Abstract:
:Pulmonary fibrosis is a potentially fatal consequence of treatments for malignancy and is an increasing problem in bone marrow transplant patients and in cases of allogenic lung transplant. The fibrotic response is characterized by increases in lung fibroblast number and collagen synthesis. This laboratory previously isolated stable, functionally distinct, murine lung fibroblast subsets (Thy-1+ and Thy-1-) to study the contribution of fibroblast subpopulations in lung fibrosis. The fibroblast fibrotic response may be induced by cytokines secreted by infiltrating cells such as T lymphocytes and mast cells. In the current study two key regulatory cytokines, interferon-gamma (IFN-gamma) and interleukin-4 (IL-4), were investigated for their effects on the collagen synthesis of murine lung fibroblast subsets. IL-4 and IFN-gamma are putatively characterized as fibrogenic and anti-fibrogenic cytokines, respectively, and are found in repairing lung tissue. Stimulation with recombinant IL-4 induced a100% increase in total collagen production only by Thy-1+ fibroblasts. Types I and III collagen mRNA were increased in the Thy-1+ fibroblasts, unlike the Thy-1- subset. In contrast, IFN-gamma decreased constitutive collagen production by more than 50% in Thy-1+ and Thy-1- fibroblasts. Interestingly, the two subsets utilized their collagen production machinery (collagenase, tissue inhibitors of metalloproteinases) differently to further regulate collagen turnover in response to IL-4 and IFN-gamma. Overall, our data support the hypothesis that IL-4 is fibrogenic and IFN-gamma is anti-fibrogenic. Moreover, selective expansion of IL-4 responsive fibroblasts (e.g., Thy-1+) may be important in the transition from repair to chronic fibrosis. In addition, these data suggest that an inflammatory response dominated by IL-4-producing Th2 lymphocytes and/or mast cells will promote fibrosis development.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Sempowski GD,Derdak S,Phipps RPdoi
10.1002/(SICI)1097-4652(199605)167:2<290::AID-JCP1subject
Has Abstractpub_date
1996-05-01 00:00:00pages
290-6issue
2eissn
0021-9541issn
1097-4652pii
10.1002/(SICI)1097-4652(199605)167:2<290::AID-JCP1journal_volume
167pub_type
杂志文章abstract::Osteoclasts and macrophages share progenitors that must receive decisive lineage signals driving them into their respective differentiation routes. Macrophage colony stimulation factor M-CSF is a common factor; bone is likely the stimulus for osteoclast differentiation. To elucidate the effect of both, shared mouse bo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24702
更新日期:2015-01-01 00:00:00
abstract::Mesenchymal stromal cells (MSCs) have been widely exploited for the treatment of several conditions due to their intrinsic regenerative and immunomodulatory properties. MSC have demonstrated to be particularly relevant for the treatment of ischemic diseases, where MSC-based therapies can stimulate angiogenesis and ind...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29622
更新日期:2020-10-01 00:00:00
abstract::Transformation of Sprague-Dawley rat embryo (RE) cells and a cloned Fischer rat embryo cell line (CREF) with wild-type (Ad5) or a temperature-sensitive DNA-minus mutant (H5ts125) of type 5 adenovirus results in a reduction in binding of epidermal growth factor (EGF) to cell surface receptors. A reduction in EGF bindin...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041140315
更新日期:1983-03-01 00:00:00
abstract::Nonalcoholic fatty liver disease (NAFLD) is prevalent chronic liver diseases with unknown mechanism and no curative treatment. Hepatokines have demonstrated importance in NAFLD but, role of selenoprotein P (SeP) in NAFLD is unknown. A total of 79 patients with NAFLD and 79 healthy controls were included in this case-c...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30121
更新日期:2020-10-22 00:00:00
abstract::In this study, we used a bioinformatics approach to analyze the nucleotide composition and pattern of synonymous codon usage in mitochondrial ND genes in three amphibian groups, that is, orders Anura, Caudata, and Gymnophiona to identify the commonality and the differences of codon usage as no research work was report...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30050
更新日期:2020-09-22 00:00:00
abstract::Considerable evidence has accumulated for a role of a nonsteroidal mevalonate product in the regulation of DNA replication and cell division. We report here a similar requirement for mevalonate in a nonreplicative function, that of natural killer (NK) cell cytotoxicity. Treatment of NK cells with 10 microM compactin f...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390314
更新日期:1989-06-01 00:00:00
abstract::Activation of the epidermal growth factor receptor (EGFR) has been shown to occur by ligand-dependent and ligand-independent mechanisms. Different molecular mechanisms have been found to be responsible for ligand-independent receptor transactivation. Here, we show that hyperosmolar concentrations of sorbitol activate ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10134
更新日期:2002-08-01 00:00:00
abstract::CSF-1 is a hemopoietic growth factor that specifically regulates the survival, proliferation, and differentiation of mononuclear phagocytic cells. Populations of adherent bone marrow-derived macrophages (BMM) devoid of CSF-1 producing cells were used to study regulation by CSF-1 of macrophage entry into S phase. More ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041220210
更新日期:1985-02-01 00:00:00
abstract::Exposure of live cells to shear flow induces major changes in cell shape, adhesion to the extracellular matrix, and migration. In the present study, we show that exposure of cultured multiple myeloma (MM) cells to shear flow of 4-36 dynes/cm(2) triggers the extension of long tubular protrusions (denoted flow-induced p...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22680
更新日期:2011-12-01 00:00:00
abstract::A sensitive serum-free culture system was used to demonstrate that cells derived from normal human skin release soluble mediators which can modulate keratinocyte, melanocyte, and fibroblast growth in vitro. In M199 supplemented with epidermal growth factor, hydrocortisone, insulin, transferrin, triidothyronine, bovine...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041170215
更新日期:1983-11-01 00:00:00
abstract::The genetic factors known to be involved in the final realization of beta-glucuronidase activity in mice are considered from the standpoint of structural genes determining the catalytic activity of enzyme molecules as well as the recognition features of enzyme molecules that identify them for subsequent processing by ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040850406
更新日期:1975-04-01 00:00:00
abstract::The continuous discovery of human mesenchymal stem cells (hMSCs) in different tissues is stirring up a tremendous interest as a cell source for regenerative medicine therapies. Historically, hMSCs have been always considered a sub-population of mononuclear cells present in the bone marrow (BM). Although BM-hMSCs are s...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.24223
更新日期:2013-04-01 00:00:00
abstract::Diabetes mellitus (DM) induces bone deterioration, while mechanical stimulation promotes osteocyte-driven bone formation. We aimed to evaluate the interaction of acute exposure (24 h) to high glucose (HG) with both the pro-survival effect conferred to osteocytic MLO-Y4 cells and osteoblastic MC3T3-E1 cells by mechanic...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25829
更新日期:2017-12-01 00:00:00
abstract::Central (hypothalamic) control of bone mass is proposed to be mediated through beta2-adrenergic receptors (beta2-ARs). While investigations in mouse bone cells suggest that epinephrine enhances both RANKL and OPG mRNA via both beta-ARs and alpha-ARs, whether alpha-ARs are expressed in human bone cells is controversial...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21761
更新日期:2009-07-01 00:00:00
abstract::The Toll and Toll-like receptor signaling pathways are evolutionarily conserved pathways that regulate innate immunity in insects and mammals. While efforts have been made to clarify the signal transduction events that occur during infection, much less is known about the components that maintain immune quiescence. Her...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24472
更新日期:2014-04-01 00:00:00
abstract::The effects of P2Y2 purinoceptor activation on c-Fos expression and the signaling pathways evoked by extracellular ATP/UTP in HeLa cells were investigated. We found that P2Y2 activation induced c-Fos protein and phosphorylated the extracellular signal-regulated kinases 1 and 2 (ERK1/2). The P2Y2-stimulated c-Fos induc...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10242
更新日期:2003-05-01 00:00:00
abstract::Prostaglandin F2α (PGF2α) plays multiple roles on bone metabolism by regulating a wide range of signaling pathways. PGF2α, via activation of PKC, stimulates Na-dependent inorganic phosphate (Pi) transport system in osteoblasts; up-regulates interleukin (IL)-6 synthesis; increases vascular endothelial growth factor (VE...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.24117
更新日期:2013-01-01 00:00:00
abstract::Small intestinal crypt cells play a critical role in modulating Cl- secretion during digestion. The types of Cl- channels mediating Cl- secretion in the small intestine was investigated using the intestinal epithelial cell line, IEC-18, which was derived from rat small intestine crypt cells. In initial radioisotope ef...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20085
更新日期:2005-01-01 00:00:00
abstract::The stromal cell-derived factor-1 (SDF-1)/CXC receptor 4 (CXCR4) axis has been shown to play a role in colorectal cancer progression. In addition, the protease urokinase-type plasminogen activator (uPA) is an important factor in tumor cell invasion and metastasis. However, the mechanism by which SDF-1 mediates uPA exp...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22831
更新日期:2012-03-01 00:00:00
abstract::Low O(2) concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O(2), we studied its effects in cultures at 1% O(2). The pre-CFC activity in Lin- population of mouse bone marrow was analyzed...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21003
更新日期:2007-07-01 00:00:00
abstract::Di- and tripeptides and peptide mimetics such as beta-lactam antibiotics are efficiently reabsorbed from the tubular lumen by a high-affinity peptide transporter. We have recently identified and characterized this H+-coupled high-affinity peptide transport system in the porcine proximal tubular cell line LLC-PK1. Here...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199903)178:3<341::AID-JCP8
更新日期:1999-03-01 00:00:00
abstract::Programmed cell death in mammary tissue was studied during natural weaning in lactating mice and after litter removal or milk stasis. All treatments stimulated mammary apoptosis, indicating that this process is an integral part of the tissue's involution after lactation. Induction of apoptosis was slower in natural we...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199609)168:3<559::AID-JCP8
更新日期:1996-09-01 00:00:00
abstract::Immunotherapy has caused a paradigm shift in the treatment of several malignancies, particularly the blockade of programmed death-1 (PD-1) and its specific receptor/ligand PD-L1 that have revolutionized the treatment of a variety of malignancies, but significant durable responses only occur in a small percentage of pa...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.29491
更新日期:2020-07-01 00:00:00
abstract::We have described recently that expression of CD44 exon v10 (CD44v10) is down-regulated upon metastasis of squamous cell carcinoma, whereas it is up-regulated in skin metastases of malignant melanoma. The striking regulation of CD44v10 prompted us to generate a murine CD44v10-specific monoclonal antibody to define exp...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199706)171:3<305::AID-JCP9
更新日期:1997-06-01 00:00:00
abstract::Transient receptor potential vanilloid 4 (TRPV4) channels are mechanosensitive ion channels that regulate systemic endothelial cell (EC) functions such as vasodilation, permeability, and angiogenesis. TRPV4 is expressed in retinal ganglion cells, Müller glia, pigment epithelium, microvascular ECs, and modulates cell v...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30116
更新日期:2020-10-20 00:00:00
abstract::The secosteroid hormone 1alpha, 25-dihydroxyvitamin D3 (1,25D) has been shown to regulate the growth and differentiation of human prostate cancer (PCa) cells, although the precise molecular mechanisms mediating these effects have not been defined. Previous studies in our laboratory demonstrated that the antiproliferat...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20692
更新日期:2006-09-01 00:00:00
abstract::This study examines the mechanism by which TGF-beta 1, an important mediator of cell growth and differentiation, blocks the differentiation of normal rat diploid fetal osteoblasts in vitro. We have established that the inability for pre-osteoblasts to differentiate is associated with changes in the expression of cell ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041600214
更新日期:1994-08-01 00:00:00
abstract:BACKGROUND:In this study, we aimed to explore the effects of GAS5 single-nucleotide polymorphisms (SNPs) on GAS5 expression. And the signaling pathways underlying the function of GAS5 during the pathogenesis of prostate cancer (PC) were clarified. MATERIALS AND METHODS:Patients with PC were recruited and grouped accor...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27561
更新日期:2019-06-01 00:00:00
abstract::Numerous studies have suggested that urothelial cancer-associated 1 (UCA1) acts as a suppressor gene affecting cell proliferation and migration. However, the biological role and the potential mechanism of UCA1 in the progression of pre-eclampsia (PE) remains unclear. The UCA1 level was markedly upregulated in PE pregn...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29643
更新日期:2020-10-01 00:00:00
abstract::Neurite formation and proliferation by NG108-15 cells were studied in short-term serum-free N2 medium. Neuritogenesis by individual cells was observed at widely differing times, suggesting a stochastic component to initiation of differentiation. Cells with and without neurites could also enter one or more rounds of pr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041250222
更新日期:1985-11-01 00:00:00