Abstract:
:In the present investigation, a hCG sensitive glycosyl-phosphatidylinositol (GPI) was isolated from cultured rat granulosa cells obtained from the ovaries of diethylstilbestrol (DES) implanted immature rats. The inositol-phosphoglycan (IPG) moiety of the GPI-lipid contains galactose, glucosamine, and myoinositol as demonstrated by metabolic labelling of granulosa cells for different time periods (5-96 h) with [3H]galactose, [3H]glucosamine, or [3H]myoinositol and treatment of the purified [3H]GPI with phosphatidylinositol-specific phospholipase C. Labelling equilibrium of the GPI-lipid was achieved after 24 h ([3H]galactose and [3H]myoinositol) or 72 h ([3H]glucosamine) incubation, whereas incorporation of other labelled carbohydrates tested ([3H]galactosamine, [3H]mannose, and [3H]sorbitol) was negligible throughout the time period studied. The glucosamine C-1 appears to be linked through a glycosidic bond to the myoinositol molecule of the IPG moiety as revealed by the generation of phosphatidylinositol (PtdIns) after nitrous acid deamination of dual labelled ([3H]glucosamine/[14C]palmitate or [3H]glucosamine/[14C]myristate) glycosyl-phosphatidylinositol. To investigate the fatty acid composition of the diacylglycerol (DAG) backbone of the GPI, granulosa cells were also labelled (5-72 hr) with [14C]linoleate, [3H]myristate, [3H]oleate, [3H]palmitate, or [3H]stearate and the radioactivity associated with the purified glycosyl-phosphatidylinositol determined. Incorporation of [3H]palmitate and [3H]myristate into the GPI-lipid peaked after 8 h and 24 h of labelling, respectively, and both fatty acids were partially released after PLA2 treatment of the dual labelled ([3H]glucosamine/[14C]palmitate or [3H]glucosamine/[14C]myristate) GPI. In parallel experiments no significant incorporation of labelled stearate, oleate, or linoleic acid into the DAG backbone of the glycosylphosphatidylinositol could be detected. Granulosa cells were also labelled with [3H]glucosamine in the presence of FSH (30 ng/ml), cholera toxin (1 microgram/ml), or the membrane permeable cAMP analog (but)2cAMP (1 mM). Time related increases in GPI-labelling were apparent after 48 h and reached a maximum level (3-, 5-, and 7-fold for FSH, CT, and (but)2cAMP, respectively) after 72 h in culture. In another set of experiments, granulosa cells were labelled for 72 h with [3H]glucosamine in the presence of (but)2cAMP (1 mM), TPA (10(-7) M), or combination thereof. The effect of treatment with the membrane permeable cAMP analog on GPI labelling was prevented in the presence of TPA, whereas no differences in [3H]GPI content could be observed in untreated granulosa cells or cells cultured in the presence of the protein kinase C-activating phorbol ester alone.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Fanjul LF,Marrero I,Estevez F,Gonzalez J,Quintana J,Santana P,Ruiz de Galarreta CMdoi
10.1002/jcp.1041550208subject
Has Abstractpub_date
1993-05-01 00:00:00pages
273-81issue
2eissn
0021-9541issn
1097-4652journal_volume
155pub_type
杂志文章abstract::Temperature-sensitive mutants have revealed a region of the herpes simplex virus 1 genome that affects both the uptake of hexose and the synthesis of heat shock proteins. Other inducers of heat-shock proteins, namely heat shock itself and arsenite, likewise induce an increased uptake of hexose. The increased uptake, l...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041280306
更新日期:1986-09-01 00:00:00
abstract::We have partially purified a mitogenic factor, termed colony stimulating factor, which is secreted from a human T lymphocytic cell line, and which stimulates in vitro formation of colonies of macrophages and granulocytes by human bone marrow cells. Colony stimulating factor (CSF) produced by cells grown in serum suppl...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041090309
更新日期:1981-12-01 00:00:00
abstract::A separation procedure has been developed for mouse splenic T and B lymphocytes which is based on their differential agglutination by wheat germ agglutinin (WGA). In the presence of 50-100 micrograms/ml of WGA, multicellular aggregates are formed which are enriched in B cells. These aggregates can be separated from mo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040990111
更新日期:1979-04-01 00:00:00
abstract::Tenascin-C, a six-armed extracellular matrix glycoprotein, is expressed in a temporally and spatially restricted pattern during carcinogenesis and invasion or metastasis of carcinoma cells in association with stromal-epithelial interactions. The human epidermoid carcinoma-derived cell lines, A431 and HEp-2, which do n...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041650104
更新日期:1995-10-01 00:00:00
abstract::Osteoclasts and macrophages share progenitors that must receive decisive lineage signals driving them into their respective differentiation routes. Macrophage colony stimulation factor M-CSF is a common factor; bone is likely the stimulus for osteoclast differentiation. To elucidate the effect of both, shared mouse bo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24702
更新日期:2015-01-01 00:00:00
abstract::AMAP1 was a GTPase-activating protein that regulates cytoskeletal structures in focal adhesions, circular dorsal ruffles, and promote cell differentiation in tumor cells. But the activation and function of AMAP1 in breast cancer remain largely unexplored. Here we show that AMAP1 was phosphorylated and translocated to ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26164
更新日期:2018-04-01 00:00:00
abstract::The protein kinase C stimulator TPA (12-O-tetradecanoyl phorbol-13-acetate) enhanced the responsiveness of adenylate cyclase to IPR (isoproterenol) and PGE1 (prostaglandin E1) in quiescent tsKSV-NRK cells at the nonpermissive 41 degrees C. Reactivating the thermolabile mitogenic/oncogenic K-ras protein in tsKSV-NRK ce...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041400302
更新日期:1989-09-01 00:00:00
abstract::Cell-extracellular matrix interaction plays a major role in maintaining the structural integrity of connective tissues and sensing changes in the biomechanical environment of cells. Collagen VI is a widely expressed non-fibrillar collagen, which regulates tissues homeostasis. The objective of the present investigation...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24518
更新日期:2014-07-01 00:00:00
abstract::During human pregnancy, the trophoblast develops from differentiation of cytotrophoblast cells into an endocrine active syncytiotrophoblast. In culture, isolated mononuclear cytotrophoblasts aggregate and then fuse to form a syncytium, reproducing the in vivo process. In this study, we examined the effect of low oxyge...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199608)168:2<346::AID-JCP1
更新日期:1996-08-01 00:00:00
abstract::Carbonic anhydrase (CAH) was localized in the mitochondria-rich cells (MRC) of 1-week-old salamander larvae gill epithelium, in both MRC and pavement cells of 6-week-old larvae, and in regenerated stems of previously amputated gills. CAH activity of the MRC was measured quantitatively using a microscope densitometric ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041300118
更新日期:1987-01-01 00:00:00
abstract::Stromal cell-derived factor-1 (SDF-1) and CXC chemokine receptor 4 (CXCR4) have been found to be tightly correlated with the progression of prostate cancer (PC). In this study, we investigated the effects of an SDF-1α/CXCR4 inhibitor, AMD3100, on cell progression and metastasis potential of human PC cells. Human PC ce...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27831
更新日期:2019-07-01 00:00:00
abstract::Evolving data suggest that marrow hematopoietic stem cells show reversible changes in homing, engraftment, and differentiation phenotype with cell cycle progression. Furthermore, marrow stem cells are a cycling population. Traditional concepts hold that the system is hierarchical, but the information on the lability o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21273
更新日期:2008-03-01 00:00:00
abstract::Resistance to tamoxifen remains a prominent conundrum in the therapy of hormone-sensitive breast cancer. Also, the molecular underpinnings leading to tamoxifen resistance remain unclear. In the present study, we utilized the Gene Expression Omnibus database to identify that SOX11 might exert a pivotal function in conf...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29629
更新日期:2020-10-01 00:00:00
abstract::Osteogenic differentiation is a complicated process that depends on various regulatory factors and signal pathways. In our research, the osteogenic differentiation capacity was analyzed by alizarin red staining, alkaline phosphatase activity, and protein levels of osteogenic differentiation markers including runt-rela...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27499
更新日期:2019-05-01 00:00:00
abstract::The concentration of ATP in cysts of the brine shrimp, Artemia salina, has been studied as a function of cyst hydration. Cysts dried over CaSO4 contain 0.02 gH2O/g cysts and 0.54 +/- 0.05 (S.D.) mumoles of ATP/g dried cysts. Addition of water up to 0.05 g/g cysts produced no net change in the level of ATP during incub...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040880205
更新日期:1976-06-01 00:00:00
abstract::Silibinin is the primary active compound in silymarin. It has been demonstrated to exert anti-carcinogenic effects and hepato-protective effects. However, the effects of silibinin on the maturation and immunostimulatory activities exhibited by dendritic cells (DCs) remain, for the most part, unknown. In this study, we...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20852
更新日期:2007-02-01 00:00:00
abstract::Previously, we found that hypoxia can deeply affect the production of cytokines in human peripheral mononuclear cells (PBMC). Here, we demonstrated that the cycle progression of hypoxic PBMC, cultured in the presence or not of a specific T cell activator such as phytohaemagglutinin (PHA), was delayed when compared wit...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199912)181:3<448::AID-JCP8
更新日期:1999-12-01 00:00:00
abstract::Human immunodeficiency virus (HIV) is one of the critical infectious agents with thousands of newly infected people worldwide. High mutational capability and rapid diversification, inhibition of humoral and cellular immune responses, and thus inability for recognition of an immunogenic region in the viral envelope by ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28280
更新日期:2019-02-18 00:00:00
abstract::Programmed cell death in mammary tissue was studied during natural weaning in lactating mice and after litter removal or milk stasis. All treatments stimulated mammary apoptosis, indicating that this process is an integral part of the tissue's involution after lactation. Induction of apoptosis was slower in natural we...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199609)168:3<559::AID-JCP8
更新日期:1996-09-01 00:00:00
abstract::The ratio of mouse to rat albumin secreted by mouse hepatoma x rat hepatoma hybrid cells is constant (of the order of 5.0) irrespective of the total amounts produced. The present results establish for seven independent hybrid clones that the coordination in the ratio of mouse to rat product applies also at the level o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041150211
更新日期:1983-05-01 00:00:00
abstract::We have analyzed the expression of Na,K-ATPase alpha subunit isoforms in the transporting ciliary processes of the human eye and in cultured cells derived from non-pigmented (NPE) and pigmented (PE) ciliary epithelium. Northern hybridization analysis shows that the mRNAs encoding all the three distinct forms of Na,K-A...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410203
更新日期:1989-11-01 00:00:00
abstract::Suppressor of cytokine signaling (SOCS)-2 regulates normal postnatal growth and its deficiency in mice causes gigantism with increased bone length and proportional enlargement in skeletal muscles. Using C2C12 mesenchymal precursor cell line as a model, we investigated a possible role of SOCS-2 in the differentiation p...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20579
更新日期:2006-05-01 00:00:00
abstract::Much research has been conducted to discover novel techniques to reverse the process of tumorigenesis and, cure already stablished malignancies. One well-stablished approach has been the use of organic compounds and naturally found agents such as melatonin whose anticancer effects have been shown in multiple studies, ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28435
更新日期:2019-08-01 00:00:00
abstract::Radiotherapy remains a prime approach to adjuvant therapies in patients with early and advanced breast cancer. In spite of therapeutic success, metastatic progression in patients undergoing therapy, limits its application. However, effective therapeutic strategies to understand the cellular and molecular machinery in ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24780
更新日期:2015-03-01 00:00:00
abstract::Trypsinized normal rat embryo fibroblasts and untrypsinized and trypsinized transformed rat fibroblasts have two orders of binding sites for bacterial glycolipid mR595. The high order sites fix 1--3 micrograms glycolipid mR595/10(5) cells and those of the low order fix about 6 microgram glycolipids mR595/10(6) cells. ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040980113
更新日期:1979-01-01 00:00:00
abstract::3-Hydroxy 3-methylglutaryl coenzyme A reductase (HMG-CoAR) is the key and rate-limiting enzyme of cholesterol biosynthetic pathway. Although HMG-CoAR activity has already been related to the differentiation of some cellular lines there are no studies that analyze the role of HMG-CoAR, and the pathway it is involved wi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21810
更新日期:2009-08-01 00:00:00
abstract::Dose-response curves for the inhibition of heterogeneous nuclear RNA (hnRNA) synthesis in HeLa cells by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB; 5-100 microM; 30 min) are biphasic and indicate the existence of two subpopulations of hnRNA molecules, one highly sensitive and the other completely resistant,...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041160106
更新日期:1983-07-01 00:00:00
abstract::Identification of cellular proteins, in addition to already known transcription factors such as NF-κB, Sp1, C-EBPβ, NFAT, ATF/CREB, and LEF-1, which interact with the HIV-1 LTR, is critical in understanding the mechanism of HIV-1 replication in monocytes/macrophages. Our studies demonstrate upregulation of pyruvate ki...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25445
更新日期:2017-03-01 00:00:00
abstract::IGF-I and its receptor IGF-IR are seen as critical effectors of muscle hypertrophy, a notion recently questioned. Using MKR transgenic mice that express a dominant negative IGF-IR only in skeletal muscle, we have examined the role of the IGF-IR signaling in differentiation and repair of muscle fibers after damage-indu...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22218
更新日期:2010-10-01 00:00:00
abstract::Copper, an essential trace element, can be toxic to some cells when present in excess. But thorough investigations into the cytotoxicity of copper and subsequent molecular mechanisms are rare, although the cytotoxicity of copper has been applied to cancer chemotherapy. The present study demonstrates that Cu(2+) inhibi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/1097-4652(200008)184:2<161::AID-JCP3>3.0.C
更新日期:2000-08-01 00:00:00