Phenotypic characterization of ouabain-resistant Aedes albopictus cells.

Abstract:

:The phenotype of a ouabain-resistant Aedes albopictus cell line has been partially characterized. Treatment of ouabain-sensitive cells with 0.005-1.0 mM ouabain resulted in an 80% reduction in the uptake of 86rubidium (86Rb+), an ion with an affinity for the K+ pump binding site; ouabain-resistant cells showed only a 40% reduction with 1.0 mM ouabain. When ouabain-sensitive cells were incubated in the presence of ouabain (0.1 mM) for one and one-half to three hours, the molar ratio of intracellular Na+/K+ rose from 0.2 to 4.2. In ouabain-resistant cells, a similar treatment had very little effect. Based on [3H] ouabain-binding studies, ouabain-resistant cells were estimated to have 60% fewer binding sites per cell than ouabain-sensitive cells. The spontaneous mutation rate from ouabain sensitivity to ouabain resistance was calculated to be 1-6 x 10(-8) mutations/cell/generation, a value similar to that reported for mammalian cells at the analogous locus.

journal_name

J Cell Physiol

authors

Mento SJ,Malinoski F,Stollar V

doi

10.1002/jcp.1041010317

subject

Has Abstract

pub_date

1979-12-01 00:00:00

pages

515-21

issue

3

eissn

0021-9541

issn

1097-4652

journal_volume

101

pub_type

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