Abstract:
:Copper, an essential trace element, can be toxic to some cells when present in excess. But thorough investigations into the cytotoxicity of copper and subsequent molecular mechanisms are rare, although the cytotoxicity of copper has been applied to cancer chemotherapy. The present study demonstrates that Cu(2+) inhibits [(3)H] thymidine incorporation in mouse pro-B cell line BA/F3beta and induces apoptosis. Apoptosis was mainly judged by morphology of cells, quantification of subdiploid DNA contents by flow cytometry, and detection of DNA fragmentation by gel electrophoresis. The apoptotic effect is dose and time dependent. Western blotting shows Bax is upregulated by Cu(2+). Bcl-2 overexpression can partially inhibit this apoptosis. Moreover, Cu(2+) increases the production of reactive oxygen species (ROS) in a dose-dependent manner. The antioxidant N-acetylcysteine (NAC) not only significantly inhibited copper-induced apoptosis but also totally blocked generation of ROS, while Bcl-2 overexpression has no effect on the generation of ROS. Furthermore, our results show that NFkappaB is downregulated by Cu(2+). Bcl-2 overexpression or NAC can sustain the activity of NFkappaB. These data indicate that Cu(2+) might induce apoptosis in BA/F3beta cells via upregulation of Bax and ROS and subsequent inactivation of NFkappaB.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Zhai Q,Ji H,Zheng Z,Yu X,Sun L,Liu Xdoi
10.1002/1097-4652(200008)184:2<161::AID-JCP3>3.0.Ckeywords:
subject
Has Abstractpub_date
2000-08-01 00:00:00pages
161-70issue
2eissn
0021-9541issn
1097-4652pii
10.1002/1097-4652(200008)184:2<161::AID-JCP3>3.0.Cjournal_volume
184pub_type
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