Mechanisms controlling neurite outgrowth in a pheochromocytoma cell line: the role of TRPC channels.


:Transient Receptor Potential Canonical (TRPC) channels are implicated in modulating neurite outgrowth. The expression pattern of TRPCs changes significantly during brain development, suggesting that fine-tuning TRPC expression may be important for orchestrating neuritogenesis. To study how alterations in the TRPC expression pattern affect neurite outgrowth, we used nerve growth factor (NGF)-differentiated rat pheochromocytoma 12 (PC12) cells, a model system for neuritogenesis. In PC12 cells, NGF markedly up-regulated TRPC1 and TRPC6 expression, but down-regulated TRPC5 expression while promoting neurite outgrowth. Overexpression of TRPC1 augmented, whereas TRPC5 overexpression decelerated NGF-induced neurite outgrowth. Conversely, shRNA-mediated knockdown of TRPC1 decreased, whereas shRNA-mediated knockdown of TRPC5 increased NGF-induced neurite extension. Endogenous TRPC1 attenuated the anti-neuritogenic effect of overexpressed TRPC5 in part by forming the heteromeric TRPC1-TRPC5 channels. Previous reports suggested that TRPC6 may facilitate neurite outgrowth. However, we found that TRPC6 overexpression slowed down neuritogenesis, whereas dominant negative TRPC6 (DN-TRPC6) facilitated neurite outgrowth in NGF-differentiated PC12 cells. Consistent with these findings, hyperforin, a neurite outgrowth promoting factor, decreased TRPC6 expression in NGF-differentiated PC12 cells. Using pharmacological and molecular biological approaches, we determined that NGF up-regulated TRPC1 and TRPC6 expression via a p75(NTR)-IKK(2)-dependent pathway that did not involve TrkA receptor signaling in PC12 cells. Similarly, NGF up-regulated TRPC1 and TRPC6 via an IKK(2) dependent pathway in primary cultured hippocampal neurons. Thus, our data suggest that a balance of TRPC1, TRPC5, and TRPC6 expression determines neurite extension rate in neural cells, with TRPC6 emerging as an NGF-dependent "molecular damper" maintaining a submaximal velocity of neurite extension.


J Cell Physiol


Kumar S,Chakraborty S,Barbosa C,Brustovetsky T,Brustovetsky N,Obukhov AG




Has Abstract


2012-04-01 00:00:00












  • Stimulation of LDL receptor activity in Hep-G2 cells by a serum factor(s).

    abstract::The regulation of low-density lipoprotein (LDL) receptor activity in the human hepatoma cell line Hep-G2 by serum components was examined. Incubation of dense monolayers of Hep-G2 cells with fresh medium containing 10% fetal calf serum (FM) produced a time-dependent increase in LDL receptor activity. Uptake and degrad...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ellsworth JL,Brown C,Cooper AD

    更新日期:1988-05-01 00:00:00

  • Characterization of the D-allose-mediated regulation of sugar transport in Chinese hamster fibroblasts.

    abstract::Exposure to D-allose has been demonstrated to lead to decreased 2-deoxy-D-glucose (2-DG) and 3-0-methyl-D-glucose transport in the V79 Chinese hamster lung fibroblast cell line. The effect of D-allose 1) was maximal after 4 hours exposure to the cells; 2) was optimal between 2.77 and 5.55 mM D-allose; and 3) led to a ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Germinario RJ,Kristof A,Chang Z,Manuel S

    更新日期:1990-11-01 00:00:00

  • Regulation of endothelin receptors by nitric oxide in cultured rat vascular smooth muscle cells.

    abstract::Two important mediators of endothelium-dependent regulation of vascular smooth muscle tone and proliferation are nitric oxide (NO) and endothelin (ET-1). An imbalance between NO and ET-1 may contribute to the alterations in vascular tone characteristic of cardiovascular disease. The objective of this study was to dete...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Redmond EM,Cahill PA,Hodges R,Zhang S,Sitzmann JV

    更新日期:1996-03-01 00:00:00

  • Long noncoding RNA MT1JP inhibits proliferation, invasion, and migration while promoting apoptosis of glioma cells through the activation of PTEN/Akt signaling pathway.

    abstract::This study is carried out to elucidate the role of long noncoding RNAs (lncRNAs) MT1JP in proliferation, invasion, migration, and apoptosis of glioma cells through the regulation of PTEN/Akt signaling pathway. The expression of MT1JP in 80 normal brain tissues and 138 glioma tissues, as well as glioma cell lines, was ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang Y,Sui R,Chen Y,Liang H,Shi J,Piao H

    更新日期:2019-11-01 00:00:00

  • Ascorbic Acid Induces Necrosis in Human Laryngeal Squamous Cell Carcinoma via ROS, PKC, and Calcium Signaling.

    abstract::Ascorbic acid induces apoptosis, autophagy, and necrotic cell death in cancer cells. We investigated the mechanisms by which ascorbic acid induces death in laryngeal squamous cell carcinoma Hep2 cells. Ascorbic acid markedly reduced cell viability and induced death without caspase activation and an increase in cytochr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Baek MW,Cho HS,Kim SH,Kim WJ,Jung JY

    更新日期:2017-02-01 00:00:00

  • Nexavar/Stivarga and viagra interact to kill tumor cells.

    abstract::We determined whether the multi-kinase inhibitor sorafenib or its derivative regorafenib interacted with phosphodiesterase 5 (PDE5) inhibitors such as Viagra (sildenafil) to kill tumor cells. PDE5 and PDGFRα/β were over-expressed in liver tumors compared to normal liver tissue. In multiple cell types in vitro sorafeni...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tavallai M,Hamed HA,Roberts JL,Cruickshanks N,Chuckalovcak J,Poklepovic A,Booth L,Dent P

    更新日期:2015-09-01 00:00:00

  • Effects of epoxyeicosatrienoic acids on 86Rb uptake in renal epithelial cells.

    abstract::Epoxyeicosatrienoic acids (EETs) are arachidonic acid metabolites formed endogenously via the cytochrome P450 pathway in rat, rabbit, and human kidney. We characterized the effects of the four regioisomeric EETs on ion transport in the renal epithelial cell line, LLC-PK1. Among the EETs, 14,15-EET was the most potent ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Staudinger R,Escalante B,Schwartzman ML,Abraham NG

    更新日期:1994-07-01 00:00:00

  • Extracellular high K+ stimulates vesicular glutamate release from astrocytes by activating voltage-dependent calcium channels.

    abstract::Extracellular high K(+) (75 mM) increased intracellular Ca(2+) concentrations in cultured rat hippocampal astrocytes, and the Ca(2+) rise was abolished by deleting extracellular Ca(2+) or cadmium, a non-selective inhibitor of voltage-dependent calcium channels (VDCCs). In the reverse transcription-polymerase chain rea...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yaguchi T,Nishizaki T

    更新日期:2010-11-01 00:00:00

  • Transmembrane protein 215 promotes angiogenesis by maintaining endothelial cell survival.

    abstract::Sprouting angiogenesis is a major form of neovascularization of tissues suffering from hypoxia and other related stress. Endothelial cells (ECs) undergo proliferation, differentiation, programmed death, and migration during angiogenic sprouting, but the underlying molecular mechanisms regulating ECs in angiogenesis ha...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Liu Y,Zheng Q,He G,Zhang M,Yan X,Yang Z,Zhang P,Wang L,Liu J,Liang L,Han H

    更新日期:2019-06-01 00:00:00

  • Defect in polyamine metabolism in a BHK cell mutant temperature-sensitive for rRNA maturation.

    abstract::A mutant of BHK cells (ts422E) temperature-sensitive for processing 32S rRNA to 28S rRNA (Toniolo et al., '73) also loses the ability to synthesize polyamines and 5.8S rRNA when shifted to the non-permissive temperature (39 degrees). The activity of several enzymes not involved with polyamine synthesis, methylation of...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Levin EG,Clark JL

    更新日期:1979-12-01 00:00:00

  • Teeth-derived stem cells: A source for cell therapy.

    abstract::Cell therapy is one of the important therapeutic approaches in the treatment of many diseases such as cancer, degenerative diseases, and cardiovascular diseases. Among various cell types, which could be used as cell therapies, stem cell therapy has emerged as powerful tools in the treatment of several diseases. Multip...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Baniebrahimi G,Khanmohammadi R,Mir F

    更新日期:2019-03-01 00:00:00

  • Macrophage plasticity, polarization, and function in health and disease.

    abstract::Macrophages are heterogeneous and their phenotype and functions are regulated by the surrounding micro-environment. Macrophages commonly exist in two distinct subsets: 1) Classically activated or M1 macrophages, which are pro-inflammatory and polarized by lipopolysaccharide (LPS) either alone or in association with Th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Shapouri-Moghaddam A,Mohammadian S,Vazini H,Taghadosi M,Esmaeili SA,Mardani F,Seifi B,Mohammadi A,Afshari JT,Sahebkar A

    更新日期:2018-09-01 00:00:00

  • Characterization of neutral amino acid uptake by cultured epithelial cells from pig kidney.

    abstract::Two transport systems for neutral amino acids have been characterised in LLC-PK, cells. The first, which transports alanine in a sodium-dependent manner, also mediates alanine exchange and is perferentially inhibited by serine, cysteine, and alpha-amino-n-butyric acid. This system resembles the ASC system in Ehrlich a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sepúlveda FV,Pearson JD

    更新日期:1982-08-01 00:00:00

  • Effect of Rooibos (Aspalathus linearis) extract on atorvastatin-induced toxicity in C3A liver cells.

    abstract::Rooibos (Aspalathus linearis) has various health benefits. Two case studies have associated chronic Rooibos consumption with conventional prescription medications, including atorvastatin (ATV), with hepatotoxicity. Statins act by inhibiting hydroxymethylglutaryl-coenzyme A reductase, a rate-limiting enzyme in choleste...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Millar DA,Bowles S,Windvogel SL,Louw J,Muller CJF

    更新日期:2020-12-01 00:00:00

  • Growth stimulation of colorectal carcinoma cells via the c-kit receptor is inhibited by TGF-beta 1.

    abstract::Activation of the receptor tyrosine kinase c-kit by the kit-ligand, also known as stem cell factor (SCF), is essential to melanocyte and germ cell development and during the early stages of hematopoiesis. Deregulated expression of c-kit has been reported in malignancies affecting these lineages, i.e., myeloid leukemia...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bellone G,Silvestri S,Artusio E,Tibaudi D,Turletti A,Geuna M,Giachino C,Valente G,Emanuelli G,Rodeck U

    更新日期:1997-07-01 00:00:00

  • The rate of fluid shear stress is a potent regulator for the differentiation of mesenchymal stem cells.

    abstract::We have previously demonstrated that the rate of fluid shear stress (ΔSS) can manipulate the fate of mesenchymal stem cells (MSCs) to osteogenic or chondrogenic cells. However, whether ΔSS is comparable to other two means of induction medium and substrate stiffness that have been proven to be potent in differentiation...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yue D,Zhang M,Lu J,Zhou J,Bai Y,Pan J

    更新日期:2019-02-19 00:00:00

  • Sarcolectin and interferon in the regulation of cell growth.

    abstract::Sarcolectin is an endolectin present in a great variety of conjunctival tissues (muscles, cartilage, sarcomas), but also in brain or placental extracts of vertebrates, including primates. When purified to electrophoretical homogeneity as a 65-kd protein, it agglutinates cells and has an affinity for simple sugars. In ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chany-Fournier F,Jiang PH,Chany C

    更新日期:1990-10-01 00:00:00

  • Antibodies reacting to mimotopes of Simian virus 40 large T antigen, the viral oncoprotein, in sera from children.

    abstract::Recent data indicate that the Simian virus 40 (SV40) infection appears to be transmitted in humans independently from early SV40-contaminated antipolio vaccines. Serum antibodies against SV40 large T antigen (Tag) were analyzed in children/adolescents and young adults. To investigate antibodies reacting to SV40 Tag an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mazzoni E,Frontini F,Rotondo JC,Zanotta N,Fioravanti A,Minelli F,Torreggiani E,Campisciano G,Marcuzzi A,Guerra G,Tommasini A,Touzé A,Martini F,Tognon M,Comar M

    更新日期:2019-04-01 00:00:00

  • Temporal studies on the tissue compartmentalization of bone sialoprotein (BSP), osteopontin (OPN), and SPARC protein during bone formation in vitro.

    abstract::To study the role of noncollagenous proteins in bone formation, the synthesis and tissue distribution of BSP (bone sialoprotein), OPN (osteopontin) and SPARC (secreted protein acidic and rich in cysteine) were analyzed using pulse-chase and continuous labeling protocols during bone formation by cultures of rat calvari...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kasugai S,Nagata T,Sodek J

    更新日期:1992-09-01 00:00:00

  • Calcium uptake by isolated rat intestinal cells.

    abstract::Intestinal cells were isolated by a combination of mechanical and enzymatic means, and their calcium uptake was assayed by a rapid filtration procedure. Calcium uptake was a time- and concentration-dependent process that was markedly elevated at 25 and 37 degrees C, as compared to 0 degree C. Cells isolated from rat d...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bronner F,Pansu D,Bosshard A,Lipton JH

    更新日期:1983-09-01 00:00:00

  • Flavonoid genistein protects bone marrow sinusoidal blood vessels from damage by methotrexate therapy in rats.

    abstract::Cancer chemotherapy can cause significant damage to the bone marrow (BM) microvascular (sinusoidal) system. Investigations must now address whether and how BM sinusoidal endothelial cells (SECs) can be protected during chemotherapy. Herein we examined the potential protective effects of genistein, a soy-derived flavon...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hassanshahi M,Su YW,Khabbazi S,Fan CM,Chen KM,Wang JF,Qian A,Howe PR,Yan DW,Zhou HD,Xian CJ

    更新日期:2019-07-01 00:00:00

  • Bethanechol inhibition of chicken intestinal brush border Na/H exchange: role of protein kinase C and other calcium-dependent processes.

    abstract::Bethanechol, a muscarinic agonist, inhibits the initial rate of amiloride-sensitive Na uptake by intact mucosa of avian small intestine as well as by isolated chicken villus enterocytes, an effect that is maximal at 90 seconds and reverses by 6 minutes. Bethanechol similarly decreases intracellular pH in isolated cell...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Musch MW,Drabik-Arvans D,Rao MC,Chang EB

    更新日期:1992-08-01 00:00:00

  • The competing endogenous circular RNA ADAMTS14 suppressed hepatocellular carcinoma progression through regulating microRNA-572/regulator of calcineurin 1.

    abstract::Emerging evidence have discovered that circular RNAs (circRNAs) may serve as diagnostic or tumor promising biomarkers. This study aimed to investigate how circular RNA ADAMTS14 (circADAMTS14) regulates microRNA-572/ regulator of calcineurin 1(miR-572/ RCAN1) in hepatocellular carcinoma (HCC). The expression profiles o...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Song C,Li D,Liu H,Sun H,Liu Z,Zhang L,Hu Y

    更新日期:2019-03-01 00:00:00

  • A novel chemically defined serum- and feeder-free medium for undifferentiated growth of porcine pluripotent stem cells.

    abstract::Development and improvement of in vitro culture system supporting self-renewal and unlimited proliferation of porcine pluripotent stem cells (pPSCs) is an indispensable process for the naïve pPSCs establishment. In this study, we modified the previous culture system and attempted to develop a novel chemically defined ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang X,Xue B,Li Y,Wei R,Yu Z,Jin J,Zhang Y,Liu Z

    更新日期:2019-01-30 00:00:00

  • Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K/Akt and MAPK signaling pathways in RAW264.7 cells.

    abstract::Ganoderma lucidum immunomodulatory protein (FIP-glu) is an active ingredient with potential immunoregulatory functions. The study was conducted to explore the immunomodulatory activities of recombinant FIP-glu (rFIP-glu) and its possible mechanism in macrophage RAW264.7 cells. In vitro assays of biological activity in...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Li QZ,Chang YZ,He ZM,Chen L,Zhou XW

    更新日期:2019-12-01 00:00:00

  • PIWI-interacting RNA 39980 promotes tumor progression and reduces drug sensitivity in neuroblastoma cells.

    abstract::Neuroblastoma (NB) is the leading pediatric cancer known for its heterogeneity and clinical aggressiveness leading to chemoresistance. Recent evidence in small RNA research has led to the discovery of PIWI-interacting RNAs (piRNAs) which work in an orchestrated fashion to modulate gene expression both in homeostatic c...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Roy J,Das B,Jain N,Mallick B

    更新日期:2020-03-01 00:00:00

  • c-Src kinase contributes on endothelial cells mechanotransduction in a heat shock protein 70-dependent turnover manner.

    abstract::Shear stress changes are associated with a repertory of signaling cascade modulating vascular phenotype. As shear stress-related tensional forces might be associated with pathophysiological susceptibility, a more comprehensive molecular map needs to be addressed. Thus, we subjected human umbilical vein endothelial cel...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Pinto TS,Fernandes CJDC,da Silva RA,Gomes AM,Vieira JCS,Padilha PM,Zambuzzi WF

    更新日期:2019-07-01 00:00:00

  • Production of growth factors by type 5 adenovirus transformed rat embryo cells.

    abstract::Transformation of Sprague-Dawley rat embryo (RE) cells and a cloned Fischer rat embryo cell line (CREF) with wild-type (Ad5) or a temperature-sensitive DNA-minus mutant (H5ts125) of type 5 adenovirus results in a reduction in binding of epidermal growth factor (EGF) to cell surface receptors. A reduction in EGF bindin...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Fisher PB,Boersig MR,Graham GM,Weinstein IB

    更新日期:1983-03-01 00:00:00

  • Archetypal autophagic players through new lenses for bone marrow stem/mature cells regulation.

    abstract::The bone marrow landscape consists of specialized and stem/progenitor cells, which coordinate important tissue-related and systemic physiological features. Within the marrow cavity, stem/progenitor and differentiated hematopoietic and skeletal cells congregate into dynamic functional assemblies throughout specific ana...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Agas D,Sabbieti MG

    更新日期:2021-01-25 00:00:00

  • Preparation of superparamagnetic iron oxide/doxorubicin loaded chitosan nanoparticles as a promising glioblastoma theranostic tool.

    abstract::Theranostic nanoparticles (NPs) are promising for opening new windows toward personalized disease management. Using a single particle capable of both diagnosis and drug delivery, is the major benefit of such particles. In the present study, chitosan NPs were used as a dual action carrier for doxorubicin (DOX; chemothe...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Gholami L,Tafaghodi M,Abbasi B,Daroudi M,Kazemi Oskuee R

    更新日期:2019-02-01 00:00:00