Abstract:
:The Na+ pump (Na+, K+-ATPase) has been implicated in the regulation of many cellular functions, including cell volume regulation. The effects of inhibiting Na+ pump activity on cell volume and taurine efflux were evaluated in the human neuroblastoma cell line CHP-100. Cell volume changes monitored with the Coulter Multisizer technique and confocal microscopy showed that neuroblastoma cells exposed to ouabain swelled by 22 +/- 4% (n = 5). The rapid cell swelling was followed by regulatory volume decrease (RVD). In cells treated with ouabain, 14C-taurine efflux increased by 183 +/- 11% compared with controls. However, cells exposed simultaneously to ouabain and hypoosmotic solution resulted in a 14C-taurine efflux of 207 +/- 18%. Western blot and immunofluorescence microscopy with specific monoclonal antibodies for the catalytic alpha isoforms of Na+, K+-ATPase demonstrated high levels of the ubiquitously expressed alpha1 and the neuronal-specific alpha3. Ouabain-binding data showed that CHP-100 cells express approximately 3 x 10(5) pump units/cell. The present data indicate that efflux of taurine may be involved during volume recovery subsequent to blockade of Na+, K+-ATPase in CHP-100 cells.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Basavappa S,Mobasheri A,Errington R,Huang CC,Al-Adawi S,Ellory JCdoi
10.1002/(SICI)1097-4652(199802)174:2<145::AID-JCP1subject
Has Abstractpub_date
1998-02-01 00:00:00pages
145-53issue
2eissn
0021-9541issn
1097-4652pii
10.1002/(SICI)1097-4652(199802)174:2<145::AID-JCP1journal_volume
174pub_type
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