Abstract:
:Rho is known as an important regulator of actin microfilament formation. We were led to study it because a dynamic rearrangement of actin filaments occurs during activation of gastric acid secretion. In order to use specific probes, the rabbit gastric gland culture system was employed and the various genes were expressed using adenovirus vector. When the constitutive active mutant of Rho (RhoAV14) was expressed, histamine- or carbachol-stimulated acid secretion monitored by (14)C-aminopyrine accumulation was inhibited. Conversely, expression of C3 toxin, the specific inhibitor of Rho, and expression of G(12/13)-specific regulator of G-protein signaling domain, the specific inhibitor of G(12/13) which is considered to be an upstream mediator of Rho, both potentiated acid secretion stimulated by the agonists. F-actin staining of parietal cell expressing RhoAV14 revealed that the microfilament supporting the intracellular canaliculi (not on the basolateral membrane) almost disappeared. No clear changes in the intracellular localization of Rho were observed during stimulation of parietal cell. In resting glands, the endogenous active form of Rho was relatively high, and it decreased during histamine stimulation. The finding that any treatment which inhibit Rho augment acid secretion whereas those that activate Rho inhibit secretion strongly suggests that the Rho-pathway conducts a negatively regulating signal in parietal cell activation, possibly via site-specific regulation of actin microfilaments.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Tashiro K,Nagao T,Kurose H,Ichijo H,Urushidani Tdoi
10.1002/jcp.10370keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
409-17issue
3eissn
0021-9541issn
1097-4652journal_volume
197pub_type
杂志文章abstract::Hemopoietic cell proliferation is controlled by a set of polypeptide growth factors and regulatory molecules that bind to cell surface receptors inducing cellular responses. Maintenance of a viable state, cell growth, DNA synthesis and mitosis are basic properties of proliferating cells, but links between growth facto...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041630306
更新日期:1995-06-01 00:00:00
abstract::Sirtuin 3 (SIRT3) a mitochondrial enzyme that plays an important role in energy homeostasis, cardiac remodeling, and heart failure (HF). The expression of SIRT3 declines with advanced age, cardiovascular, and metabolic diseases. Accumulating evidence suggests that SIRT3 plays a critical role in protecting the heart fr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.27200
更新日期:2019-03-01 00:00:00
abstract::Hemodynamic forces influence many endothelial cell functions. The coupling between hemodynamic forces and cell function could be mediated by mechano-sensitive ion channels present in the plasma membrane of endothelial cells. Because one of these channels is permeable to Ca2+, we tested whether hemodynamic forces influ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041510107
更新日期:1992-04-01 00:00:00
abstract::Comparative studies with rat transferrin (rTf) and asialotransferrin (asialo-rTf) were performed on suspended adult rat hepatocytes with the aim of elucidating the mechanism of enhanced hepatic Fe acquisition from asialo-rTf observed previously in vivo. At low ligand concentrations (0.05-20 micrograms/ml), the cells b...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041350325
更新日期:1988-06-01 00:00:00
abstract::The temperature-sensitive (ts) Chinese hamster ovary (CHO) cell mutant tsH1 contains a thermolabile leucyl-tRNA synthetase. Upon incubation at the nonpermissive temperature of 39.5 degrees C, the enzyme became reversibly inhibited over a period of minutes, and the cells lost viability over a period of many hours. Howe...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041400206
更新日期:1989-08-01 00:00:00
abstract::Cholangiocarcinoma (CCA) is a mortal cancer with gradually increasing incidences all over the world, whereas effective diagnosis and treatment for this disease are still lacking. As a classical long noncoding RNA (lncRNA), maternally expressed gene 3 (MEG3) has been reported to exhibit pivotal regulatory roles in the ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28856
更新日期:2019-12-01 00:00:00
abstract::Insufficient invasion ability of trophoblasts might be associated with the development of recurrent miscarriage (RM). Ubiquitin-specific protease 25 (USP25) can regulate the processes of invasion and migration in different types of cancer cells. However, the effect of USP25 on trophoblasts and its roles in the develop...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28593
更新日期:2019-11-01 00:00:00
abstract::Monocytes are recruited to the material surface of an implanted biomedical device recognizing it as a foreign body. Differentiation into macrophages subsequently occurs followed by fusion to form foreign body giant cells (FBGCs). Consequently, implants can become degraded, cause chronic inflammation or become isolated...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10412
更新日期:2004-04-01 00:00:00
abstract::Cultured preadipose 3T3 cells undergo a process of differentiation in which they convert to adipose cells. Growth hormone promotes this conversion. Since 3T3 sublines vary in their susceptibility to adipose conversion, it was of interest to examine the properties of the growth hormone receptors in relation to that sus...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041150312
更新日期:1983-06-01 00:00:00
abstract::Active oxygen species are generated in cells during pathophysiologic conditions such as inflammation and postischemic reperfusion. If oxygen radical scavengers are added before reperfusion, then the magnitude of injury is reduced. We investigated whether free radicals generated following exposure to hypoxia and reoxyg...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041560217
更新日期:1993-08-01 00:00:00
abstract::Epigallocatechin-3-gallate (EGCG) plays a crucial role in hepatic lipid metabolism. However, the underlying regulatory mechanism of hepatic lipid metabolism by EGCG in canine is unclear. Primary canine hepatocytes were treated with EGCG (0.01, 0.1, or 1 μM) and BML-275 (an AMP-activated protein kinase [AMPK] inhibitor...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29869
更新日期:2021-01-01 00:00:00
abstract::Myocardium infarction is one of the most deathly cardiovascular diseases. It is characterized by myocardium ischemia as a result of nutrients depletion and hypoxia. The cell can respond to this injury by autophagy or apoptosis, which determines the evolution and possible recovery of the myocardium infarction. Lipids p...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25344
更新日期:2016-10-01 00:00:00
abstract::Galectin-1 is reported to be upregulated in various human cancers. However, the relationship between galectin-1 expression and cancer prognosis has not been systematically assessed. In this study, we searched PubMed, Web of Science, and Embase to collect all relevant studies and a meta-analysis was performed. We found...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,meta分析
doi:10.1002/jcp.28059
更新日期:2019-08-01 00:00:00
abstract::Endothelial cell-derived growth factor (ECDGF) is a soluble mitogen secreted in vitro by bovine aortic endothelium. ECDGF is a mixture of at least two distinct heat-stable and trypsin-sensitive mitogens. Large amounts of mitogenic activity were found in lysates prepared from cultured endothelial cells. Other nonmitoge...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041210208
更新日期:1984-11-01 00:00:00
abstract::Aberrant proliferation of vascular smooth muscle cells (VSMC) is a critical contributor to the pathogenesis of atherosclerosis (AS). Our previous studies have demonstrated that apelin-13/APJ confers a proliferative response in VSMC, however, its underlying mechanism remains elusive. In this study, we aimed to investig...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27527
更新日期:2019-06-01 00:00:00
abstract::Cancer is a multifaceted disease that involves acquisition of genetic mutations, deletions, and amplifications as well as deregulation of epigenetic mechanisms that fine-tune gene regulation. Key epigenetic mechanisms that include histone modifications, DNA methylation, and non-coding RNA-mediated gene silencing are o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.24387
更新日期:2013-11-01 00:00:00
abstract::Multicolor immunostaining analysis is often a desirable tool in cell biology for most researchers. Nonetheless, this is not an easy task and often not affordable by many laboratories as it might require expensive instrumentation and sophisticated analysis software. Here, we describe a simple protocol for performing se...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24506
更新日期:2014-06-01 00:00:00
abstract::In recent years, research on glioma immunotherapy have grown rapidly. However, the autoimmune-like side effects that are caused by blocking immunological checkpoints hinder their clinical application in gliomas currently. Galectin-9, a ligand for T-cell immunoglobulin mucin 3, has shed a new light on the treatment of ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29309
更新日期:2020-05-01 00:00:00
abstract::Destructive bone diseases caused by osteolysis are increasing in incidence. They are characterized by an excessive imbalance of osteoclast formation and activation. During osteolysis, the activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways are triggered by receptor ac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27924
更新日期:2019-07-01 00:00:00
abstract::Although the mechanism is unknown, infiltration anesthetics are believed to have membrane-stabilizing action. We report here that such a most commonly used anesthetic, lidocaine, effectively inhibited the invasive ability of human cancer (HT1080, HOS, and RPMI-7951) cells at concentrations used in surgical operations ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10145
更新日期:2002-09-01 00:00:00
abstract::Primary astroglial cultures were used to compare the relationships to cell cycling of dolichol-linked glycoprotein synthesis, and of availability of mevalonate, the precursor of dolichol and other isoprenoid lipids. With shift-up to 10% serum (time 0) after 48 h of serum depletion, the proportion of cells in S phase (...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041490215
更新日期:1991-11-01 00:00:00
abstract::Current osteoinductive protein therapy utilizes bolus administration of large doses of bone morphogenetic proteins (BMPs), which is costly, and may not replicate normal bone healing. The limited in vivo biologic activity of BMPs requires the investigation of growth factors that may enhance this activity. In this study...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22639
更新日期:2011-11-01 00:00:00
abstract::The continuous discovery of human mesenchymal stem cells (hMSCs) in different tissues is stirring up a tremendous interest as a cell source for regenerative medicine therapies. Historically, hMSCs have been always considered a sub-population of mononuclear cells present in the bone marrow (BM). Although BM-hMSCs are s...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.24223
更新日期:2013-04-01 00:00:00
abstract::The present work aimed at determining whether interleukin-6 (IL-6) produced by skeletal muscle during exercise is related, at least partly, to calcineurin activity. Rats were treated with two specific calcineurin inhibitors, cyclosporin A (CsA) and FK506, or vehicle (Vhl); they were then subjected to exhaustive treadm...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20854
更新日期:2007-03-01 00:00:00
abstract::Exosomes extracted from mesenchymal stem cells (MSCs) was reported to reduce myocardial ischemia/reperfusion damage. Besides, stromal-derived factor 1 (SDF1a) functions as cardiac repair after myocardial infarction (MI). Therefore, the present study aims to identify whether exosomes (Exo) released from SDF1-overexpres...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28070
更新日期:2019-08-01 00:00:00
abstract::rac-1-O-Myristoyl-2-O-acetylglycerol, rac-1-O-palmitoyl-2-O-acetylglycerol, and rac-1-O-oleoyl-2-O-acetylglycerol acted like phorbol myristate acetate and mezerein in stimulating human neutrophil aggregation. Responses to these agents were equally influenced by cytochalasin B, extracellular calcium and magnesium, arac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041250204
更新日期:1985-11-01 00:00:00
abstract::The differentiation of human keratinocytes in most culture systems is incomplete; e.g., lamellar bodies, the characteristic lipid-delivery organelles of epidermis, are not present. Moreover, their lipid profile does not reflect the distinctive composition found in cornifying epidermis. In contrast, keratinocytes that ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041360113
更新日期:1988-07-01 00:00:00
abstract::Peroxisome proliferator activated receptors (PPARs, alpha, beta/delta, gamma) control lipid homeostasis and differentiation in various tissues and tumor cells. PPARbeta and PPARgamma increase oligodendrocyte maturation in glial mixed populations and spinal cord oligodendrocytes, respectively, and PPARbeta is known to ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21509
更新日期:2008-11-01 00:00:00
abstract::Epithelial-mesenchymal transition (EMT), via activation of Wnt signaling, is prevailing in embryogenesis, but postnatally it only occurs in pathological processes, such as in tissue fibrosis and tumor metastasis. Our prior studies led us to speculate that EMT might be involved in the loss of limbal epithelial stem cel...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24126
更新日期:2013-01-01 00:00:00
abstract::Several organic cations are actively transported by proximal renal tubules by mediated processes across both the apical and basolateral cell membranes. In order to evaluate this transport system in a cultured renal epithelium, uptake of 3H-tetraethylammonium (TEA) across the apical membrane was measured in LLCPK1 cell...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041370317
更新日期:1988-12-01 00:00:00