Glutamine synthetase induction by glucocorticoids in the glucocorticoid-sensitive human leukemic cell line CEM-C7.


:Treatment of CEM-C7 cells with glucocorticoids produces a 2.5-fold increase in the activity of the enzyme glutamine synthetase (GS). This increase is specific for steroids with glucocorticoid activity adn occurs over a range of steroid concentrations consistent with a receptor-mediated mechanism. Half-maximal and maximal inductions by dexamethasone (dex) occur at 2 X 10(-8) M and 2 X 10(-7) M dex, respectively, concentrations approximately equal to those necessary to produce half and full occupancy of glucocorticoid receptors. GS activity began to increase 1 hour after dex treatment and was complete by 12 hours. This is well before any of the growth inhibitory or cytolytic effects of dex on this cell line occur. This increase was dependent on the presence of glucocorticoid receptors and required both RNA and protein synthesis. Removal of dex following stimulation to maximal levels resulted in a decrease of GS activity to preinduced levels with a half-time of 5 hours. Glutamine deprivation of cells resulted in increased GS activity. However, even in the total absence of glutamine, dex treatment elicited a 2.0-2.5-fold increase in GS activity, ruling out inhibition of glutamine uptake as a mechanism for the dex-induced increase. Experiments with 5'-bromodeoxyuridine (BrdU) demonstrated that GS elevation was sensitive to BrdU substitution of DNA, while dex-induced growth inhibition was not. Therefore GS elevation and growth inhibition in this cell line appear to be independently expressed steroid responses.


J Cell Physiol


Harmon JM,Thompson EB




Has Abstract


1982-02-01 00:00:00












  • Modulating calcium-mediated NFATc1 and mitogen-activated protein kinase deactivation underlies the inhibitory effects of kavain on osteoclastogenesis and bone resorption.

    abstract::Osteoclasts are responsible for bone resorption during the process of bone remodeling. Increased osteoclast numbers and bone resorption activity are the main factors contributing to bone loss-related diseases such as osteoporosis. Therefore, modulating the formation and function of osteoclasts is critical for the effe...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Guo Q,Cao Z,Wu B,Chen F,Tickner J,Wang Z,Qiu H,Wang C,Chen K,Tan R,Gao Q,Xu J

    更新日期:2018-01-01 00:00:00

  • TNF-alpha in vivo stimulates apoptosis in fibroblasts through caspase-8 activation and modulates the expression of pro-apoptotic genes.

    abstract::Apoptosis of matrix producing cells is common among many inflammatory diseases. The goal of the present study was to examine the apoptotic effects of tumor necrosis factor-alpha (TNF-alpha) on fibroblastic cells in vivo and to investigate the role of different caspases in this process. This was accomplished in vivo by...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Alikhani M,Alikhani Z,Raptis M,Graves DT

    更新日期:2004-12-01 00:00:00

  • Adenosine uptake, transport, and metabolism in human erythrocytes.

    abstract::Using rapid kinetic techniques, we have determined the kinetics of zero-trans influx and equilibrium exchange of adenosine, and its uptake and in situ phosphorylation at 25 degrees C in human erythrocytes which were pretreated with 2'-deoxycoformycin to inhibit deamination of adenosine. Both the Km and Vmax for adenos...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Plagemann PG,Wohlhueter RM,Kraupp M

    更新日期:1985-11-01 00:00:00

  • Retinoic acid-induced differentiation in a human neuroblastoma cell line is associated with an increase in nitric oxide synthesis.

    abstract::The human neuroblastoma cell line SK-N-BE, after incubation with 10 microM retinoic acid (RA) or 20 nM phorbol 12-myristate 13-acetate (PMA), underwent biochemical and morphological signs of differentiation within 10-14 days. In parallel, SK-N-BE cells produced significantly higher amounts of nitric oxide (NO) in comp...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ghigo D,Priotto C,Migliorino D,Geromin D,Franchino C,Todde R,Costamagna C,Pescarmona G,Bosia A

    更新日期:1998-01-01 00:00:00

  • Keeping Wnt signalosome in check by vesicular traffic.

    abstract::Wg/Wnts are paracrine and autocrine ligands that activate distinct signaling pathways while being internalized through surface receptors. Converging and contrasting views are shaping our understanding of whether, where, and how endocytosis may modulate Wnt signaling. We gather considerable amount of evidences to elabo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Feng Q,Gao N

    更新日期:2015-06-01 00:00:00

  • Myocardial proteases and cardiac remodeling.

    abstract::Cardiac hypertrophy (CH), characterized by the enlargement of cardiomyocytes, fibrosis and apoptosis, is one of the leading causes of death worldwide. Despite the advances in cardiovascular research, there remains a need to further investigate the signaling pathways that mediate CH in order to identify novel therapeut...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Riaz S,Zeidan A,Mraiche F

    更新日期:2017-12-01 00:00:00

  • Hypoxia impairs cell fusion and differentiation process in human cytotrophoblast, in vitro.

    abstract::During human pregnancy, the trophoblast develops from differentiation of cytotrophoblast cells into an endocrine active syncytiotrophoblast. In culture, isolated mononuclear cytotrophoblasts aggregate and then fuse to form a syncytium, reproducing the in vivo process. In this study, we examined the effect of low oxyge...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Alsat E,Wyplosz P,Malassiné A,Guibourdenche J,Porquet D,Nessmann C,Evain-Brion D

    更新日期:1996-08-01 00:00:00

  • Regulation of lipopolysaccharide-induced inflammatory response and endotoxemia by beta-arrestins.

    abstract::Beta-arrestins are scaffolding proteins implicated as negative regulators of TLR4 signaling in macrophages and fibroblasts. Unexpectedly, we found that beta-arrestin-1 (beta-arr-1) and -2 knockout (KO) mice are protected from TLR4-mediated endotoxic shock and lethality. To identify the potential mechanisms involved, w...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Porter KJ,Gonipeta B,Parvataneni S,Appledorn DM,Patial S,Sharma D,Gangur V,Amalfitano A,Parameswaran N

    更新日期:2010-11-01 00:00:00

  • Calcium induces differentiation of primary human salivary acinar cells.

    abstract::Cultivation of human parotid glands in serum-free medium (Ca(2+) concentration, 0.2 mM) with growth supplements resulted in isolation of a homogeneous population of epithelial cells without any mesenchymal cells. The isolated cells showed an undifferentiated phenotype with scant cytoplasmic organelles, and low levels ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hiraki A,Shirasuna K,Ikari T,Shinohara M,Garrod DR

    更新日期:2002-10-01 00:00:00

  • Stress kinase p38 mediates EGFR transactivation by hyperosmolar concentrations of sorbitol.

    abstract::Activation of the epidermal growth factor receptor (EGFR) has been shown to occur by ligand-dependent and ligand-independent mechanisms. Different molecular mechanisms have been found to be responsible for ligand-independent receptor transactivation. Here, we show that hyperosmolar concentrations of sorbitol activate ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cheng H,Kartenbeck J,Kabsch K,Mao X,Marqués M,Alonso A

    更新日期:2002-08-01 00:00:00

  • Cyanidin Chloride inhibits ovariectomy-induced osteoporosis by suppressing RANKL-mediated osteoclastogenesis and associated signaling pathways.

    abstract::Over-production and activation of osteoclasts is a common feature of osteolytic conditions such as osteoporosis, tumor-associated osteolysis, and inflammatory bone erosion. Cyanidin Chloride, a subclass of anthocyanin, displays antioxidant and anti-carcinogenesis properties, but its role in osteoclastic bone resorptio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cheng J,Zhou L,Liu Q,Tickner J,Tan Z,Li X,Liu M,Lin X,Wang T,Pavlos NJ,Zhao J,Xu J

    更新日期:2018-03-01 00:00:00

  • Granulocyte-macrophage colony formation in serum-free culture: effects of purified colony-stimulating factors and modulation by hydrocortisone.

    abstract::The effects of three purified colony-stimulating factors (CSFs) with different specificities for the granulocyte (G) and macrophage (M) lineages (G-CSF, CSF-1 and GM-CSF) were studied in a serum-free clonal assay system. The results were compared with those obtained in similar cultures containing fetal calf serum (FCS...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Eliason JF

    更新日期:1986-08-01 00:00:00

  • Increased P-glycoprotein messenger RNA stability in rat liver tumors in vivo.

    abstract::P-glycoproteins (Pgp) are comprised of a small family of plasma membrane proteins whose abundance in cultured cells is often associated with the multidrug resistance phenotype. Overexpression of Pgp has been observed in many types of human cancers, but the molecular basis for this overexpression has not been establish...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lee CH,Bradley G,Ling V

    更新日期:1998-10-01 00:00:00

  • Depleted level of heparan sulfate proteoglycan in the extracellular matrix of endothelial cell cultures exposed to endotoxin.

    abstract::Exposure of cultured endothelial cells to endotoxin causes an increase in the amount of cellular heparan sulfate proteoglycan and a depletion of this molecule in the extracellular matrix. Concomitant with the decrease in the extracellular matrix is the appearance of a fraction of proteoglycan bearing altered carbohydr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Colburn P,Kobayashi E,Buonassisi V

    更新日期:1994-04-01 00:00:00

  • Syndecans in chronic inflammatory and autoimmune diseases: Pathological insights and therapeutic opportunities.

    abstract::Syndecans (SDCs) are a family of heparan sulfate proteoglycans (HSPGs) glycoproteins ubiquitously expressed on the cell surfaces and extracellular matrix of all mammalian tissues. There are four mammalian syndecans, SDC-1 thorough 4, which play a critical role in cell adhesion, migration, proliferation, differentiatio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Agere SA,Kim EY,Akhtar N,Ahmed S

    更新日期:2018-09-01 00:00:00

  • A new apoptotic pathway for the complement factor B-derived fragment Bb.

    abstract::Apoptosis is involved in both the cellular and humoral immune system destroying tumors. An apoptosis-inducing factor from HL-60 myeloid leukemia cells was obtained, purified, and sequenced. The protein found has been identified as a human complement factor B-derived fragment Bb, although it is known that factor B is a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Uwai M,Terui Y,Mishima Y,Tomizuka H,Ikeda M,Itoh T,Mori M,Ueda M,Inoue R,Yamada M,Hayasawa H,Horiuchi T,Niho Y,Matsumoto M,Ishizaka Y,Ikeda K,Ozawa K,Hatake K

    更新日期:2000-11-01 00:00:00

  • Regulating the transcriptomes that mediate the conversion of fibroblasts to various nervous system neural cell types.

    abstract::Our understanding of the mechanism of cell fate transition during the direct reprogramming of fibroblasts into various central nervous system (CNS) neural cell types has been limited by the lack of a comprehensive analysis on generated cells, independently and in comparison with other CNS neural cell types. Here, we a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Khazaei N,Rastegar-Pouyani S,O'Toole N,Wee P,Mohammadnia A,Yaqubi M

    更新日期:2018-04-01 00:00:00

  • The pro-apoptotic effect of quercetin in cancer cell lines requires ERβ-dependent signals.

    abstract::Quercetin has potentially beneficial effects on disease prevention, including cancer. An intriguing issue regarding the mechanisms of action of quercetin is the ability of this drug to modulate estrogen receptor (ER) activities. In a previous study, we demonstrated that quercetin elicited apoptosis through an ERα-depe...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bulzomi P,Galluzzo P,Bolli A,Leone S,Acconcia F,Marino M

    更新日期:2012-05-01 00:00:00

  • Adaptive enhancement of amino acid uptake and exodus by thymic lymphocytes: influence of pH.

    abstract::Entry of certain free amino acids (alpha aminoisobutyric acid (AIB), alanine and proline), but not of leucine into rat thymic lymphocytes increased progressively when the cells were incubated in amino acid deficient medium. Actinomycin D, cycloheximide, or a high concentration of AIB abolished the time-related increas...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Peck WA,Rockwell LH,Lichtman MA

    更新日期:1976-11-01 00:00:00

  • Long noncoding RNA HOXC13-AS positively affects cell proliferation and invasion in nasopharyngeal carcinoma via modulating miR-383-3p/HMGA2 axis.

    abstract::Long noncoding RNAs have been reported to be important regulators in numerous cancers. In this study, we found that HOXC13 antisense RNA (HOXC13-AS) was highly expressed in head and neck squamous carcinoma (HNSC) tissues in The Cancer Genome Atlas database. Nasopharyngeal carcinoma (NPC) belongs to HNSC. Therefore, we...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Gao C,Lu W,Lou W,Wang L,Xu Q

    更新日期:2019-08-01 00:00:00

  • Journal of Cellular Physiology: Volume 230, Number 2, February 2015.

    abstract::Cover: Diagram illustrating the chromatic median analysis. See article by Pliss et al. on pages 427-439. ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章



    更新日期:2015-02-01 00:00:00

  • Evaluation of BAG3 levels in healthy subjects, hypertensive patients, and hypertensive diabetic patients.

    abstract::BAG3 is a member of human BAG (Bcl-2-associated athanogene) proteins and plays a role in apoptosis, cell adhesion, cytoskeleton remodeling, and autophagy. The aim of this study was to evaluate BAG3 levels in healthy subjects, hypertensive patients, and hypertensive diabetic patients. We enrolled 209 Caucasian adults, ...

    journal_title:Journal of cellular physiology

    pub_type: 临床试验,杂志文章


    authors: Derosa G,Maffioli P,Rosati A,M M,Basile A,D'Angelo A,Romano D,Sahebkar A,Falco A,Turco MC

    更新日期:2018-03-01 00:00:00

  • FGF2-mediated upregulation of urokinase-type plasminogen activator expression requires a MAP-kinase dependent activation of poly(ADP-ribose) polymerase.

    abstract::Poly(ADP-ribosyl)ation is a post-translational modification of protein occurring in the nucleus by poly(ADP-ribose) polymerase enzyme activity. The main role of poly(ADP-ribose) polymerase system as "nick sensor" and DNA breaks repair is based on its activation via DNA strand breaks. Furthermore, poly(ADP-ribose) poly...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Caldini R,Barletta E,Del Rosso M,Giovannelli L,Chevanne M

    更新日期:2005-01-01 00:00:00

  • Cytochrome P450 (CYP) 2J2 gene transfection attenuates MMP-9 via inhibition of NF-kappabeta in hyperhomocysteinemia.

    abstract::Hyperhomocysteinemia (HHcy) is associated with atherosclerotic events involving the modulation of arachidonic acid (AA) metabolism and the activation of matrix metalloproteinase-9 (MMP-9). Cytochrome P450 (CYP) epoxygenase-2J2 (CYP2J2) is abundant in the heart endothelium, and its AA metabolites epoxyeicosatrienoic ac...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Moshal KS,Zeldin DC,Sithu SD,Sen U,Tyagi N,Kumar M,Hughes WM Jr,Metreveli N,Rosenberger DS,Singh M,Vacek TP,Rodriguez WE,Ayotunde A,Tyagi SC

    更新日期:2008-06-01 00:00:00

  • Brain-derived neurotrophic factor induces migration of endothelial cells through a TrkB-ERK-integrin αVβ3-FAK cascade.

    abstract::Brain-derived neurotrophic factor (BDNF) promotes the regeneration of periodontal tissue. Since angiogenesis is important for tissue regeneration, investigating effect of BDNF on endothelial cell function may help to reveal its mechanism, whereby, BDNF promotes periodontal tissue regeneration. In this study, we examin...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Matsuda S,Fujita T,Kajiya M,Takeda K,Shiba H,Kawaguchi H,Kurihara H

    更新日期:2012-05-01 00:00:00

  • Contriving multiepitope subunit vaccine by exploiting structural and nonstructural viral proteins to prevent Epstein-Barr virus-associated malignancy.

    abstract::Cancer is one of the common lifestyle diseases and is considered to be the leading cause of death worldwide. Epstein-Barr virus (EBV)-infected individuals remain asymptomatic; but under certain stress conditions, EBV may lead to the development of cancers such as Burkitt's and Hodgkin's lymphoma and nasopharyngeal car...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ojha R,Nandani R,Prajapati VK

    更新日期:2019-05-01 00:00:00

  • Identification of lncRNA-miRNA-mRNA regulatory network associated with epithelial ovarian cancer cisplatin-resistant.

    abstract::To construct a long noncoding RNA (lncRNA)-microRNA (miRNA)-messenger RNA (mRNA) regulatory network related to epithelial ovarian cancer (EOC) cisplatin-resistant, differentially expressed genes (DEGs), differentially expressed lncRNAs (DELs), and differentially expressed miRNAs (DEMs) between MDAH and TOV-112D cells ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhao X,Tang DY,Zuo X,Zhang TD,Wang C

    更新日期:2019-11-01 00:00:00

  • Transforming growth factor-beta 1 (TGF-beta 1) up-regulation of collagen type II in primary cultures of rabbit articular chondrocytes (RAC) involves increased mRNA levels without affecting mRNA stability and procollagen processing.

    abstract::The effect of transforming growth factor-beta 1 (TGF-beta 1) on collagen biosynthesis was investigated in confluent primary monolayer cultures of rabbit articular chondrocytes (RAC). Exposure to TGF-beta (0.1, 1, and 10 ng/ml) in serum-free medium caused a dose- and time-dependent stimulation of collagen biosynthesis ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Galéra P,Vivien D,Pronost S,Bonaventure J,Rédini F,Loyau G,Pujol JP

    更新日期:1992-12-01 00:00:00

  • Production of insulin-like growth factor II (IGF-II) and different forms of IGF-binding proteins by HT-29 human colon carcinoma cell line.

    abstract::The serum-free medium conditioned by the human colon cancer cell line HT-29 contains insulin-like growth factors (IGF) that are entirely complexed to binding proteins (IGF-BP). Gel filtration in acid conditions of the cell-conditioned medium permits separation of IGF-BP from two molecular forms of IGF of 15,000 and 7,...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Culouscou JM,Remacle-Bonnet M,Garrouste F,Fantini J,Marvaldi J,Pommier G

    更新日期:1990-06-01 00:00:00

  • Novel smoothened antagonists as anti-neoplastic agents for the treatment of osteosarcoma.

    abstract::Osteosarcoma (OS) is an ultra-rare highly malignant tumor of the skeletal system affecting mainly children and young adults and it is characterized by an extremely aggressive clinical course. OS patients are currently treated with chemotherapy and complete surgical resection of cancer tissue. However, resistance to ch...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bernardini G,Geminiani M,Gambassi S,Orlandini M,Petricci E,Marzocchi B,Laschi M,Taddei M,Manetti F,Santucci A

    更新日期:2018-06-01 00:00:00