Abstract:
:We have previously demonstrated that kidney embryonic structures are present in rats, and are still developing until postnatal Day 20. Consequently, at postnatal Day 10, the rat renal papilla contains newly formed collecting duct (CD) cells and others in a more mature stage. Performing primary cultures, combined with immunocytochemical and time-lapse analysis, we investigate the cellular mechanisms that mediate the postnatal CD formation. CD cells acquired a greater degree of differentiation, as we observed that they gradually lose the ability to bind BSL-I lectin, and acquire the capacity to bind Dolichos biflorus. Because CD cells retain the same behavior in culture than in vivo, and by using DBA and BSL-I as markers of cellular lineage besides specific markers of epithelial/mesenchymal phenotype, the experimental results strongly suggest the existence of mesenchymal cell insertion into the epithelial CD sheet. We propose such a mechanism as an alternative strategy for CD growing and development.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Del Valle Guaytima E,Brandán YR,Favale NO,Sterin-Speziale NB,Márquez MGdoi
10.1002/jcp.28016subject
Has Abstractpub_date
2019-08-01 00:00:00pages
13387-13402issue
8eissn
0021-9541issn
1097-4652journal_volume
234pub_type
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