Abstract:
:Cultured preadipose 3T3 cells undergo a process of differentiation in which they convert to adipose cells. Growth hormone promotes this conversion. Since 3T3 sublines vary in their susceptibility to adipose conversion, it was of interest to examine the properties of the growth hormone receptors in relation to that susceptibility. It was found that preadipose 3T3-F442A cells, which are able to convert to adipose cells with high frequency, are able to bind about 10(4) growth hormone molecules per cell with Kd approximately 10(-9) M. After adipose conversion, no appreciable change in hormone binding was detected. The binding of growth hormone to 3T3-C2 cells (a line virtually insusceptible to adipose conversion) was indistinguishable from that to 3T3-F442A cells. Internalization and degradation of the hormone were also similar in the two cell lines. Susceptibility to adipose conversion is therefore not determined by the relative ability of the cells to bind or degrade the hormone, but must instead depend on some response, as yet unidentified, that follows binding of the hormone.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Nixon T,Green Hdoi
10.1002/jcp.1041150312subject
Has Abstractpub_date
1983-06-01 00:00:00pages
291-6issue
3eissn
0021-9541issn
1097-4652journal_volume
115pub_type
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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pub_type: 杂志文章
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更新日期:1979-12-01 00:00:00
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更新日期:1977-09-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
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journal_title:Journal of cellular physiology
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更新日期:2010-07-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:1987-05-01 00:00:00