Abstract:
:Cell- and matrix-related parameters, which characterize the aging and differentiation process of cartilage in vivo, were measured in cultured chick epiphyseal chondrocytes during maintenance in a suspension culture for 34 days. A gradual decrease in the rates of proliferation and an increase in the size of the cells were observed. Ultrastructural examination revealed increased vacuolization and appearance of glycogen-storing pools. The rate of proteoglycan synthesis gradually increased. Age-related changes in the composition of the proteoglycan consisted of an increase in the ratio of keratan sulfate/chondroitin sulfate. The results indicate that the process of aging in culture resembles maturation and differentiation of cartilage tissue in vivo. The levels of cytosolic free calcium ions ([Ca2+]i) were measured in fura-2-loaded cells during the course of aging in culture. A gradual decrease in [Ca2+]i was observed. In 5-day cultures, a value of 184 nM [Ca2+]i was measured; this value decreased to 61 nM in 34-day cultures. On the basis of the present data and the previous results, which showed that cartilage-derived growth factors caused a decrease in [Ca2+]i, concomitantly with enhancing differentiation, whereas factors which elevated [Ca2+]i caused an increase in proliferation and a decrease in proteoglycan synthesis, we suggest a model for control of chondrocyte differentiation and aging. The model suggests that the rate of differentiation may be paced by changes in steady-state levels of [Ca2+]i.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Beit-Or A,Nevo Z,Kalina M,Eilam Ydoi
10.1002/jcp.1041440204subject
Has Abstractpub_date
1990-08-01 00:00:00pages
197-203issue
2eissn
0021-9541issn
1097-4652journal_volume
144pub_type
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更新日期:1988-09-01 00:00:00
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doi:10.1002/jcp.20789
更新日期:2006-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1983-02-01 00:00:00