Rapid separation of mouse T and B lymphocytes using wheat germ agglutinin.

Abstract:

:A separation procedure has been developed for mouse splenic T and B lymphocytes which is based on their differential agglutination by wheat germ agglutinin (WGA). In the presence of 50-100 micrograms/ml of WGA, multicellular aggregates are formed which are enriched in B cells. These aggregates can be separated from monodisperse T cells by gravity sedimentation and subsequently dissociated into single cells by treatment with N-acetylglucosamine (NAG). Immunocytochemical analyses and mitogenic assays indicate approximately 10-15% cross contamination of the resultant B and T cell fractions. The separation procedure is not only convenient and rapid but also allows the simultaneous recovery of viable T and B cells from the same spleen preparation.

journal_name

J Cell Physiol

authors

Bourguignon LY,Rader RL,McMahon JT

doi

10.1002/jcp.1040990111

subject

Has Abstract

pub_date

1979-04-01 00:00:00

pages

95-9

issue

1

eissn

0021-9541

issn

1097-4652

journal_volume

99

pub_type

杂志文章
  • S100B causes apoptosis in a myoblast cell line in a RAGE-independent manner.

    abstract::S100B, a Ca(2+)-modulated protein with both intracellular and extracellular regulatory roles, is most abundant in astrocytes, is expressed in various amounts in several non-nervous cells and is also found in normal serum. Astrocytes secrete S100B, and extracellular S100B exerts trophic and toxic effects on neurons dep...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.10462

    authors: Sorci G,Riuzzi F,Agneletti AL,Marchetti C,Donato R

    更新日期:2004-05-01 00:00:00

  • Analysis of the protein glycosylation defect of a temperature-sensitive cell cycle mutant by the use of mutant cells overexpressing the human epidermal growth factor receptor after transfection of the gene.

    abstract::A temperature-sensitive mutant with a defect in glycoprotein synthesis and a cell cycle (G1)-specific arrest at the nonpermissive temperature (Tenner et al., J. Cell. Physiol., 90:145-160, 1977; Tenner and Scheffler, J. Cell. Physiol., 98:251-266, 1979) was investigated further after a human epidermal growth factor (E...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041330306

    authors: Feige JJ,Scheffler IE

    更新日期:1987-12-01 00:00:00

  • LOC103691336/miR-138-5p/BMPR2 axis modulates Mg-mediated osteogenic differentiation in rat femoral fracture model and rat primary bone marrow stromal cells.

    abstract::Intramedullary stabilization is frequently used to treat long bone fractures. Since implant removal can become technically very challenging with the potential to cause further tissue damage, biodegradable materials are emerging as alternative options. Magnesium (Mg)-based biodegradable implants have a controllable deg...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28736

    authors: Li D,Yu K,Xiao T,Dai Y,Liu L,Li H,Jiang D,Xiong L

    更新日期:2019-11-01 00:00:00

  • Proliferation of rat small hepatocytes requires follistatin expression.

    abstract::Small hepatocytes (SHs) are a subpopulation of hepatocytes that have high growth potential in culture and can differentiate into mature hepatocytes (MHs). The activin (Act)/follistatin (Fst) system critically contributes to homeostasis of cell growth in the normal liver. ActA and ActB consist of two disulfide-linked I...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22971

    authors: Ooe H,Chen Q,Kon J,Sasaki K,Miyoshi H,Ichinohe N,Tanimizu N,Mitaka T

    更新日期:2012-06-01 00:00:00

  • Breast cancer-derived exosomes: Tumor progression and therapeutic agents.

    abstract::Tumor cells secrete extracellular vesicles (EVs) for intercellular communication. EVs by transporting different proteins, nucleic acids, and lipids contribute to affect target cell function and fate. ‎EVs which originate directly from multivesicular bodies so-called exosomes have dramatically fascinated the attention ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.29668

    authors: Jabbari N,Akbariazar E,Feqhhi M,Rahbarghazi R,Rezaie J

    更新日期:2020-10-01 00:00:00

  • Modulation of drug resistance in homoharringtonine-resistant C-1300 neuroblastoma cells with cyclosporine A and dipyridamole.

    abstract::The development of resistance accounts for therapy failure in the majority of advanced cases of neuroblastoma in children. A new transplantable murine C-1300 neuroblastoma cell line was developed in vitro, by repeated exposure of a sensitive cell line to increasing, but sublethal, doses of Homoharringtonine (HHT). The...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041480319

    authors: Tebbi CK,Chervinsky D,Baker RM

    更新日期:1991-09-01 00:00:00

  • Variant HeLa cells selected for their resistance to ouabain.

    abstract::The cardiac glyoside, ouabain, normally kills HeLa cells at concentrations of about 10-7 M or greater. By treating a population of HeLa cells with increasingly higher concentrations of the drug, a vaiant population was obtained of HeLa cells capable of growing in medium containing 10-4 M ouabain. Inhibition of volume ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040850114

    authors: Rosenberg HM

    更新日期:1975-02-01 00:00:00

  • Immunomodulatory effects of nanocurcumin on Th17 cell responses in mild and severe COVID-19 patients.

    abstract::In novel coronavirus disease 2019 (COVID-19), the increased frequency and overactivation of T helper (Th) 17 cells and subsequent production of large amounts of proinflammatory cytokines result in hyperinflammation and disease progression. The current study aimed to investigate the therapeutic effects of nanocurcumin ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.30233

    authors: Tahmasebi S,El-Esawi MA,Mahmoud ZH,Timoshin A,Valizadeh H,Roshangar L,Varshoch M,Vaez A,Aslani S,Navashenaq JG,Aghebati-Maleki L,Ahmadi M

    更新日期:2020-12-28 00:00:00

  • Fluid shear-induced activation and cleavage of CD18 during pseudopod retraction by human neutrophils.

    abstract::Surface membrane expression and conformational activation of CD18 integrins into an open molecular configuration play critical roles in neutrophil ligand binding, membrane attachment, spreading on the endothelium, and cell migration to sites of inflammation. Previously, we observed pseudopod retraction and concomitant...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21235

    authors: Shin HY,Simon SI,Schmid-Schönbein GW

    更新日期:2008-02-01 00:00:00

  • Pur-alpha regulates RhoA developmental expression and downstream signaling.

    abstract::Pur-alpha is an essential protein for postnatal brain development which localizes specifically to dendrites where it plays a role in the translation of neuronal RNA. Mice lacking Pur-alpha display decreased neuronogenesis and impaired neuronal differentiation. Here we examined two Rho GTPases, Rac1 and RhoA, which pla...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24105

    authors: Mishra M,Del Valle L,Otte J,Darbinian N,Gordon J

    更新日期:2013-01-01 00:00:00

  • EGFL7: Master regulator of cancer pathogenesis, angiogenesis and an emerging mediator of bone homeostasis.

    abstract::Epidermal growth factor-like domain-containing protein 7 (EGFL7), a member of the epidermal growth factor (EGF)-like protein family, is a potent angiogenic factor expressed in many different cell types. EGFL7 plays a vital role in controlling vascular angiogenesis during embryogenesis, organogenesis, and maintaining s...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.26792

    authors: Hong G,Kuek V,Shi J,Zhou L,Han X,He W,Tickner J,Qiu H,Wei Q,Xu J

    更新日期:2018-11-01 00:00:00

  • A calcium requirement for movement of cultured cells.

    abstract::When calcium is removed from culture medium, motility of cultured cells is decreased. The effect is rapid, reversible and pronounced. Decreased motility is observed with normal mouse Balb/c 3T3 cells, mouse L929 cells, rat kidney fibroblasts and chick embryo fibroblasts. The calcium dependence of movement can be obser...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041010112

    authors: Moore L,Pastan I

    更新日期:1979-10-01 00:00:00

  • Interrelationships between water and cellular metabolism in Artemia cysts. IV. Adenosine 5'-triphosphate and cyst hydration.

    abstract::The concentration of ATP in cysts of the brine shrimp, Artemia salina, has been studied as a function of cyst hydration. Cysts dried over CaSO4 contain 0.02 gH2O/g cysts and 0.54 +/- 0.05 (S.D.) mumoles of ATP/g dried cysts. Addition of water up to 0.05 g/g cysts produced no net change in the level of ATP during incub...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040880205

    authors: Clegg JS,Cavagnaro J

    更新日期:1976-06-01 00:00:00

  • Differential proteomic profiling identifies novel molecular targets of pterostilbene against experimental diabetes.

    abstract::Pterostilbene (PTS), a naturally occurring stilbene, confers protection against oxidative and cytokine stress induced pancreatic β-cell apoptosis in vitro and in vivo. To provide insights into the molecular mechanism, we performed a proteomic study on the pancreas of PTS-treated diabetic mice using electrospray ioniza...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26835

    authors: Dornadula S,Thiruppathi S,Palanisamy R,Umapathy D,Suzuki T,K Mohanram R

    更新日期:2019-03-01 00:00:00

  • A potential role of knockout serum replacement as a porcine follicular fluid substitute for in vitro maturation: Lipid metabolism approach.

    abstract::The use of supplements, such as porcine follicular fluid (pFF), fetal bovine serum and human serum albumin are widely used during in vitro maturation (IVM) in different species but these supplements contain undefined components that cause technical difficulties in standardization and influence the efficiency of IVM. K...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26489

    authors: Jin JX,Lee S,Setyawan EMN,Taweechaipaisankul A,Kim GA,Han HJ,Ahn C,Lee BC

    更新日期:2018-09-01 00:00:00

  • Serum stimulation of human fibroblasts: effect on non-histones of nuclear ribonucleoprotein and chromatin.

    abstract::In quiescent human fibroblasts stimulated to proliferate by fresh medium plus 15% serum, no changes were seen in the incorporation of 3H tryptophan into the protein of nuclear ribonucleoprotein during the first three hours following re-feeding. This was in contrast to non-histone chromosomal proteins where the incorpo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040920115

    authors: Augenlicht LH,Lipkin M

    更新日期:1977-07-01 00:00:00

  • Curcumin, hemostasis, thrombosis, and coagulation.

    abstract::Atherothrombotic cardiovascular disease is a major cause of mortality throughout the world. Platelet activation and aggregation play a central role in hemostasis and thrombosis. Herbal medicines have been traditionally used in the management of cardiovascular disease and can help in modifying its progression, particul...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.26249

    authors: Keihanian F,Saeidinia A,Bagheri RK,Johnston TP,Sahebkar A

    更新日期:2018-06-01 00:00:00

  • Transforming growth factor-β signaling confers hepatic stellate cells progenitor features after partial hepatectomy.

    abstract::Liver regeneration involves not only hepatocyte replication but progenitor aggregation and scarring. Partial hepatectomy (PH), an established model for liver regeneration, reactivates transforming growth factor-β (TGF-β) signaling. Hepatic stellate cells (HSCs) are primarily responding cells for TGF-β and resident in ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29169

    authors: Chen Z,Wan L,Jin X,Wang W,Li D

    更新日期:2020-03-01 00:00:00

  • Adhesive substrates for fibronectin.

    abstract::In order to promote cell attachment, fibronectin must first undergo activation by a suitable substrate. In this study, 52 materials have been surveyed for their ability a) to bind fibronectin, b) to activate the cell-adhesive property of fibronectin, and c) to support the growth of cells. Many plastics, polysaccharide...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041090314

    authors: Klebe RJ,Bentley KL,Schoen RC

    更新日期:1981-12-01 00:00:00

  • IL-37-induced activation of glycogen synthase kinase 3β promotes IL-1R8/Sigirr phosphorylation, internalization, and degradation in lung epithelial cells.

    abstract::Interleukin (IL)-37 diminishes a variety of inflammatory responses through ligation to its receptor IL-1R8/Sigirr. Sigirr is a Toll like receptor/IL-1R family member. We have shown that Sigirr is not stable in response to IL-37 treatment. IL-37-induced Sigirr degradation is mediated by the ubiquitin-proteasome system,...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.30253

    authors: Li L,Wei J,Suber TL,Ye Q,Miao J,Li S,Taleb SJ,Tran KC,Tamaskar AS,Zhao J,Zhao Y

    更新日期:2021-01-05 00:00:00

  • Degradation of insulin-like growth factor-I receptor occurs via ubiquitin-proteasome pathway in human lung cancer cells.

    abstract::Insulin-like growth factor-I receptor (IGF-IR) is often overexpressed in malignant tumors, and is involved in the establishment and maintenance of malignant phenotypes. Tyrosine kinase receptor endocytosis is commonly triggered by ligand binding and occurs via clathrin-coated vescicles that transfer the receptor to th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20670

    authors: Carelli S,Di Giulio AM,Paratore S,Bosari S,Gorio A

    更新日期:2006-08-01 00:00:00

  • The origin of chicken hematopoietic colonies as assayed in semisolid agar.

    abstract::This investigation was undertaken to determine whether primitive stem cells and/or fully differentiated macrophages were the source of in vitro colonies derived from hematopoietic tissues. The chicken colony-forming cell (CFC) present in uncultured yolk sac was a nonadherent, presumably undifferentiated cell. The effi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040850104

    authors: Dodge WH,Silva RF,Moscovici C

    更新日期:1975-02-01 00:00:00

  • Timing of protooncogene expression varies in toxin-induced liver regeneration.

    abstract::Hepatic expression of the protooncogenes c-fos and c-myc occurs within 2 h after partial hepatectomy, and these immediate early genes are thought to prime the hepatocytes for subsequent proliferation. To examine whether such gene activation occurred in the setting of hepatocyte proliferation after toxic liver injury, ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041540212

    authors: Schmiedeberg P,Biempica L,Czaja MJ

    更新日期:1993-02-01 00:00:00

  • The alterations of circular RNA expression in plasma exosomes from patients with schizophrenia.

    abstract::Schizophrenia (SZ) is characterized by a high morbidity and disability rate and has gradually increased in rate and caused much burden. However, the pathogenesis of SZ is elusive and may include changes in the biological molecules in exosomes. In this study, we first compared the alterations of plasma exosomal circula...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29873

    authors: Tan G,Wang L,Liu Y,Zhang H,Feng W,Liu Z

    更新日期:2021-01-01 00:00:00

  • Precursor of mast cells fixed in the skin of mice.

    abstract::The presence and origin of mast-cell precursors fixed in the skin tissue of mice were investigated. Giant granules of beige (C57BL/6-bh'/bg', Chediak-Higashi syndrome) mice were used to distinguish different populations of mast cells. Pieces of the skin were grafted from the intact WBB6F1 (WB X C57BL/6)F1-+/+ mice int...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041080315

    authors: Matsuda H,Kitamura Y,Sonoda T,Imori T

    更新日期:1981-09-01 00:00:00

  • Downregulation and aberrant promoter methylation of p16INK4A: a possible novel heritable susceptibility marker to retinoblastoma.

    abstract::RB loss has long been recognized as the causative genetic alteration underlying retinoblastoma but it is increasingly evident that other alterations are required for the tumor to develop. Therefore, we set out to identify additional inheritable susceptibility markers and new potential preventive and therapeutic target...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22019

    authors: Indovina P,Acquaviva A,De Falco G,Rizzo V,Onnis A,Luzzi A,Giorgi F,Hadjistilianou T,Toti P,Tomei V,Pentimalli F,Carugi A,Giordano A

    更新日期:2010-04-01 00:00:00

  • Autocrine and paracrine growth stimulation of cells derived from human skin.

    abstract::A sensitive serum-free culture system was used to demonstrate that cells derived from normal human skin release soluble mediators which can modulate keratinocyte, melanocyte, and fibroblast growth in vitro. In M199 supplemented with epidermal growth factor, hydrocortisone, insulin, transferrin, triidothyronine, bovine...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041170215

    authors: Gilchrest BA,Karassik RL,Wilkins LM,Vrabel MA,Maciag T

    更新日期:1983-11-01 00:00:00

  • Primary human articular chondrocytes, dedifferentiated chondrocytes, and synoviocytes exhibit differential responsiveness to interleukin-4: correlation with the expression pattern of the common receptor gamma chain.

    abstract::Interleukin (IL)-4, which exhibits potent anti-inflammatory activities, is of potential therapeutic value in destructive arthropathies. To further define the response of human joint cells to IL-4, we analyzed the ability of this cytokine to modulate the effects of IL-1beta and growth factors. Freshly isolated chondroc...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.10121

    authors: Guicheux J,Palmer G,Relic B,Mezin F,Caverzasio J,Apostolides P,Gauchat JF,Gabay C,Guerne PA

    更新日期:2002-07-01 00:00:00

  • Involvement of the TREK-1 channel in human alveolar cell membrane potential and its regulation by inhibitors of the chloride current.

    abstract::K+ channels of the alveolar epithelium control the driving force acting on the ionic and solvent flow through the cell membrane contributing to the maintenance of cell volume and the constitution of epithelial lining fluid. In the present work, we analyze the effect of the Cl- channel inhibitors: (4-[(2-butyl-6,7-dich...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28396

    authors: Canella R,Martini M,Cavicchio C,Cervellati F,Benedusi M,Valacchi G

    更新日期:2019-08-01 00:00:00

  • Influence of colonic dialysis using Gubenxiezhuo on the distribution of gut microflora in uremia rats.

    abstract:OBJECTIVE:This study aimed to explore the underlying function of Gubenxiezhuo dialysis on the distribution of gut microflora uremia. METHODS:A uremia rat model was constructed, and the morphology of renal tissue was determined using the hematoxylin-eosin (H&E) staining. Moreover, the blood samples were collected and t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27845

    authors: He H,Hu P,Tang Y,Xu X

    更新日期:2019-07-01 00:00:00