Abstract:
:The transient nuclear localization of metallothionein during cell growth and differentiation may be related to the increased requirement of zinc for DNA synthesis, activation of metalloenzymes, and transcription factors. Treatment of 3T3-L1 fibroblasts with aphidicolin, an inhibitor of nuclear DNA synthesis, caused a cell-cycle block at G1/S phase and a delay in the onset of M phase. This also resulted in the accumulation of both zinc and metallothionein in the nucleus. After removal of aphidicolin, the cells rapidly reentered S phase, and during the G2/M phase of cell cycle both zinc and metallothionein began to relocate to the cytoplasm. Delaying the onset of M phase in 3T3-L1 cells could prevent the cytoplasmic relocation of metallothionein. The nuclear translocation of both zinc and metallothionein during the cell cycle can be considered as a normal process and this may be a general mechanism in response to mitogenic signals.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Apostolova MD,Cherian MGdoi
10.1002/(SICI)1097-4652(200005)183:2<247::AID-JCP1keywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
247-53issue
2eissn
0021-9541issn
1097-4652pii
10.1002/(SICI)1097-4652(200005)183:2<247::AID-JCP1journal_volume
183pub_type
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