C-C motif chemokine ligand 23 abolishes ER stress- and LPS-induced reduction in proliferation of bovine endometrial epithelial cells.

Abstract:

:To reduce embryonic losses in domestic animals for economic production of livestock meat and milk, chemokines and their receptors are required for proper implantation and placentation during early pregnancy. In this study, we investigated the effects of C-C-motif chemokine ligand 23 (CCL23) on the proliferation of bovine endometrial (BEND) epithelial cells. CCL23 treatment improved BEND cell proliferation by enhancing PCNA and cyclin D1 expression via activation of the PI3K/AKT and MAPK signaling pathways. In addition, a combination of CCL23 and tunicamycin reversed the ER stress-induced reduction in cell proliferation and the decreased expression of UPR-mediated signaling proteins, including IRE1α, PERK, and ATF6α. Moreover, it regulated the lipopolysaccharide-induced inflammation in BEND cells by inhibiting the expression of pro-inflammatory cytokines (IL-6 and IL-8), and by restoring intracellular Ca2+ levels. These findings demonstrate that CCL23 improves endometrial development and uterine receptivity required for implantation and placentation during early pregnancy.

journal_name

J Cell Physiol

authors

Lim W,Bae H,Bazer FW,Song G

doi

10.1002/jcp.26210

subject

Has Abstract

pub_date

2018-04-01 00:00:00

pages

3529-3539

issue

4

eissn

0021-9541

issn

1097-4652

journal_volume

233

pub_type

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