Tumor necrosis factor alpha: posttranscriptional stabilization of WAF1 mRNA in p53-deficient human leukemic cells.

Abstract:

:The p53 protein directly regulates the expression of the WAF1 (wild-type p53-activated fragment 1) protein which is a cyclin-dependent kinase inhibitor (CDK1). DNA damaging agents such as ionizing or UV radiation, and some chemical agents induce WAF1 in wild-type p53 containing cells, thereby halting cell cycle progression. WAF1 expression is also induced through a p53-independent pathway. Tumor necrosis factor alpha (TNF alpha) is a cytotoxic/cytostatic compound for some human cancer cells. We examined a series of myeloid leukemic cell lines that expressed either no p53 (HL-60, K562) or mutant inactive p53 (KG-1, KCL22,THP-1, U937). The KG-1, HL-60, K562, and KCL22 myeloid leukemic cells increased their levels of WAF1 mRNA in the presence of TNF alpha. We focused on KG-1 cells to determine how TNF alpha modulated WAF1 expression. WAF1 mRNA increased in a dose-dependent manner in the cells after exposure to increasing concentrations of TNF alpha, and this increase occurred in the absence of new protein synthesis. An increase of WAF1 protein and a concominant decrease of cyclin-dependent kinase 2 activity also was found in KG-1 cells. Flow cytometry using 5-bromo-2'-deoxyuridine showed an increase in the proportion of TNF alpha- treated KG-1 cells in the G0/G1 phase of the cell cycle. TNF alpha enhanced the rate of WAF1 transcription only 1.4 fold in TNF alpha-treated KG-1 cells as compared to untreated cells. Notably, however, the half-life (t 1/2) of WAF1 mRNA in TNF alpha-treated cells was 2.5 hours as compared to 0.5 hours in untreated cells. These results indicate that TNF alpha increases WAF1 levels at least in part via a postttranscriptional stabilization of the mRNA; and TNF alpha may mediate its cytostatic effects through WAF1 in some cell types.

journal_name

J Cell Physiol

authors

Shiohara M,Akashi M,Gombart AF,Yang R,Koeffler HP

doi

10.1002/(SICI)1097-4652(199603)166:3<568::AID-JCP1

subject

Has Abstract

pub_date

1996-03-01 00:00:00

pages

568-76

issue

3

eissn

0021-9541

issn

1097-4652

pii

10.1002/(SICI)1097-4652(199603)166:3<568::AID-JCP1

journal_volume

166

pub_type

杂志文章
  • Fibroblast-dependent growth of mouse mast cells in vitro: duplication of mast cell depletion in mutant mice of W/Wv genotype.

    abstract::In spite of the apparent depletion of mast cells in tissues of mutant mice of W/Wv genotype, cells with many features of mast cells do develop when bone marrow cells of W/Wv mice are cultured in the presence of pokeweed mitogen-stimulated spleen cell-conditioned medium (PWM-SCM). In order to resolve this discrepancy a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041340109

    authors: Fujita J,Nakayama H,Onoue H,Kanakura Y,Nakano T,Asai H,Takeda S,Honjo T,Kitamura Y

    更新日期:1988-01-01 00:00:00

  • Thermotolerance induced at a mild temperature of 40 degrees C protects cells against heat shock-induced apoptosis.

    abstract::Apoptosis constitutes a response of organisms to various physiological or pathological stimuli, and to different stresses. The ability of thermotolerance induced at a mild temperature of 40 degrees C to protect against activation of the apoptotic cascade by heat shock was investigated. When Chinese hamster ovary and h...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20386

    authors: Bettaieb A,Averill-Bates DA

    更新日期:2005-10-01 00:00:00

  • Functional alpha1- and beta2-adrenergic receptors in human osteoblasts.

    abstract::Central (hypothalamic) control of bone mass is proposed to be mediated through beta2-adrenergic receptors (beta2-ARs). While investigations in mouse bone cells suggest that epinephrine enhances both RANKL and OPG mRNA via both beta-ARs and alpha-ARs, whether alpha-ARs are expressed in human bone cells is controversial...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21761

    authors: Huang HH,Brennan TC,Muir MM,Mason RS

    更新日期:2009-07-01 00:00:00

  • Downregulated microRNA-135a ameliorates rheumatoid arthritis by inactivation of the phosphatidylinositol 3-kinase/AKT signaling pathway via phosphatidylinositol 3-kinase regulatory subunit 2.

    abstract::Synovial fibroblasts (SFs) of rheumatoid arthritis (RA) are phenotypically aggressive, typically progressing into arthritic cartilage degradation. Throughout our study, we made explorations into the effects of microRNA-135a (miR-135a) on the SFs involved in RA by mediating the phosphatidylinositol 3-kinase (PI3K)/AKT ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28390

    authors: Qu Y,Zhang YP,Wu J,Jie LG,Deng JX,Zhao DB,Yu QH

    更新日期:2019-08-01 00:00:00

  • The role of miR-34 in cancer drug resistance.

    abstract::Resistance to conventional chemotherapy remains a major cause of cancer relapse and cancer-related deaths. Therefore, there is an urgent need to overcome resistance barriers. To improve cancer treatment approaches, it is critical to elucidate the basic mechanisms underlying drug resistance. Increasingly, the mechanism...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.29640

    authors: Naghizadeh S,Mohammadi A,Duijf PHG,Baradaran B,Safarzadeh E,Cho WC,Mansoori B

    更新日期:2020-10-01 00:00:00

  • Sulfated malto-oligosaccharides bind to basic FGF, inhibit endothelial cell proliferation, and disrupt endothelial cell tube formation.

    abstract::The interaction of basic FGF (bFGF) with heparin, heparan sulfate and related sugars can potentiate or antagonize bFGF activity, depending on the size of the saccharide used. Oligosaccharides based on heparin structures, as small as six sugar residues, have been demonstrated to bind to bFGF and block its activity, whi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199609)168:3<657::AID-JCP1

    authors: Foxall C,Wei Z,Schaefer ME,Casabonne M,Fugedi P,Peto C,Castellot JJ Jr,Brandley BK

    更新日期:1996-09-01 00:00:00

  • Growth stimulating activity in bovine pituitary extract specific for a rat mammary carcinoma cell line.

    abstract::We report the isolation of a bovine pituitary growth factor (MGF) for a rat mammary carcinoma cell line, 64-24, which was isolated from a highly hormone-dependent mammary tumor. The MGF has been partially purified by a series of Diaflo ultrafiltration membrane sievings, isoelectric focusing and Sephadex columns. The M...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040930313

    authors: Kano-Sueoka T,Campbell GR,Gerber M

    更新日期:1977-12-01 00:00:00

  • Sensitivity of excision repair in normal human, xeroderma pigmentosum variant and Cockayne's syndrome fibroblasts to inhibition by cytosine arabinoside.

    abstract::Inhibition of the gap-filling, polymerizing step of excision repair by 1-beta-D-arabinofuranosylcytosine (ara-C) after irradiation with ultraviolet light in human diploid fibroblasts resulted in the formation of persistent DNA strand breaks in G1, G2, and plateau phase cells, but not in S phase cells. Addition of hydr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041080207

    authors: Cleaver JE

    更新日期:1981-08-01 00:00:00

  • Mechanisms controlling neurite outgrowth in a pheochromocytoma cell line: the role of TRPC channels.

    abstract::Transient Receptor Potential Canonical (TRPC) channels are implicated in modulating neurite outgrowth. The expression pattern of TRPCs changes significantly during brain development, suggesting that fine-tuning TRPC expression may be important for orchestrating neuritogenesis. To study how alterations in the TRPC expr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22855

    authors: Kumar S,Chakraborty S,Barbosa C,Brustovetsky T,Brustovetsky N,Obukhov AG

    更新日期:2012-04-01 00:00:00

  • Keratins and epidermolysis bullosa simplex.

    abstract::Keratin intermediate filaments play an important role in maintaining the integrity of the skin structure. Understanding the importance of this subject is possible with the investigation of keratin defects in epidermolysis bullosa simplex (EBS). Nowadays, in addition to clinical criteria, new molecular diagnostic metho...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.26898

    authors: Khani P,Ghazi F,Zekri A,Nasri F,Behrangi E,Aghdam AM,Mirzaei H

    更新日期:2018-01-01 00:00:00

  • Complexity on modulation of NF-κB pathways by hepatitis B and C: A double-edged sword in hepatocarcinogenesis.

    abstract::Nuclear factor-κB (NF-κB), a family of master regulated dimeric transcription factors, signaling transduction pathways are active players in the cell signaling that control vital cellular processes, including cell growth, proliferation, differentiation, apoptosis, morphogenesis, angiogenesis, and immune responses. Nev...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.28249

    authors: Shokri S,Mahmoudvand S,Taherkhani R,Farshadpour F,Jalalian FA

    更新日期:2019-02-11 00:00:00

  • Fibronectin Fiber Extension Decreases Cell Spreading and Migration.

    abstract::The extracellular matrix (ECM) is present in a range of molecular conformations and intermolecular arrangements. Fibronectin (Fn) molecules that constitute fibers within the ECM can exist in a variety of conformations that result from both mechanical stress and chemical factors such as allosteric binding partners. The...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25271

    authors: Hubbard B,Buczek-Thomas JA,Nugent MA,Smith ML

    更新日期:2016-08-01 00:00:00

  • Altering microtubule stability affects microtubule clearance and nuclear extrusion during erythropoiesis.

    abstract::Mammalian erythrocytes are highly specialized cells that have adapted to lose their nuclei and cellular components during maturation to ensure oxygen delivery. Nuclear extrusion, the most critical event during erythropoiesis, represents an extreme case of asymmetric partitioning that requires a dramatic reorganization...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28582

    authors: Xie S,Yan B,Feng J,Wu Y,He N,Sun L,Zhou J,Li D,Liu M

    更新日期:2019-11-01 00:00:00

  • Role of the sarcoplasmic reticulum in regulating the activity-dependent expression of the glycogen phosphorylase gene in contractile skeletal muscle cells.

    abstract::Nerve-evoked contractile activity in skeletal muscle regulates transcript and protein levels of many metabolic genes in a coordinate fashion, including the muscle isozyme of glycogen phosphorylase (MGP). Cellular signaling mechanisms mediating the activity-dependent modulation of MGP transcript levels were investigate...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/1097-4652(200011)185:2<184::AID-JCP3>3.0.C

    authors: Vali S,Carlsen R,Pessah I,Gorin F

    更新日期:2000-11-01 00:00:00

  • Induction of HIF-1alpha expression by intermittent hypoxia: involvement of NADPH oxidase, Ca2+ signaling, prolyl hydroxylases, and mTOR.

    abstract::Sleep-disordered breathing with recurrent apnea (periodic cessation of breathing) results in chronic intermittent hypoxia (IH), which leads to cardiovascular and respiratory pathology. Molecular mechanisms underlying IH-evoked cardio-respiratory co-morbidities have not been delineated. Mice with heterozygous deficienc...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21537

    authors: Yuan G,Nanduri J,Khan S,Semenza GL,Prabhakar NR

    更新日期:2008-12-01 00:00:00

  • Heparan sulfates of mouse cells. Analysis of parent and transformed 3T3 cell lines.

    abstract::Heparan sulfate from the surface of a variety of mouse cells at different cell densities was examined by ion-exchange chromatography. The results of this analysis show that: (1) The heparan sulfate from new isolates of Swiss 3T3 cells transformed by SV40 virus (a DNA tumor virus) elutes from DEAE-cellulose at a lower ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040900108

    authors: Underhill CB,Keller JM

    更新日期:1977-01-01 00:00:00

  • Spindle pole fragmentation due to proteasome inhibition.

    abstract::During interphase, the centrosome concentrates cell stress response molecules, including chaperones and proteasomes, into a proteolytic center. However, whether the centrosome functions as proteolytic center during mitosis is not known. In this study, cultured mammalian cells were treated with the proteasome inhibitor...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20335

    authors: Ehrhardt AG,Sluder G

    更新日期:2005-09-01 00:00:00

  • Iron specific growth inhibition of Burkitt's lymphoma cells in vitro, associated with a decrease in translocated c-myc expression.

    abstract::The cellular proto-oncogene c-myc is an important transcription factor that plays a role in several cellular activities such as proliferation, differentiation, and apoptosis. It follows that regulation of c-myc expression is crucial for maintaining cell integrity. Amplification or translocation can convert this proto-...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20229

    authors: Habel ME,Lemieux R,Jung D

    更新日期:2005-04-01 00:00:00

  • Regulation of protein phosphatase 2A-mediated recruitment of IQGAP1 to beta1 integrin by EGF through activation of Ca2+/calmodulin-dependent protein kinase II.

    abstract::Maintenance of beta1 integrin-mediated cell adhesion in quiescent human mammary epithelial (HME) cells requires protein phosphatase (PP) 2A for not only dephosphorylation of beta1 integrin but also recruitment of IQGAP1 to Rac-bound beta1 integrin. However, how PP2A-dependent regulatory machinery of cell adhesion resp...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20657

    authors: Takahashi K,Suzuki K

    更新日期:2006-07-01 00:00:00

  • Inhibition of PKC-Induced COX-2 and IL-8 Expression in Human Breast Cancer Cells by Glucosamine.

    abstract::Breast cancer is a common cancer leading to many deaths among females. Cyclooxygenase-2 (COX-2) and interleukin-8 (IL-8) are two highly expressed inflammatory mediators to be induced by the protein kinase C (PKC) signaling via various inflammatory stimuli and both contribute significantly to cancer metastasis/progress...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24955

    authors: Chou WY,Chuang KH,Sun D,Lee YH,Kao PH,Lin YY,Wang HW,Wu YL

    更新日期:2015-09-01 00:00:00

  • Amphotericin B resistance is recessive in Chinese hamster hybrid cells.

    abstract::Previous studies from our laboratory have shown that Chinese hamster V79 cells mutated to high level resistance to amphotericin B have a lower cellular level of cholesterol, the target molecule for the polyene antibiotic. Two amphotericin B-resistant (AMBR) mutants were each hybridized to their parental amphotericin B...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041060106

    authors: Hidaka K,Akiyama SI,Kuwano M

    更新日期:1981-01-01 00:00:00

  • Decoupled syndecan 1 mRNA and protein expression is differentially regulated by angiotensin II in macrophages.

    abstract::It has been established that syndecan-1 is an important modulator of events relevant to acute tissue repair and chronic injury responses. The current studies were designed to examine syndecan-1 expression during atherosclerotic lesion formation and whether angiotensin II influences syndecan-1 expression in macrophages...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21271

    authors: Wang W,Haller CA,Wen J,Wang P,Chaikof EL

    更新日期:2008-03-01 00:00:00

  • Infiltration anesthetic lidocaine inhibits cancer cell invasion by modulating ectodomain shedding of heparin-binding epidermal growth factor-like growth factor (HB-EGF).

    abstract::Although the mechanism is unknown, infiltration anesthetics are believed to have membrane-stabilizing action. We report here that such a most commonly used anesthetic, lidocaine, effectively inhibited the invasive ability of human cancer (HT1080, HOS, and RPMI-7951) cells at concentrations used in surgical operations ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.10145

    authors: Mammoto T,Higashiyama S,Mukai M,Mammoto A,Ayaki M,Mashimo T,Hayashi Y,Kishi Y,Nakamura H,Akedo H

    更新日期:2002-09-01 00:00:00

  • Chromosome balance and the control of malignancy.

    abstract::The Giemsa banding pattern of the chromosomes has been analyzed in a line of transformed golden hamster cells, revertant and re-revertant cells and their tumors. The transformed and re-revertant cells were malignant in vivo and had gained an additional chromosome 5(7). Revertants with a suppression of malignancy lost ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040870112

    authors: Bloch-Shtacher N,Sachs L

    更新日期:1976-01-01 00:00:00

  • Lipocalin-2 (NGAL) Attenuates Autophagy to Exacerbate Cardiac Apoptosis Induced by Myocardial Ischemia.

    abstract::Lipocalin-2 (Lcn2; also termed neutrophil gelatinase-associated lipocalin (NGAL)) levels correlate positively with heart failure (HF) yet mechanisms via which Lcn2 contributes to the pathogenesis of HF remain unclear. In this study, we used coronary artery ligation surgery to induce ischemia in wild-type (wt) mice and...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25672

    authors: Sung HK,Chan YK,Han M,Jahng JWS,Song E,Danielson E,Berger T,Mak TW,Sweeney G

    更新日期:2017-08-01 00:00:00

  • LPS Induces Hyper-Permeability of Intestinal Epithelial Cells.

    abstract::Necrotizing Enterocolitis (NEC) is a severe inflammatory disorder leading to high morbidity and mortality rates. A growing body of evidence demonstrate the key role of the Toll like receptor 4 (TLR4) in NEC. This membranal receptor recognizes lipopolysaccharides (LPS) from the bacterial wall and triggers an inflammato...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25435

    authors: Bein A,Zilbershtein A,Golosovsky M,Davidov D,Schwartz B

    更新日期:2017-02-01 00:00:00

  • Characterization of Cardiac Anoctamin1 Ca²⁺-Activated Chloride Channels and Functional Role in Ischemia-Induced Arrhythmias.

    abstract::Anoctamin1 (ANO1) encodes a Ca(2+)-activated chloride (Cl(-)) channel (CaCC) in variety tissues of many species. Whether ANO1 expresses and functions as a CaCC in cardiomyocytes remain unknown. The objective of this study is to characterize the molecular and functional expression of ANO1 in cardiac myocytes and the ro...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24709

    authors: Ye Z,Wu MM,Wang CY,Li YC,Yu CJ,Gong YF,Zhang J,Wang QS,Song BL,Yu K,Hartzell HC,Duan DD,Zhao D,Zhang ZR

    更新日期:2015-02-01 00:00:00

  • Role of epimorphin in bile duct formation of rat liver epithelial stem-like cells: involvement of small G protein RhoA and C/EBPβ.

    abstract::Epimorphin/syntaxin 2 is a high conserved and very abundant protein involved in epithelial morphogenesis in various organs. We have shown recently that epimorphin (EPM), a protein exclusively expressed on the surface of hepatic stellate cells and myofibroblasts of the liver, induces bile duct formation of hepatic stem...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22625

    authors: Jia Y,Yao H,Zhou J,Chen L,Zeng Q,Yuan H,Shi L,Nan X,Wang Y,Yue W,Pei X

    更新日期:2011-11-01 00:00:00

  • miR-142a-5p promoted osteoblast differentiation via targeting nuclear factor IA.

    abstract::miR-142a-5p plays critical roles in multiple biological processes and diseases, such as inflammation and tumorigenesis. However, it remains to be explored if and how miR-142a-5p contributes to osteoblast differentiation. In this study, our results showed that miR-142a-5p was highly expressed in bone tissue of mice and...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29963

    authors: Yuan H,Li M,Feng X,Zhu E,Wang B

    更新日期:2021-03-01 00:00:00

  • Emerging insights into molecular mechanisms underlying pyroptosis and functions of inflammasomes in diseases.

    abstract::Pyroptosis is a form of necrotic and inflammatory programmed cell death, which could be characterized by cell swelling, pore formation on plasma membranes, and release of proinflammatory cytokines (IL-1β and IL-18). The process of pyroptosis presents as dual effects: protecting multicellular organisms from microbial i...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.29268

    authors: Lu F,Lan Z,Xin Z,He C,Guo Z,Xia X,Hu T

    更新日期:2020-04-01 00:00:00