Effect of tumor-associated mutant E-cadherin variants with defects in exons 8 or 9 on matrix metalloproteinase 3.


:Tumor progression is characterized by loss of cell adhesion and increase of invasion and metastasis. The cell adhesion molecule E-cadherin is frequently down-regulated or mutated in tumors. In addition to down-regulation of cell adhesion, degradation of the extracellular matrix by matrix metalloproteinases is necessary for tumor cell spread. To investigate a possible link between E-cadherin and matrix metalloproteinase 3 (MMP-3), we examined expression of MMP-3 in human MDA-MB-435S cells transfected with wild-type (wt) or three different tumor-associated mutant E-cadherin variants with alterations in exons 8 or 9, originally identified in gastric carcinoma patients. In the presence of wt E-cadherin, the MMP-3 protein level was decreased in cellular lysates and in the supernatant where a secreted form of the protein is detectable. Down-regulation of MMP-3 was not found in MDA-MB-435S transfectants expressing mutant E-cadherin variants which indicates that E-cadherin mutations interfere with the MMP-3 suppressing function of E-cadherin. The mechanism of regulation of MMP-3 by E-cadherin is presently not clear. We have previously found that cell motility is enhanced by expression of the mutant E-cadherin variants used in this study. Here, we found that application of the synthetic inhibitor of MMP-3 NNGH and small interfering RNA (siRNA) directed against MMP-3 reduce mutant E-cadherin-enhanced cell motility. Taken together, our results point to a functional link between MMP-3 and E-cadherin. MMP-3 is differentially regulated by expression of wt or mutant E-cadherin. On the other hand, MMP-3 plays a role in the enhancement of cell motility by mutant E-cadherin. Both observations may be highly relevant for tumor progression since they concern degradation of the extracellular matrix and tumor cell spread.


J Cell Physiol


Fuchs M,Hermannstädter C,Specht K,Knyazev P,Ullrich A,Rosivatz E,Busch R,Hutzler P,Höfler H,Luber B





Has Abstract


2005-03-01 00:00:00












  • Thymidylate synthetase and dihydrofolic acid reductase in the stimulated human lymphocyte.

    abstract::The phytohemagglutinin (PHA)-stimulated human lymphocytes demonstrated trace or no activity (dihydro) folate acid reductase using three methods including a radioassay, but demonstrated ample activity of thymidylate synthetase. This was true regardless of the day of harvest, (first through seventh) of the stimulated ly...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Haurani FI,Kardinal CG,Biermann WA

    更新日期:1978-04-01 00:00:00

  • Measurements of contraction latencies to mechanical and electrical stimulation of the protozoan, Spirostomum ambiguum.

    abstract::Measurements made on contraction latencies in Spirostomun suggest that mechanical stimulation causes contractions to be initiated by the release of small amounts of calcium from a store tightly coupled to the contractile apparatus. Contraction to electrical stimulation appears to result from the gross electrophoretic ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hamilton TC,Osborn D

    更新日期:1977-06-01 00:00:00

  • Protein kinase C can inhibit TRPC3 channels indirectly via stimulating protein kinase G.

    abstract::There are two known phosphorylation-mediated inactivation mechanisms for TRPC3 channels. Protein kinase G (PKG) inactivates TRPC3 by direct phosphorylation on Thr-11 and Ser-263 of the TRPC3 proteins, and protein kinase C (PKC) inactivates TRPC3 by phosphorylation on Ser-712. In the present study, we explored the rela...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kwan HY,Huang Y,Yao X

    更新日期:2006-05-01 00:00:00

  • MMH cells: An in vitro model for the study of retinol-binding protein secretion regulated by retinol.

    abstract::The untransformed stable cell line Met murine hepatocytes (MMH), generated from liver explants of transgenic mice expressing a constitutively active truncated form of the human hepatocyte growth factor receptor (cyto-Met), represents an innovative tool for in vitro studies of liver function. In the present report, we ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bellovino D,Lanyau Y,Garaguso I,Amicone L,Cavallari C,Tripodi M,Gaetani S

    更新日期:1999-10-01 00:00:00

  • M-CSF priming of osteoclast precursors can cause osteoclastogenesis-insensitivity, which can be prevented and overcome on bone.

    abstract::Osteoclasts and macrophages share progenitors that must receive decisive lineage signals driving them into their respective differentiation routes. Macrophage colony stimulation factor M-CSF is a common factor; bone is likely the stimulus for osteoclast differentiation. To elucidate the effect of both, shared mouse bo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: De Vries TJ,Schoenmaker T,Aerts D,Grevers LC,Souza PP,Nazmi K,van de Wiel M,Ylstra B,Lent PL,Leenen PJ,Everts V

    更新日期:2015-01-01 00:00:00

  • Calcium induces differentiation of primary human salivary acinar cells.

    abstract::Cultivation of human parotid glands in serum-free medium (Ca(2+) concentration, 0.2 mM) with growth supplements resulted in isolation of a homogeneous population of epithelial cells without any mesenchymal cells. The isolated cells showed an undifferentiated phenotype with scant cytoplasmic organelles, and low levels ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hiraki A,Shirasuna K,Ikari T,Shinohara M,Garrod DR

    更新日期:2002-10-01 00:00:00

  • Oxygen-induced transcriptional dynamics in human osteoblasts are most prominent at the onset of mineralization.

    abstract::Oxygen tension plays an important role in the regulation of cellular processes. During hematopoietic stem cell (HSC) differentiation, HSCs migrate from one stem cell niche to the next, each with a different oxygen tension that determines which signaling pathways are on and off, determining the differentiation stage of...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Nicolaije C,van de Peppel J,van Leeuwen JP

    更新日期:2013-09-01 00:00:00

  • The induction of tuftelin expression in PC12 cell line during hypoxia and NGF-induced differentiation.

    abstract::The tuftelin protein isoforms undergo post-translation modifications, and are ubiquitously expressed in various tissues in embryos, adults, and tumors. Developmental and pathological studies suggested an apparent correlation between oxygen deprivation and tuftelin expression. The aim of the study was therefore to inve...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Leiser Y,Silverstein N,Blumenfeld A,Shilo D,Haze A,Rosenfeld E,Shay B,Tabakman R,Lecht S,Lazarovici P,Deutsch D

    更新日期:2011-01-01 00:00:00

  • Heparan sulfates of mouse cells. Analysis of parent and transformed 3T3 cell lines.

    abstract::Heparan sulfate from the surface of a variety of mouse cells at different cell densities was examined by ion-exchange chromatography. The results of this analysis show that: (1) The heparan sulfate from new isolates of Swiss 3T3 cells transformed by SV40 virus (a DNA tumor virus) elutes from DEAE-cellulose at a lower ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Underhill CB,Keller JM

    更新日期:1977-01-01 00:00:00

  • Bombesin enhancement of cAMP accumulation in Swiss 3T3 cells: evidence of a dual mechanism of action.

    abstract::Addition of bombesin in the presence of either forskolin or cholera toxin caused a marked (4-6 fold) enhancement of cAMP accumulation in Swiss 3T3 cells. This effect was time and concentration dependent, induced by various bombesin-like peptides and blocked by a bombesin antagonist. Enhancement of cAMP accumulation by...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Millar JB,Rozengurt E

    更新日期:1988-11-01 00:00:00

  • miR-139-5p reverses stemness maintenance and metastasis of colon cancer stem-like cells by targeting E2-2.

    abstract::Colon cancer stem cells (CCSCs) stand for a critical subpopulation of colon cancer cells that possess self-renewal and multilineage differentiation potentials and drive tumorigenicity. Due to their impact on treatment tolerance, CCSCs have been a hot research topic in the past few years. We have previously reported th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ma X,Liu J,Li J,Li Y,Le VM,Li S,Liang X,Liu L,Liu J

    更新日期:2019-12-01 00:00:00

  • Tumor suppressors Sav/Scrib and oncogene Ras regulate stem-cell transformation in adult Drosophila malpighian tubules.

    abstract::An increasing body of evidence suggests that tumors might originate from a few transformed cells that share many properties with normal stem cells. However, it remains unclear how normal stem cells are transformed into cancer stem cells (CSCs). Here, we demonstrated that mutations causing the loss of tumor suppressor ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zeng X,Singh SR,Hou D,Hou SX

    更新日期:2010-09-01 00:00:00

  • Tiliroside is a new potential therapeutic drug for osteoporosis in mice.

    abstract::Osteoporosis is a class of metabolic bone disease caused by complexed ramifications. Overactivation of osteoclasts due to a sudden decreased estrogen level plays a pivotal role for postmenopausal women suffering from osteoporosis. Therefore, inhibiting osteoclast formation and function has become a major direction for...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Li K,Xiao Y,Wang Z,Fu F,Shao S,Song F,Zhao J,Lin X,Liu Q,Xu J

    更新日期:2019-02-27 00:00:00

  • Precursor of mast cells fixed in the skin of mice.

    abstract::The presence and origin of mast-cell precursors fixed in the skin tissue of mice were investigated. Giant granules of beige (C57BL/6-bh'/bg', Chediak-Higashi syndrome) mice were used to distinguish different populations of mast cells. Pieces of the skin were grafted from the intact WBB6F1 (WB X C57BL/6)F1-+/+ mice int...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Matsuda H,Kitamura Y,Sonoda T,Imori T

    更新日期:1981-09-01 00:00:00

  • Proteasome inhibitor MG132 induces BAG3 expression through activation of heat shock factor 1.

    abstract::BAG3 protein, a member of the BAG co-chaperones family, sustains cell survival in a variety of normal and neoplastic cell types, via its interaction with a variety of partners, such as the heat shock protein (HSP) 70, Bcl-2, Raf-1 and others. Expression of BAG3 is induced by some stressful stimuli, such as heat shock,...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Du ZX,Zhang HY,Meng X,Gao YY,Zou RL,Liu BQ,Guan Y,Wang HQ

    更新日期:2009-03-01 00:00:00

  • Androgen receptor-related micro RNAs in prostate cancer and their role in antiandrogen drug resistance.

    abstract::Prostate cancer (PCa) is one of the most common cancers and the fifth most common reason for cancer deaths in the males. Surgical castration combined with androgen deprivation therapy, antiandrogens, and androgen synthesis inhibitors is the current therapeutic modalities for PCa. These strategies inhibit androgen synt...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Rezaei S,Mahjoubin Tehran M,Sahebkar A,Jalili A,Aghaee-Bakhtiari SH

    更新日期:2020-04-01 00:00:00

  • Exosomes are the novel players involved in the beneficial effects of exercise on type 2 diabetes.

    abstract::Exosomes contain regulatory signals such as lipids, proteins, and nucleic acids which can be transferred to adjacent or remote cells to mediate cell-to-cell communication. Exercise is a positive lifestyle for metabolic health and a nonpharmacological treatment of insulin resistance and metabolic diseases. Moreover, ex...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Li G,Liu H,Ma C,Chen Y,Wang J,Yang Y

    更新日期:2019-02-12 00:00:00

  • Lipid A, the active part of bacterial endotoxins in inducing serum colony stimulating activity and proliferation of splenic granulocyte/macrophage progenitor cells.

    abstract::An analysis of which component of lipopolysaccharides (LPS), the lipid or the polysaccharide (PS), is active in stimulating the murine granulopoietic system has been performed. LPS with different structures, isolated from different mutant strains of Salmonella and chemical degradation products of lipopolysaccharides h...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Apte RN,Galanos C,Pluznik DH

    更新日期:1976-01-01 00:00:00

  • LncRNA LINC01857 promotes growth, migration, and invasion of glioma by modulating miR-1281/TRIM65 axis.

    abstract::The great importance of long noncoding RNAs (lncRNAs) has been acknowledged in tumorigenesis gradually. LncRNA LINC01857 is a novel lncRNA and has been reported to promote breast cancer progression. However, the biological roles of LINC01857 in glioma are not explored. In the present research, LINC01857 levels were fo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hu G,Liu N,Wang H,Wang Y,Guo Z

    更新日期:2019-12-01 00:00:00

  • Coordinated Dynamics of RNA Splicing Speckles in the Nucleus.

    abstract::Despite being densely packed with chromatin, nuclear bodies and a nucleoskeletal network, the nucleus is a remarkably dynamic organelle. Chromatin loops form and relax, RNA transcripts and transcription factors move diffusively, and nuclear bodies move. We show here that RNA splicing speckled domains (splicing speckle...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang Q,Kota KP,Alam SG,Nickerson JA,Dickinson RB,Lele TP

    更新日期:2016-06-01 00:00:00

  • Elevated fibroblast growth factor-2 increases tumor necrosis factor-alpha induced endothelial cell death in high glucose.

    abstract::Glucose and tumor necrosis factor-alpha (TNFalpha) concentrations are elevated in diabetes. Both of these factors correlate with diabetic vasculopathy and endothelial cell apoptosis, yet their combined effects have not been measured. We have previously shown that the angiogenic growth factor fibroblast growth factor-2...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Clyne AM,Zhu H,Edelman ER

    更新日期:2008-10-01 00:00:00

  • The effect of amino acids on the temperature sensitive phenotype of the mammalian leucyl-tRNA synthetase mutant tsHl and its revertants.

    abstract::The temperature sensitive leucyl-tRNA synthetase mutant tsHl and two revertants have been compared to the parental Chinese hamster ovary cells with respect to the effects of amino acid concentrations in the medium on growth. Elevating the leucine concentration 30- or 100-fold allowed tsHl to grow exponentially at 38.5...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Molnar SJ,Rauth AM

    更新日期:1979-02-01 00:00:00

  • RUNX1 and RUNX2 transcription factors function in opposing roles to regulate breast cancer stem cells.

    abstract::Breast cancer stem cells (BCSCs) are competent to initiate tumor formation and growth and refractory to conventional therapies. Consequently BCSCs are implicated in tumor recurrence. Many signaling cascades associated with BCSCs are critical for epithelial-to-mesenchymal transition (EMT). We developed a model system t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Fritz AJ,Hong D,Boyd J,Kost J,Finstaad KH,Fitzgerald MP,Hanna S,Abuarqoub AH,Malik M,Bushweller J,Tye C,Ghule P,Gordon J,Frietze S,Zaidi SK,Lian JB,Stein JL,Stein GS

    更新日期:2020-10-01 00:00:00

  • Modulatory effects of statins on the autophagy: A therapeutic perspective.

    abstract::Autophagy is considered as an important mechanism for maintaining homeostasis and responsible for the degradation of superfluous or potentially toxic components and organelles. Autophagy impairment is associated with a number of pathological conditions, such as aging, neurological disorders, cancer, and infection. Aut...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Ashrafizadeh M,Ahmadi Z,Farkhondeh T,Samarghandian S

    更新日期:2020-04-01 00:00:00

  • Hemin promotes proliferation and differentiation of endothelial progenitor cells via activation of AKT and ERK.

    abstract::Increased neovascularization is commonly observed in hemorrhagic plaques and associated with rupture of atherosclerotic lesions. This study aims to investigate whether hemin accumulated at the site of hematoma promotes neovascularization through affecting the growth and function of endothelial progenitor cells (EPCs) ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wang JY,Lee YT,Chang PF,Chau LY

    更新日期:2009-06-01 00:00:00

  • Adhesive substrates for fibronectin.

    abstract::In order to promote cell attachment, fibronectin must first undergo activation by a suitable substrate. In this study, 52 materials have been surveyed for their ability a) to bind fibronectin, b) to activate the cell-adhesive property of fibronectin, and c) to support the growth of cells. Many plastics, polysaccharide...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Klebe RJ,Bentley KL,Schoen RC

    更新日期:1981-12-01 00:00:00

  • Melatonin-induced increase of lipid droplets accumulation and in vitro maturation in porcine oocytes is mediated by mitochondrial quiescence.

    abstract::Melatonin, the major pineal secretory product, has a significant impact on the female reproductive system. Recently, the beneficial effects of melatonin on mammalian oocyte maturation and embryonic development have drawn increased attention. However, the exact underlying mechanisms remain to be fully elucidated. This ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: He B,Yin C,Gong Y,Liu J,Guo H,Zhao R

    更新日期:2018-01-01 00:00:00

  • Fibronectin promotes the elongation of microvessels during angiogenesis in vitro.

    abstract::Fibronectin is a component of the extracellular matrix of developing microvessels whose role in angiogenesis is poorly understood. This study evaluated the effect of plasma fibronectin on angiogenesis in serum-free collagen gel culture of rat aorta. Aortic explants embedded in collagen gels generated microvascular out...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Nicosia RF,Bonanno E,Smith M

    更新日期:1993-03-01 00:00:00

  • Endothelial cells in culture produce a vasoconstrictor substance.

    abstract::We report that cultured vascular endothelial cells release into the culture medium a vasoconstrictor peptide, a substance we call an endothelium-derived constricting factor (EDCF). Conditioned medium from cultured bovine aortic and pulmonary artery endothelial cells caused sustained, dose-dependent isometric constrict...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: O'Brien RF,Robbins RJ,McMurtry IF

    更新日期:1987-08-01 00:00:00

  • Inhibition of late endosome-lysosome fusion: studies on the mechanism by which isotonic-K+ buffers alter intracellular ligand movement.

    abstract::Incubation of alveolar macrophages or hepatocytes in media in which Na+ is replaced by K+ ("isotonic-K buffer") inhibited the movement of internalized ligand from late endosomes to lysosomes (Ward et al.: Journal of Cell Biology 110:1013-1022, 1990). In this study we investigate the mechanism responsible for the isoto...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ward DM,Hackenyos DP,Davis-Kaplan S,Kaplan J

    更新日期:1990-12-01 00:00:00