16,16-Dimethyl Prostaglandin E2 modulation of endothelial monolayer paracellular barrier function.


:Prostaglandins prevent gastrointestinal mucosal injury and promote healing following mucosal injury by various noxious agents. Preservation or repair of microvascular function appears to be crucial in these processes. The processes involved in prostaglandin-mediated repair and preservation of endothelial function are unclear. In the present study, we investigated the role of prostaglandins on endothelial paracellular barrier function using the filter-grown bovine aortic endothelial cell monolayers. Endothelial paracellular barrier function as assessed using a paracellular marker, mannitol. Prostaglandin analogs 16,16-dimethyl prostaglandin E2 (DMPGE2) and prostaglandin I2 (PGI2) caused an enhancement of endothelial monolayer paracellular barrier function as evidenced by a dose-dependent decrease in endothelial paracellular permeability. DMPGE2 induced enhancement of endothelial paracellular barrier function correlated directly with increasing intracellular cAMP levels. Agents which increase intracellular cAMP levels at different stages of cAMP amplification cascade including phosphodiesterase inhibitor (3-isobutyl-1 methylxanthine [IBMX]), membrane permeable cAMP (8-bromo cAMP), and adenylate cyclase activators (isoproterenol and forskolin) also produced enhancement in endothelial paracellular barrier function. DMPGE2 enhancement of paracellular barrier function correlated with dense accumulation of actin microfilaments near the intercellular junctions. IBMX, isoproterenol, forskolin, and 8-bromo cAMP also produced similar changes in endothelial actin microfilaments. Cytochalasin B prevented the DMPGE2 enhancement of paracellular barrier function. Indomethacin (INDO), a cyclooxygenase inhibitor, caused a dose-dependent increase in endothelial paracellular permeability. Pharmacologic doses of INDO resulted in condensation and disruption of actin microfilaments with formation of large paracellular openings or gaps between the adjacent cells. Pretreatment of endothelial monolayers with DMPGE2 prevented INDO-induced disturbance of actin microfilaments and paracellular barrier function. IBMX, isoproterenol, forskolin, and 8-bromo cAMP also prevented INDO-induced changes in actin microfilaments and paracellular barrier function. These findings indicate that DMPGE2 has a paracellular barrier enhancing effect on filter-grown endothelial monolayers. This effect appears to be mediated through intracellular cAMP and actin microfilaments.


J Cell Physiol


Ma TY,Pedram A




Has Abstract


1996-05-01 00:00:00














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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Marx G,Blankenfeld A,Panet R,Gorodetsky R

    更新日期:1992-05-01 00:00:00

  • Evidence for poliovirus-induced cytoplasmic alkalinization in HeLa cells.

    abstract::During the early period after poliovirus infection of HeLa cells, cellular Na+/K+ ATPase activity is transiently activated. We investigated the possibility that Na+/K+ ATPase activation is a consequence of Na+/H+ antiporter activation. Increased uptake of the weak organic acid 5,5-dimethyloxazolidine-2,4-dione by infe...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Holsey C,Cragoe EJ Jr,Nair CN

    更新日期:1990-03-01 00:00:00

  • Hypoxia impairs cell fusion and differentiation process in human cytotrophoblast, in vitro.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Alsat E,Wyplosz P,Malassiné A,Guibourdenche J,Porquet D,Nessmann C,Evain-Brion D

    更新日期:1996-08-01 00:00:00

  • Effects of photoperiod on morphology and function in testis and epididymis of Cricetulus barabensis.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mou J,Xu J,Wang Z,Wang C,Yang X,Wang X,Xue H,Wu M,Xu L

    更新日期:2021-03-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Veronesi F,Tschon M,Visani A,Fini M

    更新日期:2019-12-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Han X,Chen H,Zhou J,Tai H,Gong H,Wang X,Huang N,Qin J,Fang T,Wang F,Xiao H

    更新日期:2018-05-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Alizadeh R,Fard ZA

    更新日期:2019-08-01 00:00:00

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    journal_title:Journal of cellular physiology

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    authors: Salehi A,Mobarhan MA,Mohammadi J,Shahsavarani H,Shokrgozar MA,Alipour A

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Massheimer V,Picotto G,Boland R,De Boland AR

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  • Protein kinase Cι expression and oncogenic signaling mechanisms in cancer.

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    pub_type: 杂志文章,评审


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    更新日期:2011-12-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Booth L,Roberts JL,Tavallai M,Nourbakhsh A,Chuckalovcak J,Carter J,Poklepovic A,Dent P

    更新日期:2015-08-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Puck TT,Erikson RL,Meek WD,Nielson SE

    更新日期:1981-06-01 00:00:00

  • Interleukin-6 increases adrenal androgen release by regulating the expression of steroidogenic proteins in NCI-H295R cells.

    abstract::The purpose of this study was to investigate the potential mechanism of interleukin-6 (IL-6) on the stimulation of excessive androgen secretion in human NCI-H295R adrenocortical cells. We performed transcriptome sequencing of cancer and paracancerous tissues obtained from functional adrenal cortical adenomas. The secr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wei X,Zhang J,Peng W,Xu H,Wei Z,Pang L,Liu J,Wang T

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    journal_title:Journal of cellular physiology

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    authors: Funanage VL,Smith SM,Minnich MA

    更新日期:1992-02-01 00:00:00

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    authors: Petre-Lazar B,Livera G,Moreno SG,Trautmann E,Duquenne C,Hanoux V,Habert R,Coffigny H

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    authors: Villalobos Campos S,Schönthal AH

    更新日期:2000-01-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Riaz S,Zeidan A,Mraiche F

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kloppel TM

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    journal_title:Journal of cellular physiology

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    authors: Ito K,Nakazato T,Miyakawa Y,Yamato K,Ikeda Y,Kizaki M

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    pub_type: 杂志文章


    authors: Li Z,Wang F,Zha S,Cao Q,Sheng J,Chen S

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Muscella A,Storelli C,Marsigliante S

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Guerne PA,Lotz M

    更新日期:1991-10-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sun NK,Kohli A,Huang SL,Chang TC,Chao CC

    更新日期:2019-06-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Pace E,Di Vincenzo S,Di Salvo E,Genovese S,Dino P,Sangiorgi C,Ferraro M,Gangemi S

    更新日期:2019-12-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Henderson JE,He B,Goltzman D,Karaplis AC

    更新日期:1996-10-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wei Y,Gong L,Fu W,Xu S,Wang Z,Zhang J,Ning E,Chang H,Wang H,Gao Y

    更新日期:2018-11-01 00:00:00

  • A 54-kDa short variant of DHX33 functions in regulating mRNA translation.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Su D,Yuan B,Su C,Zhang Y

    更新日期:2019-01-26 00:00:00