Abstract：
:Despite being densely packed with chromatin, nuclear bodies and a nucleoskeletal network, the nucleus is a remarkably dynamic organelle. Chromatin loops form and relax, RNA transcripts and transcription factors move diffusively, and nuclear bodies move. We show here that RNA splicing speckled domains (splicing speckles) fluctuate in constrained nuclear volumes and remodel their shapes. Small speckles move in a directed way toward larger speckles with which they fuse. This directed movement is reduced upon decreasing cellular ATP levels or inhibiting RNA polymerase II activity. The random movement of speckles is reduced upon decreasing cellular ATP levels, moderately reduced after inhibition of SWI/SNF chromatin remodeling and modestly increased upon inhibiting RNA polymerase II activity. To define the paths through which speckles can translocate in the nucleus, we generated a pressure gradient to create flows in the nucleus. In response to the pressure gradient, speckles moved along curvilinear paths in the nucleus. Collectively, our results demonstrate a new type of ATP-dependent motion in the nucleus. We present a model where recycling splicing factors return as part of small sub-speckles from distal sites of RNA processing to larger splicing speckles by a directed ATP-driven mechanism through interchromatin spaces.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Zhang Q,Kota KP,Alam SG,Nickerson JA,Dickinson RB,Lele TPdoi
10.1002/jcp.25224subject
Has Abstractpub_date
2016-06-01 00:00:00pages
1269-75issue
6eissn
0021-9541issn
1097-4652journal_volume
231pub_type
杂志文章abstract：:Formation of human T lymphocyte colonies in semisolid medium from T lymphocyte colony-forming units (TL-CFUs) under stimulation of phytohemagglutinin (PHA) has been reported by several authors. These TL-CFUs were present in unsensitized lymphocyte populations. We report here that such TL-CFUs are capable of renewing t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：1983-10-01 00:00:00
abstract：:Colon cancer remains one of the lethal malignancies in the world. Aberrant activation of canonical Wnt/β-catenin signaling pathway has been observed in colon cancer. In contrast, the non-canonical Wnt signaling functions remain obscure. Wnt5a is a representative non-canonical Wnt ligand which has gained extensive atte...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.24566
更新日期：2014-12-01 00:00:00
abstract：:Tumor suppressor pRb2/p130 gene belongs to the retinoblastoma (Rb) gene family, which also includes pRb/p105 and pRb/p107. The members of the Rb gene family have attracted a great deal of interest because of their essential role in regulating cell cycle and, consequently, cell proliferation. This mini review discusses...
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pub_type： 杂志文章,评审
doi：10.1002/jcp.21225
更新日期：2007-11-01 00:00:00
abstract：:Bafilomycin A1, a specific inhibitor of H(+)-ATPases of the vacuolar type, was in the present study shown, at similar concentrations, to induce secretion of lysosomal enzyme and to elevate lysosomal pH in mouse macrophages. These results lend support to the previous suggestion of a triggering role for an increase in l...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041630116
更新日期：1995-04-01 00:00:00
abstract：:Peptidoglycan (PGN) is a potent immune adjuvant derived from bacterial cell walls. Previous investigations suggest that intestinal epithelium may absorb PGN from the lumen. Nonetheless, how PGN is taken up and crosses intestinal epithelium remains largely unclear. Here, we first characterized PGN transport in vitro us...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21985
更新日期：2010-03-01 00:00:00
abstract：:In the present study, we have sought to determine whether a given signal transduction pathway can have diverse effects on subpopulations of cells of a lineage depending upon the stage of differentiation. To test this hypothesis, we selected the cyclic adenosine monophosphate (cAMP) signal transduction pathway because ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041560305
更新日期：1993-09-01 00:00:00
abstract：:Concanavalin A (ConA)-induced hepatitis is an experimental model of human autoimmune hepatitis induced in rodents by i.v. injection of Con A. The disease is characterized by increase in serum levels of transaminases and massive immune infiltration of the livers. Type 1, type 2, and type 17 cytokines play a pathogenic ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：2018-05-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22735
更新日期：2012-01-01 00:00:00
abstract：:The methylation of DNA is an epigenetic modification that can play an important role in the control of gene expression in mammalian cells. The enzyme involved in this process is DNA methyltransferase, which catalyzes the transfer of a methyl group from S-adenosyl-methionine to cytosine residues to form 5-methylcytosin...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/(SICI)1097-4652(200005)183:2<145::AID-JCP1
更新日期：2000-05-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：2015-03-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041210314
更新日期：1984-12-01 00:00:00
abstract：:Osteoporosis can result from intestinal inflammation, as is seen with inflammatory bowel disease. Probiotics, microorganisms that provide a health benefit to the host when ingested in adequate amounts, can have anti-inflammatory properties and are currently being examined to treat inflammatory bowel disease. Here, we ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24340
更新日期：2013-08-01 00:00:00
abstract：:Biosensors are composed of (bio)receptors, transducers, and detection systems and are able to convert the biological stimulus into a measurable signal. This systematic review evaluates the current state of the art of innovation and research in this field, identifying the biosensors that in vitro monitor the musculoske...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28753
更新日期：2019-12-01 00:00:00
abstract：:The present study was designed to investigate the mechanism of myeloid differentiation protein 2 (MD2) on intestinal mucosa destruction in mice with chronic colitis. Briefly, a chronic colitis mouse model was established by the administration of dextran sulfate sodium (DSS) in transgenic mice of MD2 overexpression (Tr...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28711
更新日期：2019-11-01 00:00:00
abstract：:HCF-1 functions as a coactivator for herpes simplex virus VP16 and a number of mammalian transcription factors. Mature HCF-1 is composed of two subunits generated by proteolytic cleavage of a larger precursor at six centrally-located HCF(PRO) repeats. The resulting N- and C-terminal subunits remain tightly associated ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10193
更新日期：2003-02-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25882
更新日期：2018-01-01 00:00:00
abstract：:Conditions have been established under which the antizyme of ornithine decarboxylase (E.C. 4.1.1.17, L-ornithine carboxy-lyase, ODC) a non-competitive protein inhibitor of ODC, can be detected in cells in response to as little as 10(-7) M putrescine. The maintenance of intracellular antizyme activity depends upon the ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041070206
更新日期：1981-05-01 00:00:00
abstract：:The zafirlukast has been reported to be anti-inflammatory and widely used to alleviate the symptoms of asthma. However, its influence on insulin secretion in pancreatic β-cells has not been investigated. Herein, we examined the effects of zafirlukast on insulin secretion and the potential underlying mechanisms. Among ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26750
更新日期：2018-11-01 00:00:00
abstract：:Epidermal growth factor (EGF) is a small protein that acts as a mitogen for various epidermal, epithelial, and fibroblastic cells that bear specific EGF receptors. The molecule that binds EGF is a 175-kD transmembrane protein, with an extracellular ligand binding domain and an intracellular domain that possesses tyros...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041370212
更新日期：1988-11-01 00:00:00
abstract：:We recently reported that the growth of normal rat pleural mesothelial cells (RPMCs) is inhibited by conditioned media from either in vivo or in vitro transformed RPMCs. In this study we report that the growth of normal RPMCs is inhibited by epidermal growth factor (EGF). This was demonstrated by using three methods o...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041440118
更新日期：1990-07-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/1097-4652(200008)184:2<246::AID-JCP13>3.0.
更新日期：2000-08-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
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更新日期：2013-04-01 00:00:00
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journal_title：Journal of cellular physiology
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doi：10.1002/(SICI)1097-4652(199807)176:1<10::AID-JCP2>
更新日期：1998-07-01 00:00:00
abstract：:An artificial wound in a confluent monolayer of human keratinocyte HaCaT cells or mouse embryo fibroblast Swiss NIH 3T3 cells was used to analyze the effects of the nitric oxide (NO) chemical donor, S-nitroso-N-acetylpenicillamine (SNAP). SNAP exposure promoted an enhanced rate of wound closure and accelerated motilit...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：2016-10-01 00:00:00
abstract：:Polycystin-1 regulates a number of cellular processes through the formation of complexes with the polycystin-2 ion channel or with other signal transduction proteins. Polycystin-1 is expressed in many tissues but other members of this gene family are distributed in a more restricted fashion. PKDREJ expression has been...
journal_title：Journal of cellular physiology
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更新日期：2006-11-01 00:00:00
abstract：:Adipogenesis plays a key role in the pathogenesis of obesity. It begins with the commitment of mesenchymal stem cells (MSCs) to the adipocyte lineage, followed by terminal differentiation of preadipocytes to mature adipocytes. A critical, but poorly understood, component of adipogenesis involves proliferation of MSCs ...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.22064
更新日期：2010-06-01 00:00:00
abstract：:Mesenchymal stem cells (MSC) represent emerging cell-based therapies for diabetes and associated complications. Ongoing clinical trials are using exogenous MSC to treat type 1 and 2 diabetes, cardiovascular disease and non-healing wounds due to diabetes. The majority of these trials are aimed at exploiting the ability...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24013
更新日期：2012-09-01 00:00:00
abstract：:The herbicide 2,4,5 trichlorophenoxy acetic acid (245T) at concentrations from 0.5 to 0.9 mM, was found to inhibit respiration and then growth in exponentially growing cultures of Tetrahymena pyriformis. Cell division was stopped for periods up to 60 minutes after which the cells recovered and division resumed. Recove...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040850219
更新日期：1975-04-01 00:00:00
abstract：:Aberrant promoter hypermethylation of several known or putative tumor suppressor genes occurs frequently during the etiopathogenesis of lung cancer and is a promising tool for cancer detection. In the present study, promoter hypermethylation of p16INK4A and CDH13 genes was investigated in tumor tissue and in matched s...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20503
更新日期：2006-03-01 00:00:00
abstract：:Human promyelocytic leukemia (HL-60) cells display a novel voltage-dependent outward current under voltage clamp. This current is present at low levels in the proliferative state and in granulocytes derived from HL-60 cells which were induced to differentiate with retinoic acid. It is elevated in macrophages derived f...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041320227
更新日期：1987-08-01 00:00:00
