Abstract:
:We examined the behavior of the transcription factor Max during retrograde neuronal degeneration of retinal ganglion cells. Using immunohistochemistry, we found a progressive redistribution of full-length Max from the nucleus to the cytoplasm and dendrites of the ganglion cells following axon damage. Then, the axotomized cells lose all their content of Max, while undergoing nuclear pyknosis and apoptotic cell death. After treatment of retinal explants with either anisomycin or thapsigargin, the rate of nuclear exclusion of Max accompanied the rate of cell death as modulated by either drug. Treatment with a pan-caspase inhibitor abolished both TUNEL staining and immunoreactivity for activated caspase-3, but did not affect the subcellular redistribution of Max immunoreactivity after axotomy. The data show that nuclear exclusion of the transcription factor Max is an early event, which precedes and is independent of the activation of caspases, during apoptotic cell death in the central nervous system.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Petrs-Silva H,de Freitas FG,Linden R,Chiarini LBdoi
10.1002/jcp.10404keywords:
subject
Has Abstractpub_date
2004-02-01 00:00:00pages
179-87issue
2eissn
0021-9541issn
1097-4652journal_volume
198pub_type
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