Requirement for PLCγ2 in IL-3 and GM-CSF-stimulated MEK/ERK phosphorylation in murine and human hematopoietic stem/progenitor cells.

Abstract:

:Even though the involvement of intracellular Ca(2+) Ca(i)(2+) in hematopoiesis has been previously demonstrated, the relationship between Ca(i)(2+) signaling and cytokine-induced intracellular pathways remains poorly understood. Herein, the molecular mechanisms integrating Ca(2+) signaling with the extracellular signal-regulated kinase 1/2 (ERK1/2) pathway in primary murine and human hematopoietic stem/progenitor cells stimulated by IL-3 and GM-CSF were studied. Our results demonstrated that IL-3 and GM-CSF stimulation induced increased inositol 1,4,5-trisphosphate (IP(3) ) levels and Ca(i)(2+) release in murine and human hematopoietic stem/progenitor cells. In addition, Ca(i)(2+) signaling inhibitors, such as inositol 1,4,5-trisphosphate receptor antagonist (2-APB), PKC inhibitor (GF109203), and CaMKII inhibitor (KN-62), blocked phosphorylation of MEK activated by IL-3 and GM-CSF, suggesting the participation of Ca(2+) -dependent kinases in MEK activation. In addition, we identify phospholipase Cγ2 (PLCγ2) as a PLCγ responsible for the induction of Ca(2+) release by IL-3 and GM-CSF in hematopoietic stem/progenitor cells. Furthermore, the PLCγ inhibitor U73122 significantly reduced the numbers of granulocyte-macrophage colony-forming units after cytokine stimulation. Similar results were obtained in both murine and human hematopoietic stem/progenitor cells. Taken together, these data indicate a role for PLCγ2 and Ca(2+) signaling through the modulation of MEK in both murine and human hematopoietic stem/progenitor cells.

journal_name

J Cell Physiol

authors

Leon CM,Barbosa CM,Justo GZ,Borelli P,Resende JD Jr,de Oliveira JS,Ferreira AT,Paredes-Gamero EJ

doi

10.1002/jcp.22507

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

1780-92

issue

7

eissn

0021-9541

issn

1097-4652

journal_volume

226

pub_type

杂志文章
  • Growth of v-src-transformed cells in serum-free medium through the induction of growth factors.

    abstract::The v-src oncogene is one of only two oncogenes capable of transforming mouse embryo fibroblasts (MEFs) lacking the IGF-IR gene (R-cells). R-/v-src cells grow robustly in the absence of serum, suggesting the hypothesis that they may produce one or more growth factors that would sustain their ability to proliferate in ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24303

    authors: Deangelis T,Quong A,Morrione A,Baserga R

    更新日期:2013-07-01 00:00:00

  • M-CSF priming of osteoclast precursors can cause osteoclastogenesis-insensitivity, which can be prevented and overcome on bone.

    abstract::Osteoclasts and macrophages share progenitors that must receive decisive lineage signals driving them into their respective differentiation routes. Macrophage colony stimulation factor M-CSF is a common factor; bone is likely the stimulus for osteoclast differentiation. To elucidate the effect of both, shared mouse bo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24702

    authors: De Vries TJ,Schoenmaker T,Aerts D,Grevers LC,Souza PP,Nazmi K,van de Wiel M,Ylstra B,Lent PL,Leenen PJ,Everts V

    更新日期:2015-01-01 00:00:00

  • Melatonin attenuates TNF-α-mediated hepatocytes damage via inhibiting mitochondrial stress and activating the Akt-Sirt3 signaling pathway.

    abstract::The role of mitochondrial dysfunction and its molecular mechanism in inflammation-induced acute liver failure (ALF) remain unknown. Despite the numerous studies performed to date, very few therapies are available for inflammation-induced ALF. Therefore, our study is aimed to explore the regulatory effects of mitochond...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28701

    authors: Song J,Lu C,Zhao W,Shao X

    更新日期:2019-11-01 00:00:00

  • Cucurbitacin B suppresses proliferation of pancreatic cancer cells by ceRNA: Effect of miR-146b-5p and lncRNA-AFAP1-AS1.

    abstract::Cucurbitacin B (CuB) is a natural tetracyclic triterpene product that displays antitumor activity against a wide variety of cancers. In this study, we explored the antipancreatic cancer activity of CuB via the inhibition of expression of the cancer-related long noncoding RNA, actin filament-associated protein 1-antise...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27264

    authors: Zhou J,Liu M,Chen Y,Xu S,Guo Y,Zhao L

    更新日期:2019-04-01 00:00:00

  • Combination of Rapamycin and Resveratrol for Treatment of Bladder Cancer.

    abstract::Loss of TSC1 function, a crucial negative regulator of mTOR signaling, is a common alteration in bladder cancer. Mutations in other members of the PI3K pathway, leading to mTOR activation, are also found in bladder cancer. This provides rationale for targeting mTOR for treatment of bladder cancer characterized by TSC1...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25443

    authors: Alayev A,Salamon RS,Schwartz NS,Berman AY,Wiener SL,Holz MK

    更新日期:2017-02-01 00:00:00

  • Ganoderma lucidum polysaccharides prevent platelet-derived growth factor-stimulated smooth muscle cell proliferation in vitro and neointimal hyperplasia in the endothelial-denuded artery in vivo.

    abstract::Ganoderma lucidum is used in traditional Chinese medicine to prevent or treat a variety of diseases, including cardiovascular disorders. We previously demonstrated that a glucan-containing extract of Reishi polysaccharides (EORP) has the potent anti-inflammatory action of reducing ICAM-1 expression in lipopolysacchari...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.23053

    authors: Wang SH,Liang CJ,Weng YW,Chen YH,Hsu HY,Chien HF,Tsai JS,Tseng YC,Li CY,Chen YL

    更新日期:2012-08-01 00:00:00

  • INI1 expression induces cell cycle arrest and markers of senescence in malignant rhabdoid tumor cells.

    abstract::The INI1 gene, which encodes a functionally uncharacterized protein component of the hSWI/SNF chromatin remodeling complex, is often mutated or deleted in malignant rhabdoid tumor (MRT). Two isoforms of INI1, that differ by the variable inclusion of nine amino acids, potentially are produced by differential RNA splici...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.10201

    authors: Reincke BS,Rosson GB,Oswald BW,Wright CF

    更新日期:2003-03-01 00:00:00

  • Decoupled syndecan 1 mRNA and protein expression is differentially regulated by angiotensin II in macrophages.

    abstract::It has been established that syndecan-1 is an important modulator of events relevant to acute tissue repair and chronic injury responses. The current studies were designed to examine syndecan-1 expression during atherosclerotic lesion formation and whether angiotensin II influences syndecan-1 expression in macrophages...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21271

    authors: Wang W,Haller CA,Wen J,Wang P,Chaikof EL

    更新日期:2008-03-01 00:00:00

  • Inhibitory effect of blue light emitting diode on migration and invasion of cancer cells.

    abstract::The aim of this study was to determine the effects and molecular mechanism of blue light emitting diode (LED) in tumor cells. A migration and invasion assay for the metastatic behavior of mouse colon cancer CT-26 and human fibrosarcoma HT-1080 cells was performed. Cancer cell migration-related proteins were identified...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25805

    authors: Oh PS,Kim HS,Kim EM,Hwang H,Ryu HH,Lim S,Sohn MH,Jeong HJ

    更新日期:2017-12-01 00:00:00

  • Nerve repair and regeneration: Biological tubulization limits and future perspectives.

    abstract::Peripheral nerve physiology and regeneration has been observed and investigated in literature but surgical applications to reconstruct and restore motor or sensory functions are still in a developmental phase. The peripheral nerve progresses slowly and incompletely compared with other tissues, it may provoke separatio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.27299

    authors: Riccio M,Marchesini A,Pugliese P,De Francesco F

    更新日期:2019-04-01 00:00:00

  • Pin1 plays a critical role as a molecular switch in canonical BMP signaling.

    abstract::Pin1 is a peptidyl prolyl cis-trans isomerase that specifically binds to the phosphoserine-proline or phosphothreonine-proline motifs of numerous proteins. Previously, we reported that Pin1 deficiency resulted in defects in osteoblast differentiation during early bone development. In this study, we found that adult Pi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24787

    authors: Yoon WJ,Islam R,Cho YD,Ryu KM,Shin HR,Woo KM,Baek JH,Ryoo HM

    更新日期:2015-03-01 00:00:00

  • Mechanisms of apoptosis modulation by curcumin: Implications for cancer therapy.

    abstract::Cancer incidences are growing and cause millions of deaths worldwide. Cancer therapy is one of the most important challenges in medicine. Improving therapeutic outcomes from cancer therapy is necessary for increasing patients' survival and quality of life. Adjuvant therapy using various types of antibodies or immunomo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.28122

    authors: Mortezaee K,Salehi E,Mirtavoos-Mahyari H,Motevaseli E,Najafi M,Farhood B,Rosengren RJ,Sahebkar A

    更新日期:2019-08-01 00:00:00

  • Synergism between gangliosides and basic fibroblastic growth factor in favouring survival, growth, and motility of capillary endothelium.

    abstract::The experiments reported were motivated by the observation that in vivo gangliosides promoted angiogenesis when the dose of the angiogenic factor was too low to be effective (Ziche et al.: Laboratory Investigation 61:629-634, 1989). As an approach to understanding the mechanism of this modulatory effect, we analysed t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041440319

    authors: De Cristan G,Morbidelli L,Alessandri G,Ziche M,Cappa AP,Gullino PM

    更新日期:1990-09-01 00:00:00

  • Synthetic pentapeptide from the B1 chain of laminin promotes B16F10 melanoma cell migration.

    abstract::Laminin is a basement membrane-specific glycoprotein that promotes cell adhesion, proliferation, differentiation, and tumor cell migration. Synthetic peptides from the amino acid sequence deduced from a cDNA clone of the B1 chain of laminin were tested for their ability to promote the migration of B16F10 melanoma cell...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041340216

    authors: Iwamoto Y,Graf J,Sasaki M,Kleinman HK,Greatorex DR,Martin GR,Robey FA,Yamada Y

    更新日期:1988-02-01 00:00:00

  • Bethanechol inhibition of chicken intestinal brush border Na/H exchange: role of protein kinase C and other calcium-dependent processes.

    abstract::Bethanechol, a muscarinic agonist, inhibits the initial rate of amiloride-sensitive Na uptake by intact mucosa of avian small intestine as well as by isolated chicken villus enterocytes, an effect that is maximal at 90 seconds and reverses by 6 minutes. Bethanechol similarly decreases intracellular pH in isolated cell...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041520218

    authors: Musch MW,Drabik-Arvans D,Rao MC,Chang EB

    更新日期:1992-08-01 00:00:00

  • Plasma membrane calcium ATPase 4 (PMCA4) co-ordinates calcium and nitric oxide signaling in regulating murine sperm functional activity.

    abstract::Reduced sperm motility (asthenospermia) and resulting infertility arise from deletion of the Plasma Membrane Ca2+ -ATPase 4 (Pmca4) gene which encodes the highly conserved Ca2+ efflux pump, PMCA4. This is the major Ca2+ clearance protein in murine sperm. Since the mechanism underlying asthenospermia in PMCA4's absence...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25882

    authors: Olli KE,Li K,Galileo DS,Martin-DeLeon PA

    更新日期:2018-01-01 00:00:00

  • Regulation of RNA polymerase II activity by CTD phosphorylation and cell cycle control.

    abstract::The carboxyl-terminal domain (CTD) of the largest subunit of mammalian RNA polymerase II (RNAP II) consists of 52 repeats of a consensus heptapeptide and is subject to phosphorylation and dephosphorylation events during each round of transcription. RNAP II activity is regulated during the cell cycle and cell cycle-dep...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.10058

    authors: Oelgeschläger T

    更新日期:2002-02-01 00:00:00

  • miR-124 promotes neural differentiation in mouse bulge stem cells by repressing Ptbp1 and Sox9.

    abstract::Hair follicle stem cells (HFSCs) are able to differentiate into neurons and glial cells. Distinct microRNAs (miRNAs) regulate the proliferation and differentiation of HFSCs. However, the exact role of miR-124 in the neural differentiation of HFSCs has not been elucidated. HFSCs were isolated from mouse whisker follicl...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27563

    authors: Mokabber H,Najafzadeh N,Mohammadzadeh Vardin M

    更新日期:2019-06-01 00:00:00

  • Characterizing the multiple roles of FGF-2 in SOD1G93A ALS mice in vivo and in vitro.

    abstract::We have previously shown that knockout of fibroblast growth factor-2 (FGF-2) and potential compensatory effects of other growth factors result in amelioration of disease symptoms in a transgenic mouse model of amyotrophic lateral sclerosis (ALS). ALS is a rapidly progressive neurological disorder leading to degenerati...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27498

    authors: Kefalakes E,Böselt S,Sarikidi A,Ettcheto M,Bursch F,Naujock M,Stanslowsky N,Schmuck M,Barenys M,Wegner F,Grothe C,Petri S

    更新日期:2019-05-01 00:00:00

  • A molecular mechanism of regulation for granulocyte macrophage colony stimulating factor from T lymphocytes.

    abstract::We have partially purified a mitogenic factor, termed colony stimulating factor, which is secreted from a human T lymphocytic cell line, and which stimulates in vitro formation of colonies of macrophages and granulocytes by human bone marrow cells. Colony stimulating factor (CSF) produced by cells grown in serum suppl...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041090309

    authors: Frölich M

    更新日期:1981-12-01 00:00:00

  • Role of RB and RB2/P130 genes in marrow stromal stem cells plasticity.

    abstract::Marrow stromal cells (MSCs) are stem-like cells having a striking somatic plasticity. In fact, besides differentiating into mesenchymal lineages (bone, cartilage, and fat), they are capable of differentiating into neurons and astrocytes in vitro and in vivo. The RB and RB2/P130 genes, belonging to the retinoblastoma g...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20026

    authors: Jori FP,Napolitano MA,Melone MA,Cipollaro M,Cascino A,Giordano A,Galderisi U

    更新日期:2004-08-01 00:00:00

  • Isolation and characterization of a cloned growth factor dependent macrophage cell line, BAC1.2F5.

    abstract::The SV40 transformed murine macrophage cell line, BAC1, proliferates in response to the colony stimulating factor, CSF-1 (Schwarzbaum et al., J. Immunol., 132:1158, 1984). In order to obtain a cell line suitable for biochemical and genetic studies of CSF-1 signal transduction, clones of BAC1 were established. Clones r...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041300316

    authors: Morgan C,Pollard JW,Stanley ER

    更新日期:1987-03-01 00:00:00

  • Hormonal responses to 1,25-dihydroxyvitamin D3 in cultured mouse osteoblast-like cells--modulation by changes in receptor level.

    abstract::The level of 1,25(OH)2D3 receptors in cultured mouse osteoblast-like (OB) cells is modulated by the rate of cell proliferation. We have studied two 1,25(OH)2D3-induced bioresponses to ascertain whether the changes in receptor levels during growth in culture alter cell responsiveness. Nuclear receptor levels were high ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041260104

    authors: Chen TL,Li JM,Ye TV,Cone CM,Feldman D

    更新日期:1986-01-01 00:00:00

  • Interactions Between Fatty Acid Transport Proteins, Genes That Encode for Them, and Exercise: A Systematic Review.

    abstract::Long-chain fatty acid (LCFA) movement into skeletal muscle involves a highly mediated process in which lipid rafts are utilized in the cellular membrane, involving numerous putative plasma membrane-associated LCFA transport proteins. The process of LCFA uptake and oxidation is of particular metabolic significance both...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.25281

    authors: Jayewardene AF,Mavros Y,Reeves A,Hancock DP,Gwinn T,Rooney KB

    更新日期:2016-08-01 00:00:00

  • Biosynthesis of proteoglycans by proliferating and differentiating normal human keratinocytes cultured in serum-free medium.

    abstract::Normal human keratinocytes (NHK) were cultured in serum-free medium, containing low (0.1 mM) or high (2 mM) calcium, to obtain proliferating and differentiating cultures, respectively. Proteoglycan (PG) synthesis of proliferating and differentiating NHK was investigated. Cultures were labeled with 35S-sulfate, and the...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041400113

    authors: Rahemtulla F,Moorer CM,Wille JJ Jr

    更新日期:1989-07-01 00:00:00

  • Chemically and virally transformed cells able to grow without anchorage in serum-free medium: evidence for an autocrine growth factor.

    abstract::BA10-IR transformed cells, obtained by treating Syrian hamster embryo fibroblasts (HEF) with 7-methylbenz(a)anthracene and cultivated for a long period, are highly tumorigenic and grow in suspension as aggregates (spheroids) (Levy et al., 1976). They also grow in attached form or as spheroids in serum-free (S-) synthe...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041310206

    authors: Xin LW,Jullien P,Lawrence DA,Pironin M,Vigier P

    更新日期:1987-05-01 00:00:00

  • The long noncoding RNA ZFAS1 promotes the progression of glioma by regulating the miR-150-5p/PLP2 axis.

    abstract::Numerous studies have reported that long noncoding RNA (lncRNA) dysregulation is involved in the progression of many malignant tumors, including glioma. The lncRNA ZNFX1 antisense RNA 1 (ZFAS1) plays an oncogenic role in various malignant tumors, such as gastric cancer and hepatocellular carcinoma. However, the underl...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29199

    authors: Li X,Luo Y,Liu L,Cui S,Chen W,Zeng A,Shi Y,Luo L

    更新日期:2020-03-01 00:00:00

  • Survivin modulatory role in autoimmune and autoinflammatory diseases.

    abstract::Baculoviral IAP repeat containing 5 (BIRC5) gene encodes the important protein as survivin, a multifunctional protein, which is involved in cellular and molecular networks, progression of cell cycle, homeostasis, developmental morphogenesis, and apoptosis. The proximal BIRC5 promoter possesses specific binding sites f...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28725

    authors: Pahlavan Y,Kahroba H,Samadi N,Karimi A,Ansarin K,Khabbazi A

    更新日期:2019-11-01 00:00:00

  • CCL18-dependent translocation of AMAP1 is critical for epithelial to mesenchymal transition in breast cancer.

    abstract::AMAP1 was a GTPase-activating protein that regulates cytoskeletal structures in focal adhesions, circular dorsal ruffles, and promote cell differentiation in tumor cells. But the activation and function of AMAP1 in breast cancer remain largely unexplored. Here we show that AMAP1 was phosphorylated and translocated to ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26164

    authors: Li H,Zhang D,Yu J,Liu H,Chen Z,Zhong H,Wan Y

    更新日期:2018-04-01 00:00:00

  • Enhanced islet expansion by beta-cell proliferation in young diabetes-prone rats fed a protective diet.

    abstract::Type 1 diabetes is inhibited in diabetes-prone BioBreeding (BBdp) rats fed a low-antigen hydrolyzed casein (HC) diet. In cereal-fed BBdp rats, islet expansion is defective accompanied by a futile upregulation of islet neogenesis without increased islet mass, due to a subtle blockage in islet cell cycle. We hypothesize...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22151

    authors: Wang GS,Kauri LM,Patrick C,Bareggi M,Rosenberg L,Scott FW

    更新日期:2010-08-01 00:00:00