Functional alpha1- and beta2-adrenergic receptors in human osteoblasts.

Abstract:

:Central (hypothalamic) control of bone mass is proposed to be mediated through beta2-adrenergic receptors (beta2-ARs). While investigations in mouse bone cells suggest that epinephrine enhances both RANKL and OPG mRNA via both beta-ARs and alpha-ARs, whether alpha-ARs are expressed in human bone cells is controversial. The current study investigated the expression of alpha1-AR and beta2-AR mRNA and protein and the functional role of adrenergic stimulation in human osteoblasts (HOBs). Expression of alpha1B- and beta2-ARs was examined by RT-PCR, immunofluorescence microscopy and Western blot (for alpha1B-ARs). Proliferation in HOBs was assessed by (3)H-thymidine incorporation and expression of RANKL and OPG was determined by quantitative RT-PCR. RNA message for alpha1B- and beta2-ARs was expressed in HOBs and MG63 human osteosarcoma cells. alpha1B- and beta2-AR immunofluorescent localization in HOBs was shown for the first time by deconvolution microscopy. alpha1B-AR protein was identified in HOBs by Western blot. Both alpha1-agonists and propranolol (beta-blocker) increased HOB replication but fenoterol, a beta2-agonist, inhibited it. Fenoterol nearly doubled RANKL mRNA and this was inhibited by propranolol. The alpha1-agonist cirazoline increased OPG mRNA and this increase was abolished by siRNA knockdown of alpha1B-ARs in HOBs. These data indicate that both alpha1-ARs and beta2-ARs are present and functional in HOBs. In addition to beta2-ARs, alpha1-ARs in human bone cells may play a role in modulation of bone turnover by the sympathetic nervous system.

journal_name

J Cell Physiol

authors

Huang HH,Brennan TC,Muir MM,Mason RS

doi

10.1002/jcp.21761

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

267-75

issue

1

eissn

0021-9541

issn

1097-4652

journal_volume

220

pub_type

杂志文章
  • Methylation of CLEC14A is associated with its expression and lung adenocarcinoma progression.

    abstract::Our main objective is probing the effect of methylation of CLEC14A on its expression and lung adenocarcinoma (LUAD) progression. Microarray analysis was utilized to screen out differentially downregulated genes with hypermethylation in LUAD tissues. The CLEC14A expression level was measured by western blot analysis an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27112

    authors: Su C,Shi K,Cheng X,Han Y,Li Y,Yu D,Liu Z

    更新日期:2019-03-01 00:00:00

  • The suppressive effect of myeloid Elf-1-like factor (MEF) in osteogenic differentiation.

    abstract::Myeloid Elf-1 like factor (MEF) is a member of the Ets transcription factor family. Ets family proteins control the expression of genes that are critical for biological processes such as proliferation, differentiation, and cell death. Some of Ets factors are also known to regulate bone development. In this study, we i...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20933

    authors: Kim YJ,Kim BG,Lee SJ,Lee HK,Lee SH,Ryoo HM,Cho JY

    更新日期:2007-04-01 00:00:00

  • Stable variant of LM fibroblast defective in fluid-phase but competent in receptor-mediated endocytosis.

    abstract::The F-40 cell line, a stable variant of LM fibroblasts selected for its resistance to polyethylene glycol (PEG)-induced fusion (Roos and Davidson: Somatic Cell and Molecular Genetics 6:381-391, 1980), has a decreased capacity to internalize fluid-phase markers and nonspecifically surface-bound macromolecules. It is no...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041370314

    authors: Ryser HJ,Li W,Mandel R,Shen WC

    更新日期:1988-12-01 00:00:00

  • Biological effects of insulin and its analogs on cancer cells with different insulin family receptor expression.

    abstract::Hyperinsulinemia is a likely cause of the increased cancer incidence and mortality in diabetic patients, but its role is difficult to define in vivo. Previous in vitro studies testing the mitogenic potential of insulin and its analogs provided incomplete and sometimes contradictory results. To better evaluate cancer c...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24635

    authors: Sciacca L,Cassarino MF,Genua M,Vigneri P,Giovanna Pennisi M,Malandrino P,Squatrito S,Pezzino V,Vigneri R

    更新日期:2014-11-01 00:00:00

  • Pmepa1 induced by RANKL-p38 MAPK pathway has a novel role in osteoclastogenesis.

    abstract::Osteoclasts are multinucleated cells formed by fusion of preosteoclasts (POCs) derived from cells of the monocyte/macrophage lineage. We have reported a culture system that supports the formation of POCs from stroma-depleted rat bone marrow cells. Global gene expression analysis of this culture system identified genes...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26147

    authors: Funakubo N,Xu X,Kukita T,Nakamura S,Miyamoto H,Kukita A

    更新日期:2018-04-01 00:00:00

  • Low oxygen tension stimulates collagen synthesis and COL1A1 transcription through the action of TGF-beta1.

    abstract::Recent findings point to low oxygen tension (hypoxia) as an important mechanism for the expression of several eukaryotic genes. We have previously shown that hypoxia (2% O2), when compared to standard oxygen tension (20% O2), upregulates the mRNA levels of the human alpha1(I) (COL1A1) procollagen gene and transforming...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.10065

    authors: Falanga V,Zhou L,Yufit T

    更新日期:2002-04-01 00:00:00

  • Effect of aging on the mechanisms of PTH-induced calcium influx in rat intestinal cells.

    abstract::We have investigated the effects of aging on parathyroid hormone (PTH) modulation of intracellular calcium homeostasis and their relationship to signal transduction pathways in isolated rat duodenal cells (enterocytes). PTH (10(-8)-10(-9) M) increased enterocyte (45)Ca(2+) influx and intracellular Ca(2+) concentration...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(200003)182:3<429::AID-JCP1

    authors: Massheimer V,Picotto G,Boland R,De Boland AR

    更新日期:2000-03-01 00:00:00

  • Long noncoding RNA NONHSAG053901 promotes diabetic nephropathy via stimulating Egr-1/TGF-β-mediated renal inflammation.

    abstract::Diabetic nephropathy (DN) is an important factor leading to end-stage kidney disease that affects diabetes mellitus patients globally. Our previous transcriptome sequencing has identified a large group of differentially expressed long noncoding RNA (lncRNA) in early development of DN. On basis of this, we aimed to inv...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28485

    authors: Peng W,Huang S,Shen L,Tang Y,Li H,Shi Y

    更新日期:2019-08-01 00:00:00

  • The effect of metabolic inhibition on ion contents and sodium exchange in human lymphocytes.

    abstract::Lymphocytes depleted of ATP by incubation in iodoacetate (IAA) and nitrogen (N2) lost K and gained Na. Isotopic Na exchange showed a fast fraction and a slower exponential fraction, the latter conventionally assumed to reflect surface membrane properties. The gain of cell Na was not accounted for by a decrease in 22Na...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041100312

    authors: Negendank W,Shaller C

    更新日期:1982-03-01 00:00:00

  • Reduced cardiomyocyte Na+ current in the age-dependent murine Pgc-1β-/- model of ventricular arrhythmia.

    abstract::Peroxisome proliferator-activated receptor-γ coactivator-1 deficient (Pgc-1β-/- ) murine hearts model the increased, age-dependent, ventricular arrhythmic risks attributed to clinical conditions associated with mitochondrial energetic dysfunction. These were accompanied by compromised action potential (AP) upstroke ra...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27183

    authors: Ahmad S,Valli H,Smyth R,Jiang AY,Jeevaratnam K,Matthews HR,Huang CL

    更新日期:2019-04-01 00:00:00

  • Assembly of the peripheral domain of the bovine vacuolar H(+)-adenosine triphosphatase.

    abstract::The biosynthesis and assembly of the peripheral sector (V1) of the vacuolar proton-translocating adenosine triphosphatase (V-ATPase) was studied in a bovine kidney epithelial cell line. Monolayer cultures of cells were metabolically radiolabeled with Tran35S-label and the V-ATPase subsequently immunoprecipitated using...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041560106

    authors: Myers M,Forgac M

    更新日期:1993-07-01 00:00:00

  • p53-mediated regulation of cell cycle progression: pronounced impact of cellular microenvironment.

    abstract::Data on the biological effects of some overexpressed oncogenes and their cooperation with cellular factors are, at least partially, contradictory. There are reports on the strong pro-apoptotic action of temperature-sensitive (ts) p53(135val) in transformed cells at permissive temperature. However, in our experience ve...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21705

    authors: Schmid G,Kramer MP,Wesierska-Gadek J

    更新日期:2009-05-01 00:00:00

  • MicroRNAs in alcoholic liver disease: Recent advances and future applications.

    abstract::Alcoholic liver disease (ALD) is characterized by hepatocyte damage, inflammatory cell activation, and increased intestinal permeability leading to the clinical manifestations of alcoholic hepatitis. Selected members of the family of microRNAs (miRNAs) are affected by alcohol, resulting in an abnormal miRNA profile in...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.26938

    authors: Xu T,Li L,Hu HQ,Meng XM,Huang C,Zhang L,Qin J,Li J

    更新日期:2018-01-01 00:00:00

  • EMAST status as a beneficial predictor of fluorouracil-based adjuvant chemotherapy for Stage II/III colorectal cancer.

    abstract:BACKGROUND:Elevated microsatellite alteration at selected tetranucleotide repeats (EMAST) is a type of microsatellite instability that occurs in ∼60% of colorectal cancers (CRCs) and associated with MSH3 dysfunction. A 5-fluorouracil (5-FU)-related cytotoxicity is attenuated in MSH3-deficient colon cancer cells. Report...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29249

    authors: Mohammadpour S,Goodarzi HR,Jafarinia M,Porhoseingholi MA,Nazemalhosseini-Mojarad E

    更新日期:2020-04-01 00:00:00

  • Coordinate secretion of rat and mouse albumin by mouse hepatoma x rat hepatoma hybrid cells directly reflects the intracellular concentration of the corresponding mRNAs.

    abstract::The ratio of mouse to rat albumin secreted by mouse hepatoma x rat hepatoma hybrid cells is constant (of the order of 5.0) irrespective of the total amounts produced. The present results establish for seven independent hybrid clones that the coordination in the ratio of mouse to rat product applies also at the level o...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041150211

    authors: Sellem CH,Cassio D,Sala-Trepat JM,Weiss MC

    更新日期:1983-05-01 00:00:00

  • Homocysteine-induced myofibroblast differentiation in mouse aortic endothelial cells.

    abstract::Differentiation of myofibroblast, as evidenced by alpha-smooth muscle actin (alpha-SMA) expression, is largely mediated by transforming growth factor-beta1 (TGF-beta1). This mechanism often follows inflammatory events such as endothelial damage due to oxidative stress, which can further leads to vascular thickening, s...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20752

    authors: Sen U,Moshal KS,Tyagi N,Kartha GK,Tyagi SC

    更新日期:2006-12-01 00:00:00

  • Hypoxia/hypercapnia-induced adaptation maintains functional capacity of cord blood stem and progenitor cells at 4°C.

    abstract::We analyzed the effect of exposure to hypoxic/hypercapnic (HH) gas mixture (5% O2 /9% CO2 ) on the maintenance of functional cord blood CD34(+) hematopoietic stem and progenitor cells in severe hypothermia (4°C) employing the physiological and proteomic approaches. Ten-day exposure to HH maintained the Day 0 (D-0) lev...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24678

    authors: Vlaski M,Negroni L,Kovacevic-Filipovic M,Guibert C,Brunet de la Grange P,Rossignol R,Chevaleyre J,Duchez P,Lafarge X,Praloran V,Schmitter JM,Ivanovic Z

    更新日期:2014-12-01 00:00:00

  • Versatile role of curcumin and its derivatives in lung cancer therapy.

    abstract::Lung cancer is a main cause of death all over the world with a high incidence rate. Metastasis into neighboring and distant tissues as well as resistance of cancer cells to chemotherapy demand novel strategies in lung cancer therapy. Curcumin is a naturally occurring nutraceutical compound derived from Curcuma longa (...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.29819

    authors: Ashrafizadeh M,Najafi M,Makvandi P,Zarrabi A,Farkhondeh T,Samarghandian S

    更新日期:2020-12-01 00:00:00

  • Pulsatile and steady flow induces c-fos expression in human endothelial cells.

    abstract::The effects of pulsatile and steady fluid flow on the mRNA levels of proto-oncogenes c-fos, c-jun, and c-myc in cultured human umbilical vein endothelial cells (HUVEC) were investigated. c-fos mRNA levels in stationary cultures were very low. A 1 Hz pulsatile flow with an average shear stress of 16 dynes/cm2 induced a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041540118

    authors: Hsieh HJ,Li NQ,Frangos JA

    更新日期:1993-01-01 00:00:00

  • β Common receptor integrates the erythropoietin signaling in activation of endothelial nitric oxide synthase.

    abstract::Erythropoietin (EPO), the key hormone for erythropoiesis, also increases nitric oxide (NO) bioavailability in endothelial cells (ECs), yet the definitive mechanisms are not fully understood. Increasing evidence has demonstrated that β common receptor (βCR) plays a crucial role in EPO-mediated non-hematopoietic effects...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22678

    authors: Su KH,Shyue SK,Kou YR,Ching LC,Chiang AN,Yu YB,Chen CY,Pan CC,Lee TS

    更新日期:2011-12-01 00:00:00

  • Essential Role of CFTR in PKA-Dependent Phosphorylation, Alkalinization, and Hyperpolarization During Human Sperm Capacitation.

    abstract::Mammalian sperm require to spend a limited period of time in the female reproductive tract to become competent to fertilize in a process called capacitation. It is well established that HCO3- is essential for capacitation because it activates the atypical soluble adenylate cyclase ADCY10 leading to cAMP production, an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25634

    authors: Puga Molina LC,Pinto NA,Torres Rodríguez P,Romarowski A,Vicens Sanchez A,Visconti PE,Darszon A,Treviño CL,Buffone MG

    更新日期:2017-06-01 00:00:00

  • Biosensors for real-time monitoring of physiological processes in the musculoskeletal system: A systematic review.

    abstract::Biosensors are composed of (bio)receptors, transducers, and detection systems and are able to convert the biological stimulus into a measurable signal. This systematic review evaluates the current state of the art of innovation and research in this field, identifying the biosensors that in vitro monitor the musculoske...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28753

    authors: Veronesi F,Tschon M,Visani A,Fini M

    更新日期:2019-12-01 00:00:00

  • Differences of SiHa (human cancer of cervix) and BMG-1 (brain glioma) cell lines as 2D and 3D cultures.

    abstract::Cell cultures have seen much progress in the numbers available cell lines, their applications and culture techniques. Three dimensional cultures and co-cultures are examples of strategies that bring in vitro conditions closer to natural in vivo systems. We describe here, the formation of cell aggregates in three-dimen...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24433

    authors: Ravi M,Sah S,Bhammar R

    更新日期:2014-02-01 00:00:00

  • Growth kinetics as a function of ploidy in diploid, tetraploid, and octaploid smooth muscle cells derived from the normal rat aorta.

    abstract::The smooth muscle cell population in major arteries of humans and experimental animals is heterogeneous with regard to cellular DNA content. A proportion of cells has polyploid DNA content and this proportion increases with normal aging and with hypertension. We have isolated pure populations of rat aortic smooth musc...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041250322

    authors: Rosen EM,Goldberg ID,Shapiro HM,Zoller LC,Myrick KV,Levenson SE,Halpin PA

    更新日期:1985-12-01 00:00:00

  • Light Sheet Microscopy to Measure Protein Dynamics.

    abstract::Visualizing protein dynamics is the key to a quantitative understanding of molecular mechanisms in biological systems. Recent developments in fluorescent microscopy techniques allow for novel experimental approaches to study stochastic localization, distribution, and movement of fluorescently labeled molecules in high...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25451

    authors: Rieckher M

    更新日期:2017-01-01 00:00:00

  • Thrombin-induced thromboxane generation by neutrophils and lymphocytes: dependence on enzymic site.

    abstract::We examined the effects of thrombin on thromboxane generation by sheep neutrophils and lymphocytes in vitro. Physiological concentration of thrombin (50 nM) resulted in thromboxane B2 generation from both neutrophils and lymphocytes, which was comparable to that obtained with zymosan activated serum challenge of the c...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041320224

    authors: Bizios R,Lai L,Fenton JW 2nd,Malik AB

    更新日期:1987-08-01 00:00:00

  • Ascorbic Acid Induces Necrosis in Human Laryngeal Squamous Cell Carcinoma via ROS, PKC, and Calcium Signaling.

    abstract::Ascorbic acid induces apoptosis, autophagy, and necrotic cell death in cancer cells. We investigated the mechanisms by which ascorbic acid induces death in laryngeal squamous cell carcinoma Hep2 cells. Ascorbic acid markedly reduced cell viability and induced death without caspase activation and an increase in cytochr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25438

    authors: Baek MW,Cho HS,Kim SH,Kim WJ,Jung JY

    更新日期:2017-02-01 00:00:00

  • Role of serum in the developmental expression of alkaline phosphatase in MC3T3-E1 osteoblasts.

    abstract::MC3T3-E1 cells in culture exhibit a temporal sequence of development similar to in vivo bone formation. To examine whether the developmental expression of the osteoblast phenotype depends on serum derived factors, we compared the time-dependent expression of alkaline phosphatase (ALP)-a marker of osteoblastic maturati...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041580311

    authors: Yohay DA,Zhang J,Thrailkill KM,Arthur JM,Quarles LD

    更新日期:1994-03-01 00:00:00

  • Isolation, Characterization, and Establishment of Spontaneously Immortalized Cell Line HRPE-2S With Stem Cell Properties.

    abstract::The retinal pigment epithelium is a monolayer of highly specialized pigmented cells located between the neural retina and the Bruch's membrane of the choroid. RPE cells play a crucial role in the maintenance and function of the underlying photoreceptors. This study introduces a spontaneously arising human retinal pigm...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25729

    authors: Shams Najafabadi H,Soheili ZS,Samiei S,Ahmadieh H,Ranaei Pirmardan E,Masoumi M

    更新日期:2017-10-01 00:00:00

  • Increased uptake of thymidine in the activation of sea urchin eggs. III. Effects of aphidicolin.

    abstract::Uptake and phosphorylation of externally supplied [3H]-thymidine are fully stimulated in fertilized sea urchin eggs exposed to 5.0 micrograms/ml aphidicolin. As in untreated controls, the rate of uptake in aphidicolin-treated eggs increases greater than 50-fold shortly after fertilization, and greater than 85% of the ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041180107

    authors: Nishioka D,Killian CE,Chacon CT,Sgagias MK

    更新日期:1984-01-01 00:00:00