Profiling of retinoid mediated gene expression in synchronized human SCC cells using Atlas human cDNA expression arrays.

Abstract:

:While retinoids have been demonstrated to inhibit growth of many tumor cells, including SCC cells, the molecular mechanism by which retinoids suppress growth has not been elucidated. We previously found that the growth of SCC cells was significantly inhibited by all-trans-retinoic acid (all-trans-RA) treatment, and this inhibition was dependent on the binding and activation of RARs. These nuclear receptors bind retinoids and alter the rate of transcription of specific genes. To identify targets of the activated RARs which mediate growth inhibition, we growth arrested SCC-25 cells in G-0 and examined the effect of all-trans-RA on synchronized SCC-25 cells. All-trans-RA inhibited G-1 progression in quiescent SCC-25 cells stimulated by FBS. More specifically, we found that the all-trans-RA execution point maps to mid/late G-1, 6 to 10 h after stimulation. Using this synchronized cell system, we examined the expression of cell cycle regulatory genes in quiescent SCC-25 cells stimulated with FBS and treated with all-trans-RA. We found few changes in expression of these genes which could account for all-trans-RA inhibition of SCC-25 cell growth. In order to compare the patterns of expression of a wider selection of genes in all-trans-RA treated and non-treated SCC-25 cells, we have used expression array technology. We successfully performed expression profiling experiments on the Atlas Human cDNA arrays which contain 1176 human genes. We have identified several up-regulated and several down-regulated gene expression changes mediated by all-trans-RA treatment in synchronized SCC-25 cells. This novel information will be useful in defining the mechanism by which retinoids suppress the growth of SCC cells.

journal_name

J Cell Physiol

authors

Le Q,Soprano DR,Soprano KJ

doi

10.1002/jcp.10051

keywords:

subject

Has Abstract

pub_date

2002-03-01 00:00:00

pages

345-55

issue

3

eissn

0021-9541

issn

1097-4652

pii

10.1002/jcp.10051

journal_volume

190

pub_type

杂志文章
  • Synthesis of basic fibroblast growth factor upon differentiation of rat mammary epithelial to myoepithelial-like cells in culture.

    abstract::Acidic fibroblast growth factor (aFGF) mRNA was detected in a rat mammary fibroblastic cell line, but not in rat mammary epithelial cell lines or myoepithelial-like cell lines. Basic FGF (bFGF) mRNA was detected in both the fibroblasts and the myoepithelial-like cells, but was absent from the epithelial cells. A serie...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041440220

    authors: Barraclough R,Fernig DG,Rudland PS,Smith JA

    更新日期:1990-08-01 00:00:00

  • ELP2 negatively regulates osteoblastic differentiation impaired by tumor necrosis factor α in MC3T3-E1 cells through STAT3 activation.

    abstract::Tumor necrosis factor-α (TNF-α) is a pluripotent signaling molecule. The biological effect of TNF-α includes slowing down osteogenic differentiation, which can lead to bone dysplasia in long-term inflammatory microenvironments. Signal transducer and activator of transcription 3 (STAT3)-interacting protein 1 (StIP1, al...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28440

    authors: Xu CP,Sun HT,Yang YJ,Cui Z,Wang J,Yu B,Wang FZ,Yang QP,Qi Y

    更新日期:2019-08-01 00:00:00

  • Angiotensin type 1 receptor autoantibody from preeclamptic patients induces human fetoplacental vasoconstriction.

    abstract::Increased vascular resistance in the fetoplacental circulation is a characteristic of preeclampsia. However, the potential molecular mechanisms of this condition remain obscure. The current study aimed to determine the direct effect of the peptide antigen corresponding to the second extracellular loop of the angiotens...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24113

    authors: Zhang S,Zheng R,Yang L,Zhang X,Zuo L,Yang X,Bai K,Song L,Tian J,Yang J,Liu H

    更新日期:2013-01-01 00:00:00

  • Effect of strain on human keratinocytes in vitro.

    abstract::Tissue expansion, a technique to enlarge the skin surface area with an expandable balloon, has been widely used in reconstructive surgery. Although the effect of tissue expansion on in vivo skin physiology and histology has been well documented, it remains unclear whether keratinocytes or other cell types are responsi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199710)173:1<64::AID-JCP8>

    authors: Takei T,Rivas-Gotz C,Delling CA,Koo JT,Mills I,McCarthy TL,Centrella M,Sumpio BE

    更新日期:1997-10-01 00:00:00

  • Identification of estrogen receptor mRNA and the estrogen modulation of parathyroid hormone-stimulated cyclic AMP accumulation in opossum kidney cells.

    abstract::The opossum kidney (OK) cell was used as a model to test the hypothesis that estrogen directly affects proximal renal tubular epithelial cells. To demonstrate the expression of estrogen receptor in OK cells, we developed an approach using reverse transcription and the polymerase chain reaction. Analysis of the DNA amp...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041500312

    authors: Stock JL,Coderre JA,Burke EM,Danner DB,Chipman SD,Shapiro JR

    更新日期:1992-03-01 00:00:00

  • Molecular mechanisms of nitric oxide-dependent inhibition of TPA-induced matrix metalloproteinase-9 (MMP-9) in MCF-7 cells.

    abstract::Matrix metalloproteinase-9 (MMP-9) is implicated in the invasion and metastasis of breast cancer cells. We investigated the modulatory effects of nitric oxide (NO) on the 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced MMP-9 expression in MCF-7 cells. Different chemical NO donors inhibited the extracellular content...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21658

    authors: Jespersen C,Doller A,Akool el-S,Bachmann M,Müller R,Gutwein P,Mühl H,Pfeilschifter J,Eberhardt W

    更新日期:2009-05-01 00:00:00

  • LINC00261 suppresses human colon cancer progression via sponging miR-324-3p and inactivating the Wnt/β-catenin pathway.

    abstract::Growing evidence indicates long noncoding RNAs (lncRNAs) are significant regulators in the progression of various malignant tumors including colon cancer. Dysregulation of lncRNA LINC00261 has been identified in many cancers. Investigations on LINC00261 function have revealed that LINC00261 could act as a crucial tumo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28831

    authors: Yan D,Liu W,Liu Y,Luo M

    更新日期:2019-12-01 00:00:00

  • Decrease in the basal levels of cytosolic free calcium in chondrocytes during aging in culture: possible role as differentiation-signal.

    abstract::Cell- and matrix-related parameters, which characterize the aging and differentiation process of cartilage in vivo, were measured in cultured chick epiphyseal chondrocytes during maintenance in a suspension culture for 34 days. A gradual decrease in the rates of proliferation and an increase in the size of the cells w...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041440204

    authors: Beit-Or A,Nevo Z,Kalina M,Eilam Y

    更新日期:1990-08-01 00:00:00

  • Emerging functions of p21-activated kinases in human cancer cells.

    abstract::The p21 activated kinases (Paks), an evolutionarily conserved family of serine/threonine kinases, are important for a variety of cellular functions including cell morphogenesis, motility, survival, mitosis, and angiogenesis. Paks are widely expressed in numerous tissues and are activated by growth factors and extracel...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.10167

    authors: Kumar R,Vadlamudi RK

    更新日期:2002-11-01 00:00:00

  • EGF-induced ERK-activation downstream of FAK requires rac1-NADPH oxidase.

    abstract::Reactive oxygen species (ROS) function as signaling molecules mainly by reversible oxidation of redox-sensitive target proteins. ROS can be produced in response to integrin ligation and growth factor stimulation through Rac1 and its effector protein NADPH oxidase. One of the central roles of Rac1-NADPH oxidase is acti...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22563

    authors: Flinder LI,Timofeeva OA,Rosseland CM,Wierød L,Huitfeldt HS,Skarpen E

    更新日期:2011-09-01 00:00:00

  • Sulfated malto-oligosaccharides bind to basic FGF, inhibit endothelial cell proliferation, and disrupt endothelial cell tube formation.

    abstract::The interaction of basic FGF (bFGF) with heparin, heparan sulfate and related sugars can potentiate or antagonize bFGF activity, depending on the size of the saccharide used. Oligosaccharides based on heparin structures, as small as six sugar residues, have been demonstrated to bind to bFGF and block its activity, whi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199609)168:3<657::AID-JCP1

    authors: Foxall C,Wei Z,Schaefer ME,Casabonne M,Fugedi P,Peto C,Castellot JJ Jr,Brandley BK

    更新日期:1996-09-01 00:00:00

  • Prognostic relevance of miR-137 and its liver microenvironment regulatory target gene AFM in hepatocellular carcinoma.

    abstract::MiR-137 has been identified as potential hepatocellular carcinoma (HCC) prognostic biomarkers. Highly relevant HCC prognostic biomarkers may be derived from combinations of miR-137 with its target genes involved in the regulation of liver microenvironment. This study aimed at the discovery of such a combination with i...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27855

    authors: Wei Q,Zhao L,Jiang L,Bi J,Yu Z,Zhao L,Song X,Sun M,Chen Y,Wei M

    更新日期:2019-07-01 00:00:00

  • Essential Role of CFTR in PKA-Dependent Phosphorylation, Alkalinization, and Hyperpolarization During Human Sperm Capacitation.

    abstract::Mammalian sperm require to spend a limited period of time in the female reproductive tract to become competent to fertilize in a process called capacitation. It is well established that HCO3- is essential for capacitation because it activates the atypical soluble adenylate cyclase ADCY10 leading to cAMP production, an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25634

    authors: Puga Molina LC,Pinto NA,Torres Rodríguez P,Romarowski A,Vicens Sanchez A,Visconti PE,Darszon A,Treviño CL,Buffone MG

    更新日期:2017-06-01 00:00:00

  • Adiponectin inhibits high glucose-induced angiogenesis via inhibiting autophagy in RF/6A cells.

    abstract::Adiponectin, one of the adipose-derived hormone with metabolic activity, has been reported to conversely affect angiogenesis of endothelial cells in vitro. The previous study in animal models has demonstrated that adiponectin has a protective role in retinal vascular injury following pathological stimuli. However, cli...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28659

    authors: Li R,Du J,Yao Y,Yao G,Wang X

    更新日期:2019-11-01 00:00:00

  • Release of potassium, lipids, and proteins from nonionic detergent treated chicken red blood cells.

    abstract::The plasma membrane of erythrocytes, as of other cells, is thought to act as the barrier responsible for maintaining intracellular gradients of most ions and small molecular species between the cell and its environment. Controlled application of the nonionic detergent Brij 58 effectively opened the erythrocyte plasma ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041590202

    authors: Kellermayer M,Ludány A,Miseta A,Kŏszegi T,Berta G,Bogner P,Hazlewood CF,Cameron IL,Wheatley DN

    更新日期:1994-05-01 00:00:00

  • Integrin α2β1 inhibition attenuates prostate cancer cell proliferation by cell cycle arrest, promoting apoptosis and reducing epithelial-mesenchymal transition.

    abstract::Integrin α2β1 plays an important role in cellular migration and metastasis processes associated with prostate cancer. The aim of this study was to assess whether selective inhibition of integrin α2β1 is an effective strategy to target metastatic prostate cancer cells. In this regard, we examined the effects of the inh...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.30202

    authors: Salemi Z,Azizi R,Fallahian F,Aghaei M

    更新日期:2020-12-10 00:00:00

  • Embryonal carcinoma cells (and their somatic cell hybrids) are resistant to infection by the murine parvovirus MVM, which does infect other teratocarcinoma-derived cell lines.

    abstract::Minute virus of mice (MVM), a non-defective parvovirus, has been shown to infect cultures of non-pluripotent differentiated teratocarcinoma-derived cells, but pluripotent (and "nullipotent") embryonal carcinoma cells derived from the same teratocarcinoma resist MVN infection. Somatic cell hybrids between an embryonal ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040910309

    authors: Miller RA,Ward DC,Ruddle FH

    更新日期:1977-06-01 00:00:00

  • Na+/H+ exchange is inactivated during mouse oocyte meiosis, facilitating glycine accumulation that maintains embryo cell volume.

    abstract::The coupled action of the Na(+)/H(+) exchanger NHE1 and the HCO3(-)/Cl(-) exchanger AE2 constitutes the principal mechanism for acute correction of decreased cell volume in mammalian somatic cells, while, when acting separately, they regulate intracellular pH. It was previously found that AE2 becomes inactivated durin...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24370

    authors: Zhou C,Fitzharris G,Alper SL,Baltz JM

    更新日期:2013-10-01 00:00:00

  • Incorporation of [3H]thymidine by isolated fetal myoblasts and fibroblasts in response to human placental lactogen (HPL): possible mediation of HPL action by release of immunoreactive SM-C.

    abstract::We investigated the actions of human placental lactogen (HPL) and human growth hormone (HGH) on [3H]thymidine incorporation and the release of immunoassayable somatomedin-C (SM-C) by isolated myoblasts, dermal fibroblasts, and costal cartilage explants taken from human fetuses at 11-21 weeks of gestation. The incorpor...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041250224

    authors: Hill DJ,Crace CJ,Milner RD

    更新日期:1985-11-01 00:00:00

  • Effects of amphotericin B on ion transport proteins in airway epithelial cells.

    abstract::Topical intranasal application of the antifungal Amphotericin B (AmphoB) has been shown as an effective medical treatment of chronic rhinosinusitis. Because this antibiotic forms channels in lipid membranes, we considered the possibility that it affects the properties and/or cell surface expression of ion channels/pum...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20347

    authors: Jornot L,Rochat T,Caruso A,Lacroix JS

    更新日期:2005-09-01 00:00:00

  • Adenosine receptor-mediated changes in cyclic AMP production and DNA synthesis in cultured arterial smooth muscle cells.

    abstract::The effects of adenosine and two analogs, L-phenylisopropyladenosine (L-PIA) and 5'-N-ethylcarboxamidoadenosine (NECA), on cAMP production and on platelet-derived growth factor (PDGF)-stimulated initiation of DNA synthesis in growth-arrested cultures of rat arterial smooth muscle cells (SMC) were studied. The intracel...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041240314

    authors: Jonzon B,Nilsson J,Fredholm BB

    更新日期:1985-09-01 00:00:00

  • Plasma membrane calcium ATPase 4 (PMCA4) co-ordinates calcium and nitric oxide signaling in regulating murine sperm functional activity.

    abstract::Reduced sperm motility (asthenospermia) and resulting infertility arise from deletion of the Plasma Membrane Ca2+ -ATPase 4 (Pmca4) gene which encodes the highly conserved Ca2+ efflux pump, PMCA4. This is the major Ca2+ clearance protein in murine sperm. Since the mechanism underlying asthenospermia in PMCA4's absence...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25882

    authors: Olli KE,Li K,Galileo DS,Martin-DeLeon PA

    更新日期:2018-01-01 00:00:00

  • The anti-spasticity drug baclofen alleviates collagen-induced arthritis and regulates dendritic cells.

    abstract::Baclofen is used clinically as a drug that treats spasticity, which is a syndrome characterized by excessive contraction of the muscles and hyperflexia in the central nervous system (CNS), by activating GABA(B) receptors (GABA(B)Rs). Baclofen was recently reported to desensitize chemokine receptors and to suppress inf...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24884

    authors: Huang S,Mao J,Wei B,Pei G

    更新日期:2015-07-01 00:00:00

  • Differential role of Rho GTPases in intestinal epithelial barrier regulation in vitro.

    abstract::Maintenance of intestinal epithelial barrier functions is crucial to prevent systemic contamination by microbes that penetrate from the gut lumen. GTPases of the Rho-family such as RhoA, Rac1, and Cdc42 are known to be critically involved in the regulation of intestinal epithelial barrier functions. However, it is sti...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22446

    authors: Schlegel N,Meir M,Spindler V,Germer CT,Waschke J

    更新日期:2011-05-01 00:00:00

  • Methylation of CLEC14A is associated with its expression and lung adenocarcinoma progression.

    abstract::Our main objective is probing the effect of methylation of CLEC14A on its expression and lung adenocarcinoma (LUAD) progression. Microarray analysis was utilized to screen out differentially downregulated genes with hypermethylation in LUAD tissues. The CLEC14A expression level was measured by western blot analysis an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27112

    authors: Su C,Shi K,Cheng X,Han Y,Li Y,Yu D,Liu Z

    更新日期:2019-03-01 00:00:00

  • Pyridine nucleotide metabolism in mitotic cells.

    abstract::The biosynthesis and turnover of nicotinamide adenine dinucleotide (NAD) have been examined in mitotic cells of the human culture line, D98/AH2. No significant difference in the incorporation of nicotinic acid or nicotinamide could be detected between mitotic and interphase cells. The distribution of newly-incorporate...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040860509

    authors: Elliott G,Rechsteiner M

    更新日期:1975-12-01 00:00:00

  • p120-catenin is required for regulating epidermal proliferation, differentiation, and barrier function.

    abstract::p120-catenin (p120) is an important regulator in the function and stability of E-cadherin. However, the role of p120 in the epidermis is unclear. Previous studies have shown that globally knockout of p120 caused increased epidermal proliferation but little changes in epidermal differentiation and permeability. In the ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26535

    authors: Xie Z,Tang Y,Man MQ,Shrestha C,Bikle DD

    更新日期:2018-01-01 00:00:00

  • Sensitivity of preadipose 3T3 cells to growth hormone.

    abstract::Cultured preadipose 3T3 cells are able to undergo a process of differentiation through which they are converted into adipose cells. Growth hormone induces this conversion in resting cultures but not in growing cultures. It was of interest to determine the period of cell sensitivity to the hormone and the timing of the...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041280222

    authors: Morikawa M

    更新日期:1986-08-01 00:00:00

  • mTOR and Beclin1: Two key autophagy-related molecules and their roles in myocardial ischemia/reperfusion injury.

    abstract::Autophagy is the general term of lysosomal degradation of substances in cells, which is considered the key to maintaining the normal structure and function of the heart. It also has a correlation with several heart diseases, in particular, myocardial ischemia/reperfusion (I/R) injury. At the stage of myocardial ischem...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.28125

    authors: Shi B,Ma M,Zheng Y,Pan Y,Lin X

    更新日期:2019-08-01 00:00:00

  • Effects of endothelins on hepatic stellate cell synthesis of endothelin-1 during hepatic wound healing.

    abstract::Endothelin-1 production is increased after liver injury and the subsequent wounding response. Further, endothelin-1 has prominent effects on hepatic stellate cells (key effectors of the hepatic wounding response), including on collagen synthesis, proliferation, and expression of smooth muscle proteins. We tested the h...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.10110

    authors: Shao R,Rockey DC

    更新日期:2002-06-01 00:00:00