GFAP) is an intermediate filament that is expressed in specifically expressed auricular chondrocytes, which are good cell sources of cartilage regenerative medicine. Although our group uses GFAP as a biomarker of matrix production in the cultured auricular chondrocytes, the biological ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kanazawa S,Nishizawa S,Takato T,Hoshi K

    更新日期:2017-11-01 00:00:00

  • Proteasome inhibitor MG132 induces BAG3 expression through activation of heat shock factor 1.

    abstract::BAG3 protein, a member of the BAG co-chaperones family, sustains cell survival in a variety of normal and neoplastic cell types, via its interaction with a variety of partners, such as the heat shock protein (HSP) 70, Bcl-2, Raf-1 and others. Expression of BAG3 is induced by some stressful stimuli, such as heat shock,...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Du ZX,Zhang HY,Meng X,Gao YY,Zou RL,Liu BQ,Guan Y,Wang HQ

    更新日期:2009-03-01 00:00:00

  • EMAST status as a beneficial predictor of fluorouracil-based adjuvant chemotherapy for Stage II/III colorectal cancer.

    abstract:BACKGROUND:Elevated microsatellite alteration at selected tetranucleotide repeats (EMAST) is a type of microsatellite instability that occurs in ∼60% of colorectal cancers (CRCs) and associated with MSH3 dysfunction. A 5-fluorouracil (5-FU)-related cytotoxicity is attenuated in MSH3-deficient colon cancer cells. Report...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mohammadpour S,Goodarzi HR,Jafarinia M,Porhoseingholi MA,Nazemalhosseini-Mojarad E

    更新日期:2020-04-01 00:00:00

  • Effects of amphotericin B on ion transport proteins in airway epithelial cells.

    abstract::Topical intranasal application of the antifungal Amphotericin B (AmphoB) has been shown as an effective medical treatment of chronic rhinosinusitis. Because this antibiotic forms channels in lipid membranes, we considered the possibility that it affects the properties and/or cell surface expression of ion channels/pum...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Jornot L,Rochat T,Caruso A,Lacroix JS

    更新日期:2005-09-01 00:00:00

  • Inhibition of PI 3-kinase and RAS blocks IGF-I and insulin-induced uncoupling protein 1 gene expression in brown adipocytes.

    abstract::Fetal brown adipocytes expressed uncoupling protein 1 (UCP1) mRNA, this expression being blunted throughout culture for 24 h in a serum-free medium. At physiological doses, either insulin-like growth factor I (IGF-I) or insulin turned out to be as potent as dibutyryl cAMP (dbcAMP) in increasing UCP1 gene transcription...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Teruel T,Valverde AM,Navarro P,Benito M,Lorenzo M

    更新日期:1998-07-01 00:00:00

  • Evaluation of the viability of cultured corneal endothelial cells by quantitative electron probe X-ray microanalysis.

    abstract::Construction of artificial organs and tissues by tissue engineering is strongly dependent on the availability of viable cells. For that reason, the viability and the physiological status of cells kept in culture must be evaluated before the cells can be used for clinical purposes. In this work, we determined the viabi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Alaminos M,Sanchez-Quevedo MC,Muñoz-Avila JI,García JM,Crespo PV,González-Andrades M,Campos A

    更新日期:2007-06-01 00:00:00

  • Toll-like receptors: A pathway alluding to cancer control.

    abstract::Toll-like receptors (TLRs) are usually expressed on immune cells such as macrophages, dendritic cells, mast cells, as well as on eosinophils and some epithelial cells. They play a central role in the recognition of harmful molecules that belong to invading microorganisms or internal damaged tissues, which lead to infl...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Ayala-Cuellar AP,Cho J,Choi KC

    更新日期:2019-12-01 00:00:00

  • Apelin/APJ system: A novel promising target for neurodegenerative diseases.

    abstract::Neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), and Huntington's disease (HD), are incurable diseases characterized by progressive loss of cognitive or motor function, which construct a serious threat to the life quality of aging populations and their life spans. Apelin is an ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Luo H,Han L,Xu J

    更新日期:2020-02-01 00:00:00

  • Calcium uptake by isolated rat intestinal cells.

    abstract::Intestinal cells were isolated by a combination of mechanical and enzymatic means, and their calcium uptake was assayed by a rapid filtration procedure. Calcium uptake was a time- and concentration-dependent process that was markedly elevated at 25 and 37 degrees C, as compared to 0 degree C. Cells isolated from rat d...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bronner F,Pansu D,Bosshard A,Lipton JH

    更新日期:1983-09-01 00:00:00

  • Isoprenylated proteins in cultured cells: subcellular distribution and changes related to altered morphology and growth arrest induced by mevalonate deprivation.

    abstract::In the presence of lovastatin (mevinolin), an inhibitor of endogenous mevalonate synthesis, C1300 murine neuroblastoma cells incorporated (2-14C)mevalonate into several discrete polypeptides that were separable by SDS-PAGE. The electrophoretic pattern of the labeled proteins did not vary substantially when cells were ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Maltese WA,Sheridan KM

    更新日期:1987-12-01 00:00:00

  • P2Y2 receptor promotes intestinal microtubule stabilization and mucosal re-epithelization in experimental colitis.

    abstract::P2Y(2) receptor expression is increased in intestinal epithelial cells (IECs) during inflammatory bowel diseases (IBDs). In this context, P2Y(2) stimulates PGE(2) release by IECs, suggesting a role in wound healing. For this study, we have used the non-cancerous IEC-6 cell line. IEC-6 cell migration was determined usi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Degagné E,Degrandmaison J,Grbic DM,Vinette V,Arguin G,Gendron FP

    更新日期:2013-01-01 00:00:00

  • An anti-apoptotic protein, Hax-1, inhibits the HIV-1 rev function by altering its sub-cellular localization.

    abstract::The human immunodeficiency virus type 1 (HIV-1) Rev protein facilitates the nuclear export of viral mRNA containing the Rev response element (RRE). Although several host proteins co-operating with Rev in viral RNA export have been reported, little is known about the innate host defense factors that Rev overcomes to me...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Modem S,Reddy TR

    更新日期:2008-01-01 00:00:00

  • Norepinephrine decreases EGF binding in primary rat hepatocyte cultures.

    abstract::Norepinephrine (NE) produced a dose-dependent inhibition of 125I-epidermal growth factor (EGF) binding to adult rat hepatocytes in primary culture. This effect was maximal after 1 hr of incubation with NE and could be blocked by the presence of an alpha 1-specific adrenergic receptor antagonist. The inhibition of bind...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cruise JL,Cotecchia S,Michalopoulos G

    更新日期:1986-04-01 00:00:00

  • Regulation of albumin expression in fetal rat hepatocytes cultured under proliferative conditions: role of epidermal growth factor and hormones.

    abstract::Sustained production of plasma proteins, notably albumin, is a reliable indicator of the differentiated state of hepatocytes. In this work, we have developed a fetal hepatocyte culture system where studying the regulation of albumin expression in proliferating liver cells. Our results show that under proliferative con...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: de Juan C,Benito M,Fabregat I

    更新日期:1992-07-01 00:00:00

  • Retinoic acid downregulates growth, fibronectin and RAR alpha in 3T3 cells: Ha-ras blocks this response and RA metabolism.

    abstract::Retinoic acid (RA) reduced growth, fibronectin, and retinoic acid receptor (RAR alpha) in NIH 3T3 cells but not in cells transformed by the Ha-ras oncogene. RA lowered RAR alpha transcript and protein, increased RAR beta transcripts, and had no effect on RAR gamma. H-ras transformation downregulated RAR expression and...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: De Luca LM,Scita G,Takatsuka J

    更新日期:1997-11-01 00:00:00

  • Accumulation of polyploid cells and G2-phase cells during ascites tumor growth.

    abstract::Ehrlich ascites tumor cells from the plateau phase of growth were transplanted into new hosts, pulse-labeled with tritiated thymidine and blocked with repeated injections of vinblastine. When unlabeled cells were analyzed for their cellular DNA content utilizing a cytophotometric technique it was found that in relatio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Andersson G

    更新日期:1977-02-01 00:00:00

  • Identification and characterization of circular RNAs involved in mechanical force-induced periodontal ligament stem cells.

    abstract::Circular RNAs (circRNAs) play critical roles in signal transduction during cell proliferation, differentiation, and apoptosis in a posttranscriptional manner. Recently, circRNAs have been proved to be a large class of animal RNAs with regulatory potency. However, whether circRNAs can respond to mechanical force (MF) a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wang H,Feng C,Jin Y,Tan W,Wei F

    更新日期:2019-07-01 00:00:00

  • Wheat germ agglutinin stains dispersed post-golgi vesicles after treatment with the cytokinesis inhibitor psychosine.

    abstract::The galactosylsphingosine psychosine (Psy) is one of the sphingolipids and induce the formation of multinuclear cells in several cell lines by inhibiting cytokinesis. In the present report, we show that intracellular organelles, including wheat germ agglutinin (WGA)-positive vesicles and early endosomes, are selective...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kanazawa T,Takematsu H,Yamamoto A,Yamamoto H,Kozutsumi Y

    更新日期:2008-05-01 00:00:00

  • An Overview on Predictive Biomarkers of Testicular Germ Cell Tumors.

    abstract::Testicular germ cell tumors (TGCTs) are frequent solid malignant tumors and cause of death in men between 20-40 years of age. Genetic and environmental factors play an important role in the origin and development of TGCTs. Although the majority of TGCTs are responsive to chemotherapy, about 20% of patient presents inc...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Chieffi P

    更新日期:2017-02-01 00:00:00