Abstract：
:A sensitive serum-free culture system was used to demonstrate that cells derived from normal human skin release soluble mediators which can modulate keratinocyte, melanocyte, and fibroblast growth in vitro. In M199 supplemented with epidermal growth factor, hydrocortisone, insulin, transferrin, triidothyronine, bovine serum albumin, and an extract of bovine hypothalamus, keratinocytes underwent approximately three to five cumulative population doublings (CPD) over a 7-day period. Addition of 20% keratinocyte-conditioned medium more than doubled keratinocyte growth and increased fibroblast growth 25-40% above controls. Dermal fibroblasts maintained in the same serum-free hormone-supplemented medium (SFHSM) alone underwent approximately 4.5-5.5 CPD over a 7-day period; 20% fibroblast-conditioned medium increased fibroblast growth 50-80% and increased keratinocyte growth 30-50%. Melanocytes maintained in the same SFHSM underwent approximately 1 CPD over a 14-day period and 2 CPD if the medium was supplemented with 2% fetal bovine serum (FBS). Addition of 20% melanocyte-conditioned medium more than doubled melanocyte growth in either SFHSM or in medium containing 2% FBS, but decreased both keratinocyte and fibroblast growth up to 30-60%. None of the conditioned media altered cellular morphology. These data provide the first demonstration of mutual growth modulation by cell types normally contiguous in vivo and expand existing evidence for autocrine and paracrine growth regulation by normal human cells.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Gilchrest BA,Karassik RL,Wilkins LM,Vrabel MA,Maciag Tdoi
10.1002/jcp.1041170215subject
Has Abstractpub_date
1983-11-01 00:00:00pages
235-40issue
2eissn
0021-9541issn
1097-4652journal_volume
117pub_type
杂志文章abstract：:Electrical stimulation (ES) has been shown to improve some of impairments after spinal cord injury (SCI), but the underlying mechanisms remain unclear. The Wnt signaling pathways and the endocannabinoid system appear to be modulated in response to SCI. This study aimed to investigate the effect of ES therapy on the ac...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29793
更新日期：2020-12-01 00:00:00
abstract：:We have previously demonstrated that kidney embryonic structures are present in rats, and are still developing until postnatal Day 20. Consequently, at postnatal Day 10, the rat renal papilla contains newly formed collecting duct (CD) cells and others in a more mature stage. Performing primary cultures, combined with ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28016
更新日期：2019-08-01 00:00:00
abstract：:Considerable evidence has accumulated for a role of a nonsteroidal mevalonate product in the regulation of DNA replication and cell division. We report here a similar requirement for mevalonate in a nonreplicative function, that of natural killer (NK) cell cytotoxicity. Treatment of NK cells with 10 microM compactin f...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041390314
更新日期：1989-06-01 00:00:00
abstract：:The glycoprotein laminin appears to function in the attachment of various epithelial cells to basement membranes. We examined whether its putative cell-adhesive activity could be analyzed in a simple, one-component model system--the agglutination of erythrocytes. Laminin is a potent agglutinin of aldehyde-fixed sheep ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041140302
更新日期：1983-03-01 00:00:00
abstract：:Tumors and multicellular tumor spheroids can develop gradients in oxygen concentration, glucose concentration, and extracellular pH as they grow. In order to calculate these gradients and assess their impact on tumor growth, it is necessary to quantify the effect of these variables on tumor cell metabolism and growth....
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041510220
更新日期：1992-05-01 00:00:00
abstract：:We have previously observed major differences in the phosphorylation of membrane proteins in sparse, proliferating versus confluent, quiescent pig aortic endothelial cells (EC) (Kazlauskas and DiCorleto, 1987). In the present study we examined whether EC growth state can influence the activity of a specific phosphoryl...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041360306
更新日期：1988-09-01 00:00:00
abstract：:Fibroblast growth factor (FGF)-21 is a novel regulator of insulin-independent glucose transport in 3T3-L1 adipocytes and has glucose and triglyceride lowering effects in rodent models of diabetes. The precise mechanisms whereby FGF-21 regulates metabolism remain to be determined. Here we describe the early signaling e...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20847
更新日期：2007-01-01 00:00:00
abstract：:Cells from kidney proximal tubules have been successfully isolated, characterized, and cultured from male Fischer 344 rats between 150-400 g using a two-step collagenase perfusion. The cells undergo high levels of DNA synthesis and mitosis in both serum free media (with an without hormone supplementation) and media co...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041310116
更新日期：1987-04-01 00:00:00
abstract：:Diabetes mellitus (DM) induces bone deterioration, while mechanical stimulation promotes osteocyte-driven bone formation. We aimed to evaluate the interaction of acute exposure (24 h) to high glucose (HG) with both the pro-survival effect conferred to osteocytic MLO-Y4 cells and osteoblastic MC3T3-E1 cells by mechanic...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25829
更新日期：2017-12-01 00:00:00
abstract：:Prostaglandins prevent gastrointestinal mucosal injury and promote healing following mucosal injury by various noxious agents. Preservation or repair of microvascular function appears to be crucial in these processes. The processes involved in prostaglandin-mediated repair and preservation of endothelial function are ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199605)167:2<204::AID-JCP3
更新日期：1996-05-01 00:00:00
abstract：:With the long-range goals of elucidating the biochemical genetics and the phenotypic expression, both in vitro and in vivo, of mutator strains of mammalian cells, we have isolated and partially characterized a series of drug-resistant mutants from a subline a feeder-dependent parental mouse teratocarcinoma line (PSA-1...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041150103
更新日期：1983-04-01 00:00:00
abstract：:We have analyzed the functional and morphological effects of corticosteroid hormones in a SV40-transformed rabbit cortical-ascending-limb (CAL) cell line (RC.SV2, Vandewalle et al., 1989) having mineralocorticoid (MR) and glucocorticoid (GR) receptors (Rafestin-Oblin et al., 1993). Both aldosterone and dexamethasone (...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041560222
更新日期：1993-08-01 00:00:00
abstract：:Glial fibrillary acidic protein (GFAP) is an intermediate filament that is expressed in specifically expressed auricular chondrocytes, which are good cell sources of cartilage regenerative medicine. Although our group uses GFAP as a biomarker of matrix production in the cultured auricular chondrocytes, the biological ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25771
更新日期：2017-11-01 00:00:00
abstract：:p120-catenin (p120) is an important regulator in the function and stability of E-cadherin. However, the role of p120 in the epidermis is unclear. Previous studies have shown that globally knockout of p120 caused increased epidermal proliferation but little changes in epidermal differentiation and permeability. In the ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26535
更新日期：2018-01-01 00:00:00
abstract：:Gicerin/CD146 is a cell adhesion molecule, which belongs to the immunoglobulin (Ig) superfamily. We have reported that it has a homophilic binding activity, which participates in the neurite extension from embryonic neurons. To elucidate how gicerin is involved in the neurite extension mechanism, we employed PC12 cell...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20365
更新日期：2005-08-01 00:00:00
abstract：:Members of the transforming growth factor-β (TGF-β) family of cytokines are involved in diverse physiological processes. Although TGF-β is known to play multiple roles in the mammalian central nervous system (CNS), its role in neuronal development has not been explored. We have studied the effects of TGF-β1 on the ele...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22735
更新日期：2012-01-01 00:00:00
abstract：:Intermediate subviral particles (ISVP) derived from reovirus represent a simple model system for the switch-on of transcriptase function. In such particles the endogenous transcriptase is present in a switched-off form, one step removed from the switched-on state. Switch-on of transcriptase function is an active proce...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041120104
更新日期：1982-07-01 00:00:00
abstract：:We tested the expression of genes coding receptors of a cutaneous serotoninergic/melatoninergic system in whole human skin and in normal and pathologic cultured skin cells. Evaluation of serotonin (5HT), melatonin (MT), and melatonin-related receptors (MRR) showed expression of the isoforms 5HT2B, 5HT7, and MT1 genes ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10287
更新日期：2003-07-01 00:00:00
abstract：:Aberrant glycosylation changes on many glycoproteins are often related to cancer progression and metastasis. sp2 -Iminosugar-type castanospermine analogues, inhibitors of α-glucosidases, have been reported to exhibit antitumor activity. However, their effects on cell migration and the underlying molecular mechanism ar...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25832
更新日期：2017-12-01 00:00:00
abstract：:Calreticulin is an ER calcium-storage protein, which influences gene expression and cell adhesion. In this study, we analysed the differences in adhesive properties of calreticulin under- and overexpressing fibroblasts in relation to the calmodulin- and calcium/calmodulin-dependent kinase II (CaMK II)-dependent signal...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21122
更新日期：2007-10-01 00:00:00
abstract：:For malignant cells cultured from a human astrocytoma, electrophysiological characteristics of the plasma membrane included specific resistivity of 446.82 +/- 279.5 ohm . cm2, specific capacitance of 0.758 +/- 0.52 microfarads/cm2, time constant 0.318 +/- 0.10 msec. The resting membrane potential averaged--14.07 +/- 7...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041050321
更新日期：1980-12-01 00:00:00
abstract：:When HL60 cells are exposed to 1,25-dihydroxyvitamin D3 (1,25D3), they undergo changes approximating the phenotype of the monocyte. Little is known, however, about the regulation and the mechanisms of this transition. It was previously noted that DNA binding by the Sp1 transcription factor in nuclear extracts of HL60 ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199805)175:2<121::AID-JCP1
更新日期：1998-05-01 00:00:00
abstract：:Nucleotide receptors signaling affects cell proliferation, with possible implications on tumorigenic processes. However, molecular targets and action mechanisms of the extracellular nucleotides are still poorly elucidated. We have previously shown in ARO cells that APE1/Ref-1, a transcriptional coactivator responsible...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20704
更新日期：2006-10-01 00:00:00
abstract：:Kinetic studies of binding and internalization of 125I-platelet-derived growth factor (PDGF) demonstrate that up to 15% of membrane-associated radioactivity is internalized within 2 minutes after warming to 37 degrees C in a variety of cell types. The T 1/2 for internalization is approximately 20 minutes. The T 1/2 fo...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041210202
更新日期：1984-11-01 00:00:00
abstract：:We investigated the radiosensitizing effects of Co-NMS, a derivative of nimesulide based on a cobalt carbonyl complex, on malignant glioma cells. In the zebrafish exposed to Co-NMS ranging from 5 to 20 μM, cell death and heat shock protein 70 expression in the brain and neurobehavioral performance were evaluated. Our ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29774
更新日期：2020-12-01 00:00:00
abstract：:Despite being densely packed with chromatin, nuclear bodies and a nucleoskeletal network, the nucleus is a remarkably dynamic organelle. Chromatin loops form and relax, RNA transcripts and transcription factors move diffusively, and nuclear bodies move. We show here that RNA splicing speckled domains (splicing speckle...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25224
更新日期：2016-06-01 00:00:00
abstract：:Estrogen has been shown to protect osteoblastic cells from apoptosis. Similarly, estrogen treatment preceding heat shock elevates heat shock protein 27 (hsp27) expression and increases thermoresistance in the murine estrogen receptor-transformed SMER14 osteoblastic cell line. Forced expression of hsp27 expression in o...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/1097-4652(200012)185:3<401::AID-JCP10>3.0.
更新日期：2000-12-01 00:00:00
abstract：:Cell division in fertilized sea urchin eggs was reversibly inhibited when the ketoaldehyde phenyl glyoxal (PG) at a concentration of 0.1 mM was added to eggs for ten minutes prior to the formation of the mitotic spindle. We investigated whether inhibition of mitosis was due to PG binding to the cell surface (as previo...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041000119
更新日期：1979-07-01 00:00:00
abstract：:Clear cell renal cell carcinoma (ccRCC) is a common urinary neoplasm, looking for useful candidates to establish scientific foundation for the therapy of ccRCC is urgent. We downloaded genomic profiles of GSE781, GSE6244, GSE53757, and GSE66271 from the Gene Expression Omnibus (GEO) database. GEO2R was used to analyze...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28597
更新日期：2019-11-01 00:00:00
abstract：:Basic fibroblast growth factor (bFGF, FGF-2) is progressively lost from mammary epithelial cells as they become malignant. To investigate the effects of restoring the expression of bFGF in breast cancer cells, we constructed MCF-7 cells that permanently overexpress 18-kD cytoplasm-localizing bFGF (MCF-7/deltaA(FGF)(18...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199812)177:3<411::AID-JCP5
更新日期：1998-12-01 00:00:00
