Abstract:
:For malignant cells cultured from a human astrocytoma, electrophysiological characteristics of the plasma membrane included specific resistivity of 446.82 +/- 279.5 ohm . cm2, specific capacitance of 0.758 +/- 0.52 microfarads/cm2, time constant 0.318 +/- 0.10 msec. The resting membrane potential averaged--14.07 +/- 7.4 mV; the mean input resistance 8.1 +/- 4.0 megohms. The average cell area was 1638 +/- 585 mu2 for contactual and and 1919 +/- 989 mu2 for noncontactual cells. Changes in input resistance and resting membrane potential were observed with increasing time in culture, possibly reflecting cel cycling. There did not appear to be electrical coupling in this cell line.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Black PM,Kornblith PLdoi
10.1002/jcp.1041050321subject
Has Abstractpub_date
1980-12-01 00:00:00pages
565-70issue
3eissn
0021-9541issn
1097-4652journal_volume
105pub_type
杂志文章abstract::Mitogen-activated protein (MAP) kinases are serine/threonine kinases that are rapidly activated in response to mitogenic stimuli. Here we examined the enzymatic activity and phosphorylation state of the individual p44mapk and p42mapk isoforms during early G1 and late G1 phase of the mammalian cell cycle. Release of fi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041630319
更新日期:1995-06-01 00:00:00
abstract::The bone marrow landscape consists of specialized and stem/progenitor cells, which coordinate important tissue-related and systemic physiological features. Within the marrow cavity, stem/progenitor and differentiated hematopoietic and skeletal cells congregate into dynamic functional assemblies throughout specific ana...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30296
更新日期:2021-01-25 00:00:00
abstract::Galectin-1 is reported to be upregulated in various human cancers. However, the relationship between galectin-1 expression and cancer prognosis has not been systematically assessed. In this study, we searched PubMed, Web of Science, and Embase to collect all relevant studies and a meta-analysis was performed. We found...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,meta分析
doi:10.1002/jcp.28059
更新日期:2019-08-01 00:00:00
abstract::The human polyomavirus, JC virus (JCV), provides an excellent model system to investigate the reciprocal interaction of the immune and nervous systems. Infection with JCV occurs during childhood and the virus remains in the latent state with no apparent clinical symptoms. However, under immunosuppressed conditions, th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.10096
更新日期:2002-06-01 00:00:00
abstract::The development of acute pancreatitis (AP) is triggered by acinar events, but the subsequent extra-acinar events, particularly a distinct immune response, appear to determine its severity. Cytokines modulate this immune response and are derived not only from immunocytes but also from pancreatic acinar cells. We studie...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20216
更新日期:2005-04-01 00:00:00
abstract::Although the mechanism is unknown, infiltration anesthetics are believed to have membrane-stabilizing action. We report here that such a most commonly used anesthetic, lidocaine, effectively inhibited the invasive ability of human cancer (HT1080, HOS, and RPMI-7951) cells at concentrations used in surgical operations ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10145
更新日期:2002-09-01 00:00:00
abstract::Long noncoding RNAs (lncRNAs) have been shown to have critical regulatory roles in tumorigenesis. lncRNA LINC01561 (LINC01561) is a newly identified tumor-related lncRNA and its dysregulation has been demonstrated in several tumors. However, whether LINC01561 is involved in the progression of non-small-cell lung carci...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29564
更新日期:2020-10-01 00:00:00
abstract::Circular RNA TCF25 (circTCF25) has been proven to be upregulated in human malignancy and correlated with tumor process. Our study aimed to unravel whether circTCF25 dictates cellular activities of osteosarcoma cells and address the possible mechanisms associated with microRNA (miR). circTCF25 and miR-206 in clinical s...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29570
更新日期:2020-02-13 00:00:00
abstract::Bafilomycin A1, a specific inhibitor of H(+)-ATPases of the vacuolar type, was in the present study shown, at similar concentrations, to induce secretion of lysosomal enzyme and to elevate lysosomal pH in mouse macrophages. These results lend support to the previous suggestion of a triggering role for an increase in l...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041630116
更新日期:1995-04-01 00:00:00
abstract::We have examined the effects of constitutive expression of PTHrP on the growth and differentiation of populations of cells derived from a clonal chondrocytic cell line, CFK2. Cells were stably transfected with cDNA encoding either full-length, secretory PTHrP (CFK2P) or nonsecretory PTHrP (CFK2P-SS). In cultures of ce...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199610)169:1<33::AID-JCP4>
更新日期:1996-10-01 00:00:00
abstract::Osteoclasts mediate bone resorption by secretion at the site of bone attachment. This process depends on calmodulin concentrated at a specialized acid-secreting membrane. We hypothesized that increased calmodulin and bone attachment were required for acid secretion. We tested this by studying calmodulin, bone attachme...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199612)169:3<411::AID-JCP1
更新日期:1996-12-01 00:00:00
abstract::Despite the advantages of transplantation of umbilical cord blood's (UCB's) hematopoietic stem cells (uHSCs) for hematologic malignancy treatment, there are two major challenges in using them: (a) Insufficient amount of uHSCs in a UCB unit; (b) a defect in uHSCs homing to bone marrow (BM) due to loose binding of their...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28825
更新日期:2019-12-01 00:00:00
abstract::Spiral artery remodeling at the maternal-fetal interface is crucial for successful pregnancy and requires the interaction between the first trimester trophoblast and the endothelial cells of the maternal vessels. However, the precise mechanism of this dialog has yet to be determined. The current study investigated whe...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27358
更新日期:2019-05-01 00:00:00
abstract:AIM:We investigated melanoma-associated antigen A1 (MAGE-A1) expression in lung cancer tissues and its correlation with prognostic factors. MATERIALS AND METHODS:In this cross-sectional study, samples from 101 patients with lung cancer were obtained between 2007 and 2014 and stained for MAGE-A1 by immunohistochemistry...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27884
更新日期:2019-07-01 00:00:00
abstract::Amylin is a 37 amino acid peptide produced mainly by beta-cells of the endocrine pancreas. Human amylin has 43% homology with human calcitonin gene-related peptide (CGRP) and 13% homology with human calcitonin (CT). Amylin and CGRP have been reported to have CT-like hypocalcemic activity in vivo. To investigate the ro...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041530103
更新日期:1992-10-01 00:00:00
abstract::The effect of cell culture age and concomitant changes in cell density on the biosynthesis of sulfated-proteoglycan by rabbit articular chondrocytes in secondary monolayer culture was studied. Low density (LD, 2 d), middle density (MD, 5-7 d), and high density (HD, 12-15 d) cultures demonstrated changes in cellular mo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041210315
更新日期:1984-12-01 00:00:00
abstract::Programmed cell death in mammary tissue was studied during natural weaning in lactating mice and after litter removal or milk stasis. All treatments stimulated mammary apoptosis, indicating that this process is an integral part of the tissue's involution after lactation. Induction of apoptosis was slower in natural we...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199609)168:3<559::AID-JCP8
更新日期:1996-09-01 00:00:00
abstract::Ion channels are pivotal to many aspects of sperm physiology and function. We have used the patch clamp technique to investigate the distribution of ion channels in the plasma membrane of the head of human spermatozoa. We report that three types of activity are common in the equatorial and acrosomal regions of the spe...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21153
更新日期:2007-12-01 00:00:00
abstract::Previous study suggested that the receptor component protein (RCP), one of the components of calcitonin gene-related peptide (CGRP) receptor, plays a multiple role in the cellular signal transduction. The study was designed to investigate whether or not the RCP involved in the regulation of caveolin-1/extracellular si...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26582
更新日期:2018-10-01 00:00:00
abstract::Methylxantine derivative, caffeine, is known to prevent the p53-dependent apoptosis pathway via inhibition of ATM (ataxia telangiectasia mutated) kinase, which activates p53 by phosphorylation of the Ser-15 residue. In contrast, it has been reported that caffeine induces p53-mediated apoptosis through Bax protein in n...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10289
更新日期:2003-08-01 00:00:00
abstract::We examined the expression of the estrogen and epidermal growth factor (EGF) receptors in a drug-resistant subline of MCF-7 cells in order to study potential alterations in hormone dependence or in the growth factor pathway that could be related to the development of drug resistance in human breast cancer. The drug-re...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041570115
更新日期:1993-10-01 00:00:00
abstract::B cell activating factor from the TNF family (BAFF) stimulates B-cell proliferation and survival, but excessive BAFF promotes the development of aggressive B cells leading to malignant and autoimmune diseases. Recently, we have reported that rapamycin, a macrocyclic lactone, attenuates human soluble BAFF (hsBAFF)-stim...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25913
更新日期:2018-01-01 00:00:00
abstract::The development of resistance accounts for therapy failure in the majority of advanced cases of neuroblastoma in children. A new transplantable murine C-1300 neuroblastoma cell line was developed in vitro, by repeated exposure of a sensitive cell line to increasing, but sublethal, doses of Homoharringtonine (HHT). The...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041480319
更新日期:1991-09-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is mainly associated with hepatitis B virus (HBV) infection and characterized by metastasizing and infiltrating adjacent and distant tissues. Notably, microRNA-1271 (miR-1271) is a tumor suppressor in various cancers. Therefore, we evaluate the ability of miR-1271 to influence cell proli...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26955
更新日期:2019-04-01 00:00:00
abstract::Nitric oxide triggers cGMP-dependent kinase-mediated phosphorylation of the actin regulator vasodilator-stimulated phosphoprotein (VASP) at residue serine239. The function of this phosphorylation for smooth muscle cell (SMC) adhesion, spreading, matrix contraction, and invasion is not well understood. We reconstituted...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21942
更新日期:2010-01-01 00:00:00
abstract::3-Hydroxy 3-methylglutaryl coenzyme A reductase (HMG-CoAR) is the key and rate-limiting enzyme of cholesterol biosynthetic pathway. Although HMG-CoAR activity has already been related to the differentiation of some cellular lines there are no studies that analyze the role of HMG-CoAR, and the pathway it is involved wi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21810
更新日期:2009-08-01 00:00:00
abstract::Macrophages and many chemokines are closely associated with the adipogenic differentiation of bone marrow mesenchymal stem cells (MSCs), but their roles in adipogenesis and the underlying mechanisms are not fully understood. Here, we first investigated the influence of macrophages on the differentiation of MSCs in vit...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27634
更新日期:2019-06-01 00:00:00
abstract::The matricellular protein periostin is strongly expressed in collagen-rich connective tissues such as periodontal ligaments (PDLs), skeletal muscle, adipose tissue, tendons, skin, and bone. It is prominent in tumorigenesis, angiogenesis, and cardiac repair. It is localized in the periosteum and PDL, where it is seen i...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.24407
更新日期:2014-01-01 00:00:00
abstract::Minute virus of mice (MVM), a non-defective parvovirus, has been shown to infect cultures of non-pluripotent differentiated teratocarcinoma-derived cells, but pluripotent (and "nullipotent") embryonal carcinoma cells derived from the same teratocarcinoma resist MVN infection. Somatic cell hybrids between an embryonal ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040910309
更新日期:1977-06-01 00:00:00
abstract::Previously, serum miR-188-5p is differentially expressed in breast cancer, but the diagnostic potential of circulating miR-188-5p as well as its regulatory mechanism in breast cancer remain uncertain. Herein, serum miR-188-5p was detected by real-time polymerase chain reaction in patients with breast cancer, breast fi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29367
更新日期:2020-05-01 00:00:00