Hemin promotes proliferation and differentiation of endothelial progenitor cells via activation of AKT and ERK.


:Increased neovascularization is commonly observed in hemorrhagic plaques and associated with rupture of atherosclerotic lesions. This study aims to investigate whether hemin accumulated at the site of hematoma promotes neovascularization through affecting the growth and function of endothelial progenitor cells (EPCs) and the possible mechanism involved. Here we demonstrated that hemin promoted a significant increase in neovessel formation in matrigel plugs embedded in vivo and enhanced the proliferation and endothelial gene expression in EPCs in vitro. VEGF-induced migration response and the capability to incorporate into the vascular networks were markedly enhanced in hemin-treated EPCs. Hemin induced the phosphorylation of ERK and AKT but not p38 or JNK. The inhibition of AKT or ERK activation significantly attenuated the effect of hemin on cell proliferation. However, the enhanced migration response induced by hemin was significantly suppressed by the inhibition of AKT but not ERK. Hemin induced significant increase in reactive oxygen species (ROS) production and hemin-induced angiogenic response of EPCs was substantially reduced by treatment with N-acetylcysteine. Collectively, these data support that hemin-induced ROS mediates the activation of AKT and ERK signaling pathways, which in turn promotes the cell proliferation and function of EPCs. J. Cell. Physiol. 219: 617-625, 2009. (c) 2009 Wiley-Liss, Inc.


J Cell Physiol


Wang JY,Lee YT,Chang PF,Chau LY




Has Abstract


2009-06-01 00:00:00












  • In vitro evaluation of different methods of handling human liposuction aspirate and their effect on adipocytes and adipose derived stem cells.

    abstract::Nowadays, fat tissue transplantation is widely used in regenerative and reconstructive surgery. However, a shared method of lipoaspirate handling for ensuring a good quality fat transplant has not yet been established. The study was to identify a method to recover from the lipoaspirate samples the highest number of hu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Palumbo P,Miconi G,Cinque B,La Torre C,Lombardi F,Zoccali G,Orsini G,Leocata P,Giuliani M,Cifone MG

    更新日期:2015-08-01 00:00:00

  • 15-Lipoxygenase promotes chronic hypoxia-induced phenotype changes of PASMCs via positive feedback-loop of BMP4.

    abstract::Our laboratory has previously demonstrated that 15-lipoxygenase (15-LO)/15-hydroxyeicosatetr-aenoic acid (15-HETE) is involved in hypoxic pulmonary arterial hypertension (PAH). Phenotypical alterations of vascular smooth muscle cells are considered to be an important stage in the development of PAH, whereas the underl...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yu X,Wei L,Lu P,Shen T,Liu X,Li T,Zhang B,Yu H,Zhu D

    更新日期:2015-07-01 00:00:00

  • Downregulation of uPAR promotes urokinase translocation into the nucleus and epithelial to mesenchymal transition in neuroblastoma.

    abstract::The urokinase system is involved in a variety of physiological processes, such as fibrinolysis, matrix remodeling, wound healing, and regeneration. Upon binding to its cognate receptor urokinase-type plasminogen activator receptor (uPAR), urokinase-type plasminogen activator (uPA) catalyzes the conversion of plasminog...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Semina EV,Rubina KA,Shmakova AA,Rysenkova KD,Klimovich PS,Aleksanrushkina NA,Sysoeva VY,Karagyaur MN,Tkachuk VA

    更新日期:2020-09-01 00:00:00

  • Regulation of self-renewal of human T lymphocyte colony-forming units (TL-CFUs).

    abstract::Formation of human T lymphocyte colonies in semisolid medium from T lymphocyte colony-forming units (TL-CFUs) under stimulation of phytohemagglutinin (PHA) has been reported by several authors. These TL-CFUs were present in unsensitized lymphocyte populations. We report here that such TL-CFUs are capable of renewing t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wu AM

    更新日期:1983-10-01 00:00:00

  • Tiliroside is a new potential therapeutic drug for osteoporosis in mice.

    abstract::Osteoporosis is a class of metabolic bone disease caused by complexed ramifications. Overactivation of osteoclasts due to a sudden decreased estrogen level plays a pivotal role for postmenopausal women suffering from osteoporosis. Therefore, inhibiting osteoclast formation and function has become a major direction for...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Li K,Xiao Y,Wang Z,Fu F,Shao S,Song F,Zhao J,Lin X,Liu Q,Xu J

    更新日期:2019-02-27 00:00:00

  • MiRNAs and inflammatory bowel disease: An interesting new story.

    abstract::Inflammatory bowel disease (IBD), as a chronic and recurrent inflammatory disorder, is caused by a dysregulated and aberrant immune response to exposed environmental factors in genetically susceptible individuals. Despite huge efforts in determining the molecular pathogenesis of IBD, an increasing worldwide incidence ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Moein S,Vaghari-Tabari M,Qujeq D,Majidinia M,Nabavi SM,Yousefi B

    更新日期:2019-04-01 00:00:00

  • Cellular control of ornithine decarboxylase activity by its antizyme.

    abstract::Conditions have been established under which the antizyme of ornithine decarboxylase (E.C., L-ornithine carboxy-lyase, ODC) a non-competitive protein inhibitor of ODC, can be detected in cells in response to as little as 10(-7) M putrescine. The maintenance of intracellular antizyme activity depends upon the ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Heller JS,Canellakis ES

    更新日期:1981-05-01 00:00:00

  • The development of lysosomal apparatus. II. Incorporation, subcellular distribution, and intraparticulate hydrolysis of 131 I-albumin by liver of mice at perinatal stages.

    abstract::Mouse fetuses at 15th and 18th day of gestation, and newborns aging 0, 5, 10, and 15 days were injected i.p. with 131 I-albumin (RISA). The degree of incorporation by liver and the intraparticulate hydrolysis of RISA in 27,000g x 10 min. particles increased after birth. By this time, changes in subcellular distributio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bertini F,Mayorga L,Gonzalez M

    更新日期:1981-11-01 00:00:00

  • LINC00261 suppresses human colon cancer progression via sponging miR-324-3p and inactivating the Wnt/β-catenin pathway.

    abstract::Growing evidence indicates long noncoding RNAs (lncRNAs) are significant regulators in the progression of various malignant tumors including colon cancer. Dysregulation of lncRNA LINC00261 has been identified in many cancers. Investigations on LINC00261 function have revealed that LINC00261 could act as a crucial tumo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yan D,Liu W,Liu Y,Luo M

    更新日期:2019-12-01 00:00:00

  • Interference with cellular differentiation by D-serine through antagonism at N-methyl-D-aspartate receptors composed of NR1 and NR3A subunits in chondrocytes.

    abstract::Serine racemase (SR) is responsible for the biosynthesis of D-serine (D-Ser), an endogenous co-agonist for the glycine (Gly)-binding site on N-methyl-D-aspartate (NMDA) receptors, from L-Ser in the brain. We have previously demonstrated high expression of SR by chondrocytes in cartilage. In this study, we attempted to...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Takarada T,Takahata Y,Iemata M,Hinoi E,Uno K,Hirai T,Yamamoto T,Yoneda Y

    更新日期:2009-09-01 00:00:00

  • Overexpression of basic fibroblast growth factor in MCF-7 human breast cancer cells: lack of correlation between inhibition of cell growth and MAP kinase activation.

    abstract::Basic fibroblast growth factor (bFGF, FGF-2) is progressively lost from mammary epithelial cells as they become malignant. To investigate the effects of restoring the expression of bFGF in breast cancer cells, we constructed MCF-7 cells that permanently overexpress 18-kD cytoplasm-localizing bFGF (MCF-7/deltaA(FGF)(18...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wieder R,Fenig E,Wang H,Wang Q,Paglin S,Menzel T,Gabrilove J,Fuks Z,Yahalom J

    更新日期:1998-12-01 00:00:00

  • The possible role of bacteria, viruses, and parasites in initiation and exacerbation of irritable bowel syndrome.

    abstract::Irritable bowel syndrome (IBS) is a prolonged and disabling functional gastrointestinal disorder with the incidence rate of 18% in the world. IBS could seriously affect lifetime of patients and cause high economic burden on the community. The pathophysiology of the IBS is hardly understood, whereas several possible me...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Shariati A,Fallah F,Pormohammad A,Taghipour A,Safari H,Chirani AS,Sabour S,Alizadeh-Sani M,Azimi T

    更新日期:2019-06-01 00:00:00

  • Biophysical properties of human astrocytic brain tumor cells in cell culture.

    abstract::For malignant cells cultured from a human astrocytoma, electrophysiological characteristics of the plasma membrane included specific resistivity of 446.82 +/- 279.5 ohm . cm2, specific capacitance of 0.758 +/- 0.52 microfarads/cm2, time constant 0.318 +/- 0.10 msec. The resting membrane potential averaged--14.07 +/- 7...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Black PM,Kornblith PL

    更新日期:1980-12-01 00:00:00

  • Serum stimulation of potassium fluxes, ouabain binding, and sodium fluxes in quiescent chicken embryo fibroblasts.

    abstract::Growth-contingent alterations in potassium and sodium fluxes, ouabain binding, and potassium ion content were examined following serum stimulation of quiescent, density-inhibited chicken embryo fibroblasts. Serum stimulation resulted in very rapid 1.5- to 1.8-fold increases in ouabain-sensitive potassium influx and le...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Johnson MA,Weber MJ

    更新日期:1980-05-01 00:00:00

  • Relationship of finite proliferative lifespan, senescence, and quiescence in human cells.

    abstract::Cell hybrids were formed between human diploid fibroblasts (HDF) and carcinogen-transformed HDF to determine the relationship among: (1) finite proliferative lifespan, which we define as an age-related failure of a population to achieve one population doubling in 4 weeks; (2) arrest in a senescent state, which we defi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Stein GH,Namba M,Corsaro CM

    更新日期:1985-03-01 00:00:00

  • A novel strategy of identifying circRNA biomarkers in cardiovascular disease by meta-analysis.

    abstract::Circular RNAs (circRNAs) are stable and abundantly expressed in vivo but are abnormally expressed in several diseases. This study aimed to identify circRNAs acting as potential biomarkers for cardiovascular disease (CVD). Research were retrieved from the articles published by September 2018 in eight databases to compa...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,meta分析,评审


    authors: Li JJ,Wang W,Wang XQ,He Y,Wang SS,Yan YX

    更新日期:2019-12-01 00:00:00

  • Hypoxia induces epidermal keratinocyte matrix metalloproteinase-9 secretion via the protein kinase C pathway.

    abstract::Hypoxia promotes keratinocyte migration on wound bed connective tissues and is a profound biological signal that transforms a basal keratinocyte, destined to differentiate, into a motile cell that is essential for re-epithelialization. In this study, we examined the effect of hypoxia on keratinocyte-derived collagenas...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: O'Toole EA,van Koningsveld R,Chen M,Woodley DT

    更新日期:2008-01-01 00:00:00

  • Modulation of the development of human monocyte-derived dendritic cells by lithium chloride.

    abstract::Lithium has been used or explored to treat psychiatric and neurodegenerative diseases that are frequently associated with an abnormal immune status. It is likely that lithium may work through modulation of immune responses in these patients. Because dendritic cells (DC) play a central role in regulating immune respons...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Liu KJ,Lee YL,Yang YY,Shih NY,Ho CC,Wu YC,Huang TS,Huang MC,Liu HC,Shen WW,Leu SJ

    更新日期:2011-02-01 00:00:00

  • Comparative proteomic profiling of human osteoblast-derived extracellular matrices identifies proteins involved in mesenchymal stromal cell osteogenic differentiation and mineralization.

    abstract::The extracellular matrix (ECM) is a dynamic component of tissue architecture that physically supports cells and actively influences their behavior. In the context of bone regeneration, cell-secreted ECMs have become of interest as they reproduce tissue-architecture and modulate the promising properties of mesenchymal ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Baroncelli M,van der Eerden BC,Kan YY,Alves RD,Demmers JA,van de Peppel J,van Leeuwen JP

    更新日期:2018-01-01 00:00:00

  • Impacts of epidural electrical stimulation on Wnt signaling, FAAH, and BDNF following thoracic spinal cord injury in rat.

    abstract::Electrical stimulation (ES) has been shown to improve some of impairments after spinal cord injury (SCI), but the underlying mechanisms remain unclear. The Wnt signaling pathways and the endocannabinoid system appear to be modulated in response to SCI. This study aimed to investigate the effect of ES therapy on the ac...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ghorbani M,Shahabi P,Karimi P,Soltani-Zangbar H,Morshedi M,Bani S,Jafarzadehgharehziaaddin M,Sadeghzadeh-Oskouei B,Ahmadalipour A

    更新日期:2020-12-01 00:00:00

  • PKCε regulates contraction-stimulated GLUT4 traffic in skeletal muscle cells.

    abstract::The signaling pathways that stimulate glucose uptake in response to muscle contraction are not well defined. Recently, we showed that carbachol, an acetylcholine analog, stimulates contraction of C2C12 myotube cultures and the rapid arrival of myc-epitope tagged GLUT4 glucose transporters at the cell surface. Here, we...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Niu W,Bilan PJ,Yu J,Gao J,Boguslavsky S,Schertzer JD,Chu G,Yao Z,Klip A

    更新日期:2011-01-01 00:00:00

  • Lactate and TGF-β antagonistically regulate inflammasome activation in the tumor microenvironment.

    abstract::The tumor microenvironment significantly affects tumor progression, and tumor cells can also remodel the tumor microenvironment through complex interaction. Inflammasomes are innate immune system receptors/sensors that regulate an inflammatory response mainly mediated by the nucleotide-binding oligomerization domain-l...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tu CE,Hu Y,Zhou P,Guo X,Gu C,Zhang Y,Li A,Liu S

    更新日期:2020-11-23 00:00:00

  • Topology of inositol lipid signal transduction in the nucleus.

    abstract::An increasing body of evidence shows that many of the key inositol lipids and enzymes responsible for their metabolism reside in nuclei. Moreover, the association of the nuclear phosphoinositide cycle with progression through the cell cycle and commitment toward differentiation has built a wider picture of the implica...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Maraldi NM,Zini N,Santi S,Manzoli FA

    更新日期:1999-11-01 00:00:00

  • Intracellular chloride regulates cell proliferation through the activation of stress-activated protein kinases in MKN28 human gastric cancer cells.

    abstract::Recently, we reported that reduction of intracellular Cl(-) concentration ([Cl(-)](i)) inhibited proliferation of MKN28 gastric cancer cells by diminishing the transition rate from G(1) to S cell-cycle phase through upregulation of p21, cyclin-dependent kinase inhibitor, in a p53-independent manner. However, it is sti...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ohsawa R,Miyazaki H,Niisato N,Shiozaki A,Iwasaki Y,Otsuji E,Marunaka Y

    更新日期:2010-06-01 00:00:00

  • Maturation-dependent regulation of protein kinase C activity by vitamin D3 metabolites in chondrocyte cultures.

    abstract::Vitamin D3 metabolites regulate the differentiation of chondrocytes isolated from the growth zone or resting zone of rat costochondral cartilage. Since some of the direct membrane effects of vitamin D metabolites are nongenomic, we hypothesized that protein kinase C (PKC) plays a role in signal transduction for these ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sylvia VL,Schwartz Z,Schuman L,Morgan RT,Mackey S,Gomez R,Boyan BD

    更新日期:1993-11-01 00:00:00

  • Annexin A1: novel roles in skeletal muscle biology.

    abstract::Annexin A1 (ANXA1, lipocortin-1) is the first characterized member of the annexin superfamily of proteins, so called since their main property is to bind (i.e., to annex) to cellular membranes in a Ca(2+) -dependent manner. ANXA1 has been involved in a broad range of molecular and cellular processes, including anti-in...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Bizzarro V,Petrella A,Parente L

    更新日期:2012-08-01 00:00:00

  • Versatile role of curcumin and its derivatives in lung cancer therapy.

    abstract::Lung cancer is a main cause of death all over the world with a high incidence rate. Metastasis into neighboring and distant tissues as well as resistance of cancer cells to chemotherapy demand novel strategies in lung cancer therapy. Curcumin is a naturally occurring nutraceutical compound derived from Curcuma longa (...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Ashrafizadeh M,Najafi M,Makvandi P,Zarrabi A,Farkhondeh T,Samarghandian S

    更新日期:2020-12-01 00:00:00

  • Hydrocortisone regulation of hyaluronan metabolism in human skin organ culture.

    abstract::We studied the influence of hydrocortisone (HC) on hyaluronan (HA) metabolism in explants of human skin, a model retaining normal three-dimensional architecture of dermal connective tissue and dynamic growth and stratification of epidermal keratinocytes. The synthesis of hyaluronan and proteoglycans (PGs), and DNA, we...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Agren UM,Tammi M,Tammi R

    更新日期:1995-08-01 00:00:00

  • Contractile heterogeneity in ventricular myocardium.

    abstract::The transmural heterogeneity of the contractility in ventricular muscle has not been well-studied. Here, we investigated the calcium transient and sarcomere contraction/relaxation in the endocardial (Endo) and epicardial (Epi) myocytes. Endo and Epi myocytes were isolated from C57/BL6 mice by Langendorff perfusion. Ca...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Pan W,Yang Z,Cheng J,Qian C,Wang Y

    更新日期:2018-08-01 00:00:00

  • Calcium transport by sarcoplasmic reticulum of vascular smooth muscle: II. Effects of calmodulin and calmodulin inhibitors.

    abstract::The role of calmodulin (CaM) in modulating calcium (Ca) uptake by sarcoplasmic reticulum (SR) of vascular smooth muscle was studied in saponin skinned strips of rat caudal artery. Exogenous CaM concentrations ranging from 0.3-1.8 microM did not statistically change the steady state MgATP-dependent Ca content, the MgAT...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Stout MA,Silver PJ

    更新日期:1992-10-01 00:00:00