Abstract：
:The temperature-sensitive (ts) Chinese hamster ovary (CHO) cell mutant tsH1 contains a thermolabile leucyl-tRNA synthetase. Upon incubation at the nonpermissive temperature of 39.5 degrees C, the enzyme became reversibly inhibited over a period of minutes, and the cells lost viability over a period of many hours. However, killing of tsH1 by acute heating at 45 degrees C was identical to that of wild-type (SC) cells. In addition, the heat-induced inhibition of protein synthesis was similar for both cell types, as measured after acute heating at 45 degrees C. Furthermore, both killing and inhibition of protein synthesis showed thermotolerance in both cell types. In contrast to the effects at 45 degrees C, at 39.5 degrees C, neither the inhibition of leucyl-tRNA synthetase activity nor the killing of tsH1 expressed thermotolerance. Also, treatment of tsH1 at 39.5 degrees C did not induce thermotolerance to killing at 45 degrees C. The inhibition of leucyl-tRNA synthetase activity in tsH1 at 39.5 degrees C was further distinguished from the 45 degrees C-induced inhibition of protein synthesis in SC cells by a much more rapid reversal of the inhibition of leucyl-tRNA synthetase activity. Also, the rate of reversal of the inhibition of protein synthesis by 45 degrees C in SC cells was decreased by increased heat dose. Such was not true for the 39.5 degrees C inhibition of leucyl-tRNA synthetase activity in tsH1. The data indicate that there exist two distinct types of thermal inhibition--one slowly reversible type which was observed during and after heating at 45 degrees C and both induced and expressed thermotolerance, and a second, rapidly reversible type, which was evident only during heating of tsH1 at 39.5 degrees C and neither induced nor expressed thermotolerance.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Vidair CA,Dewey WCdoi
10.1002/jcp.1041400206subject
Has Abstractpub_date
1989-08-01 00:00:00pages
227-32issue
2eissn
0021-9541issn
1097-4652journal_volume
140pub_type
杂志文章abstract：:We have described recently that expression of CD44 exon v10 (CD44v10) is down-regulated upon metastasis of squamous cell carcinoma, whereas it is up-regulated in skin metastases of malignant melanoma. The striking regulation of CD44v10 prompted us to generate a murine CD44v10-specific monoclonal antibody to define exp...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199706)171:3<305::AID-JCP9
更新日期：1997-06-01 00:00:00
abstract：:We have reported previously that the production of a tumor cell factor that stimulates synthesis of fibroblast collagenase is influenced by a fibroblast-deposited matrix component, possibly heparan sulfate-proteoglycan. In this study, binding sites for heparin and heparan sulfate on mouse B-16 melanoma cells have been...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041360119
更新日期：1988-07-01 00:00:00
abstract：:Regulation of proliferation and differentiation in keratinocyte is a complex and dynamic process that involves activation of multiple signaling pathways triggered by different growth factors. Keratinocyte growth factor (KGF) is not only a potent mitogen, but differently from other growth factors, is a potent inducer o...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21056
更新日期：2007-09-01 00:00:00
abstract：:With the long-range goals of elucidating the biochemical genetics and the phenotypic expression, both in vitro and in vivo, of mutator strains of mammalian cells, we have isolated and partially characterized a series of drug-resistant mutants from a subline a feeder-dependent parental mouse teratocarcinoma line (PSA-1...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041150103
更新日期：1983-04-01 00:00:00
abstract：:Keratin intermediate filaments play an important role in maintaining the integrity of the skin structure. Understanding the importance of this subject is possible with the investigation of keratin defects in epidermolysis bullosa simplex (EBS). Nowadays, in addition to clinical criteria, new molecular diagnostic metho...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.26898
更新日期：2018-01-01 00:00:00
abstract：:Cultured pig kidney cells designated LLC-PK1, previously shown to acquire Na+-dependent concentrative transport of hexoses as the cells become growth arrested, also show Na+-dependent concentrative uptake of the amino acid analogs alpha-aminoisobutyric acid (AIB) and (methyl) meAIB. This A system-like transport is mos...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041140207
更新日期：1983-02-01 00:00:00
abstract：:Ischemia is a situation occurring in several diseases including myocardial infarction and organ transplantation in which oxygenated blood supply is impaired. Ischemia leads to many cellular and tissue modifications, the most important one being cell death. Several explanations have been proposed to account for these m...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041520127
更新日期：1992-07-01 00:00:00
abstract：:The toxicity of cationic fluorescent dye, rhodamine 123, towards a number of independently established cell lines from three different species, namely human, mouse, and Chinese hamster, has been examined. All of the cell lines from any one species that were examined were found to exhibit similar sensitivities towards ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041300303
更新日期：1987-03-01 00:00:00
abstract：:Adhesion of calf lens epithelial cells to lens capsule, their natural basement membrane was found to be considerably more rapid than either to plastic or to type I or type IV collagen coated surfaces. No polarity of the basement membrane was observed as the cells were able to attach to either side of the anterior or p...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041130215
更新日期：1982-11-01 00:00:00
abstract：:Formation of human T lymphocyte colonies in semisolid medium from T lymphocyte colony-forming units (TL-CFUs) under stimulation of phytohemagglutinin (PHA) has been reported by several authors. These TL-CFUs were present in unsensitized lymphocyte populations. We report here that such TL-CFUs are capable of renewing t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041170114
更新日期：1983-10-01 00:00:00
abstract：:So far, there were no reports on circular RNA (circRNA) expression profiles in the differentiation of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) into cardiomyocyte-like cells induced by 5-aza. In this study, hUCMSCs were isolated from umbilical cords and induced with 5-aza for 14 days. Immunofluores...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28310
更新日期：2019-02-12 00:00:00
abstract：:Cell fusion occurs during fertilization and in the formation of organs such as muscles, placenta, and bones. We have developed an experimental model for epithelial cell fusion which permits analysis of the processes during junction disintegration and formation of polykaryons (Palovuori and Eskelinen [2000] Eur. J. Cel...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20024
更新日期：2004-09-01 00:00:00
abstract：:Cells of confluent cultures of the established pig renal epithelial line, LLC-PK1, accumulate alpha-methyl-D-glucoside against a concentration gradient. This transport system is strongly inhibited by phlorizin and 6-deoxy-D-glucose, moderately inhibited by phloretin, and only weakly inhibited by 3-0-methyl-D-glucose, ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041040311
更新日期：1980-09-01 00:00:00
abstract：:Skeletal muscle atrophy/wasting is associated with impaired protein metabolism in diverse physiological and pathophysiological conditions. Elevated levels of reactive oxygen species (ROS), disturbed redox status, and weakened antioxidant defense system are the major contributing factors toward atrophy. Regulation of p...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27348
更新日期：2019-05-01 00:00:00
abstract：:Evidence is emerging that long noncoding RNAs (lncRNAs) play vital roles in tumorigenesis. LncRNA gastric cancer-associated transcript 3 (GACAT3) is reported to participate in the development of breast cancer, colorectal cancer, nonsmall cell lung cancer, and gastric cancer. However, whether it is implicated in glioma...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27542
更新日期：2019-06-01 00:00:00
abstract：:During the development of atherosclerotic and fibromuscular proliferates/lesions, smooth muscle cells (SMC) in the media, particularly near the lumen, are activated to migrate into the intima, where they continue to proliferate to form an intimal thickening. It is to date unclear whether SMCs situated adjacent to the ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041470302
更新日期：1991-06-01 00:00:00
abstract：:Modeling breast cancer in the mouse has helped to better define the heterogeneity of human breast cancer. In the recent past, it has become evident that some limitations have restricted the potential benefits that can be achieved with this approach. In this review, we highlight some key points that should be taken int...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.24031
更新日期：2012-08-01 00:00:00
abstract：:Recently, after the identification of ferritin light chain (L-ferritin) gene and protein over-expression in human metastatic melanoma cells, we engineered, starting from the LM metastatic melanoma cell line, clones in which L-ferritin gene expression was down-regulated by the stable expression of a specific antisense ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20543
更新日期：2006-03-01 00:00:00
abstract：:The manipulation of autophagy provides a new opportunity for highly effective anticancer therapies. Recently, we showed that photodynamic therapy (PDT) with nitrogen-doped titanium dioxide (N-TiO2 ) nanoparticles (NPs) could promote the reactive oxygen species (ROS)-dependent autophagy in leukemia cells. However, the ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29479
更新日期：2020-11-01 00:00:00
abstract：:Beta-arrestins are scaffolding proteins implicated as negative regulators of TLR4 signaling in macrophages and fibroblasts. Unexpectedly, we found that beta-arrestin-1 (beta-arr-1) and -2 knockout (KO) mice are protected from TLR4-mediated endotoxic shock and lethality. To identify the potential mechanisms involved, w...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22289
更新日期：2010-11-01 00:00:00
abstract：:The roles of Sonic hedgehog (Shh) and Bone morphogenetic protein-2 (Bmp-2) in osteoblast differentiation were investigated using in vitro cell systems. Recombinant amino-terminal portion of SHH (rSHH-N) dose dependently stimulated ALP activity in C3H10T1/2 and MC3T3-E1 cells. rSHH-N induced expression of Osteocalcin m...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10166
更新日期：2002-11-01 00:00:00
abstract：:Radioiodinated granulocyte-macrophage colony-stimulating factor (125I-GM-CSF) binds to specific receptors (molecular weight approximately 50,000 daltons) on the murine myelomonocytic leukemia, WEHI-3BD+. At 4 degrees C 125I-GM-CSF remains on the surface of the cells and can be eluted by washing the cells with acidifie...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041300211
更新日期：1987-02-01 00:00:00
abstract：:Our objective was to establish the existence of intercellular communication through gap junctions in synovial lining cells and in primary and passaged cultures of human synovial cells. Communication between cells was assessed using the nystatin perforated-patch method, fluorescent dye transfer, immunochemistry, transm...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(200007)184:1<110::AID-JCP1
更新日期：2000-07-01 00:00:00
abstract：:Growing evidence from recent studies has shown that the X-inactive specific transcript (XIST), a well-known long noncoding RNA involved in early embryonic development, is aberrantly regulated in various human cancers. However, the prognostic value of XIST in cancers remains uncharacterized. In this study, we searched ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27400
更新日期：2019-05-01 00:00:00
abstract：:The development of spike potential mechanisms during cell differentiation was studied in chick myotubes formed in vitro from trypsin-dissociated myoblasts. The spike potential and its rate of rise were measured in myotubes from 4-14 day old cultures. A depolarizing current pulse was delivered to evoke the spike potent...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040900307
更新日期：1977-03-01 00:00:00
abstract：:Cultured preadipose 3T3 cells are able to undergo a process of differentiation through which they are converted into adipose cells. Growth hormone induces this conversion in resting cultures but not in growing cultures. It was of interest to determine the period of cell sensitivity to the hormone and the timing of the...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041280222
更新日期：1986-08-01 00:00:00
abstract：:Electrophysiologic properties of cultured human keratinocytes were studied using the patch voltage-clamp technique. Undifferentiated, proliferative keratinocytes grown in low Ca2+ medium had an average resting membrane potential of -24 mV. Voltage-clamp experiments showed that these cells had two membrane ionic curren...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041430103
更新日期：1990-04-01 00:00:00
abstract：:This study is performed to examine the impacts of microRNA-218 (miR-218) on cardiac microvascular endothelial cells (CMECs) injury induced by coronary artery disease (CAD). Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) was applied for detecting miR-218 expression in serum of patients with CAD ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29214
更新日期：2020-03-01 00:00:00
abstract：:The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21289
更新日期：2008-04-01 00:00:00
abstract：:The nuclear Ki-67 protein (pKi-67) has previously been shown to be exclusively expressed in proliferating cells. As a result, antibodies against this protein are widely used as prognostic tools in cancer diagnostics. Here we show, that despite the strong downregulation of pKi-67 expression in non-proliferating cells, ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20494
更新日期：2006-03-01 00:00:00
