Abstract:
:Protein-DNA interactions within the promoter of a cell cycle-regulated human H4 histone gene were examined by binding of 5'-end-labeled DNA segments to Western blots of nuclear protein fractions. Specific protein interactions were observed with DNA segments located between -500 bp and -1,070 bp upstream of the ATG initiation codon and included a histone H1 binding segment flanked on both sides by binding sites for a 45 kD nuclear protein. This region of the gene contains a DNase I-sensitive site in the center (-720 to -820 bp), and sequence analysis revealed the presence of scaffold attachment sequences in the two flanking segments. Topoisomerase II consensus sequences and in vitro topoisomerase II cleavage sites were also detected in the two flanking segments. Our results suggest that the 45 kd nuclear protein may preferentially interact with these two segments of the H4 histone gene to mediate association with the nuclear matrix. The presence of negative regulatory elements in this putative matrix attachment region provides a basis for the speculation that such nuclear proteins are associated with alterations in gene-matrix interaction that are functionally related to gene expression.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Pauli U,Chiu JF,Ditullio P,Kroeger P,Shalhoub V,Rowe T,Stein G,Stein Jdoi
10.1002/jcp.1041390214subject
Has Abstractpub_date
1989-05-01 00:00:00pages
320-8issue
2eissn
0021-9541issn
1097-4652journal_volume
139pub_type
杂志文章abstract::Nowadays, epigenetics covers a crucial role in different fields of science. The enhancer of zeste homolog 2 (EZH2), the catalytic subunit of the Polycomb Repressive Complex 2 (PRC2), is a big proponent of how epigenetic changes can affect the initiation and progression of several diseases. Through its catalytic activi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.26545
更新日期:2018-09-01 00:00:00
abstract::Multiple modalities for lung cancer therapy have emerged in the past decade, whereas their clinical applications and survival-beneficiary is little known. Vaccination with dendritic cells (DCs) or DCs/cytokine-induced killer (CIK) cells has shown limited success in the treatment of patients with advanced non-small-cel...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28977
更新日期:2020-01-01 00:00:00
abstract::This investigation was undertaken to determine whether primitive stem cells and/or fully differentiated macrophages were the source of in vitro colonies derived from hematopoietic tissues. The chicken colony-forming cell (CFC) present in uncultured yolk sac was a nonadherent, presumably undifferentiated cell. The effi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040850104
更新日期:1975-02-01 00:00:00
abstract::The significance of low pH-induced stimulation of osteoclastic bone resorption has recently been questioned following the finding that embryonic chick osteoclasts were only weakly stimulated by extremely low pH (6.5) and that the effect was transient, apparently due to cytotoxicity. Although low pH in the range 6.8-7....
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041540309
更新日期:1993-03-01 00:00:00
abstract::Testicular germ cell tumors (TGCTs) are frequent solid malignant tumors and cause of death in men between 20-40 years of age. Genetic and environmental factors play an important role in the origin and development of TGCTs. Although the majority of TGCTs are responsive to chemotherapy, about 20% of patient presents inc...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.25482
更新日期:2017-02-01 00:00:00
abstract::Autocrine activation of the epidermal growth factor (EGF) receptor on keratinocytes has been recognized as an important growth regulatory mechanism involved in epithelial homeostasis, and, possibly, hyperproliferative diseases. Insulin-like growth factor (IGF)-1 and insulin have been shown to be paracrine keratinocyte...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041630206
更新日期:1995-05-01 00:00:00
abstract::Previous studies have indicated that intracellular Ca2+ is involved in fetal bovine serum (FBS)- or growth factor (GF)-stimulated Na+ influx in human foreskin fibroblasts (HSWP). In the present study, 45Ca2+ efflux from serum-deprived HSWP cells was measured in response to 10% FBS or GF [lys-bradykinin, vasopressin, e...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041170105
更新日期:1983-10-01 00:00:00
abstract::Dermal fibroblasts exposed to low oxygen tension show upregulated synthesis of transforming growth factor-beta 1 (TGF-beta 1), an established stimulatory peptide in the formation of extracellular matrix proteins. In this report, procollagen synthesis was measured in cultures of confluent adult human dermal fibroblasts...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041570225
更新日期:1993-11-01 00:00:00
abstract::Regulation of lipid synthesis from acetate in human diploid fibroblast cultures has been studied at various passage levels and at different stages of cell growth. When cultures were transferred to lipid free medium, a stimulation of [14C]acetate incorporation into lipid occurred within three to six hours after removal...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040890215
更新日期:1976-10-01 00:00:00
abstract::Cholesterol is an important component for cell physiology. It regulates the fluidity of cell membranes and determines the physical and biochemical properties of proteins. In the central nervous system, cholesterol controls synapse formation and function and supports the saltatory conduction of action potential. In rec...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.25488
更新日期:2017-02-01 00:00:00
abstract::There is evidence that cadmium can initiate carcinogenesis. However, the underlying mechanisms remain unknown. There is also evidence that moderate centrosome amplification can initiate tumorigenesis. The present study investigated whether cadmium could trigger cell centrosome amplification, and examined the underlyin...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28455
更新日期:2019-08-01 00:00:00
abstract::There is strong evidence that thyroid hormones through triiodothyronine (T3) regulate Sertoli cell proliferation and differentiation in the neonatal testis. However, the mechanism(s) by which they are able to control Sertoli cell proliferation is unclear. In the present study in vivo approaches (PTU-induced neonatal h...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20716
更新日期:2006-10-01 00:00:00
abstract::Glioblastoma (GBM) is the most common, malignant, and deadly primary glioma. Six-transmembrane epithelial antigen of prostate (STEAP) family is involved in tumorigenesis; here, we have explored the biological function and the prognostic value of the STEAP family in GBM. Differentially expressed STEAP genes in tumor an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30060
更新日期:2020-09-23 00:00:00
abstract::Pulmonary fibrosis is a potentially fatal consequence of treatments for malignancy and is an increasing problem in bone marrow transplant patients and in cases of allogenic lung transplant. The fibrotic response is characterized by increases in lung fibroblast number and collagen synthesis. This laboratory previously ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199605)167:2<290::AID-JCP1
更新日期:1996-05-01 00:00:00
abstract::The protein tyrosine kinase pp125FAK (focal adhesion kinase, or FAK) is expressed by a variety of cell types and has been implicated in integrin-mediated signaling events. We explored the potential functions of FAK by expressing it de novo in a cell type lacking FAK. We showed previously that cultured human macrophage...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199902)178:2<164::AID-JCP5
更新日期:1999-02-01 00:00:00
abstract::The mechanisms by which mature myeloid cells are released from the bone marrow into the peripheral blood are not clearly understood. Glycosylation is likely to play an important role, as has been shown in the homing of lymphocytes to lymph nodes and of neutrophils to inflamed endothelia. Cell surface sialylation is an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199906)179:3<315::AID-JCP9
更新日期:1999-06-01 00:00:00
abstract::Syndecans (SDCs) are a family of heparan sulfate proteoglycans (HSPGs) glycoproteins ubiquitously expressed on the cell surfaces and extracellular matrix of all mammalian tissues. There are four mammalian syndecans, SDC-1 thorough 4, which play a critical role in cell adhesion, migration, proliferation, differentiatio...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.26388
更新日期:2018-09-01 00:00:00
abstract::Anti-angiogenic agents regulate tumor growth by inhibiting endothelial cell proliferation and invasion. Carboxyamido-triazole (CAI), an inhibitor of non-voltage-operated calcium entry and calcium influx-mediated pathways, has angiogenesis and invasion inhibitory activity. We hypothesized that CAI may express its anti-...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10350
更新日期:2003-10-01 00:00:00
abstract::Low-power laser irradiation (LPLI) has been shown to exert promotive effects on cell survival and proliferation through activation of various signaling pathways. Estrogen receptors (ERs, ERα, and ERβ) are ligand-activated transcription factors, which regulate target gene expression, promote cell proliferation, and res...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24252
更新日期:2013-05-01 00:00:00
abstract::A proliferation inducing ligand (APRIL or TALL-2 and TRDL-1) was first discovered as a cytokine over-expressed in many transformed cells and with the capacity to stimulate proliferation. APRIL was shown to bind two different receptors of the TNF receptor superfamily: B cell maturation antigen (BCMA) and transmembrane ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.21561
更新日期:2009-01-01 00:00:00
abstract::The present study uses the osteoclast precursor clonal line, HD-11EM, to study the potential of hydrogen peroxide (H2O2) in mediating the differentiation of HD-11EM into osteoclast-like cells. HD-11EM cells are a newly established clonal cell line that, in response to 1alpha,25-(OH)2D3, differentiate into osteoclast-l...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199809)176:3<574::AID-JCP1
更新日期:1998-09-01 00:00:00
abstract::The herbicide 2,4,5 trichlorophenoxy acetic acid (245T) at concentrations from 0.5 to 0.9 mM, was found to inhibit respiration and then growth in exponentially growing cultures of Tetrahymena pyriformis. Cell division was stopped for periods up to 60 minutes after which the cells recovered and division resumed. Recove...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040850219
更新日期:1975-04-01 00:00:00
abstract::Insulin gene expression in rat insulinoma (RIN) cells is extinct in RIN x fibroblast hybrids and can reappear upon loss of DNA contributed by the fibroblast parent. (Besnard et al., Exp. Cell Res. 185:101-108, 1989). In the present study, we looked for the role of 5'-flanking sequences of the human insulin gene in the...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041460303
更新日期:1991-03-01 00:00:00
abstract::It is well known that cell proliferation (and hence, DNA synthesis) declines in human diploid fibroblast-like cells with increasing passage number. It is not clear whether DNA synthesis declines in the remaining cells that are still actively proliferating. Estimations of cell kinetic parameters permitted extrapolation...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041240126
更新日期:1985-07-01 00:00:00
abstract::The differentiation of human keratinocytes in most culture systems is incomplete; e.g., lamellar bodies, the characteristic lipid-delivery organelles of epidermis, are not present. Moreover, their lipid profile does not reflect the distinctive composition found in cornifying epidermis. In contrast, keratinocytes that ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041360113
更新日期:1988-07-01 00:00:00
abstract::Phospholipase C-ε (PLCε) is frequently overexpressed in tumors and plays an important role in the regulation of tumorigenesis. Although great progress has been made in understanding biological roles of PLCε, the relevant molecular mechanisms underlying its pro-tumor activity remain largely unclear. Here, we demonstrat...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29698
更新日期:2020-11-01 00:00:00
abstract::The function of microRNA-27a (miR-27a) on synovial angiogenesis and chondrocyte apoptosis in rats with knee osteoarthritis (KOA) by targeting PLK2 is explored in this present study. The rat model of KOA was conducted by anterior cruciate ligament transection. Rats were injected with miR-27a mimics, mimics NC, pcDNA3.1...
journal_title:Journal of cellular physiology
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doi:10.1002/jcp.28858
更新日期:2019-12-01 00:00:00
abstract::The increased levels of c-Myc protein observed previously in an ovarian carcinoma cell line stably transfected to express HER2 has suggested a role for the HER2 pathway in c-Myc expression. Analysis of HER2-transfected cells stimulated with heregulin beta1 (HRG) revealed increased c-Myc protein levels but not a corres...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20012
更新日期:2004-07-01 00:00:00
abstract::The synthesis and degradation of pectoralis myosin have been investigated in chickens 15-23 days after hatching. An essentially nonreutilizable tracer amino acid was used to demonstrate differences in the rates of synthesis and degradation of myosin isolated from normal and hypertrophied, cystrophic breast tissue. The...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040860406
更新日期:1975-10-01 00:00:00
abstract::As a natural metabolite of limonoids from Dictamnus dasycarpus, fraxinellone has been reported to be neuroprotective and anti-inflammatory. However, its influence on cellular metabolism remains largely unknown. In the present study, we investigated the effect of fraxinellone on cellular senescence-induced by oxidative...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26169
更新日期:2018-05-01 00:00:00