Abstract:
:A separation procedure has been developed for mouse splenic T and B lymphocytes which is based on their differential agglutination by wheat germ agglutinin (WGA). In the presence of 50-100 micrograms/ml of WGA, multicellular aggregates are formed which are enriched in B cells. These aggregates can be separated from monodisperse T cells by gravity sedimentation and subsequently dissociated into single cells by treatment with N-acetylglucosamine (NAG). Immunocytochemical analyses and mitogenic assays indicate approximately 10-15% cross contamination of the resultant B and T cell fractions. The separation procedure is not only convenient and rapid but also allows the simultaneous recovery of viable T and B cells from the same spleen preparation.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Bourguignon LY,Rader RL,McMahon JTdoi
10.1002/jcp.1040990111subject
Has Abstractpub_date
1979-04-01 00:00:00pages
95-9issue
1eissn
0021-9541issn
1097-4652journal_volume
99pub_type
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