Coordinated Dynamics of RNA Splicing Speckles in the Nucleus.


:Despite being densely packed with chromatin, nuclear bodies and a nucleoskeletal network, the nucleus is a remarkably dynamic organelle. Chromatin loops form and relax, RNA transcripts and transcription factors move diffusively, and nuclear bodies move. We show here that RNA splicing speckled domains (splicing speckles) fluctuate in constrained nuclear volumes and remodel their shapes. Small speckles move in a directed way toward larger speckles with which they fuse. This directed movement is reduced upon decreasing cellular ATP levels or inhibiting RNA polymerase II activity. The random movement of speckles is reduced upon decreasing cellular ATP levels, moderately reduced after inhibition of SWI/SNF chromatin remodeling and modestly increased upon inhibiting RNA polymerase II activity. To define the paths through which speckles can translocate in the nucleus, we generated a pressure gradient to create flows in the nucleus. In response to the pressure gradient, speckles moved along curvilinear paths in the nucleus. Collectively, our results demonstrate a new type of ATP-dependent motion in the nucleus. We present a model where recycling splicing factors return as part of small sub-speckles from distal sites of RNA processing to larger splicing speckles by a directed ATP-driven mechanism through interchromatin spaces.


J Cell Physiol


Zhang Q,Kota KP,Alam SG,Nickerson JA,Dickinson RB,Lele TP




Has Abstract


2016-06-01 00:00:00












  • Neurotransmitters and Neuropeptides: New Players in the Control of Islet of Langerhans' Cell Mass and Function.

    abstract::Islets of Langerhans control whole body glucose homeostasis, as they respond, releasing hormones, to changes in nutrient concentrations in the blood stream. The regulation of hormone secretion has been the focus of attention for a long time because it is related to many metabolic disorders, including diabetes mellitus...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Di Cairano ES,Moretti S,Marciani P,Sacchi VF,Castagna M,Davalli A,Folli F,Perego C

    更新日期:2016-04-01 00:00:00

  • Prognostic and diagnostic significance of circRNAs expression in lung cancer.

    abstract::Circular RNAs (circRNAs) have spurred considerable interest in numerous tumors. We aimed to investigate the clinical, prognostic, and diagnostic roles of circRNAs in human lung cancer. We systematically searched PubMed, Web of Science, EMBASE, Scopus, CBM, and the Cochrane Library databases up to July 24, 2018. Eligib...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Huang X,Zhang W,Shao Z

    更新日期:2019-08-01 00:00:00

  • Glucose utilization, pH reduction and density dependent inhibition in cultures of chick embryo fibroblasts.

    abstract::The multiplication rate of sparse cultures of chick embryo cells is only slightly lower at pH 6.9 than at pH 7.4. There is, however, a marked reduction in the multiplication rate of the pH 6.9 cultures before they reach confluency. Cultures at pH 7.4 continue to multiply beyond confluency with only a slight decrease i...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Fodge DW,Rubin H

    更新日期:1975-06-01 00:00:00

  • Effect of donor age and prior sun exposure on growth inhibition of cultured human dermal fibroblasts by all trans-retinoic acid.

    abstract::The effect of retinoic acid on human fibroblasts was studied in a cell culture model of chronologic aging and photoaging. During early exponential phase, all trans-retinoic acid significantly stimulated growth rate of adult arm-derived dermal fibroblasts but not of newborn or adult foreskin-derived fibroblasts. Retino...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Stanulis-Praeger BM,Gilchrest BA

    更新日期:1989-04-01 00:00:00

  • Characterization of Krt19 CreERT allele for targeting the nucleus pulposus cells in the postnatal mouse intervertebral disc.

    abstract::Intervertebral disc degeneration and associated back pain are relatively common but sparsely understood conditions, affecting over 70% of the population during some point of life. Disc degeneration is often associated with a loss of nucleus pulposus (NP) cells. Genetic mouse models offer convenient avenues to understa...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mohanty S,Pinelli R,Dahia CL

    更新日期:2020-01-01 00:00:00

  • The rate of fluid shear stress is a potent regulator for the differentiation of mesenchymal stem cells.

    abstract::We have previously demonstrated that the rate of fluid shear stress (ΔSS) can manipulate the fate of mesenchymal stem cells (MSCs) to osteogenic or chondrogenic cells. However, whether ΔSS is comparable to other two means of induction medium and substrate stiffness that have been proven to be potent in differentiation...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yue D,Zhang M,Lu J,Zhou J,Bai Y,Pan J

    更新日期:2019-02-19 00:00:00

  • Suppression of OSCC malignancy by oral glands derived-PIP identified by iTRAQ combined with 2D LC-MS/MS.

    abstract::Oral squamous cell carcinoma (OSCC) is the most common malignancy in head and neck cancer and a global cause of cancer-related death. Due to the poor survival rates associated with OSCC, there is a growing need to develop novel technologies and predictive biomarkers to improve disease diagnosis. The identification of ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wang Q,Zhi Y,Ren W,Li S,Dou Z,Xing X,Quan X,Wang Y,Jiang C,Liang X,Gao L,Zhi K

    更新日期:2019-01-28 00:00:00

  • Novel hedgehog agonists promote osteoblast differentiation in mesenchymal stem cells.

    abstract::Hedgehog (Hh) family members are involved in multiple cellular processes including proliferation, migration, differentiation, and cell fate determination. Recently, the novel Hh agonists Hh-Ag 1.3 and 1.7 were identified in a high-throughput screening of small molecule compounds that activate the expression of Gli1, a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Nakamura T,Naruse M,Chiba Y,Komori T,Sasaki K,Iwamoto M,Fukumoto S

    更新日期:2015-04-01 00:00:00

  • Sarcolectin and interferon in the regulation of cell growth.

    abstract::Sarcolectin is an endolectin present in a great variety of conjunctival tissues (muscles, cartilage, sarcomas), but also in brain or placental extracts of vertebrates, including primates. When purified to electrophoretical homogeneity as a 65-kd protein, it agglutinates cells and has an affinity for simple sugars. In ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chany-Fournier F,Jiang PH,Chany C

    更新日期:1990-10-01 00:00:00

  • c-Src kinase contributes on endothelial cells mechanotransduction in a heat shock protein 70-dependent turnover manner.

    abstract::Shear stress changes are associated with a repertory of signaling cascade modulating vascular phenotype. As shear stress-related tensional forces might be associated with pathophysiological susceptibility, a more comprehensive molecular map needs to be addressed. Thus, we subjected human umbilical vein endothelial cel...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Pinto TS,Fernandes CJDC,da Silva RA,Gomes AM,Vieira JCS,Padilha PM,Zambuzzi WF

    更新日期:2019-07-01 00:00:00

  • Neurovascular inflammation in the pathogenesis of brain arteriovenous malformations.

    abstract::Brain arteriovenous malformations (bAVM) arise as congenital or sporadic focal lesions with a significant risk for intracerebral hemorrhage (ICH). A wide range of interindividual differences is present in the onset, progression, and severity of bAVM. A growing body of gene expression and polymorphism-based research st...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Krithika S,Sumi S

    更新日期:2020-12-20 00:00:00

  • Characterization of a simian virus 40-transformed human podocyte cell line producing type IV collagen and exhibiting polarized response to atrial natriuretic peptide.

    abstract::Biology of glomerular visceral epithelial cells ("podocytes") and their role in inflammatory process remain obscure, partly because of the lack of well-differentiated podocyte cultures. We have established a human cell line by transfecting with a replication-defective SV40 plasmid (pSVHB1), a primary culture of podocy...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ardaillou N,Lelongt B,Turner N,Piedagnel R,Baudouin B,Estrade S,Cassingena R,Ronco PM

    更新日期:1992-09-01 00:00:00

  • Adipose-derived stem cells for wound healing.

    abstract::Wound healing is a complex but a fine-tuned biological process in which human skin has the ability to regenerate itself following damage. However, in particular conditions such as deep burn or diabetes the process of wound healing is compromised. Despite investigations on the potency of a wide variety of stem cells fo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Hassanshahi A,Hassanshahi M,Khabbazi S,Hosseini-Khah Z,Peymanfar Y,Ghalamkari S,Su YW,Xian CJ

    更新日期:2019-06-01 00:00:00

  • Essential Role of CFTR in PKA-Dependent Phosphorylation, Alkalinization, and Hyperpolarization During Human Sperm Capacitation.

    abstract::Mammalian sperm require to spend a limited period of time in the female reproductive tract to become competent to fertilize in a process called capacitation. It is well established that HCO3- is essential for capacitation because it activates the atypical soluble adenylate cyclase ADCY10 leading to cAMP production, an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Puga Molina LC,Pinto NA,Torres Rodríguez P,Romarowski A,Vicens Sanchez A,Visconti PE,Darszon A,Treviño CL,Buffone MG

    更新日期:2017-06-01 00:00:00

  • The calcineurin-nuclear factor of activated T cells signaling pathway mediates the effect of corticotropin releasing factor and urocortins on catecholamine synthesis.

    abstract::The biological effects of the Corticotropin-releasing factor (CRF) family of neuropeptides are mediated by mobilization of [Ca(2+)]. Aim of the current work was to examine if the calcineurin/NFAT (nuclear factor of activated T-cells) signaling pathway is involved in the effect of CRF peptides in catecholamine synthesi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dermitzaki E,Tsatsanis C,Gravanis A,Margioris AN

    更新日期:2012-05-01 00:00:00

  • Long noncoding RNA MT1JP inhibits proliferation, invasion, and migration while promoting apoptosis of glioma cells through the activation of PTEN/Akt signaling pathway.

    abstract::This study is carried out to elucidate the role of long noncoding RNAs (lncRNAs) MT1JP in proliferation, invasion, migration, and apoptosis of glioma cells through the regulation of PTEN/Akt signaling pathway. The expression of MT1JP in 80 normal brain tissues and 138 glioma tissues, as well as glioma cell lines, was ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang Y,Sui R,Chen Y,Liang H,Shi J,Piao H

    更新日期:2019-11-01 00:00:00

  • Effects of parathyroid hormone and cyclic AMP analogues on the activity of ornithine decarboxylase and expression of the differentiated phenotype of chondrocytes in culture.

    abstract::Parathyroid hormone (PTH) greatly increased the level of adenosine 3', 5' cyclic monophosphate (cAMP) in rabbit costal chondrocytes in culture 2 minutes after its addition. PTH, as well as N6 O2' dibutyryl adenosine 3', 5' cyclic monophosphate (DBcAMP) and 8 Bromo adenosine 3', 5' cyclic monophosphate (8 Br-cAMP) indu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Takigawa M,Takano T,Suzuki F

    更新日期:1981-02-01 00:00:00

  • Mechanisms regulating the expression, self-maintenance, and signaling-function of the bradykinin B2 and B1 receptors.

    abstract::Bradykinin (BK) is a potent short-lived effector belonging to a class of peptides known as kinins. It participates in inflammatory and vascular regulation and processes including angioedema, tissue permeability, vascular dilation, and smooth muscle contraction. BK exerts its biological effects through the activation o...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Prado GN,Taylor L,Zhou X,Ricupero D,Mierke DF,Polgar P

    更新日期:2002-12-01 00:00:00

  • Beta adrenergic receptor repopulation of C6 glioma cells after irreversible blockade and down regulation.

    abstract::C6 glioma cells possess beta adrenergic receptors coupled with adenylate cyclase which can be irreversibly blocked by bromoacetylaminomethylpindolol (Br-AAM-pindolol), a beta adrenergic antagonist. With 1 microM Br-AAM-pindolol, more than 80% of beta adrenergic receptors, labeled by (3H)-dihydroalprenolol [3H)-DHA), w...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Homburger V,Pantaloni C,Lucas M,Gozlan H,Bockaert J

    更新日期:1984-12-01 00:00:00

  • Contractile heterogeneity in ventricular myocardium.

    abstract::The transmural heterogeneity of the contractility in ventricular muscle has not been well-studied. Here, we investigated the calcium transient and sarcomere contraction/relaxation in the endocardial (Endo) and epicardial (Epi) myocytes. Endo and Epi myocytes were isolated from C57/BL6 mice by Langendorff perfusion. Ca...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Pan W,Yang Z,Cheng J,Qian C,Wang Y

    更新日期:2018-08-01 00:00:00

  • Exogenous glutathione improves intracellular glutathione synthesis via the γ-glutamyl cycle in bovine zygotes and cleavage embryos.

    abstract::Excess reactive oxygen species (ROS) generated in embryos during in vitro culture damage cellular macromolecules and embryo development. Glutathione (GSH) scavenges ROS and optimizes the culture system. However, how exogenous GSH influences intracellular GSH and improves the embryo developmental rate is poorly underst...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Li F,Cui L,Yu D,Hao H,Liu Y,Zhao X,Pang Y,Zhu H,Du W

    更新日期:2019-05-01 00:00:00

  • Evaluation of efficacy on RANKL induced osteoclast from RAW264.7 cells.

    abstract::Established RAW264.7 cell lines for osteoclastic differentiation has been widely engaged in bone homeostasis research, however, the efficacy of RANKL independently stimulating has rarely been defined, because protocols were usually developed and modified by various laboratories. Otherwise, problematic issues are also ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Song C,Yang X,Lei Y,Zhang Z,Smith W,Yan J,Kong L

    更新日期:2019-07-01 00:00:00

  • Effects of reduced cell volume and water content on glycolysis in L-929 cells.

    abstract::Mouse L-929 cells were subjected to increasing concentrations of sorbitol, which remove cell water and reduce volume osmotically. The rate of lactate production from glucose was significantly higher in osmotically perturbed cells than in controls, both in monolayers and in suspensions. L cells can apparently use sorbi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Clegg JS,Jackson SA,Fendl K

    更新日期:1990-02-01 00:00:00

  • The pro-apoptotic effect of quercetin in cancer cell lines requires ERβ-dependent signals.

    abstract::Quercetin has potentially beneficial effects on disease prevention, including cancer. An intriguing issue regarding the mechanisms of action of quercetin is the ability of this drug to modulate estrogen receptor (ER) activities. In a previous study, we demonstrated that quercetin elicited apoptosis through an ERα-depe...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bulzomi P,Galluzzo P,Bolli A,Leone S,Acconcia F,Marino M

    更新日期:2012-05-01 00:00:00

  • Extinction of the human insulin gene expression in insulinoma x fibroblast somatic cell hybrids involves cis-acting DNA elements.

    abstract::Insulin gene expression in rat insulinoma (RIN) cells is extinct in RIN x fibroblast hybrids and can reappear upon loss of DNA contributed by the fibroblast parent. (Besnard et al., Exp. Cell Res. 185:101-108, 1989). In the present study, we looked for the role of 5'-flanking sequences of the human insulin gene in the...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Besnard C,Monthioux E,Loràs P,Jami J,Daegelen D

    更新日期:1991-03-01 00:00:00

  • Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer.

    abstract::We reported that Disabled-2 (Dab2) is located at the apical membrane in suckling rat intestine. Here, we discovered that, in colon of suckling and adult mouse and of adult human, Dab2 is only at lateral crypt cell membrane and colocalized with E-cadherin. Dab2 depletion in Caco-2 cells led to E-cadherin internalizatio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Vazquez-Carretero MD,García-Miranda P,Balda MS,Matter K,Ilundáin AA,Peral MJ

    更新日期:2021-02-01 00:00:00

  • A novel chemically defined serum- and feeder-free medium for undifferentiated growth of porcine pluripotent stem cells.

    abstract::Development and improvement of in vitro culture system supporting self-renewal and unlimited proliferation of porcine pluripotent stem cells (pPSCs) is an indispensable process for the naïve pPSCs establishment. In this study, we modified the previous culture system and attempted to develop a novel chemically defined ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang X,Xue B,Li Y,Wei R,Yu Z,Jin J,Zhang Y,Liu Z

    更新日期:2019-01-30 00:00:00

  • Electrophysiological characterization of voltage-gated Na+ current expressed in the highly metastatic Mat-LyLu cell line of rat prostate cancer.

    abstract::Voltage-gated Na+ channels, classically associated with impulse conduction in excitable tissues, are also found in a variety of epithelial cell types where their possible functions are not known so well. We have previously reported expression of a voltage-gated Na+ channel specifically in the highly metastatic Mat-LyL...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Grimes JA,Djamgoz MB

    更新日期:1998-04-01 00:00:00

  • Intracellular chloride regulates cell proliferation through the activation of stress-activated protein kinases in MKN28 human gastric cancer cells.

    abstract::Recently, we reported that reduction of intracellular Cl(-) concentration ([Cl(-)](i)) inhibited proliferation of MKN28 gastric cancer cells by diminishing the transition rate from G(1) to S cell-cycle phase through upregulation of p21, cyclin-dependent kinase inhibitor, in a p53-independent manner. However, it is sti...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ohsawa R,Miyazaki H,Niisato N,Shiozaki A,Iwasaki Y,Otsuji E,Marunaka Y

    更新日期:2010-06-01 00:00:00

  • Stress and matrix-responsive cytoskeletal remodeling in fibroblasts.

    abstract::In areolar "loose" connective tissue, fibroblasts remodel their cytoskeleton within minutes in response to static stretch resulting in increased cell body cross-sectional area that relaxes the tissue to a lower state of resting tension. It remains unknown whether the loosely arranged collagen matrix, characteristic of...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Abbott RD,Koptiuch C,Iatridis JC,Howe AK,Badger GJ,Langevin HM

    更新日期:2013-01-01 00:00:00