Abstract:
:Mutant forms of the p53 gene have been shown to cooperate with an activated ras gene in transforming primary cells in culture. The aberrant proteins encoded by p53 mutants are thought to act in a dominant negative manner in these assays. In vivo data, however, reveal that where p53 has undergone genetic change in tumors, both alleles have been affected. We previously identified a case of human acute myelogenous leukemia (AML) in which both alleles of the p53 gene had undergone independent missense mutations (at codons 135 cys to ser and 246 met to val). In these blasts, p53 mutations appear to be acting recessively. We have assayed the transforming potential of these p53 mutations, as well as that of another mutation at codon 273, also identified in a human neoplasm. Both mutations from the AML blasts (codon 135 and codon 246) confer transforming ability on the mutant protein. While transformation assays may define functionally different subsets of p53 mutations, the overexpression phenotype of mutants in this assay may not accurately reflect the pathological effects of p53 mutations in vivo.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Slingerland JM,Benchimol Sdoi
10.1002/jcp.1041480309subject
Has Abstractpub_date
1991-09-01 00:00:00pages
391-5issue
3eissn
0021-9541issn
1097-4652journal_volume
148pub_type
杂志文章abstract::Tissue expansion, a technique to enlarge the skin surface area with an expandable balloon, has been widely used in reconstructive surgery. Although the effect of tissue expansion on in vivo skin physiology and histology has been well documented, it remains unclear whether keratinocytes or other cell types are responsi...
journal_title:Journal of cellular physiology
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doi:10.1002/(SICI)1097-4652(199710)173:1<64::AID-JCP8>
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journal_title:Journal of cellular physiology
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更新日期:1999-06-01 00:00:00
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更新日期:1992-03-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:2020-02-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29872
更新日期:2021-01-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2019-06-01 00:00:00
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20285
更新日期:2005-07-01 00:00:00
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pub_type: 杂志文章,收录出版
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更新日期:2020-03-01 00:00:00
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journal_title:Journal of cellular physiology
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