Heparan sulfates of mouse cells. Analysis of parent and transformed 3T3 cell lines.


:Heparan sulfate from the surface of a variety of mouse cells at different cell densities was examined by ion-exchange chromatography. The results of this analysis show that: (1) The heparan sulfate from new isolates of Swiss 3T3 cells transformed by SV40 virus (a DNA tumor virus) elutes from DEAE-cellulose at a lower ionic strength than that from the parent cell type. This finding confirms our earlier observation with an established SV40-transformed cell line (Underhill and Keller, '75) and eliminates the possibility that this change is caused by extended passage in culture. (2) For both parent and transformed 3T3 cells, the heparan sulfates from low and high density cultures were the same as judged by chromatography on DEAE-cellulose. This result demonstrates that the transformation-dependent change which we have observed is independent of cell density. (3) The heparan sulfate from Balb/c 3T3 cells transformed with Kirsten murine sarcoma virus (an RNA tumor virus) elutes from DEAE-cellulose prior to that from parent Balb/c 3T3 cells. This result extends the transformation dependent change in heparan sulfate to the Balb/c 3T3 cell line and to cells transformed with an RNA virus.


J Cell Physiol


Underhill CB,Keller JM





Has Abstract


1977-01-01 00:00:00












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    authors: Grotendorst GR,Igarashi A,Larson R,Soma Y,Charette M

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    authors: He X,Zeng H,Chen JX

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    authors: Kelley LL,Koury MJ,Bondurant MC

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    authors: Mogford JE,Tawil N,Chen A,Gies D,Xia Y,Mustoe TA

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    journal_title:Journal of cellular physiology

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    authors: Bashford CL,Alder GM,Gray MA,Micklem KJ,Taylor CC,Turek PJ,Pasternak CA

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  • Activated ERK/FOXM1 pathway by low-power laser irradiation inhibits UVB-induced senescence through down-regulating p21 expression.

    abstract::Cellular senescence is a growth-arrest program that limits cell proliferation. Low-power laser irradiation (LPLI) has been demonstrated to promote cell proliferation. However, whether LPLI can inhibit cellular senescence remains unknown. In the present study, to investigate the functional role of LPLI against skin agi...

    journal_title:Journal of cellular physiology

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    authors: Ling Q,Meng C,Chen Q,Xing D

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    authors: Stern ES,Krooth RS

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    authors: Schmid G,Kramer MP,Wesierska-Gadek J

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    journal_title:Journal of cellular physiology

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  • Elevation of a potassium current in differentiating human leukemic (HL-60) cells.

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  • Induction of plasminogen activator inhibitor 1 biosynthesis by hyperthermia.

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  • First Evidence of DAAM1 Localization During the Post-Natal Development of Rat Testis and in Mammalian Sperm.

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Rousset M,Chantret I,Darmoul D,Trugnan G,Sapin C,Green F,Swallow D,Zweibaum A

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    pub_type: 杂志文章


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