Abstract:
:The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. Similar treatment with EGF significantly and dose-dependently enhanced the cells' migration through Transwell filters. Both the patch clamp recordings and the migration assay suggested that endogenous EGF played a similar role. Importantly, co-application of EGF and tetrodotoxin, a highly selective VGSC blocker, abolished 65% of the potentiating effect of EGF. It is suggested that a significant portion of the EGF-induced enhancement of migration occurred via VGSC activity.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Ding Y,Brackenbury WJ,Onganer PU,Montano X,Porter LM,Bates LF,Djamgoz MBdoi
10.1002/jcp.21289subject
Has Abstractpub_date
2008-04-01 00:00:00pages
77-81issue
1eissn
0021-9541issn
1097-4652journal_volume
215pub_type
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abstract::The synthesis and turnover of metabolically labeled proteoglycans from medium, cell layer, and substratum-associated compartments were characterized in four cell lines of fibroblastic origin, including a fibrosarcoma line, and in the murine melanoma cell type, B16.F10. Substantial differences were apparent between the...
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