Abstract：
:In order to promote cell attachment, fibronectin must first undergo activation by a suitable substrate. In this study, 52 materials have been surveyed for their ability a) to bind fibronectin, b) to activate the cell-adhesive property of fibronectin, and c) to support the growth of cells. Many plastics, polysaccharides, metals, and ceramics were found to support cell growth as well as the fibronectin-dependent attachment of cells. Several other substrates have been identified that were inactive in promoting either cell attachment or growth. Hydrophobic substrates were found to be active in fibronectin activation, whereas hydrophilic substrates were found to be inactive. Since fibronectin binds to substrata of extremely varied chemical composition, it is clear that the binding of fibronectin to such substrata is nonspecific in nature. Since protein pretreatment of all substrata, except collagen and poly(L-lysine), abolished the physical binding of fibronectin, the binding of fibronectin to artificial substrata is probably ascribable to a nonspecific hydrophobic protein-substratum interaction. In contrast, several lines of evidence indicate that the interaction between fibronectin and collagen displays biological specificity. Poly(hydroxyethylmethacrylate)(poly(HEMA)), which has previously been shown to be nonadhesive for cells, is demonstrated here to be unique in its inability to bind fibronectin. Addition of one part per million of an adhesive polymer to poly(HEMA) permits fibronectin binding to occur.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Klebe RJ,Bentley KL,Schoen RCdoi
10.1002/jcp.1041090314subject
Has Abstractpub_date
1981-12-01 00:00:00pages
481-8issue
3eissn
0021-9541issn
1097-4652journal_volume
109pub_type
杂志文章abstract：:Spinal cord injury (SCI) is a public health problem in the world. The SCI usually triggers an excessive inflammatory response that brings about a secondary tissue wreck leading to further cellular and organ dysfunction. Hence, there is great potential of reducing inflammation for therapeutic strategies of SCI. In this...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：2019-08-01 00:00:00
abstract：:Embryo implantation is essential for normal pregnancy, and the process of decidualization is critical for embryo implantation. However, the mechanism of decidualization during early pregnancy is still unknown. Forkhead box O3a (FOXO3a) is the most important functional transcription factor of the forkhead box family an...
journal_title：Journal of cellular physiology
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abstract：:Scatchard analysis of binding of 125I-basic fibroblast growth factor (FGF) to baby hamster kidney (BHK) cells revealed the presence of two binding sites: a high affinity site with KD of 20 pM and 80,000 sites per cell and a low affinity site with KD of about 2 nM and 600,000 sites per cell. The binding to the two site...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.1041310118
更新日期：1987-04-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28050
更新日期：2019-08-01 00:00:00
abstract：:Hemodynamic forces influence many endothelial cell functions. The coupling between hemodynamic forces and cell function could be mediated by mechano-sensitive ion channels present in the plasma membrane of endothelial cells. Because one of these channels is permeable to Ca2+, we tested whether hemodynamic forces influ...
journal_title：Journal of cellular physiology
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更新日期：1992-04-01 00:00:00
abstract：:Cultures of HeLa cells, strains S3G (HeLa65) and S3K (HeLa71) were grown in plastic dishes until firmly attached and were then treated with sonic dispersions of Rosenthal's phospholipase inhibitor. A rapid increase in alkaline phosphatase activity occurred following this treatment in the S3G strain (low inducible alka...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.1040920111
更新日期：1977-07-01 00:00:00
abstract：:We are interested in two aspects of a given type of metastatic breast cancer: which potentially cancer-relevant genes are expressed and which factors determine invasiveness. Using reverse transcription real-time PCR, we detected gene expression of 26 matrix metalloproteinases (MMPs) in MDA-MB-231 breast cancer cells, ...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.21417
更新日期：2008-08-01 00:00:00
abstract：:In culture, B16/C3 murine melanoma cells grown in the presence of serum undergo melanogenesis at a specific time after plating. At this time, melanin is synthesized intracellularly and then secreted into the extracellular culture fluid. We have found that melanin secretion is dependent on the presence of serum in the ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041130318
更新日期：1982-12-01 00:00:00
abstract：:Apoptosis constitutes a response of organisms to various physiological or pathological stimuli, and to different stresses. The ability of thermotolerance induced at a mild temperature of 40 degrees C to protect against activation of the apoptotic cascade by heat shock was investigated. When Chinese hamster ovary and h...
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doi：10.1002/jcp.20386
更新日期：2005-10-01 00:00:00
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doi：10.1002/jcp.20483
更新日期：2006-02-01 00:00:00
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journal_title：Journal of cellular physiology
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doi：10.1002/jcp.21353
更新日期：2008-06-01 00:00:00
abstract：:The aim of this study was to determine the effects and molecular mechanism of blue light emitting diode (LED) in tumor cells. A migration and invasion assay for the metastatic behavior of mouse colon cancer CT-26 and human fibrosarcoma HT-1080 cells was performed. Cancer cell migration-related proteins were identified...
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更新日期：2017-12-01 00:00:00
abstract：:Cell division in fertilized sea urchin eggs was reversibly inhibited when the ketoaldehyde phenyl glyoxal (PG) at a concentration of 0.1 mM was added to eggs for ten minutes prior to the formation of the mitotic spindle. We investigated whether inhibition of mitosis was due to PG binding to the cell surface (as previo...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041000119
更新日期：1979-07-01 00:00:00
abstract：:Migration and proliferation of smooth muscle cells (SMCs) are key events during neointimal formation in pathological conditions of vessels. Tenascin-C (TNC) is upregulated in the developing neointima of lesions. We evaluated the effects of TNC on responses of SMCs against platelet-derived growth factor (PDGF) stimulat...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22614
更新日期：2011-10-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
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更新日期：2018-01-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041430302
更新日期：1990-06-01 00:00:00
abstract：:The hypothesis of functional hemizygosity has been examined for the alpha-amanitin resistant (AmaR, a codominant marker) locus in a series of Chinese hamster cell lines. AmaR mutants were obtained from different cell lines, e.g., CHO, DHW, M3- 1 and CHO-Kl, at similar frequencies. After fractionation of different RNA ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040970321
更新日期：1978-12-01 00:00:00
abstract：:Binding of concanavalin A to isolated thymocyte membrane vesicles occurs through (a) numerous (approximately 6 x 106/cell equivalent) low-affinity sites (Ka = 1.3 x 105M-1) anf (b) fewer (approximately 0.4 x 106/cell equipment) specific receptors (Ka = 6.8 x 106 M-1) defined as 55,000 D glycoprotein and its multimers....
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doi：10.1002/jcp.1040890441
更新日期：1976-12-01 00:00:00
abstract：:Mutant forms of the p53 gene have been shown to cooperate with an activated ras gene in transforming primary cells in culture. The aberrant proteins encoded by p53 mutants are thought to act in a dominant negative manner in these assays. In vivo data, however, reveal that where p53 has undergone genetic change in tumo...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.1041480309
更新日期：1991-09-01 00:00:00
abstract：:As a natural metabolite of limonoids from Dictamnus dasycarpus, fraxinellone has been reported to be neuroprotective and anti-inflammatory. However, its influence on cellular metabolism remains largely unknown. In the present study, we investigated the effect of fraxinellone on cellular senescence-induced by oxidative...
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更新日期：2018-05-01 00:00:00
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journal_title：Journal of cellular physiology
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doi：10.1002/(SICI)1097-4652(199704)171:1<34::AID-JCP5>
更新日期：1997-04-01 00:00:00
abstract：:Reactive oxygen species (ROS) function as signaling molecules mainly by reversible oxidation of redox-sensitive target proteins. ROS can be produced in response to integrin ligation and growth factor stimulation through Rac1 and its effector protein NADPH oxidase. One of the central roles of Rac1-NADPH oxidase is acti...
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更新日期：2011-09-01 00:00:00
abstract：:Cadmium (Cd) is a toxic metal with multiple effects on cell signaling and cell death. We studied the effects of Cd(2+) on quiescent mouse mesangial cells in serum-free conditions. Cadmium induces cell death over 6 h through annexin V+ states without or with causing uptake of propidium iodide, termed apoptotic and apop...
journal_title：Journal of cellular physiology
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更新日期：2008-11-01 00:00:00
abstract：:While retinoids have been demonstrated to inhibit growth of many tumor cells, including SCC cells, the molecular mechanism by which retinoids suppress growth has not been elucidated. We previously found that the growth of SCC cells was significantly inhibited by all-trans-retinoic acid (all-trans-RA) treatment, and th...
journal_title：Journal of cellular physiology
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更新日期：2002-03-01 00:00:00
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doi：10.1002/(SICI)1097-4652(200001)182:1<88::AID-JCP10
更新日期：2000-01-01 00:00:00
abstract：:Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that is transiently expressed during the normal development of T and B lymphocytes. Phorbol 12-myristate 13-acetate (PMA) has been reported to induce maturation-like changes, including the loss of TdT, in many leukemic cell lines. We ...
journal_title：Journal of cellular physiology
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更新日期：1990-03-01 00:00:00
abstract：:Our previous studies have demonstrated that platelet-targeted factor IX (FIX) gene therapy can introduce sustained platelet-FIX expression in hemophilia B (FIXnull ) mice. In this study, we aimed to enhance platelet-FIX expression in FIXnull mice with O6 -methylguanine-DNA-methyltransferase (MGMT)-mediated in vivo dru...
journal_title：Journal of cellular physiology
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更新日期：2021-01-01 00:00:00
abstract：:Herbal-derived three-dimensional scaffolds have a unique structure that represents the natural cellular microenvironment and can be potentially used for tissue engineering applications. In the present study, cabbage (Cb) leaves were decellularized and then their characteristics, such as surface roughness, wettability,...
journal_title：Journal of cellular physiology
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更新日期：2020-12-30 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：2019-11-01 00:00:00
abstract：:Topical intranasal application of the antifungal Amphotericin B (AmphoB) has been shown as an effective medical treatment of chronic rhinosinusitis. Because this antibiotic forms channels in lipid membranes, we considered the possibility that it affects the properties and/or cell surface expression of ion channels/pum...
journal_title：Journal of cellular physiology
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