Additional MDA-MB-231 breast cancer cell matrix metalloproteinases promote invasiveness.


:We are interested in two aspects of a given type of metastatic breast cancer: which potentially cancer-relevant genes are expressed and which factors determine invasiveness. Using reverse transcription real-time PCR, we detected gene expression of 26 matrix metalloproteinases (MMPs) in MDA-MB-231 breast cancer cells, including those of MMP-12, MMP-16 variant 2, MMP-19, MMP-20, MMP-21, MMP-23, MMP-24, MMP-25, MMP-25 variant 2, MMP-L1, MMP-26, MMP-27, and MMP-28, in contrast to the 13 MMPs detected until now in these cells. We found that MMP genes are expressed at widely different levels in these cells, over five orders of magnitude. After individual siRNA-induced depletions, we found that six additional species of cancer cell MMPs promote invasiveness in MDA-MB-231 cells: MMP-3, MMP-11, MMP-12, MMP-17, MMP-19, and MMP-23, thus raising the total to 12 endogenous MMPs which do so in these cells. The data support the conclusion that some cancer cell MMPs, although expressed at low levels, are needed for cancer trait in MDA-MB-231 cells, and that several endogenous MMPs play non-redundant roles in this process. The mRNA level of MMP-11, but not of other MMPs, rose substantially following individual siRNA-targeted depletion of cancer cell MMP-17 mRNA, while no MMP mRNA increased appreciably after degradation of other MMP mRNAs. This supports the conclusion that MMP-17 may be a member of an intracellular signaling pathway which downregulates MMP-11 mRNA.


J Cell Physiol


Hegedüs L,Cho H,Xie X,Eliceiri GL




Has Abstract


2008-08-01 00:00:00












  • High and low affinity binding sites for basic fibroblast growth factor on cultured cells: absence of a role for low affinity binding in the stimulation of plasminogen activator production by bovine capillary endothelial cells.

    abstract::Scatchard analysis of binding of 125I-basic fibroblast growth factor (FGF) to baby hamster kidney (BHK) cells revealed the presence of two binding sites: a high affinity site with KD of 20 pM and 80,000 sites per cell and a low affinity site with KD of about 2 nM and 600,000 sites per cell. The binding to the two site...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Moscatelli D

    更新日期:1987-04-01 00:00:00

  • Acute ethanol exposure-induced autophagy-mediated cardiac injury via activation of the ROS-JNK-Bcl-2 pathway.

    abstract::Binge drinking is associated with increased cardiac autophagy, and often triggers heart injury. Given the essential role of autophagy in various cardiac diseases, this study was designed to investigate the role of autophagy in ethanol-induced cardiac injury and the underlying mechanism. Our study showed that ethanol e...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhu Z,Huang Y,Lv L,Tao Y,Shao M,Zhao C,Xue M,Sun J,Niu C,Wang Y,Kim S,Cong W,Mao W,Jin L

    更新日期:2018-02-01 00:00:00

  • The metabolic and molecular mechanisms of hyperammonaemia- and hyperethanolaemia-induced protein catabolism in skeletal muscle cells.

    abstract::Hyperammonaemia and hyperethanolaemia are thought to be driving factors behind skeletal muscle myopathy in liver disease, that is, cirrhosis. Despite this, the singular and combined impacts of ethanol- and ammonia-induced protein catabolism are poorly defined. As such, we aimed to dissect out the effects of ammonia an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Crossland H,Smith K,Atherton PJ,Wilkinson DJ

    更新日期:2018-12-01 00:00:00

  • Erythroid colony formation in cultures of human marrow: effect of leukocyte conditioned medium.

    abstract::A method, based on the differing capacities of cells to adhere to a column of polyester fibres, has been described for separating human bone marrow cells into a nonadherent and an adherent fraction. The effect of this cell separation procedure on colony formation by erythroid progenitor cells was investigated. In cont...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Aye MT

    更新日期:1977-04-01 00:00:00

  • Hypoxia modulates cyclin and cytokine expression and inhibits peripheral mononuclear cell proliferation.

    abstract::Previously, we found that hypoxia can deeply affect the production of cytokines in human peripheral mononuclear cells (PBMC). Here, we demonstrated that the cycle progression of hypoxic PBMC, cultured in the presence or not of a specific T cell activator such as phytohaemagglutinin (PHA), was delayed when compared wit...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Naldini A,Carraro F

    更新日期:1999-12-01 00:00:00

  • Further evidence for an inhibitor of proliferation elaborated by normal human fibroblasts in culture: partial characterization of the inhibitor.

    abstract::We present evidence that extends our earlier preliminary report on the stimulatory effect of saline washes on confluent cells in culture (Lacey et al., '77). That work suggested that an inhibitory substance was being removed by the washes. The present work suggests that the inhibitor is in the 10,000-30,000 MW range, ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Strobel-Stevens JD,Lacey JC Jr

    更新日期:1981-02-01 00:00:00

  • Role of Rho in rabbit parietal cell.

    abstract::Rho is known as an important regulator of actin microfilament formation. We were led to study it because a dynamic rearrangement of actin filaments occurs during activation of gastric acid secretion. In order to use specific probes, the rabbit gastric gland culture system was employed and the various genes were expres...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tashiro K,Nagao T,Kurose H,Ichijo H,Urushidani T

    更新日期:2003-12-01 00:00:00

  • Mesenchymal stem cells contribute to endogenous FVIII:c production.

    abstract::Besides the liver, it has been difficult to identify which organ(s) and/or cellular component(s) contribute significantly to the production of human FVIII:c (FVIII). Thus far, only endothelial cells have been shown to constitute a robust extrahepatic source of FVIII, possibly explaining both the diverse presence of FV...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sanada C,Kuo CJ,Colletti EJ,Soland M,Mokhtari S,Knovich MA,Owen J,Zanjani ED,Porada CD,Almeida-Porada G

    更新日期:2013-05-01 00:00:00

  • Inhibitory effects of digoxin and ouabain on aldosterone synthesis in human adrenocortical NCI-H295 cells.

    abstract::The present study was to investigate the effects and action mechanisms of digoxin and ouabain on steroidogenesis in human adrenocortical NCI-H295 cells. Administration of digoxin or ouabain for 24 h decreased the basal and angiotensin II (Ang II)-stimulated release of aldosterone by NCI-H295 cells. The conversions of ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kau MM,Kan SF,Wang JR,Wang PS

    更新日期:2005-12-01 00:00:00

  • Insulin stimulus-secretion coupling is triggered by a novel thiazolidinedione/sulfonylurea hybrid in rat pancreatic islets.

    abstract::New compounds with promising antidiabetic activity were synthesized. For the first time, a portion of the glibenclamide molecule was bound to a part of the core structure of thiazolidinedione to evaluate insulin secretagogue activity. Following studies in our laboratory, 4-{2-[2-(3,4-dichlorophenyl)-4-oxo-1,3-thiazoli...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mendes CP,Postal BG,Oliveira GTC,Castro AJG,Frederico MJS,Moraes ALL,Neuenfeldt PD,Nunes RJ,Menegaz D,Silva FRMB

    更新日期:2018-01-01 00:00:00

  • Cinnamaldehyde regulates H2 O 2 -induced skeletal muscle atrophy by ameliorating the proteolytic and antioxidant defense systems.

    abstract::Skeletal muscle atrophy/wasting is associated with impaired protein metabolism in diverse physiological and pathophysiological conditions. Elevated levels of reactive oxygen species (ROS), disturbed redox status, and weakened antioxidant defense system are the major contributing factors toward atrophy. Regulation of p...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kaur N,Gupta P,Saini V,Sherawat S,Gupta S,Dua A,Kumar V,Injeti E,Mittal A

    更新日期:2019-05-01 00:00:00

  • Interleukin-8 enhances myocilin expression, Akt-FoxO3 signaling and myogenic differentiation in rat skeletal muscle cells.

    abstract::Interleukin (IL)-8 is released both in visceral adipose tissue and in contracting skeletal muscles. In this study, we examined cellular pathways associated with muscle hypertrophy, chosen on the basis of microRNA profiling, in differentiating rat primary skeletal muscle cells (RSkMC) treated with IL-8 (1 ng/ml) for 11...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Milewska M,Domoradzki T,Majewska A,Błaszczyk M,Gajewska M,Hulanicka M,Ciecierska A,Grzelkowska-Kowalczyk K

    更新日期:2019-11-01 00:00:00

  • Low- and high-affinity concanavalin a binding to thymocyte plasma membrane vesicles.

    abstract::Binding of concanavalin A to isolated thymocyte membrane vesicles occurs through (a) numerous (approximately 6 x 106/cell equivalent) low-affinity sites (Ka = 1.3 x 105M-1) anf (b) fewer (approximately 0.4 x 106/cell equipment) specific receptors (Ka = 6.8 x 106 M-1) defined as 55,000 D glycoprotein and its multimers....

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wallach DF,Schmidt-Ullrich R

    更新日期:1976-12-01 00:00:00

  • Nemo-like kinase regulates postnatal skeletal homeostasis.

    abstract::Nemo-like kinase (Nlk) is related to the mitogen-activated protein (MAP) kinases and known to regulate signaling pathways involved in osteoblastogenesis. In vitro Nlk suppresses osteoblastogenesis, but the consequences of the Nlk inactivation in the skeleton in vivo are unknown. To study the function of Nlk, Nlk(loxP/...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Canalis E,Kranz L,Zanotti S

    更新日期:2014-11-01 00:00:00

  • Involvement of glutathione in the inhibition of sea urchin egg mitosis by phenyl glyoxal.

    abstract::Cell division in fertilized sea urchin eggs was reversibly inhibited when the ketoaldehyde phenyl glyoxal (PG) at a concentration of 0.1 mM was added to eggs for ten minutes prior to the formation of the mitotic spindle. We investigated whether inhibition of mitosis was due to PG binding to the cell surface (as previo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Amy CM,Rebhun LI

    更新日期:1979-07-01 00:00:00

  • Production of transforming growth factor beta by human peripheral blood monocytes and neutrophils.

    abstract::Human peripheral blood monocytes and neutrophils secrete TGF-beta. Activation of monocytes with LPS stimulates the secretion of TGF-beta; however, the production of TGF-beta by neutrophils was not altered by treatment with LPS. The secreted TGF-beta appears to be in a fully active form since acid treatment of the cond...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Grotendorst GR,Smale G,Pencev D

    更新日期:1989-08-01 00:00:00

  • Transforming growth factor-β signaling confers hepatic stellate cells progenitor features after partial hepatectomy.

    abstract::Liver regeneration involves not only hepatocyte replication but progenitor aggregation and scarring. Partial hepatectomy (PH), an established model for liver regeneration, reactivates transforming growth factor-β (TGF-β) signaling. Hepatic stellate cells (HSCs) are primarily responding cells for TGF-β and resident in ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chen Z,Wan L,Jin X,Wang W,Li D

    更新日期:2020-03-01 00:00:00

  • Insulin-like growth factor-binding protein enhancement of insulin-like growth factor-I (IGF-I)-mediated DNA synthesis and IGF-I binding in a human breast carcinoma cell line.

    abstract::The insulin-like growth factors (IGFs) are potent mitogens for malignant cell proliferation. The majority of secreted IGFs are bound to specific IGF-binding proteins (IGFBPs) that are secreted by a large number of cells. These proteins may either inhibit or enhance IGF actions. Breast carcinoma cells secrete a variety...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chen JC,Shao ZM,Sheikh MS,Hussain A,LeRoith D,Roberts CT Jr,Fontana JA

    更新日期:1994-01-01 00:00:00

  • Retinoic acid induces adult muscle cell differentiation mediated by the retinoic acid receptor-alpha.

    abstract::Retinoic acid (RA) is known to induce differentiation in many cell systems. This induction is mediated by nuclear RA receptors (RARs), which act as transactivating factors belonging to the superfamily of steroid/thyroid nuclear receptors. In this study, effects of RA were studied in a mouse myogenic C2 cell line and i...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Halevy O,Lerman O

    更新日期:1993-03-01 00:00:00

  • ADP-Induced Ca2+ Signaling and Proliferation of Rat Ventricular Myofibroblasts Depend on Phospholipase C-Linked TRP Channels Activation Within Lipid Rafts.

    abstract::Nucleotides released during heart injury affect myocardium electrophysiology and remodeling through P2 purinoceptors activation in cardiac myofibroblasts. ATP and UTP endorse [Ca2+ ]i accumulation and growth of DDR-2/α-SMA-expressing myofibroblasts from adult rat ventricles via P2Y4 and P2Y2 receptors activation, resp...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Certal M,Vinhas A,Barros-Barbosa A,Ferreirinha F,Costa MA,Correia-de-Sá P

    更新日期:2017-06-01 00:00:00

  • Molecular and clinical characterization of Galectin-9 in glioma through 1,027 samples.

    abstract::In recent years, research on glioma immunotherapy have grown rapidly. However, the autoimmune-like side effects that are caused by blocking immunological checkpoints hinder their clinical application in gliomas currently. Galectin-9, a ligand for T-cell immunoglobulin mucin 3, has shed a new light on the treatment of ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yuan F,Ming H,Wang Y,Yang Y,Yi L,Li T,Ma H,Tong L,Zhang L,Liu P,Li J,Lin Y,Yu S,Ren B,Yang X

    更新日期:2020-05-01 00:00:00

  • A high throughput method to study the physiology of E2:ERα signaling in breast cancer cells.

    abstract::17β-estradiol (E2) regulates diverse physiological effects including cell proliferation through the estrogen receptor α (ERα), which as a transcription factor drives gene transcription and as an extra-nuclear localized receptor triggers the membrane-dependent activation of diverse kinase cascades. E2 also modifies ERα...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Leone S,Busonero C,Acconcia F

    更新日期:2018-05-01 00:00:00

  • Syndecans in chronic inflammatory and autoimmune diseases: Pathological insights and therapeutic opportunities.

    abstract::Syndecans (SDCs) are a family of heparan sulfate proteoglycans (HSPGs) glycoproteins ubiquitously expressed on the cell surfaces and extracellular matrix of all mammalian tissues. There are four mammalian syndecans, SDC-1 thorough 4, which play a critical role in cell adhesion, migration, proliferation, differentiatio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Agere SA,Kim EY,Akhtar N,Ahmed S

    更新日期:2018-09-01 00:00:00

  • Characterization of Cardiac Anoctamin1 Ca²⁺-Activated Chloride Channels and Functional Role in Ischemia-Induced Arrhythmias.

    abstract::Anoctamin1 (ANO1) encodes a Ca(2+)-activated chloride (Cl(-)) channel (CaCC) in variety tissues of many species. Whether ANO1 expresses and functions as a CaCC in cardiomyocytes remain unknown. The objective of this study is to characterize the molecular and functional expression of ANO1 in cardiac myocytes and the ro...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ye Z,Wu MM,Wang CY,Li YC,Yu CJ,Gong YF,Zhang J,Wang QS,Song BL,Yu K,Hartzell HC,Duan DD,Zhao D,Zhang ZR

    更新日期:2015-02-01 00:00:00

  • Growth stimulating activity in bovine pituitary extract specific for a rat mammary carcinoma cell line.

    abstract::We report the isolation of a bovine pituitary growth factor (MGF) for a rat mammary carcinoma cell line, 64-24, which was isolated from a highly hormone-dependent mammary tumor. The MGF has been partially purified by a series of Diaflo ultrafiltration membrane sievings, isoelectric focusing and Sephadex columns. The M...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kano-Sueoka T,Campbell GR,Gerber M

    更新日期:1977-12-01 00:00:00

  • Persistent effects of phorbol 12-myristate 13-acetate: possible implication of vesicle traffic.

    abstract::Relative to their normal counterparts, transformed epithelial cells have a distinctive and quantifiable three-dimensional shape. Biophysical and mathematical methods are used to distinguish these extremes in cells from two lines, cultured from rat liver and tracheal epithelium, respectively. Cells adopted a more trans...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Heckman CA,Plummer HK 3rd,Runyeon CS

    更新日期:1996-01-01 00:00:00

  • ZC3H13 suppresses colorectal cancer proliferation and invasion via inactivating Ras-ERK signaling.

    abstract::ZC3H13 is a canonical CCCH zinc finger protein, which harbors a somatic frame-shift mutation in colorectal cancer (CRC). However, its expression and biological function were still uncertain. In the current study, we found that ZC3H13 was served as a tumor suppressor in CRC cells, which decreased the expression of Snai...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhu D,Zhou J,Zhao J,Jiang G,Zhang X,Zhang Y,Dong M

    更新日期:2019-06-01 00:00:00

  • The increase in hormone-stimulated adenylate cyclase activity following Rous sarcoma virus transformation.

    abstract::Rous sarcoma virus (RSV)-infected chicken embryo cells were used to study the effect of viral transformation on the hormone-stimulated synthesis of cyclic AMP. Transformation by RSV greatly increased the cells' ability to synthesize and accumulate cyclic AMP in response to the beta-adrenergic agonist isoproterenol as ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Howard RF,Friis RR,Sheppard JR,Glossmann H

    更新日期:1982-06-01 00:00:00

  • Synthesis and distribution of cytoskeletal elements in endothelial cells as a function of cell growth and organization.

    abstract::Confluent cultures of adult bovine aortic endothelial (ABAE), corneal endothelial (BCE), and fetal bovine heart endothelial (FBHE) cells form a monolayer of highly flattened, closely apposed, and nonoverlapping cells. In ABAE and BCE cultures, this is associated with a 50-fold decrease in the rate of DNA sythesis and ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Savion N,Vlodavsky I,Greenburg G,Gospodarowicz D

    更新日期:1982-02-01 00:00:00

  • Calcium uptake by isolated rat intestinal cells.

    abstract::Intestinal cells were isolated by a combination of mechanical and enzymatic means, and their calcium uptake was assayed by a rapid filtration procedure. Calcium uptake was a time- and concentration-dependent process that was markedly elevated at 25 and 37 degrees C, as compared to 0 degree C. Cells isolated from rat d...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bronner F,Pansu D,Bosshard A,Lipton JH

    更新日期:1983-09-01 00:00:00