Depleted level of heparan sulfate proteoglycan in the extracellular matrix of endothelial cell cultures exposed to endotoxin.


:Exposure of cultured endothelial cells to endotoxin causes an increase in the amount of cellular heparan sulfate proteoglycan and a depletion of this molecule in the extracellular matrix. Concomitant with the decrease in the extracellular matrix is the appearance of a fraction of proteoglycan bearing altered carbohydrate moieties in the culture medium. beta-mercaptoethanol, mannitol, and dimethyl sulfoxide bring back to normal the structural properties of the proteoglycan in the medium and restore the matrix content in proteoglycan to a level comparable to that of control cells but do not affect the increase in the amount of proteoglycan on the cell. This "uncoupling" suggests that two independent chains of events underlie the synthetic and structural changes of the proteoglycan triggered by endotoxin in the endothelial cell.


J Cell Physiol


Colburn P,Kobayashi E,Buonassisi V




Has Abstract


1994-04-01 00:00:00












  • Presence of MUC4 in human milk and at the luminal surfaces of blood vessels.

    abstract::MUC4 is a heterodimeric membrane mucin, composed of a mucin subunit ASGP-1 (MUC4alpha) and a transmembrane subunit ASGP-2 (MUC4beta), which has been implicated in the protection of epithelial cell surfaces. Surprisingly, development and characterization of a new monoclonal antibody (mAb), called 1G8, against ASGP-2 de...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang J,Perez A,Yasin M,Soto P,Rong M,Theodoropoulos G,Carothers Carraway CA,Carraway KL

    更新日期:2005-07-01 00:00:00

  • Effects of nutritional and hormonal factors on the metabolism of retinol-binding protein by primary cultures of rat hepatocytes.

    abstract::Studies were conducted to explore hormonal and nutritional factors that might be involved in the regulation of retinol-binding protein (RBP) synthesis and secretion by the liver. The studies employed primary cultures of hepatocytes from normal rats. When cells were cultured in Dulbecco's modified Eagle's medium alone,...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dixon JL,Goodman DS

    更新日期:1987-01-01 00:00:00

  • Cdk5 mediates vimentin Ser56 phosphorylation during GTP-induced secretion by neutrophils.

    abstract::Secretion by neutrophils contributes to acute inflammation following injury or infection. Vimentin has been shown to be important for secretion by neutrophils but little is known about its dynamics during secretion, which is regulated by cyclin-dependent kinase 5 (Cdk5). In this study, we sought to examine the vimenti...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lee KY,Liu L,Jin Y,Fu SB,Rosales JL

    更新日期:2012-02-01 00:00:00

  • The long noncoding RNA ZFAS1 promotes the progression of glioma by regulating the miR-150-5p/PLP2 axis.

    abstract::Numerous studies have reported that long noncoding RNA (lncRNA) dysregulation is involved in the progression of many malignant tumors, including glioma. The lncRNA ZNFX1 antisense RNA 1 (ZFAS1) plays an oncogenic role in various malignant tumors, such as gastric cancer and hepatocellular carcinoma. However, the underl...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Li X,Luo Y,Liu L,Cui S,Chen W,Zeng A,Shi Y,Luo L

    更新日期:2020-03-01 00:00:00

  • Suppression of Glutamine:fructose-6-phosphate amidotransferase-1 inhibits adipogenesis in 3T3-L1 adipocytes.

    abstract::O-linked N-acetylglucosamine (O-GlcNAc) protein modification has been implicated in the regulation of signaling pathways, cell function, and gene expression. Glutamine:fructose-6-phosphate amidotransferase-1 (GFAT-1) is the rate-limiting enzyme in the hexosamine biosynthetic pathway (HBP), which generates the sugar nu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hsieh TJ,Lin T,Hsieh PC,Liao MC,Shin SJ

    更新日期:2012-01-01 00:00:00

  • Long noncoding RNA MT1JP inhibits proliferation, invasion, and migration while promoting apoptosis of glioma cells through the activation of PTEN/Akt signaling pathway.

    abstract::This study is carried out to elucidate the role of long noncoding RNAs (lncRNAs) MT1JP in proliferation, invasion, migration, and apoptosis of glioma cells through the regulation of PTEN/Akt signaling pathway. The expression of MT1JP in 80 normal brain tissues and 138 glioma tissues, as well as glioma cell lines, was ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang Y,Sui R,Chen Y,Liang H,Shi J,Piao H

    更新日期:2019-11-01 00:00:00

  • Primary colonospheres maintain stem cell-like key features after cryopreservation.

    abstract::The development of efficient and repeatable protocols for biobanking and prolonged storage of cancer stem cells (CSCs), with minimum alterations in biological function, is valuable and desired, particularly for retrospective analysis and clinical applications. In particular, data regarding the effect of cryopreservatio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Eini L,Naseri M,Karimi-Busheri F,Bozorgmehr M,Ghods R,Madjd Z

    更新日期:2020-03-01 00:00:00

  • Variations in the cell-free translating apparatus of cultured animal cells as a function of time during cell growth.

    abstract::Vero M3 cells, a line derived from the kidney of an African Green Monkey, display certain alterations in their protein synthetic apparatus as a function of time during a growth cycle. (Growth cycle here refers to exponential growth of unsynchronized cells in culture and their subsequent passage into the stationary pha...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Engelhardt DL,Sarnoski J

    更新日期:1975-08-01 00:00:00

  • The circ_0021977/miR-10b-5p/P21 and P53 regulatory axis suppresses proliferation, migration, and invasion in colorectal cancer.

    abstract::An in-depth understanding of circular RNAs (circRNAs) indicates that they are abundant in the eukaryotic transcriptome. Many circRNAs act as microRNA sponges; thus, they represent a new type of regulatory factor. However, the role of circRNA in colorectal cancer (CRC) remains largely unknown. Low circ_0021977 expressi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lu C,Jiang W,Hui B,Rong D,Fu K,Dong C,Tang W,Cao H

    更新日期:2020-03-01 00:00:00

  • Thermotolerance induced at a mild temperature of 40 degrees C protects cells against heat shock-induced apoptosis.

    abstract::Apoptosis constitutes a response of organisms to various physiological or pathological stimuli, and to different stresses. The ability of thermotolerance induced at a mild temperature of 40 degrees C to protect against activation of the apoptotic cascade by heat shock was investigated. When Chinese hamster ovary and h...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bettaieb A,Averill-Bates DA

    更新日期:2005-10-01 00:00:00

  • Replication kinetics of three DNA sequence families in synchronized mouse cells.

    abstract::Balb/C3T3 cells entered the quiescent Go state following serum deprivation. On addition of fresh serum, more than 95% of the culture resumed growth, but with asynchronous kinetics. If hydroxyurea were added just before the first cells reached S phase, at at least 90% of the cells accumulated at the Gl/S border over th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dooley DC,Ozer HL

    更新日期:1977-02-01 00:00:00

  • The relationship between purines, pyrimidines, nucleosides, and glutamine for fibroblast cell proliferation.

    abstract::Previous studies indicate that glutamine is a critical requirement for cell proliferation in vitro. We recently showed that depletion of glutamine from the culture medium supporting growing cells significantly reduced the proportion of cells undergoing DNA synthesis. Similarly glutamine depletion significantly reduced...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Engström W,Zetterberg A

    更新日期:1984-08-01 00:00:00

  • LINC00520 targeting miR-27b-3p regulates OSMR expression level to promote acute kidney injury development through the PI3K/AKT signaling pathway.

    abstract:BACKGROUND:Acute kidney injury (AKI) shows several kinds of disorders, which acutely harm the kidney. However, the current medical methods have limited therapeutic effects. The present study aimed to find out the molecular mechanism of AKI pathogenesis, which may provide new insights for future therapy. METHODS:Bioinf...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tian X,Ji Y,Liang Y,Zhang J,Guan L,Wang C

    更新日期:2019-08-01 00:00:00

  • Branched-chain amino acids inhibit proteolysis in rat skeletal muscle: mechanisms involved.

    abstract::Incubation of isolated rat soleus and EDL muscles in the presence of 10 mM leucine resulted in a decreased proteolytic rate as measured by the release of tyrosine into the incubation medium. The effect of this branched-chain amino acid (BCAA) is associated with a decreased activity of the lysosomal proteases and a dec...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Busquets S,Alvarez B,Llovera M,Agell N,López-Soriano FJ,Argilés JM

    更新日期:2000-09-01 00:00:00

  • The impact of culture dimensionality on behavioral epigenetic memory contributing to pluripotent state of iPS cells.

    abstract::Three-dimensional (3D) culture platforms have been explored to establish physiologically relevant cell culture environment and permit expansion scalability; however, little is known about the mechanisms underlying the regulation of pluripotency of human induced pluripotent stem cells (hiPSCs). This study elucidated ep...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Thanuthanakhun N,Kino-Oka M,Borwornpinyo S,Ito Y,Kim MH

    更新日期:2020-12-10 00:00:00

  • Expression of parathyroid hormone-related protein in cells of osteoblast lineage.

    abstract::The expression of parathyroid hormone-related protein (PTHrP) was studied in a range of cell cultures representative of the osteoblast lineage and in rat calvarial sections. Primary newborn rat calvarial cells, a rat preosteoblastic cell line (UMR 201), a mouse stromal cell line (ST 2), a mouse calvaria-derived osteob...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Suda N,Gillespie MT,Traianedes K,Zhou H,Ho PW,Hards DK,Allan EH,Martin TJ,Moseley JM

    更新日期:1996-01-01 00:00:00

  • Stem cell factor can overcome inhibition of highly purified human burst-forming units-erythroid by interferon gamma.

    abstract::Highly purified human blood burst-forming units-erythroid (BFU-E) were used to study the effects of interferon gamma (IFN gamma). IFN gamma inhibited erythroid colony formation, cell proliferation, and differentiation of day 3 to day 6 mature BFU-E in a dose-dependent manner. The primitive BFU-E (day 1 and day 2 cells...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dai CH,Krantz SB,Kollar K,Price JO

    更新日期:1995-11-01 00:00:00

  • Protective effects of irisin on hypoxia-reoxygenation injury in hyperglycemia-treated cardiomyocytes: Role of AMPK pathway and mitochondrial protection.

    abstract::Recent evidence has verified the cardioprotective actions of irisin in different diseases models. However, the beneficial action of irisin on hypoxia-reoxygenation (HR) injury under high glucose stress has not been described. Herein our research investigated the influence of irisin on HR-triggered cardiomyocyte death ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Fan J,Zhu Q,Wu Z,Ding J,Qin S,Liu H,Miao P

    更新日期:2020-02-01 00:00:00

  • Separation of isolated hamster intestinal epithelial cells by velocity sedimentation on Ficoll Gradients.

    abstract::Isolated hamster intestinal epithelial cells can be separated by velocity sedimentationion on 2-10% Ficoll gradients into three subpopulations of cells which differ in morphology, biochemistry, physiology, and membrane components. These subpopulations are not pure but are enriched in a single cell type to the extent t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Carter JH,Poretz RD,Eichholz A

    更新日期:1982-04-01 00:00:00

  • Changes in luminal flow rate modulate basal and bradykinin-stimulated cell [Ca2+] in aortic endothelium.

    abstract::Hemodynamic forces influence many endothelial cell functions. The coupling between hemodynamic forces and cell function could be mediated by mechano-sensitive ion channels present in the plasma membrane of endothelial cells. Because one of these channels is permeable to Ca2+, we tested whether hemodynamic forces influ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Oliver JA,Chase HS Jr

    更新日期:1992-04-01 00:00:00

  • Adipose-derived stem cells and cancer cells fuse to generate cancer stem cell-like cells with increased tumorigenicity.

    abstract::Adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cells isolated from adipose tissue and have the ability to differentiate into adipogenic, osteogenic, and chondrogenic lineages. Despite their great therapeutic potentials, previous studies showed that ADSCs could enhance the proliferation and metastati...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chan YW,So C,Yau KL,Chiu KC,Wang X,Chan FL,Tsang SY

    更新日期:2020-10-01 00:00:00

  • LncRNA NKILA inhibits the proliferation and promotes the apoptosis of CSCC cells by downregulating miRNA-21.

    abstract::Long noncoding RNA (lncRNA) NKILA has been well studied in several types of human tumors as a tumor suppressor, while its involvement in cervical squamous cell carcinoma (CSCC) remains unclear. In our studies, we found that serum NKILA was at lower levels and serum microRNA-21 (miRNA-21) was at higher levels in patien...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wang J,Zhu Z,Qiu H,Liu C,Chang X,Qi Y,Lv Q

    更新日期:2020-11-01 00:00:00

  • Plasma membrane fluidity measurements in intact endothelial cells: effect of hyperoxia on fluorescence anisotropies of 1-[4-(trimethylamino)phenyl]-6-phenyl hexa-1,3,5-triene.

    abstract::Fluorescence anisotropy measurements are widely used as sensitive indicators of cell membrane fluidity. 1-[4-(trimethylamino)phenyl]-6-phenyl hexa-1,3,5-triene (TMA-DPH) is a cationic fluorescent aromatic hydrocarbon that anchors at the lipid-water interface of membrane lipid bilayers. Its uptake into porcine pulmonar...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sheridan NP,Block ER

    更新日期:1988-01-01 00:00:00

  • Chemically and virally transformed cells able to grow without anchorage in serum-free medium: evidence for an autocrine growth factor.

    abstract::BA10-IR transformed cells, obtained by treating Syrian hamster embryo fibroblasts (HEF) with 7-methylbenz(a)anthracene and cultivated for a long period, are highly tumorigenic and grow in suspension as aggregates (spheroids) (Levy et al., 1976). They also grow in attached form or as spheroids in serum-free (S-) synthe...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xin LW,Jullien P,Lawrence DA,Pironin M,Vigier P

    更新日期:1987-05-01 00:00:00

  • Mouse teratocarcinoma cells resistant to aphidicolin and arabinofuranosyl cytosine: isolation and initial characterization.

    abstract::With the long-range goals of elucidating the biochemical genetics and the phenotypic expression, both in vitro and in vivo, of mutator strains of mammalian cells, we have isolated and partially characterized a series of drug-resistant mutants from a subline a feeder-dependent parental mouse teratocarcinoma line (PSA-1...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Aizawa S,Loeb LA,Martin GM

    更新日期:1983-04-01 00:00:00

  • Dominant and recessive mitogen-nonproliferative variants of 3T3 cells.

    abstract::We have previously isolated 3T3 cell variants unable to respond to specific mitogens. In this report we analyze the dominant and/or recessive nature of these variants. Two independently isolated EGF nonproliferative variants are unable to bind EGF. Hybrids between 3T3R5 cells (thymidine kinase deficient, ouabain-resis...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Terwilliger E,Herschman HR

    更新日期:1985-06-01 00:00:00

  • Purine and phosphoribosylpyrophosphate synthesis in differentiating murine virus-induced erythroleukemic cells in vitro.

    abstract::Phosphoribosylpyrophosphate synthetase activity was determined in Friend virus-inducted erythroleukemic cells in culture, stimulated to differentiate in the presence of dimethylsulfoxide. The activity of phosphoribosylpyrophosphate synthetase did not decrease in cells which had acquired the specialized function of hem...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Reem GH,Friend C

    更新日期:1976-06-01 00:00:00

  • Possible involvement of p21 but not of p16 or p53 in keratinocyte senescence.

    abstract::It has been reported that p21, p53, and p16 affect the cell cycle and cell senescence. However, their roles in keratinocyte senescence are not clear. We established primary keratinocyte strains from 15 donors and maintained them until replicative senescence; their population doublings ranged from 5.7-45.2. These strai...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sayama K,Shirakata Y,Midorikawa K,Hanakawa Y,Hashimoto K

    更新日期:1999-04-01 00:00:00

  • Novel hedgehog agonists promote osteoblast differentiation in mesenchymal stem cells.

    abstract::Hedgehog (Hh) family members are involved in multiple cellular processes including proliferation, migration, differentiation, and cell fate determination. Recently, the novel Hh agonists Hh-Ag 1.3 and 1.7 were identified in a high-throughput screening of small molecule compounds that activate the expression of Gli1, a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Nakamura T,Naruse M,Chiba Y,Komori T,Sasaki K,Iwamoto M,Fukumoto S

    更新日期:2015-04-01 00:00:00

  • High expression of IMPACT protein promotes resistance to indoleamine 2,3-dioxygenase-induced cell death.

    abstract::Indoleamine 2,3-dioxygenase (IDO), a tryptophan degrading enzyme, is a potent immunomodulatory factor. IDO expression in fibroblasts selectively induces apoptosis in immune cells but not in primary skin cells. However, the mechanism(s) of this selective effect of IDO-induced low tryptophan environment is not elucidate...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Habibi D,Jalili RB,Forouzandeh F,Ong CJ,Ghahary A

    更新日期:2010-10-01 00:00:00