Dinoflagellate bioluminescence: a comparative study of invitro components.

Abstract:

:In vitro bioluminescence components of the dinoflagellates Gonyaulax polyedra, G. tamarensis, Dissodinium lunual, and Pyrocystis noctiluca were studied. The luciferases and luciferins of the four species cross-react in all combinations. All of these species possess high-molecular weight luciferases (200,000-400,000 daltons) with similar pH activity profiles. The active single chains of luciferases from the Gonyaulax species have a MW of 130,000 while those from P. noctiluca and D. lunula have a MW of 60,000. Extractable luciferase activity varies with time of day in the two Gonyaulax species, but not in the other two. A luciferin binding protein (LBP) can easily be extracted from the two Gonyaulax species (MW approximately 120,000 daltons), but none could be detected in extracts of either D. lunula or P. noctiluca. Scintillons are extractable from all four species, but they vary in density and the degree to which activity can be increased by added luciferin. Although the biochemistry of bioluminescence in these dinoflagellates is generally similar, the observations that D. lunula and P. noctiluca apparently lack LBP and have luciferases with low MW single chains require further clarification.

journal_name

J Cell Physiol

authors

Schmitter RE,Njus D,Sulzman FM,Gooch VD,Hastings JW

doi

10.1002/jcp.1040870115

keywords:

subject

Has Abstract

pub_date

1976-01-01 00:00:00

pages

123-34

issue

1

eissn

0021-9541

issn

1097-4652

journal_volume

87

pub_type

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