Abstract:
:Epidermal growth factor (EGF) is a small protein that acts as a mitogen for various epidermal, epithelial, and fibroblastic cells that bear specific EGF receptors. The molecule that binds EGF is a 175-kD transmembrane protein, with an extracellular ligand binding domain and an intracellular domain that possesses tyrosine kinase activity, thought to be involved in the mitogenic signalling process. Here we have constructed a recombinant murine retrovirus that transduces a human cDNA encoding the 175-kD protein and used this retrovirus to infect BAF3, a murine, bone marrow-derived cell line, which is dependent on the haematopoietic factor interleukin-3 (IL3) for its growth in culture. The EGF receptors expressed in the infected cells exhibit two affinity states, as well as EGF-stimulated autophosphorylation. Furthermore, EGF can replace IL3 in supporting short-term proliferation of these cells. These data identify functional properties of the EGF receptor upon expression of the 175-kD EGF binding protein in a haemotopoietic cell that does not express endogenous receptors. They also suggest that gene transfer of growth factor receptors to heterologous cells may allow novel growth stimuli to be exploited.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Collins MK,Downward J,Miyajima A,Maruyama K,Arai K,Mulligan RCdoi
10.1002/jcp.1041370212subject
Has Abstractpub_date
1988-11-01 00:00:00pages
293-8issue
2eissn
0021-9541issn
1097-4652journal_volume
137pub_type
杂志文章abstract::Glycochenodeoxycholate (GCDA) is closely associated with carcinogenesis and chemoresistance of hepatocellular carcinoma (HCC). Signal transducer and activator of transcription 3 (STAT3), a transcription factor, is involved in various human tumors. Whether GCDA induces chemoresistance through STAT3 and the mechanism of...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29159
更新日期:2020-03-01 00:00:00
abstract::Transient Receptor Potential Vanilloid type 1 channel (TRPV1) is an important endogenous transducer of noxious heat and chemical stimuli and is required during development of inflammatory hypersensitivity. The transcription factor Runx1 is known to play a relevant role in sensory neuron differentiation as it controls ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24236
更新日期:2013-04-01 00:00:00
abstract::Prostate cancer (PCa) is one of the most common cancers and the fifth most common reason for cancer deaths in the males. Surgical castration combined with androgen deprivation therapy, antiandrogens, and androgen synthesis inhibitors is the current therapeutic modalities for PCa. These strategies inhibit androgen synt...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.29275
更新日期:2020-04-01 00:00:00
abstract::This study examines the mechanism by which TGF-beta 1, an important mediator of cell growth and differentiation, blocks the differentiation of normal rat diploid fetal osteoblasts in vitro. We have established that the inability for pre-osteoblasts to differentiate is associated with changes in the expression of cell ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041600214
更新日期:1994-08-01 00:00:00
abstract::Adenosine is an ubiquitous nucleoside present in all body cells. It is released from metabolically active or stressed cells and subsequently acts as a regulatory molecule through binding to specific A1, A2A, A2B and A3 cell surface receptors. The synthesis of agonists and antagonists to the adenosine receptors and the...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/1097-4652(200101)186:1<19::AID-JCP1011>3.0
更新日期:2001-01-01 00:00:00
abstract::Tight junctions govern the paracellular permeability of endothelial and epithelial cells. Aberrations of tight junction function are an early and key event during the vascular spread of cancer and inflammation. This study sought to determine the role of estrogen in the regulation of tight junctions and expression of m...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10315
更新日期:2003-08-01 00:00:00
abstract::Insufficient invasion ability of trophoblasts might be associated with the development of recurrent miscarriage (RM). Ubiquitin-specific protease 25 (USP25) can regulate the processes of invasion and migration in different types of cancer cells. However, the effect of USP25 on trophoblasts and its roles in the develop...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28593
更新日期:2019-11-01 00:00:00
abstract::Progesterone is an endogenous immunomodulator and can suppress T-cell activation during pregnancy. We have previously shown that the non-genomic effects of progesterone, especially acidification, are exerted via plasma membrane sites and suppress cellular genomic responses to mitogens. This study aimed to show that ac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20962
更新日期:2007-05-01 00:00:00
abstract::Adrenoreceptors (ARs) are widely expressed and play essential roles throughout the body. Different subtype adrenoceptors elicit distinct effects on cell proliferation, but knowledge remains scarce about the subtype-specific effects of β2-ARs on the proliferation of embryonic pluripotent stem (PS) cells that represent ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24937
更新日期:2015-11-01 00:00:00
abstract::The influence upon different cellular and humoral parameters of hemopoiesis of three structurally unrelated, highly purified bacterial cell-wall components (BCWC) was investigated. The spleens of C57BL/6 mice assayed 6 days after the injection of either lipid A or outer-membrane lipoportein, but not murein, showed a m...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041050116
更新日期:1980-10-01 00:00:00
abstract::Methylxantine derivative, caffeine, is known to prevent the p53-dependent apoptosis pathway via inhibition of ATM (ataxia telangiectasia mutated) kinase, which activates p53 by phosphorylation of the Ser-15 residue. In contrast, it has been reported that caffeine induces p53-mediated apoptosis through Bax protein in n...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10289
更新日期:2003-08-01 00:00:00
abstract::Apoptosis constitutes a response of organisms to various physiological or pathological stimuli, and to different stresses. The ability of thermotolerance induced at a mild temperature of 40 degrees C to protect against activation of the apoptotic cascade by heat shock was investigated. When Chinese hamster ovary and h...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20386
更新日期:2005-10-01 00:00:00
abstract::Hybridization analysis of mRNA with a cDNA probe for human sucrase-isomaltase, pulse-chase experiments with L-[35S]-methionine followed by SDS-PAGE, and immunofluorescence detection of sucrase-isomaltase were used to analyze the level(s) at which forskolin interferes with the expression of the enzyme in Caco-2 cells i...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410322
更新日期:1989-12-01 00:00:00
abstract::Di- and tripeptides and peptide mimetics such as beta-lactam antibiotics are efficiently reabsorbed from the tubular lumen by a high-affinity peptide transporter. We have recently identified and characterized this H+-coupled high-affinity peptide transport system in the porcine proximal tubular cell line LLC-PK1. Here...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199903)178:3<341::AID-JCP8
更新日期:1999-03-01 00:00:00
abstract::Cell culture conditions can vary between laboratories and have been optimised for 2D cell culture. In this study, engineered muscle was cultured in 5.5 mM low glucose (LG) or 25 mM high glucose (HG) and in the absence or presence (+S) of streptomycin and the effect on C2C12 tissue-engineered muscle function and metabo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24857
更新日期:2015-06-01 00:00:00
abstract::The biological roles of sphingosine 1-phosphate (S1P) and S1P receptors (S1PRs) have been broadly investigated. However, at present pathophysiological roles of S1P/S1PRs axis in liver fibrosis are not well defined. Here, we investigated the functions of S1P/S1PRs axis in human hepatic stellate cells (HSC) line, LX-2 c...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22572
更新日期:2011-09-01 00:00:00
abstract::We have examined the effects of constitutive expression of PTHrP on the growth and differentiation of populations of cells derived from a clonal chondrocytic cell line, CFK2. Cells were stably transfected with cDNA encoding either full-length, secretory PTHrP (CFK2P) or nonsecretory PTHrP (CFK2P-SS). In cultures of ce...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199610)169:1<33::AID-JCP4>
更新日期:1996-10-01 00:00:00
abstract::When HL60 cells are exposed to 1,25-dihydroxyvitamin D3 (1,25D3), they undergo changes approximating the phenotype of the monocyte. Little is known, however, about the regulation and the mechanisms of this transition. It was previously noted that DNA binding by the Sp1 transcription factor in nuclear extracts of HL60 ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199805)175:2<121::AID-JCP1
更新日期:1998-05-01 00:00:00
abstract::Past work has shown that delta-opioid receptor (DOR) activation by [D-Ala(2),D-Leu(5)]-enkephalin (DADLE) attenuated the disruption of K(+) homeostasis induced by hypoxia or oxygen-glucose deprivation (OGD) in the cortex, while naltrindole, a DOR antagonist blocked this effect, suggesting that DOR activity stabilizes ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21000
更新日期:2007-07-01 00:00:00
abstract::Isolated hamster intestinal epithelial cells can be separated by velocity sedimentationion on 2-10% Ficoll gradients into three subpopulations of cells which differ in morphology, biochemistry, physiology, and membrane components. These subpopulations are not pure but are enriched in a single cell type to the extent t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041110111
更新日期:1982-04-01 00:00:00
abstract::An isoform of the Na(+)/Ca(2+) exchanger (SDNCX1.10) was cloned from mesangial cells of Sprague-Dawley rat. Regulation of this isoform was compared to two other clones that were derived from the Dahl/Rapp salt sensitive (SNCX) and salt resistant rat (RNCX). All isoforms differ at the alternative splice site and at ami...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10447
更新日期:2004-05-01 00:00:00
abstract::Incubation of alveolar macrophages or hepatocytes in media in which Na+ is replaced by K+ ("isotonic-K buffer") inhibited the movement of internalized ligand from late endosomes to lysosomes (Ward et al.: Journal of Cell Biology 110:1013-1022, 1990). In this study we investigate the mechanism responsible for the isoto...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041450319
更新日期:1990-12-01 00:00:00
abstract::The present work reports the beneficial effects of using a microplatform on the development of mouse single blastomeres (SBs) to the blastocyst stage. Development of blastocysts from SBs separated from two- and four-cell stage embryos (two- and four-cell SBs) can provide a valuable supply both for couples who use fert...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26879
更新日期:2018-12-01 00:00:00
abstract::The growth-regulated vimentin gene contains a functional double AP-1 binding site formed by two nearly perfect inverted repeats. We present evidence for down-regulation of vimentin expression by the retinoic acid receptor (RAR) in two mesodermally derived cell types. By mutation analysis we prove that the double conse...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041640111
更新日期:1995-07-01 00:00:00
abstract::Previous studies from our laboratory have shown that Chinese hamster V79 cells mutated to high level resistance to amphotericin B have a lower cellular level of cholesterol, the target molecule for the polyene antibiotic. Two amphotericin B-resistant (AMBR) mutants were each hybridized to their parental amphotericin B...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041060106
更新日期:1981-01-01 00:00:00
abstract::The carboxyl-terminal domain (CTD) of the largest subunit of mammalian RNA polymerase II (RNAP II) consists of 52 repeats of a consensus heptapeptide and is subject to phosphorylation and dephosphorylation events during each round of transcription. RNAP II activity is regulated during the cell cycle and cell cycle-dep...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.10058
更新日期:2002-02-01 00:00:00
abstract::Data on the biological effects of some overexpressed oncogenes and their cooperation with cellular factors are, at least partially, contradictory. There are reports on the strong pro-apoptotic action of temperature-sensitive (ts) p53(135val) in transformed cells at permissive temperature. However, in our experience ve...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21705
更新日期:2009-05-01 00:00:00
abstract::The basal lamina protein, laminin, has been shown to promote migration and proliferation of cultured skeletal myoblasts, resulting in increased myotube formation. However, skeletal myotubes adhere poorly to a laminin substrate, and long-term cultures of skeletal myotubes on laminin have not been achieved. We have foun...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041500205
更新日期:1992-02-01 00:00:00
abstract::Cation-Cl- cotransporters (CCCs) belong to a large family of proteins that includes 9 isoforms, two of which have still not been ascribed a transport function (CCC8 and CCC9) while the others are all known to promote Cl(-)-coupled Na+ and/or K+ movement at the cell surface. The CCCs are also included in a larger famil...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21814
更新日期:2009-09-01 00:00:00
abstract::Smooth muscle responds to IP(3)-generating agonists by producing Ca(2+) waves. Here, the mechanism of wave progression has been investigated in voltage-clamped single smooth muscle cells using localized photolysis of caged IP(3) and the caged Ca(2+) buffer diazo-2. Waves, evoked by the IP(3)-generating agonist carbach...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22103
更新日期:2010-08-01 00:00:00