Effect of mechanical strain on the collagen VI pericellular matrix in anterior cruciate ligament fibroblasts.

Abstract:

:Cell-extracellular matrix interaction plays a major role in maintaining the structural integrity of connective tissues and sensing changes in the biomechanical environment of cells. Collagen VI is a widely expressed non-fibrillar collagen, which regulates tissues homeostasis. The objective of the present investigation was to extend our understanding of the role of collagen VI in human ACL. This study shows that collagen VI is associated both in vivo and in vitro to the cell membrane of knee ACL fibroblasts, contributing to the constitution of a microfibrillar pericellular matrix. In cultured cells the localization of collagen VI at the cell surface correlated with the expression of NG2 proteoglycan, a major collagen VI receptor. The treatment of ACL fibroblasts with anti-NG2 antibody abolished the localization of collagen VI indicating that collagen VI pericellular matrix organization in ACL fibroblasts is mainly mediated by NG2 proteoglycan. In vitro mechanical strain injury dramatically reduced the NG2 proteoglycan protein level, impaired the association of collagen VI to the cell surface, and promoted cell cycle withdrawal. Our data suggest that the injury-induced alteration of specific cell-ECM interactions may lead to a defective fibroblast self-renewal and contribute to the poor regenerative ability of ACL fibroblasts.

journal_name

J Cell Physiol

authors

Sardone F,Traina F,Tagliavini F,Pellegrini C,Merlini L,Squarzoni S,Santi S,Neri S,Faldini C,Maraldi N,Sabatelli P

doi

10.1002/jcp.24518

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

878-86

issue

7

eissn

0021-9541

issn

1097-4652

journal_volume

229

pub_type

杂志文章
  • Toll-like receptors: A pathway alluding to cancer control.

    abstract::Toll-like receptors (TLRs) are usually expressed on immune cells such as macrophages, dendritic cells, mast cells, as well as on eosinophils and some epithelial cells. They play a central role in the recognition of harmful molecules that belong to invading microorganisms or internal damaged tissues, which lead to infl...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.28879

    authors: Ayala-Cuellar AP,Cho J,Choi KC

    更新日期:2019-12-01 00:00:00

  • Permeability of human blood platelets to glycerol.

    abstract::The permeability of human platelets to glycerol was determined at 37 degrees C, 25 degrees C, and 0 degrees C from the rate of change of cell volume after abrupt addition of 0.5 mol/liter glycerol in phosphate-buffered saline. Intracellular water volume was measured employing both tritiated water and a photometric met...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041280118

    authors: Armitage WJ

    更新日期:1986-07-01 00:00:00

  • Culture-induced increase in acidic and basic fibroblast growth factor activities and their association with the nuclei of vascular endothelial and smooth muscle cells.

    abstract::The activity of acidic and basic fibroblast growth factor-like mitogens (aFGF, bFGF) extracted from cultured bovine aortic endothelial (BAEC) and rat aortic smooth muscle cells (SMC) was compared with that of freshly isolated cells from the same tissues. Extracts of subendothelial extracellular matrix (ECM) and cell l...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041470223

    authors: Speir E,Sasse J,Shrivastav S,Casscells W

    更新日期:1991-05-01 00:00:00

  • Porphyromonas gingivalis lipopolysaccharide induces shedding of syndecan-1 expressed by gingival epithelial cells.

    abstract::Syndecans are constitutively shed from growing epithelial cells as the part of normal cell surface turnover. However, increased serum levels of the soluble syndecan ectodomain have been reported to occur during bacterial infections. The aim of this study was to evaluate the potential of lipopolysaccharide (LPS) from t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20287

    authors: Andrian E,Grenier D,Rouabhia M

    更新日期:2005-07-01 00:00:00

  • Protease nexins: cell-secreted proteins that mediate the binding, internalization, and degradation of regulatory serine proteases.

    abstract::The protease nexins (PN-I, Mr approximately 38,000; PN-II, Mr approximately 95,000; and PN-III, Mr approximately 31,000) are recently described cell-secreted proteins that selectively link to regulatory serine proteases in the extracellular environment and mediate their cellular binding, internalization, and degradati...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041170314

    authors: Knauer DJ,Thompson JA,Cunningham DD

    更新日期:1983-12-01 00:00:00

  • Relationship of heat labile glucose-6-phosphate dehydrogenase and multiple molecular forms of the enzyme in senescent human fibroblasts.

    abstract::Human fibroblasts derived from newborn foreskin and designated CF-3 were assayed for heat labile glucose-6-phosphate dehydrogenase (G6PD) when they had grown to confluency, as well as when they were arrested in an essentially nonmitotic state. Under both culture conditions there was an increase in heat labile G6PD (up...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040930108

    authors: Duncan MR,Dell'orco RT,Guthrie PL

    更新日期:1977-10-01 00:00:00

  • Antibodies Against Mimotopes of Simian Virus 40 Large T Antigen, the Oncoprotein, in Serum Samples From Elderly Healthy Subjects.

    abstract::Simian Virus 40 (SV40), a monkey polyomavirus, was administered to human populations by early anti-poliomylitis vaccines contaminated by this small DNA tumor virus. Data on SV40 infection in humans remain controversial. Elderly subjects represent an interesting cohort to investigate, because they were not immunized wi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25405

    authors: Mazzoni E,Guerra G,Casali MV,Pietrobon S,Bononi I,Puozzo A,Tagliapietra A,Nocini PF,Tognon M,Martini F

    更新日期:2017-01-01 00:00:00

  • Altered methionine metabolism occurs in all members of a set of diverse human tumor cell lines.

    abstract::Methionine dependence is a metabolic defect found thus far only in transformed and malignant cells. The defect is manifested as the inability of cells to grow in media in which methionine (Met) is replaced by its immediate precursor homocysteine (Hcy). We have termed this Met- Hcy+ media. We demonstrate here that meth...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041190106

    authors: Stern PH,Wallace CD,Hoffman RM

    更新日期:1984-04-01 00:00:00

  • Induction of heat-shock response and alterations of protein phosphorylation by a novel topoisomerase II inhibitor, withangulatin A, in 9L rat brain tumor cells.

    abstract::Withangulatin A is a newly identified in vitro topoisomerase II inhibitor isolated from the Chinese antitumor herb Physalis angulata. In vivo, it was found to be cytotoxic, capable of suppressing general protein synthesis and of inducing the synthesis of a small set of proteins including those generated by heat-shock ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041490110

    authors: Lee WC,Lin KY,Chen CM,Chen ZT,Liu HJ,Lai YK

    更新日期:1991-10-01 00:00:00

  • Association between AXL, Hippo Transducers, and Survival Outcomes in Male Breast Cancer.

    abstract::Male breast cancer (MBC) is an uncommon malignancy. We have previously reported that the expression of the Hippo transducers TAZ/YAP and their target CTGF was associated with inferior survival in MBC patients. Preclinical evidence demonstrated that Axl is a transcriptional target of TAZ/YAP. Thus, we herein assessed A...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25745

    authors: Di Benedetto A,Mottolese M,Sperati F,Ercolani C,Di Lauro L,Pizzuti L,Vici P,Terrenato I,Shaaban AM,Humphries MP,Sundara-Rajan S,Barba M,Speirs V,De Maria R,Maugeri-Saccà M

    更新日期:2017-08-01 00:00:00

  • Role of polyamines in the stimulation of synthesis and secretion of plasminogen activator from bovine aortic endothelial cells.

    abstract::The effects of the polyamines putrescine (PUT), spermidine (SPD), and spermidine (SPM) on the secretion of plasminogen activator (PA) and plasminogen activator inhibitor (PAI) were evaluated using cultured bovine aortic endothelial cells. All three polyamines enhanced PA secretion in a time- and dose-dependent manner,...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041370124

    authors: Kuo BS,Korner G,Dryjski M,Bjornsson TD

    更新日期:1988-10-01 00:00:00

  • Codon usage pattern and evolutionary forces of mitochondrial ND genes among orders of class Amphibia.

    abstract::In this study, we used a bioinformatics approach to analyze the nucleotide composition and pattern of synonymous codon usage in mitochondrial ND genes in three amphibian groups, that is, orders Anura, Caudata, and Gymnophiona to identify the commonality and the differences of codon usage as no research work was report...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.30050

    authors: Barbhuiya PA,Uddin A,Chakraborty S

    更新日期:2020-09-22 00:00:00

  • Three forms of rat basic fibroblast growth factor are made from a single mRNA and localize to the nucleus.

    abstract::The molecular weight of rat basic fibroblast growth factor is predicted to be 18 kDa when the amino acid sequence is read from the single AUG initiation codon found in the cDNA. DNA sequencing upstream of this AUG codon indicated, however, that there was an extended open reading frame. In vitro translation of the rat ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041480204

    authors: Powell PP,Klagsbrun M

    更新日期:1991-08-01 00:00:00

  • Isolation and characterization of a cloned growth factor dependent macrophage cell line, BAC1.2F5.

    abstract::The SV40 transformed murine macrophage cell line, BAC1, proliferates in response to the colony stimulating factor, CSF-1 (Schwarzbaum et al., J. Immunol., 132:1158, 1984). In order to obtain a cell line suitable for biochemical and genetic studies of CSF-1 signal transduction, clones of BAC1 were established. Clones r...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041300316

    authors: Morgan C,Pollard JW,Stanley ER

    更新日期:1987-03-01 00:00:00

  • Heparin and heparan sulfate binding sites on B-16 melanoma cells.

    abstract::We have reported previously that the production of a tumor cell factor that stimulates synthesis of fibroblast collagenase is influenced by a fibroblast-deposited matrix component, possibly heparan sulfate-proteoglycan. In this study, binding sites for heparin and heparan sulfate on mouse B-16 melanoma cells have been...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041360119

    authors: Biswas C

    更新日期:1988-07-01 00:00:00

  • Functional measurement of local proteolytic activity in living cells of invasive and non-invasive tumors.

    abstract::Proteolytic cleavage of extracellular matrix (ECM) and disruption of tissue architecture are fundamental features of tumor cell invasion. The proteolytic activity is focused in close proximity to the tumor cells. Here, we describe the possibility to quantify local proteolytic activity in the microenvironment of larger...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20168

    authors: Ludwig T,Püttmann S,Bertram H,Tatenhorst L,Paulus W,Oberleithner H,Senner V

    更新日期:2005-03-01 00:00:00

  • Epidermal growth factor receptor-dependent PI3K-activation promotes restitution of wounded human gastric epithelial monolayers.

    abstract::Restitution is a crucial event during the healing of superficial injury of the gastric mucosa involving epithelial cell sheet movement into the damaged area. We demonstrated that growth factors promote the restitution of human gastric epithelial cells. However, the intracellular signaling pathways that transmit extrac...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21239

    authors: Tétreault MP,Chailler P,Beaulieu JF,Rivard N,Ménard D

    更新日期:2008-02-01 00:00:00

  • Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity.

    abstract::The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21289

    authors: Ding Y,Brackenbury WJ,Onganer PU,Montano X,Porter LM,Bates LF,Djamgoz MB

    更新日期:2008-04-01 00:00:00

  • Expression of P2 Purinergic Receptors in Mesenchymal Stem Cells and Their Roles in Extracellular Nucleotide Regulation of Cell Functions.

    abstract::Extracellular ATP and other nucleotides induce autocrine and/or paracrine purinergic signalling via activation of the P2 receptors on the cell surface, which represents one of the most common signalling mechanisms. Mesenchymal stem cells (MSC) are a type of multipotent adult stem cells that have many promising applica...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.25484

    authors: Jiang LH,Hao Y,Mousawi F,Peng H,Yang X

    更新日期:2017-02-01 00:00:00

  • FOXE1 supports the tumor promotion of Gli2 on papillary thyroid carcinoma by the Wnt/β-catenin pathway.

    abstract::Papillary thyroid carcinoma (PTC) is a common malignancy in thyroid tissue. However, the molecular mechanism of PTC tumor progression remains unknown. The hedgehog (Hh) pathway is thought to play a key role during PTC development. Here we investigate the effects of glioma-associated oncogene protein-2 (Gli2), an impor...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28399

    authors: Ma J,Huang X,Li Z,Shen Y,Lai J,Su Q,Zhao J,Xu J

    更新日期:2019-08-01 00:00:00

  • M-CSF priming of osteoclast precursors can cause osteoclastogenesis-insensitivity, which can be prevented and overcome on bone.

    abstract::Osteoclasts and macrophages share progenitors that must receive decisive lineage signals driving them into their respective differentiation routes. Macrophage colony stimulation factor M-CSF is a common factor; bone is likely the stimulus for osteoclast differentiation. To elucidate the effect of both, shared mouse bo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24702

    authors: De Vries TJ,Schoenmaker T,Aerts D,Grevers LC,Souza PP,Nazmi K,van de Wiel M,Ylstra B,Lent PL,Leenen PJ,Everts V

    更新日期:2015-01-01 00:00:00

  • Apelin/APJ system: A novel promising target for neurodegenerative diseases.

    abstract::Neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), and Huntington's disease (HD), are incurable diseases characterized by progressive loss of cognitive or motor function, which construct a serious threat to the life quality of aging populations and their life spans. Apelin is an ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.29001

    authors: Luo H,Han L,Xu J

    更新日期:2020-02-01 00:00:00

  • Evidence for the impact of BAG3 on electrophysiological activity of primary culture of neonatal cardiomyocytes.

    abstract::Homeostasis of proteins involved in contractility of individual cardiomyocytes and those coupling adjacent cells is of critical importance as any abnormalities in cardiac electrical conduction may result in cardiac irregular activity and heart failure. Bcl2-associated athanogene 3 (BAG3) is a stress-induced protein wh...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28471

    authors: Tahrir FG,Gordon J,Feldman AM,Cheung J,Khalili K,Mohseni Ahooyi T

    更新日期:2019-08-01 00:00:00

  • Effect of an alkaline shift on induction of the heat shock response in human fibroblasts.

    abstract::The simultaneous exposure of WI-38 human fibroblasts (HF) to a heat shock (45 degrees C, 30 min) and an alkaline shift (> or = pH 8.0) in the incubation medium increased and extended the expression of heat shock proteins (hsps). Hsp70 was the most prominent inducible hsp synthesized during the recovery phase after the...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041620304

    authors: Petronini PG,Alfieri R,Campanini C,Borghetti AF

    更新日期:1995-03-01 00:00:00

  • Development of the LYVE-1 gene with an acidic-amino-acid-rich (AAAR) domain in evolution is associated with acquisition of lymph nodes and efficient adaptive immunity.

    abstract::CRSBP-1 (mammalian LYVE-1) is a membrane glycoprotein highly expressed in lymphatic endothelial cells (LECs). It has multiple ligands, including hyaluronic acid (HA) and growth factors/cytokines (e.g., PDGF-BB and VEGF-A) containing CRS motifs (clusters of basic amino-acid residues). The ligand binding activities are ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26159

    authors: Huang SS,Li YW,Wu JL,Johnson FE,Huang JS

    更新日期:2018-04-01 00:00:00

  • Human articular chondrocytes suppress in vitro proliferation of anti-CD3 activated peripheral blood mononuclear cells.

    abstract:OBJECTIVE:To investigate whether mature human articular chondrocytes (AC) exhibit an antiproliferative effect on activated peripheral blood mononuclear cells (PBMC) and to compare this effect with other cells of mesenchymal origin. METHODS:AC from healthy cadaveric cartilage were grown for different passages, in the a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20789

    authors: Bocelli-Tyndall C,Barbero A,Candrian C,Ceredig R,Tyndall A,Martin I

    更新日期:2006-12-01 00:00:00

  • Effects of interaction between estradiol-17 beta and progesterone on the proliferation of cloned breast tumor cells (MCF-7 and T47D).

    abstract::We have previously reported that the proliferation of cloned MCF-7 and T47D human mammary tumor cells can be inhibited by increasing concentrations of charcoal-dextran stripped female human serum (CDFHS). The maximal proliferation rate was restored by the addition of 3 X 10(-11) M estradiol-17 beta to the culture medi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041240305

    authors: Schatz RW,Soto AM,Sonnenschein C

    更新日期:1985-09-01 00:00:00

  • Upregulation of microRNA-218 reduces cardiac microvascular endothelial cells injury induced by coronary artery disease through the inhibition of HMGB1.

    abstract::This study is performed to examine the impacts of microRNA-218 (miR-218) on cardiac microvascular endothelial cells (CMECs) injury induced by coronary artery disease (CAD). Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) was applied for detecting miR-218 expression in serum of patients with CAD ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29214

    authors: Gao W,Cui H,Li Q,Zhong H,Yu J,Li P,He X

    更新日期:2020-03-01 00:00:00

  • Primary colonospheres maintain stem cell-like key features after cryopreservation.

    abstract::The development of efficient and repeatable protocols for biobanking and prolonged storage of cancer stem cells (CSCs), with minimum alterations in biological function, is valuable and desired, particularly for retrospective analysis and clinical applications. In particular, data regarding the effect of cryopreservatio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29150

    authors: Eini L,Naseri M,Karimi-Busheri F,Bozorgmehr M,Ghods R,Madjd Z

    更新日期:2020-03-01 00:00:00

  • Reverse transformation of vole cells transformed by avian sarcoma virus containing the src gene.

    abstract::Vole cells transformed by avian sarcoma virus carrying the src gene lose their fibroblastic morphology, the organized cytoskeletal system of the normal fibroblastic cell, the typical fibronectin deposit around the cell membrane, and the ability to shut off multiplication when suspended in liquid medium. All of these t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041070312

    authors: Puck TT,Erikson RL,Meek WD,Nielson SE

    更新日期:1981-06-01 00:00:00