Abstract：
:The cardiac glyoside, ouabain, normally kills HeLa cells at concentrations of about 10-7 M or greater. By treating a population of HeLa cells with increasingly higher concentrations of the drug, a vaiant population was obtained of HeLa cells capable of growing in medium containing 10-4 M ouabain. Inhibition of volume regulation of cells subjected to hypotonic shock was used as a measure of inhibition of active transport of Na across the plasma membrane. In that way of dose-response curves for the rapid effects of ouabain and other inhibitors of active Na transport were obtained with both the original, ouabain-sensitive (OS) and the variant, ouabain-resistant (OR) cells. Three other cardiac glycosides (digoxin, digtoxin and hellebrin) and two aglycones (digitoxigenin and strophanthidin) were found to be equally as effective as ouabain in inhibiting volume regulation of the OS cells; the concentration whichproduced half-maximum inhibition, I(max/2), was about 6X 10-7 M in each case. Similar inhibition of the OR population by ouabain was observed only when the concentration exceeded 10-4 M [I(max/2-2.5 X 10-4 M], and the other steroid compounds had no effect on the variant cells at the highest concentrations tested (-2 X 10-5 M). OR and OS cells different also in their sensitivities to its cardoactive erythrophleum alkaloid, coumingine; I(max/2) for OS and OR cells was 5 X 10-8 M and 6 X 10-7 M, respectively. These results in addition to results of ouabain binding experiments and measurements of the rates of reversal of inhibition of volume regulation, suggest that a major reason for the differential sensitivities of the two phenotypes to these drugs is different affinities of their sodium pumps for inhibitors of active transport.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Rosenberg HMdoi
10.1002/jcp.1040850114keywords:
subject
Has Abstractpub_date
1975-02-01 00:00:00pages
135-42issue
1eissn
0021-9541issn
1097-4652journal_volume
85pub_type
杂志文章abstract：:Interleukin (IL)-8 is released both in visceral adipose tissue and in contracting skeletal muscles. In this study, we examined cellular pathways associated with muscle hypertrophy, chosen on the basis of microRNA profiling, in differentiating rat primary skeletal muscle cells (RSkMC) treated with IL-8 (1 ng/ml) for 11...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28568
更新日期：2019-11-01 00:00:00
abstract：:We have previously shown that knockout of fibroblast growth factor-2 (FGF-2) and potential compensatory effects of other growth factors result in amelioration of disease symptoms in a transgenic mouse model of amyotrophic lateral sclerosis (ALS). ALS is a rapidly progressive neurological disorder leading to degenerati...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27498
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abstract：:The significance of low pH-induced stimulation of osteoclastic bone resorption has recently been questioned following the finding that embryonic chick osteoclasts were only weakly stimulated by extremely low pH (6.5) and that the effect was transient, apparently due to cytotoxicity. Although low pH in the range 6.8-7....
journal_title：Journal of cellular physiology
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更新日期：1993-03-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25451
更新日期：2017-01-01 00:00:00
abstract：:Keratin intermediate filaments play an important role in maintaining the integrity of the skin structure. Understanding the importance of this subject is possible with the investigation of keratin defects in epidermolysis bullosa simplex (EBS). Nowadays, in addition to clinical criteria, new molecular diagnostic metho...
journal_title：Journal of cellular physiology
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journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
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更新日期：2020-07-01 00:00:00
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journal_title：Journal of cellular physiology
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：1993-11-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
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更新日期：2014-01-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041170314
更新日期：1983-12-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：2018-04-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：2011-03-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：2019-05-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041240213
更新日期：1985-08-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：2014-04-01 00:00:00
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journal_title：Journal of cellular physiology
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doi：10.1002/jcp.20613
更新日期：2006-06-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：2020-08-31 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：2018-12-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
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更新日期：2019-06-01 00:00:00
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journal_title：Journal of cellular physiology
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journal_title：Journal of cellular physiology
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更新日期：1977-02-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：2019-11-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：2005-12-01 00:00:00
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更新日期：2003-08-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199807)176:1<10::AID-JCP2>
更新日期：1998-07-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040920308
更新日期：1977-09-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：1979-07-01 00:00:00
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更新日期：2018-01-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：1998-05-01 00:00:00
