Abstract:
:Intratympanic injection of gentamicin has proven to be an effective therapy for intractable vestibular dysfunction. However, most studies to date have focused on the cochlea, so little is known about the distribution and uptake of gentamicin by the counterpart of the auditory system, specifically vestibular hair cells (HCs). Here, with a combination of in vivo and in vitro approaches, we used a gentamicin-Texas Red (GTTR) conjugate to investigate the mechanisms of gentamicin vestibulotoxicity in the developing mammalian utricular HCs. In vivo, GTTR fluorescence was concentrated in the apical cytoplasm and the cellular membrane of neonatal utricular HCs, but scarce in the nucleus of HCs and supporting cells. Quantitative analysis showed the GTTR uptake by striolar HCs was significantly higher than that in the extrastriola. In addition, the GTTR fluorescence intensity in the striola was increased gradually from 1 to 8 days, peaking at 8-9 days postnatally. In vitro, utricle explants were incubated with GTTR and candidate uptake conduits, including mechanotransduction (MET) channels and endocytosis in the HC, were inhibited separately. GTTR uptake by HCs could be inhibited by quinine, a blocker of MET channels, under both normal and stressed conditions. Meanwhile, endocytic inhibition only reduced GTTR uptake in the CoCl2 hypoxia model. In sum, the maturation of MET channels mediated uptake of GTTR into vestibular HCs. Under stressed conditions, MET channels play a pronounced role, manifested by channel-dependent stress enhanced GTTR permeation, while endocytosis participates in GTTR entry in a more selective manner.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Qian X,He Z,Wang Y,Chen B,Hetrick A,Dai C,Chi F,Li H,Ren Ddoi
10.1002/jcp.30228subject
Has Abstractpub_date
2020-12-23 00:00:00eissn
0021-9541issn
1097-4652pub_type
杂志文章abstract::Cardiac fibroblast (CF) differentiation to myofibroblasts expressing α-smooth muscle actin (α-SMA) plays a key role in cardiac fibrosis. Therefore, a study of the mechanism regulating α-SMA expression is a means to understanding the mechanism of fibroblast differentiation and cardiac fibrosis. Previous studies have sh...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27471
更新日期:2019-05-01 00:00:00
abstract::The role of hsp27 as an inhibitor of actin polymerization was considered in the context of the actin cytoskeleton and its relationship with focal adhesion formation. The aim of this study was to evaluate the potential effects of hsp27 on focal adhesion formation as a relevant biological consequence of actin stress fib...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199812)177:4<575::AID-JCP8
更新日期:1998-12-01 00:00:00
abstract::During interphase, the centrosome concentrates cell stress response molecules, including chaperones and proteasomes, into a proteolytic center. However, whether the centrosome functions as proteolytic center during mitosis is not known. In this study, cultured mammalian cells were treated with the proteasome inhibitor...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20335
更新日期:2005-09-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) Rev protein facilitates the nuclear export of viral mRNA containing the Rev response element (RRE). Although several host proteins co-operating with Rev in viral RNA export have been reported, little is known about the innate host defense factors that Rev overcomes to me...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21305
更新日期:2008-01-01 00:00:00
abstract::Pain can vary over the estrous cycle as a result of changes in estradiol concentration but the mechanism causing this variation is unclear. Because the thalamus is important in pain control, gene expression in the lateral thalamus (ventral posteromedial, ventral posterolateral, reticular thalamic nuclei) was screened ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25198
更新日期:2016-05-01 00:00:00
abstract::Spinal cord injury (SCI) is a public health problem in the world. The SCI usually triggers an excessive inflammatory response that brings about a secondary tissue wreck leading to further cellular and organ dysfunction. Hence, there is great potential of reducing inflammation for therapeutic strategies of SCI. In this...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28124
更新日期:2019-08-01 00:00:00
abstract::Quercetin has potentially beneficial effects on disease prevention, including cancer. An intriguing issue regarding the mechanisms of action of quercetin is the ability of this drug to modulate estrogen receptor (ER) activities. In a previous study, we demonstrated that quercetin elicited apoptosis through an ERα-depe...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22917
更新日期:2012-05-01 00:00:00
abstract::Human lymphocytes were equilibrated for 48 hours at 5-6 mM K+ ex over a range of temperatures between 0 and 37 degrees C, and at 5 degrees C over a range of external K+ levels between 0 and 32 mM. Cell K+ and Na+ contents are normal between 37 and 10 decrees. Below 10 degrees there is a critical thermal transition in ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041030113
更新日期:1980-04-01 00:00:00
abstract::Osteopontin (OPN) is a major non-collagenous bone matrix protein implicated in the regulation of cell function. Although OPN is rich in the cementum of the tooth, the significance of OPN in this tissue is not understood. Tooth root resorption is the most frequent complication of orthodontic tooth movement (TM). The ob...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21250
更新日期:2008-03-01 00:00:00
abstract::Cardiac fibrosis is a pathological remodeling response to myocardial infarction (MI) and impairs cardiac contractility. Long noncoding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is increased in patients with MI. However, the functions of MALAT1 in cardiac fibrosis have not been elucid...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27117
更新日期:2019-03-01 00:00:00
abstract::A separation procedure has been developed for mouse splenic T and B lymphocytes which is based on their differential agglutination by wheat germ agglutinin (WGA). In the presence of 50-100 micrograms/ml of WGA, multicellular aggregates are formed which are enriched in B cells. These aggregates can be separated from mo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040990111
更新日期:1979-04-01 00:00:00
abstract::B cell activating factor from the TNF family (BAFF) stimulates B-cell proliferation and survival, but excessive BAFF promotes the development of aggressive B cells leading to malignant and autoimmune diseases. Recently, we have reported that rapamycin, a macrocyclic lactone, attenuates human soluble BAFF (hsBAFF)-stim...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25913
更新日期:2018-01-01 00:00:00
abstract::The application of the phenomenon of premature chromosome condensation for cell cycle analysis in HeLa and CHO cells has been examined. Random populations of HeLa and CHO cells pulse labelled with H3-TdR were separately fused with mitotic HeLa cells using U.V. inactivated Sendai virus. The resulting prematurely conden...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040910113
更新日期:1977-04-01 00:00:00
abstract::Cementum, which shares common features with bone in terms of biochemical composition, is important for the homeostasis of periodontium during periodontitis and orthodontic treatment. Sirtuin 6 (SIRT6), as a member of the sirtuin family, plays key roles in the osteogenic differentiation of bone marrow mesenchymal stem ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27213
更新日期:2019-04-01 00:00:00
abstract::A rat pheochromocytoma cell line (PC12 cells) was used as a model to investigate the role of calmodulin and its multiple mRNAs in NGF-induced neuronal differentiation. The effect of NGF on the degree of differentiation was assayed using a simple differentiation scoring system. Significant increases in the differentiat...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041490309
更新日期:1991-12-01 00:00:00
abstract::Expression of plasminogen activator inhibitor type-1 (PAI-1), a member of the SERPIN gene family that functions to regulate the plasmin-based pericellular proteolytic cascade, is growth state-regulated in normal rat kidney (NRK) cells (Ryan and Higgins, 1990, J. Cell. Physiol., 155:376-384; Ryan et al., 1996, Biochem....
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199701)170:1<8::AID-JCP2>3
更新日期:1997-01-01 00:00:00
abstract::Parathyroid hormone-related protein (PTHrP) (107-139), in contrast to the N-terminal fragment PTHrP (1-36), has been shown to interact with the vascular endothelial growth factor (VEGF) system to modulate human osteoblast differentiation. In this study, we evaluated whether this interaction might affect human osteobla...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21547
更新日期:2008-12-01 00:00:00
abstract:OBJECTIVE:Disc degeneration is the common life-threatening disease characterized by flank pain. The gene expression of insulin-like growth factor binding protein 3 (IGFBP3) is increased in patients with disc degeneration, however, its mechanism is still unknown. This study aimed to investigate the influence of IGFBP3 g...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27613
更新日期:2019-06-01 00:00:00
abstract::Resistance to tamoxifen remains a prominent conundrum in the therapy of hormone-sensitive breast cancer. Also, the molecular underpinnings leading to tamoxifen resistance remain unclear. In the present study, we utilized the Gene Expression Omnibus database to identify that SOX11 might exert a pivotal function in conf...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29629
更新日期:2020-10-01 00:00:00
abstract::Programmed cell death in mammary tissue was studied during natural weaning in lactating mice and after litter removal or milk stasis. All treatments stimulated mammary apoptosis, indicating that this process is an integral part of the tissue's involution after lactation. Induction of apoptosis was slower in natural we...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199609)168:3<559::AID-JCP8
更新日期:1996-09-01 00:00:00
abstract::Myofibroblasts mediate normal wound healing and upon chronic activation can contribute to the development of pathological conditions including organ fibrosis and cancer. Myofibroblasts can develop from epithelial cells through an epithelial-mesenchymal transition (EMT) during which epithelial cells exhibit drastic mor...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24895
更新日期:2015-08-01 00:00:00
abstract::Cultured bovine adrenocortical cells were previously shown to be functionally deficient in selenium and vitamin E when grown in medium supplemented with fetal bovine serum. In the present experiments, the lack of significant bioavailable amounts of selenium in the medium was demonstrated by the finding of only low lev...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041230106
更新日期:1985-04-01 00:00:00
abstract::To evaluate the effects of fatty acids on endoplasmic reticulum (ER) stress, oxidative stress, and lipid damage. We treated BRL3A rat liver cells with, linoleic (LA), linolenic, oleic (OA), palmitic (PA), palmitoleic (POA), or stearic (SA) acid for 12 hr. The characteristics of cell lipid deposition, oxidative stress ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29553
更新日期:2020-09-01 00:00:00
abstract::Cover: Diagram illustrating the chromatic median analysis. See article by Pliss et al. on pages 427-439. ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24741
更新日期:2015-02-01 00:00:00
abstract::To evaluate the regulation of endothelial cell Cu,Zn-SOD, we have exposed bovine pulmonary artery endothelial cells in culture to hyperoxia and hypoxia, second messengers or related agonists, hormones, free radical generating systems, endotoxin, and cytokines and have measured Cu,Zn-SOD protein of these cells by an EL...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041530308
更新日期:1992-12-01 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is the most common endocrine abnormality in women of reproductive age. Although its clinical consequences have been known for a long time to extend beyond the reproductive system, with type-2 diabetes and obesity being the most common, the involvement of the ocular surface in PCOS has ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.25221
更新日期:2016-05-01 00:00:00
abstract::Dishevelled-associated activator of morphogenesis 1 (DAAM1) is a formin-family protein involved in nucleation of unbranched actin filaments and in cytoskeletal organization through Wnt-Dishevelled PCP pathway, which participates in essential biological processes, such as cell polarity, movement, and adhesion during mo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25330
更新日期:2016-10-01 00:00:00
abstract::Wnt/β-catenin signaling pathway plays a crucial role in diabetic cardiomyopathy (DCM), thus we aimed at investigating the effect of one therapeutic approach with resveratrol (RSV) given systemically and combined treatment of RSV with mesenchymal stem cells (MSCs) that was either RSV-preconditioned or not on Wnt/β-cate...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27947
更新日期:2019-07-01 00:00:00
abstract::Proteinuria in diabetic nephropathy has been correlated with reduction in heparan sulfate proteoglycan (HSPG) content of the glomerular basement membrane. We have previously shown that the underlying mechanism probably involves reduction in the synthesis by glomerular epithelial cells. In this study we explored whethe...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199604)167:1<131::AID-JCP1
更新日期:1996-04-01 00:00:00
abstract::The release of superoxide anion (O2-) by inflammatory macrophages, multinucleated giant cells, and epithelioid cells, obtained by the insertion of round glass coverslips into the subcutaneous tissue of mice, was investigated. O2- was shown to be spontaneously released by cells on the surface of glass coverslips implan...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041450313
更新日期:1990-12-01 00:00:00