Abstract:
:Human skin fibroblasts contracted fibrin gels in a time- and cell-dependent manner. Under optimal conditions, gel contraction amounted to more than 50% in 2 hr. Fibronectin did not promote contraction, and fibrinolysis was not required for contraction, although gels contracted without serum or aprotinin were lysed. Before contraction, fibrin was present in loosely packed, randomly organized fibrils. After contraction, the fibrils were more densely packed and aligned in the plane of cell spreading. Cycloheximide treatment of fibroblasts inhibited gel contraction in serum-free medium but not in serum-containing medium. Fibronectin could not substitute for serum in overcoming the cycloheximide effect. Binding sites for fibrin were distributed randomly over the cells' surfaces based on electron microscopic observations. Often small groups of fibrils were localized in indentations at the cell surface. Finally, peptides containing the arg-gly-asp-ser sequence inhibited gel contraction.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Tuan TL,Grinnell Fdoi
10.1002/jcp.1041400324subject
Has Abstractpub_date
1989-09-01 00:00:00pages
577-83issue
3eissn
0021-9541issn
1097-4652journal_volume
140pub_type
杂志文章abstract::Melanoma as the most major skin malignancy has attracted much attention, so far. Although a successful therapeutic strategy requires an accurate understanding of the precise mechanisms for the initiation and progression of the melanoma. Several types of cell death mechanisms have recently been identified along with co...
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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abstract::Breast cancer (BC) is the most prevalent malignant cancer in the world, is the leading cause of cancer-related death female. Recently, there is accumulating evidence that long noncoding RNAs (lncRNAs) might as an important role in the progression of BC. (epithelial-mesenchymal transition (EMT) is considered to play a ...
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journal_title:Journal of cellular physiology
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更新日期:2016-04-01 00:00:00
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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doi:10.1002/jcp.1041050321
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journal_title:Journal of cellular physiology
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更新日期:1998-02-01 00:00:00