Abstract:
:It is presently unclear what role elevations in intracellular calcium concentration ([Ca2+]i) play in the control of monokine secretion, or whether such alterations underlie the ability of physiologic stimuli to induce production of these important signalling molecules. To address these issues, we have performed experiments in murine peritoneal macrophages to determine whether lipopolysaccharide (LPS) or interferon gamma (IFN-gamma) initiate production of the proinflammatory monokine interleukin 6 (IL-6) concomitant with elevations in [Ca2+]i and with kinetics similar to that seen with known Ca2+ mobilizing agents. Alterations in [Ca2+]i after treatment with LPS, IFN-gamma, platelet activating factor (PAF), or thapsigargin were measured by fluorimetric methods. These effects were compared with the ability of each to induce IL-6 mRNA expression as measured by semiquantitative reverse-transcribed polymerase chain reactions. We report that neither LPS nor IFN-gamma elicited detectable elevations in [Ca2+]i but that both up-regulated expression of IL-6 mRNA expression within 60 min. In contrast, experiments using either thapsigargin or PAF showed rapid and dramatic elevations in [Ca2+]i with marked increases in IL-6 mRNA expression, as quickly as 15 min after initial exposure. Elevations in mRNA encoding IL-6 by thapsigargin and PAF were found to occur in a dose-dependent manner, mirroring their ability to elicit elevations in [Ca2+]i. These data demonstrate that LPS and IFN-gamma induce IL-6 message expression by means of Ca2+-independent signalling pathways. Furthermore, Ca2+-mobilizing agents that evoke monokine message expression do so far more rapidly than do LPS or IFN-gamma. Taken in concert, these data are consistent with the hypothesis that multiple signalling pathways exist by which production of proinflammatory monokines are initiated.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Marriott I,Bost KL,Mason MJdoi
10.1002/(SICI)1097-4652(199811)177:2<232::AID-JCP5subject
Has Abstractpub_date
1998-11-01 00:00:00pages
232-40issue
2eissn
0021-9541issn
1097-4652pii
10.1002/(SICI)1097-4652(199811)177:2<232::AID-JCP5journal_volume
177pub_type
杂志文章abstract::Interleukin-34 (IL-34) shares a common receptor with macrophage colony-stimulating factor (M-CSF), and can bind to CSF-1R, induces lymphocytes differentiation, proliferation, and regulates the synthesis of inflammatory components. Recent findings reported aberrant expression of IL-34 in several autoimmune disorders, s...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28946
更新日期:2019-12-01 00:00:00
abstract::Proliferation of human skin fibroblasts was stimulated significantly by the presence of L-ascorbic acid 2-phosphate (Asc 2-P). The presence of Asc 2-P (0.1-1.0 mM) in the culture medium for 3 weeks enhanced the relative rate of collagen synthesis to total protein synthesis 2-fold as well as cell growth 4-fold. Coexist...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041380103
更新日期:1989-01-01 00:00:00
abstract::The transient nuclear localization of metallothionein during cell growth and differentiation may be related to the increased requirement of zinc for DNA synthesis, activation of metalloenzymes, and transcription factors. Treatment of 3T3-L1 fibroblasts with aphidicolin, an inhibitor of nuclear DNA synthesis, caused a ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(200005)183:2<247::AID-JCP1
更新日期:2000-05-01 00:00:00
abstract::The p21 activated kinases (Paks), an evolutionarily conserved family of serine/threonine kinases, are important for a variety of cellular functions including cell morphogenesis, motility, survival, mitosis, and angiogenesis. Paks are widely expressed in numerous tissues and are activated by growth factors and extracel...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.10167
更新日期:2002-11-01 00:00:00
abstract::The serum-free medium conditioned by the human colon cancer cell line HT-29 contains insulin-like growth factors (IGF) that are entirely complexed to binding proteins (IGF-BP). Gel filtration in acid conditions of the cell-conditioned medium permits separation of IGF-BP from two molecular forms of IGF of 15,000 and 7,...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041430302
更新日期:1990-06-01 00:00:00
abstract::Apoptosis is involved in both the cellular and humoral immune system destroying tumors. An apoptosis-inducing factor from HL-60 myeloid leukemia cells was obtained, purified, and sequenced. The protein found has been identified as a human complement factor B-derived fragment Bb, although it is known that factor B is a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/1097-4652(200011)185:2<280::AID-JCP13>3.0.
更新日期:2000-11-01 00:00:00
abstract::Syndecan-1 belongs to a family of transmembrane proteoglycans, acts as a coreceptor for growth factor binding, as well as cell-matrix and cell-cell interactions, and is induced in smooth muscle cells (SMCs) following balloon catheter injury. In this report, we investigated syndecan-1 expression in SMCs in response to ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20927
更新日期:2007-04-01 00:00:00
abstract:BACKGROUND:Pulmonary arterial hypertension (PAH) is characterized by the apoptosis resistance and hyperproliferation of pulmonary artery smooth muscle cells (PASMCs). Its pathogenesis has not been revealed. Here, we carried out experiments to investigate the functions of miR-140-5p and tumor necrosis factor-α (TNF-α). ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27642
更新日期:2019-06-01 00:00:00
abstract::The effects of three purified colony-stimulating factors (CSFs) with different specificities for the granulocyte (G) and macrophage (M) lineages (G-CSF, CSF-1 and GM-CSF) were studied in a serum-free clonal assay system. The results were compared with those obtained in similar cultures containing fetal calf serum (FCS...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041280214
更新日期:1986-08-01 00:00:00
abstract::Acute myeloid leukemia (AML) is the most common type of leukemia in adults. AML cells secrete angiogenic factors to remodel vasculature and acquire chemoresistance; however, antiangiogenic drugs are often ineffective in AML treatment. Cancer cell-derived exosomes can induce angiogenesis, but their role in vascular rem...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27735
更新日期:2019-07-01 00:00:00
abstract::A method for killing dividing cells (Puck and Kao, '67) was adapted for the elimination of dividing heart muscle cells (myoblasts) in cultures. We have used this method to demonstrate their presence and to estimate their number as well as the number of nondividing heart muscle cells (myocytes) in the neo-natal rat hea...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041050203
更新日期:1980-11-01 00:00:00
abstract::The role of platelet-derived growth factor (PDGF) in the control of smooth muscle cell (SMC) differentiation was explored in vitro by examining its effects on expression of the smooth muscle (SM) specific contractile protein SM alpha actin in cultured rat aortic SMC. Quiescent, postconfluent SMC express maximal levels...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041420325
更新日期:1990-03-01 00:00:00
abstract::Retinoic acid reduces the growth rate of mouse S91 melanoma cells in culture and increases the proportion of cells in the G1 phase of the cell cycle. Because of the integral role protein synthesis has been shown to play in growth control we studied the effect of retinoic acid on the protein synthesis machinery with a ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041130110
更新日期:1982-10-01 00:00:00
abstract::Respiratory tract epithelia are one type of tissue targeted by vitamin A. In this study the effects of vitamin A and its analogs (retinoids) on human bronchial epithelial (HBE) cells have been investigated in a serum-free hormone-supplemented medium. This serum-free medium, which was developed for the long-term cultiv...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041270110
更新日期:1986-04-01 00:00:00
abstract::Studies were conducted to explore hormonal and nutritional factors that might be involved in the regulation of retinol-binding protein (RBP) synthesis and secretion by the liver. The studies employed primary cultures of hepatocytes from normal rats. When cells were cultured in Dulbecco's modified Eagle's medium alone,...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041300103
更新日期:1987-01-01 00:00:00
abstract::Vero M3 cells, a line derived from the kidney of an African Green Monkey, display certain alterations in their protein synthetic apparatus as a function of time during a growth cycle. (Growth cycle here refers to exponential growth of unsynchronized cells in culture and their subsequent passage into the stationary pha...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040860104
更新日期:1975-08-01 00:00:00
abstract::The retinal pigment epithelium is a monolayer of highly specialized pigmented cells located between the neural retina and the Bruch's membrane of the choroid. RPE cells play a crucial role in the maintenance and function of the underlying photoreceptors. This study introduces a spontaneously arising human retinal pigm...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25729
更新日期:2017-10-01 00:00:00
abstract::The increasing rate of mortality and morbidity because of cardiac diseases has called for efficient therapeutic needs. With the advancement in cell-based therapies, stem cells are abundantly studied in this area. Nearly, all sources of stem cells are experimented to treat cardiac injuries. Tissue engineering has also ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28330
更新日期:2019-02-27 00:00:00
abstract::[3H]Ouabain specifically bound at sublethal concentrations to Na,K-ATPase on the surface of HeLa cells is taken up (internalized) by the cells at a rate of three membrane equivalents of labeled sites per generation. Immediately following a pulse label with the glycoside, codistribution of radioactivity with the surfac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041100114
更新日期:1982-01-01 00:00:00
abstract::Priapism, a prolonged penile erection in the absence of sexual arousal, is common among patients with sickle cell disease (SCD). Hypogonadism is also common in patients with SCD. While the administration of exogenous testosterone reverses hypogonadism, it is contraceptive. We hypothesized that the stimulation of endog...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30075
更新日期:2020-09-24 00:00:00
abstract::Acute lung injury (ALI) is a severe acute inflammatory reaction of the lungs caused by a variety of factors, which can lead to a high mortality rate. MicroRNAs are a novel therapeutic molecule that play a vital role in many diseases. However, its mechanism of action in lipopolysaccharide (LPS)-induced mouse ALI is not...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28850
更新日期:2019-12-01 00:00:00
abstract::We evaluated the role of cytoskeletal elements in the degradation of endogenous proteins via autophagy using biochemical and morphological techniques. In the absence of exogenous amino acids, degradation of endogenous proteins was enhanced in cultured normal rat kidney cells. This enhanced degradative state was accomp...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041520304
更新日期:1992-09-01 00:00:00
abstract::Epithelial Cell Adhesion Molecule (EpCAM), or CD326, is a trans-membrane glycoprotein expressed by multiple normal epithelia as well as carcinoma. Human hepatic stem cells and bile duct epithelium of the liver are EpCAM positive. In tumor cell lines, its intracellular domain can be released after cleavage of the extra...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26286
更新日期:2018-06-01 00:00:00
abstract::A human osteosarcoma cell line, U-2 OS, cultured under serum-free conditions, was shown to produce a growth factor (osteosarcoma-derived growth factor, ODGF) for human-cultured glial cells, fibroblasts, and other cells. ODGF, collected from the spent medium of 2 OS cultures, was purified by a sequence involving hepari...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041050207
更新日期:1980-11-01 00:00:00
abstract::Zinc finger E-box binding homeobox 1 (ZEB1), an important transcription factor belonging to the ZEB family, plays a crucial role in regulating gene expression required for both normal physiological and pathological processes. Accumulating evidence has shown that ZEB1 participates in the initiation and progression of a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30177
更新日期:2020-12-04 00:00:00
abstract::So far, there were no reports on circular RNA (circRNA) expression profiles in the differentiation of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) into cardiomyocyte-like cells induced by 5-aza. In this study, hUCMSCs were isolated from umbilical cords and induced with 5-aza for 14 days. Immunofluores...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28310
更新日期:2019-02-12 00:00:00
abstract::Phosphodiesterase 5A (PDE5A) specifically degrades the ubiquitous second messenger cGMP and experimental and clinical data highlight its important role in cardiac diseases. To address PDE5A role in cardiac physiology, three splice variants of the PDE5A were cloned for the first time from mouse cDNA library (mPde5a1, m...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25880
更新日期:2018-01-01 00:00:00
abstract::Vitamin D3 metabolites regulate the differentiation of chondrocytes isolated from the growth zone or resting zone of rat costochondral cartilage. Since some of the direct membrane effects of vitamin D metabolites are nongenomic, we hypothesized that protein kinase C (PKC) plays a role in signal transduction for these ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041570209
更新日期:1993-11-01 00:00:00
abstract::The cancer stem cell (CSC) model encompasses an advantageous paradigm that in recent decades provides a better elucidation for many important biological aspects of cancer initiation, progression, metastasis, and, more important, development of multidrug resistance (MDR). Such several other hematological malignancies a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.26768
更新日期:2019-04-01 00:00:00
abstract::Serine racemase (SR) is responsible for the biosynthesis of D-serine (D-Ser), an endogenous co-agonist for the glycine (Gly)-binding site on N-methyl-D-aspartate (NMDA) receptors, from L-Ser in the brain. We have previously demonstrated high expression of SR by chondrocytes in cartilage. In this study, we attempted to...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21821
更新日期:2009-09-01 00:00:00