Abstract:
:Retinoic acid (RA) reduced growth, fibronectin, and retinoic acid receptor (RAR alpha) in NIH 3T3 cells but not in cells transformed by the Ha-ras oncogene. RA lowered RAR alpha transcript and protein, increased RAR beta transcripts, and had no effect on RAR gamma. H-ras transformation downregulated RAR expression and abolished responsiveness to RA. Ha-ras-transformed cells were as active as normal NIH-3T3 cells in RA uptake but were unable to degrade it to medium oxidation product, so that, paradoxically, the resistant cells accumulated 20-30-fold as much RA as the sensitive cells. RA sensitivity/insensitivity correlated with RA metabolism/lack thereof in 15 cell lines in serum-free medium. These data suggest a relationship between RA inhibition of cell growth and intracellular RA metabolism.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
De Luca LM,Scita G,Takatsuka Jdoi
10.1002/(SICI)1097-4652(199711)173:2<297::AID-JCP3subject
Has Abstractpub_date
1997-11-01 00:00:00pages
297-300issue
2eissn
0021-9541issn
1097-4652pii
10.1002/(SICI)1097-4652(199711)173:2<297::AID-JCP3journal_volume
173pub_type
杂志文章abstract::Phosphoribosylpyrophosphate synthetase activity was determined in Friend virus-inducted erythroleukemic cells in culture, stimulated to differentiate in the presence of dimethylsulfoxide. The activity of phosphoribosylpyrophosphate synthetase did not decrease in cells which had acquired the specialized function of hem...
journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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更新日期:2007-04-01 00:00:00
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更新日期:1980-03-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:1989-01-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:1998-01-01 00:00:00
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更新日期:1988-12-01 00:00:00
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更新日期:2018-04-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041110311
更新日期:1982-06-01 00:00:00
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