Abstract:
:Bafilomycin A1, a specific inhibitor of H(+)-ATPases of the vacuolar type, was in the present study shown, at similar concentrations, to induce secretion of lysosomal enzyme and to elevate lysosomal pH in mouse macrophages. These results lend support to the previous suggestion of a triggering role for an increase in lysosomal pH and a permissive role for cytosolic pH in the exocytosis of macrophage lysosomal enzyme. Vacuolar H(+)-ATPases are present in the macrophage plasma membrane as well as in intracellular membranes, for example, those of the lysosomal and phagosomal compartments. Phagosomal acidification was shown to be achieved in part by a mechanism with a similar sensitivity to bafilomycin A1 as lysosomal H+ transport and in part by an early, bafilomycin A1-insensitive mechanism. We found a lesser sensitivity towards bafilomycin A1 of the lysosomal and phagosomal H(+)-ATPase than that localized in the plasma membrane, indicating differences among H(+)-ATPases at the subcellular level. Also, by attempts to mobilize lysosomal H(+)-ATPase to the plasma membrane, support was obtained for the notion that subcellular H(+)-ATPase populations differ and thus possibly could be differentially regulated.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Tapper H,Sundler Rdoi
10.1002/jcp.1041630116subject
Has Abstractpub_date
1995-04-01 00:00:00pages
137-44issue
1eissn
0021-9541issn
1097-4652journal_volume
163pub_type
杂志文章abstract::A quantitative assay for intercellular adhesion, which is a modification of the collecting aggregate assay (Roth et al., '71) is described. The use of mechanically dissociated single cells labeled with 3H-leucine, and of a gyratory shaker increased considerably the efficiency of cell collection. With chick embryo neur...
journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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abstract::Non-small-cell lung cancer (NSCLC) remains the leading cause of cancer death worldwide. As a platinum-based chemotherapeutic drug, cisplatin has been used for over 30 years in NSCLC treatment while its effects are diminished by drug resistance. Therefore, we aimed to study the potential role of UCA1 in the development...
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更新日期:1985-03-01 00:00:00
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更新日期:2013-11-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2018-09-01 00:00:00
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更新日期:2013-02-01 00:00:00
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journal_title:Journal of cellular physiology
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doi:10.1002/jcp.1041490307
更新日期:1991-12-01 00:00:00
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更新日期:2004-02-01 00:00:00
abstract::These are the reflections of a retired physician who was introduced to cellular physiology in 1947 at the University of Pennsylvania. I majored in Zoology. I took the required major courses, which were interesting but not exciting. I was a premedical student at the time. Fortunately I was introduced to cellular physio...
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journal_title:Journal of cellular physiology
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更新日期:1983-02-01 00:00:00
abstract::Modeling breast cancer in the mouse has helped to better define the heterogeneity of human breast cancer. In the recent past, it has become evident that some limitations have restricted the potential benefits that can be achieved with this approach. In this review, we highlight some key points that should be taken int...
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更新日期:2012-08-01 00:00:00
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更新日期:1993-01-01 00:00:00